Objective: the purpose of this experiment is to study the effect of compound lizard powder gel on intestinal flora of rats with ulcerative colitis (UC). Methods: SPF male SD rats were randomly divided into 6 groups: b...Objective: the purpose of this experiment is to study the effect of compound lizard powder gel on intestinal flora of rats with ulcerative colitis (UC). Methods: SPF male SD rats were randomly divided into 6 groups: blank group, model control group, compound lizard powder gel high, middle and low groups and sulfasalazine tablet treatment group. The clinical behavior of rats was observed daily during the modeling of TNBS, modeling period and drug intervention treatment period, and 16S rDNA high-throughput sequencing technique was used to compare and analyze the flora structure and bacteria genus differences in fresh feces of six groups of rats. Results: clinical behavior observation showed that the clinical condition of rats in the blank group was always good. In the model group, some rats had soft stools on the first day of modeling, and their spirit, activity and hair gradually changed. By the third day, the symptoms of loose stools and bloody stools gradually worsened. After treatment, the clinical symptoms of each group changed, but each group had its own characteristics. The sequencing results show that the current sequencing depth can fully reflect the diversity of microorganisms contained in the sample;PCoA analysis showed that the percentages of PCA1 and PCA2 in the score chart explaining population variance were 46.92% and 10.05% respectively. Multi-dimensional analysis shows that compared with the model control group and the blank group, the difference of flora composition between compound lizard powder group and sulfasalazine tablet treatment group is smaller. Analysis of the community structure of intestinal contents showed that the highest abundance in each group of samples at phylum level were phylum sclerenchyma and phycobacterium. After 14 days of drug intervention, the abundance of sclerenchyma and Bacteroides in compound lizard powder group and sulfasalazine tablet treatment group increased, and the abundance of Proteobacteria decreased, especially in compound Lizard powder middle dose group. In the control group, the abundance of degenerated phylum increased significantly. Genus levels, the highest abundance in each group of samples were Muribaculaceae_ unclassified and Lactobacillus. After 14 days of drug intervention, the abundance of Ruminococcaceae_UCG?005 in compound lizard powder group and sulfasalazine tablet treatment group increased significantly, and the abundance of Bacteroides in model control group was significantly higher than that in blank control group and compound lizard powder treatment groups. The lactic acid bacteria in the middle dosage group of compound lizard powder were higher than those in the high and low dosage groups of compound lizard powder and sulfasalazine tablets group. Conclusion: compound lizard powder gel, especially in the middle dosage group, is more effective in adjusting the intestinal flora structure and bacteria genus difference of UC.展开更多
Anaerobic process of biohydrogen production was developed. There is a great deal of Lactobacillus bacteria in the activated sludge of biohydrogen reactor. The isolation and identification of different anaerobic bacter...Anaerobic process of biohydrogen production was developed. There is a great deal of Lactobacillus bacteria in the activated sludge of biohydrogen reactor. The isolation and identification of different anaerobic bacteria in the reactor is important for fermented biohydrogen production process by anaerobic digesting organic wastewater. Considering with the physiological and biochemical traits,morphological characteristics and 16SrDNA sequence,the isolated Rennanqilyf13 is a new species in Lactobacillus genus. And the temporary nomenclature of the species is Lactobacillus Strain Rennanqilyf13 sp. nov.展开更多
文摘Objective: the purpose of this experiment is to study the effect of compound lizard powder gel on intestinal flora of rats with ulcerative colitis (UC). Methods: SPF male SD rats were randomly divided into 6 groups: blank group, model control group, compound lizard powder gel high, middle and low groups and sulfasalazine tablet treatment group. The clinical behavior of rats was observed daily during the modeling of TNBS, modeling period and drug intervention treatment period, and 16S rDNA high-throughput sequencing technique was used to compare and analyze the flora structure and bacteria genus differences in fresh feces of six groups of rats. Results: clinical behavior observation showed that the clinical condition of rats in the blank group was always good. In the model group, some rats had soft stools on the first day of modeling, and their spirit, activity and hair gradually changed. By the third day, the symptoms of loose stools and bloody stools gradually worsened. After treatment, the clinical symptoms of each group changed, but each group had its own characteristics. The sequencing results show that the current sequencing depth can fully reflect the diversity of microorganisms contained in the sample;PCoA analysis showed that the percentages of PCA1 and PCA2 in the score chart explaining population variance were 46.92% and 10.05% respectively. Multi-dimensional analysis shows that compared with the model control group and the blank group, the difference of flora composition between compound lizard powder group and sulfasalazine tablet treatment group is smaller. Analysis of the community structure of intestinal contents showed that the highest abundance in each group of samples at phylum level were phylum sclerenchyma and phycobacterium. After 14 days of drug intervention, the abundance of sclerenchyma and Bacteroides in compound lizard powder group and sulfasalazine tablet treatment group increased, and the abundance of Proteobacteria decreased, especially in compound Lizard powder middle dose group. In the control group, the abundance of degenerated phylum increased significantly. Genus levels, the highest abundance in each group of samples were Muribaculaceae_ unclassified and Lactobacillus. After 14 days of drug intervention, the abundance of Ruminococcaceae_UCG?005 in compound lizard powder group and sulfasalazine tablet treatment group increased significantly, and the abundance of Bacteroides in model control group was significantly higher than that in blank control group and compound lizard powder treatment groups. The lactic acid bacteria in the middle dosage group of compound lizard powder were higher than those in the high and low dosage groups of compound lizard powder and sulfasalazine tablets group. Conclusion: compound lizard powder gel, especially in the middle dosage group, is more effective in adjusting the intestinal flora structure and bacteria genus difference of UC.
基金Shanghai Education Committee Foundation (Grant No.N07ZZ156 S0701004 P1402).
文摘Anaerobic process of biohydrogen production was developed. There is a great deal of Lactobacillus bacteria in the activated sludge of biohydrogen reactor. The isolation and identification of different anaerobic bacteria in the reactor is important for fermented biohydrogen production process by anaerobic digesting organic wastewater. Considering with the physiological and biochemical traits,morphological characteristics and 16SrDNA sequence,the isolated Rennanqilyf13 is a new species in Lactobacillus genus. And the temporary nomenclature of the species is Lactobacillus Strain Rennanqilyf13 sp. nov.
