<div style="text-align:justify;"> A generalized optical filterless approach to achieve photonic generation of frequency 16-tupling millimeter-wave (mm-wave) signal based on two cascaded dual-parallel M...<div style="text-align:justify;"> A generalized optical filterless approach to achieve photonic generation of frequency 16-tupling millimeter-wave (mm-wave) signal based on two cascaded dual-parallel Mach-Zehnder modulators (DPMZMs) is presented. A theoretical analysis leading to the operating conditions to achieve frequency 16-tupling is developed. Different modulation indices (MIs) can be implemented to achieve the frequency multiplication by adjusting the delay of tunable optical delay line (TODL). It is confirmed by simulation that the proposed scheme is effective, and the radio frequency spurious suppression ratio (RFSSR) of the generated frequency 16-tupling signal can be as high as 40 dB when the sub-MZMs have extinction ratios of 30 dB. Influencing factors such as extinction ratio, DC bias drift, phase shift deviation and RF voltage deviation on the performance of optical sideband suppression ratio (OSSR) and RFSSR are also investigated. </div>展开更多
目的探讨血清骨膜蛋白(POSTN)、微小核糖核酸-15b(miR-15b)、Clara细胞分泌蛋白16(CC16)与新生儿支气管肺发育不良(BPD)的相关性。方法选取2022年1月至2024年1月保定市妇幼保健院收治的BPD早产儿186例为BPD组,同期非BPD早产儿186例为非...目的探讨血清骨膜蛋白(POSTN)、微小核糖核酸-15b(miR-15b)、Clara细胞分泌蛋白16(CC16)与新生儿支气管肺发育不良(BPD)的相关性。方法选取2022年1月至2024年1月保定市妇幼保健院收治的BPD早产儿186例为BPD组,同期非BPD早产儿186例为非BPD组,比较两组血清POSTN、miR-15b、CC16水平,基线资料,肺功能指标。Spearman分析BPD患儿血清POSTN、miR-15b、CC16与BPD的相关性。多因素Logistic回归分析BPD患儿的影响因素。结果BPD组血清POSTN、miR-15b高于非BPD组,CC16低于非BPD组,差异有统计学意义(P<0.05)。BPD组50%潮气量时呼气流速(50%TEF)、千克体重潮气量(VT/kg)、达峰容积比(VPEF/VE)低于非BPD组,差异有统计学意义(P<0.05)。Spearman相关性分析显示,POSTN、miR-15b与5 min Apgar评分、1,25-(OH)2D3、50%TEF、VT/kg、VPEF/VE呈负相关,POSTN与机械通气时间、吸氧时间、最高氧浓度呈正相关,miR-15b与呼吸窘迫综合征、机械通气时间、吸氧时间、最高氧浓度呈正相关;CC16与机械通气时间、吸氧时间、最高氧浓度呈负相关,与5 min Apgar评分、1,25-(OH)2D3、50%TEF、VT/kg、VPEF/VE呈正相关(P<0.05)。Logistic回归分析结果显示,呼吸窘迫综合征、机械通气时间、吸氧时间、最高氧浓度、POSTN、miR-15b是BPD患儿的危险因素,5 min Apgar评分、1,25-(OH)2D3、50%TEF、VT/kg、VPEF/VE、CC16是BPD患儿的保护因素(P<0.05)。结论BPD患儿血清POSTN、miR-15b升高,CC16降低,其水平变化与BPD发生密切相关。展开更多
Wu et al recently applied multi-region 16S rRNA sequencing to characterize the gastric cancer microbiome,demonstrating improved taxonomic resolution and detection sensitivity over conventional single-region approaches...Wu et al recently applied multi-region 16S rRNA sequencing to characterize the gastric cancer microbiome,demonstrating improved taxonomic resolution and detection sensitivity over conventional single-region approaches.While the study represents a valuable methodological step forward,it remains limited by singlecenter design,lack of quantitative calibration,and insufficient control for contamination and inter-laboratory variability.This editorial critically appraises these methodological gaps and emphasizes that future efforts must focus on harmonized,consensus-driven workflows to ensure reproducibility and clinical reliability.The translational potential of multi-region 16S lies in moving from descriptive microbial profiling to actionable clinical integration,particularly for recurrence prediction,treatment-response monitoring,and perioperative complication risk assessment.By addressing these methodological,economic,and ethical challenges,the field can advance toward evidence-based and clinically deployable microbiome-guided precision oncology.展开更多
