In this study,a compact 16-channel integrated charge-sensitive preamplifier named the smart preamplifier(SPA)was developed to support the large-scale detector array used in modern nuclear physics experiments.Two types...In this study,a compact 16-channel integrated charge-sensitive preamplifier named the smart preamplifier(SPA)was developed to support the large-scale detector array used in modern nuclear physics experiments.Two types of SPA,namely SPA02 and SPA03(with external field effect transistor),have been manufactured to match silicon detectors with small and large capacitances,respectively.The characteristics of the SPA include fast response of typically less than 6 ns for pulse rising time and low equivalent noise of 1.5 keV at zero input capacitance.The energy sensitivity and pulse decay time can be easily adjusted by changing the feedback capacitance Cfand resistance Rfin various applications.A good energy resolution of 24.4 keV for 5.803-MeV alpha particles from 244 Cm was achieved using a small-sized Si-PIN detector;for the silicon strip detectors in the test with the alpha source,a typical energy resolution of 0.6–0.8%was achieved.The integrated SPA has been employed in several experiments of silicon strip detectors with hundreds of channels,and a good performance has been realized.展开更多
High precision time measurement is required in the readout of the neutron wall and TOF walls in the external target experiment of the Cooling Storage Ring(CSR) project in the Heavy Ion Research Facility in Lanzhou(HIR...High precision time measurement is required in the readout of the neutron wall and TOF walls in the external target experiment of the Cooling Storage Ring(CSR) project in the Heavy Ion Research Facility in Lanzhou(HIRFL).Considering the time walk correction,both time and charge are measured in the readout electronics.In this 16-channel measurement module,time and charge information are digitized by TDCs at the same time based on the Time-Over-Threshold(TOT) method;meanwhile,by employing high-density ASIC chips,the electronics complexity is effectively reduced.Test results indicate that this module achieves a time resolution better than 25 ps and a charge resolution better than 5%over the input amplitude range from 50 mV to 3V.展开更多
A 16-channel gated integrator(GI) module following semi-Gaussian shaping amplifiers was developed for high-resolution energy spectroscopy systems at Institute of Modern Physics,Chinese Academy of Sciences.This GI solv...A 16-channel gated integrator(GI) module following semi-Gaussian shaping amplifiers was developed for high-resolution energy spectroscopy systems at Institute of Modern Physics,Chinese Academy of Sciences.This GI solved the ballistic deficit problem by integrating the signal until all the charge was collected from the detector at counting rates up to 100 K.In this paper,a fast shaper was used to optimize the gate logic to ensure nonlinearity of the GI less than 0.17%;and a novel compensation approach,to reduce the charge injection from the switches in the GI; and a T-switch configuration,to eliminate leakage current in the reset switch.展开更多
A CMOS front-end integrated circuit consisting of 16 identical analog channels is proposed for semiconductor radiation detectors. Each of the 16 channels has a low noise charge sensitive amplifier, a pulse shaper, a p...A CMOS front-end integrated circuit consisting of 16 identical analog channels is proposed for semiconductor radiation detectors. Each of the 16 channels has a low noise charge sensitive amplifier, a pulse shaper, a peak detect and hold circuit and a discriminator, while analog voltage and channel address are routed off the chip. It can accommodate both electron and hole collection with selectable gain and peaking time. Sequential and sparse readout, combining with self-trigger and external trigger, makes four readout modes. The circuit is implemented in a 0.35 μm DP4M (double-poly-quad-metal) CMOS technology with an area of 2.5×1.54 mm2 and power dissipation of 60 mW. A single channel chip is tested with Verigy 93000. The gain is adjustable from 13 to 130 mV·fC–1 while the peaking time varies between 0.7 and 1.6 μs. The linearity is more than 99% and the equivalent noise charge is about 600e.展开更多
干扰素-γ诱导蛋白16(interferon gamma-inducible protein 16,IFI16)是含pyrin和造血表达、干扰素诱导特性和核定位(hematopoietic expression,interferon-inducible nature,and nuclear localization,HIN)结构域的蛋白质(pyrin and HI...干扰素-γ诱导蛋白16(interferon gamma-inducible protein 16,IFI16)是含pyrin和造血表达、干扰素诱导特性和核定位(hematopoietic expression,interferon-inducible nature,and nuclear localization,HIN)结构域的蛋白质(pyrin and HIN domain-containing protein,PYHIN)家族的重要成员,其独特的分子结构使其能够识别细胞内的多种核酸分子。作为一种关键的免疫调节因子,IFI16可通过多种途径参与天然免疫信号转导,在宿主抗病毒防御中发挥重要作用。本文综述了IFI16的分子特征及其在天然免疫和病毒感染中的调控机制,为抗病毒感染的治疗靶点及药物开发提供理论依据。展开更多
目的 探究16S rDNA不同区间对药品生产环境常见细菌的种水平鉴定能力。方法 计算德国细菌古菌分类学数据库(List of Prokaryotic names with Standing in Nomenclature,LPSN)收录的所有模式菌株序列以及洁净室中常见分离细菌对应的模式...目的 探究16S rDNA不同区间对药品生产环境常见细菌的种水平鉴定能力。方法 计算德国细菌古菌分类学数据库(List of Prokaryotic names with Standing in Nomenclature,LPSN)收录的所有模式菌株序列以及洁净室中常见分离细菌对应的模式菌株在16S rDNA不同区间香农熵,分析16S rDNA不同区间的物种水平鉴定能力;对所有LPSN收录的模式菌株进行相似度差值的比较,同时收集403个具有代表性的药品生产环境分离菌株样品,进行16S rDNA测序后完成相似度差值的比较,基于相似度差值探究16S rDNA不同区间的鉴定结果区分度;考虑到不同区间对不同种属可能存在区分能力的差异,对各区间测序区分度不显著的物种进行了相关性分析。结果 16S rDNA的27F~533R区间相比其他区间(533F~1100R区间、1100F~1492R区间)具有最高的种间分辨率,同时该区间的鉴定结果中,第一顺位与第二顺位的序列相似度差值最大,扩展比对区间对提升物种间的区分度没有显著效果。结论 优先应用16S rDNA的27F~533R区间开展细菌的种水平鉴定是高效、可靠的做法。展开更多
The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a mol...The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a molding process,which enables the batch detection of pathogens.It explores the rapid lysis and elution processes of pathogens within the microfluidic chips to ensure that nucleic acid extraction,elution,and amplification are completed entirely within the chip.This chip can extract nucleic acids from samples in just 10 min,achieving an extraction efficiency comparable to that of traditional in-tube methods.An oil phase is pre-loaded into the chip to effectively prevent aerosol contamination.This approach allows for the simultaneous detection of 21 common respiratory pathogens,with a detection limit of 10 copies per reaction.Furthermore,applications involving clinical samples demonstrate significant