文摘<div style="text-align:justify;"> A generalized optical filterless approach to achieve photonic generation of frequency 16-tupling millimeter-wave (mm-wave) signal based on two cascaded dual-parallel Mach-Zehnder modulators (DPMZMs) is presented. A theoretical analysis leading to the operating conditions to achieve frequency 16-tupling is developed. Different modulation indices (MIs) can be implemented to achieve the frequency multiplication by adjusting the delay of tunable optical delay line (TODL). It is confirmed by simulation that the proposed scheme is effective, and the radio frequency spurious suppression ratio (RFSSR) of the generated frequency 16-tupling signal can be as high as 40 dB when the sub-MZMs have extinction ratios of 30 dB. Influencing factors such as extinction ratio, DC bias drift, phase shift deviation and RF voltage deviation on the performance of optical sideband suppression ratio (OSSR) and RFSSR are also investigated. </div>
文摘目的探讨血清骨膜蛋白(POSTN)、微小核糖核酸-15b(miR-15b)、Clara细胞分泌蛋白16(CC16)与新生儿支气管肺发育不良(BPD)的相关性。方法选取2022年1月至2024年1月保定市妇幼保健院收治的BPD早产儿186例为BPD组,同期非BPD早产儿186例为非BPD组,比较两组血清POSTN、miR-15b、CC16水平,基线资料,肺功能指标。Spearman分析BPD患儿血清POSTN、miR-15b、CC16与BPD的相关性。多因素Logistic回归分析BPD患儿的影响因素。结果BPD组血清POSTN、miR-15b高于非BPD组,CC16低于非BPD组,差异有统计学意义(P<0.05)。BPD组50%潮气量时呼气流速(50%TEF)、千克体重潮气量(VT/kg)、达峰容积比(VPEF/VE)低于非BPD组,差异有统计学意义(P<0.05)。Spearman相关性分析显示,POSTN、miR-15b与5 min Apgar评分、1,25-(OH)2D3、50%TEF、VT/kg、VPEF/VE呈负相关,POSTN与机械通气时间、吸氧时间、最高氧浓度呈正相关,miR-15b与呼吸窘迫综合征、机械通气时间、吸氧时间、最高氧浓度呈正相关;CC16与机械通气时间、吸氧时间、最高氧浓度呈负相关,与5 min Apgar评分、1,25-(OH)2D3、50%TEF、VT/kg、VPEF/VE呈正相关(P<0.05)。Logistic回归分析结果显示,呼吸窘迫综合征、机械通气时间、吸氧时间、最高氧浓度、POSTN、miR-15b是BPD患儿的危险因素,5 min Apgar评分、1,25-(OH)2D3、50%TEF、VT/kg、VPEF/VE、CC16是BPD患儿的保护因素(P<0.05)。结论BPD患儿血清POSTN、miR-15b升高,CC16降低,其水平变化与BPD发生密切相关。
文摘Wu et al recently applied multi-region 16S rRNA sequencing to characterize the gastric cancer microbiome,demonstrating improved taxonomic resolution and detection sensitivity over conventional single-region approaches.While the study represents a valuable methodological step forward,it remains limited by singlecenter design,lack of quantitative calibration,and insufficient control for contamination and inter-laboratory variability.This editorial critically appraises these methodological gaps and emphasizes that future efforts must focus on harmonized,consensus-driven workflows to ensure reproducibility and clinical reliability.The translational potential of multi-region 16S lies in moving from descriptive microbial profiling to actionable clinical integration,particularly for recurrence prediction,treatment-response monitoring,and perioperative complication risk assessment.By addressing these methodological,economic,and ethical challenges,the field can advance toward evidence-based and clinically deployable microbiome-guided precision oncology.
文摘目的探讨RUNX3、P16和DAPK基因启动子DNA甲基化与非小细胞肺癌(non-small cell lung cancer,NSCLC)化疗敏感性的关系。方法选取2023年2月至2025年2月于株洲市中心医院接受铂类化疗的初治晚期NSCLC患者116例为研究对象,根据化疗疗效将其分为敏感组(n=81)和耐受组(n=35)。比较两组患者的病灶组织标本RUNX3、P16、DAPK基因启动子DNA甲基化情况、基因转录水平绝对定量,采用受试者操作特征曲线(receiver operating characteristic curve,ROC曲线)评估RUNX3、P16、DAPK基因表达水平对铂类化疗耐药的预测价值。结果耐受组患者肺癌组织的RUNX3、P16和DAPK基因启动子DNA甲基化比例均显著高于敏感组(P<0.05)。耐受组患者肺癌组织的RUNX3、P16和DAPK基因转录水平均显著低于敏感组(P<0.05)。ROC曲线显示,肺癌组织RUNX3、P16、DAPK基因转录水平预测NSCLC患者化疗敏感的最佳截断值分别为2.93×10^(6)、5.22×10^(4)、6.420×10^(5),曲线下面积(area under the curve,AUC)分别为0.726、0.748、0.763。三者联合预测NSCLC患者化疗敏感的AUC为0.883,敏感度为81.82%、特异性为78.05%。结论RUNX3、P16、DAPK基因甲基化可能是晚期NSCLC患者铂类化疗耐药的原因之一,早期测定此类基因转录水平对预估化疗耐药、制定个体化治疗方案具有积极作用。
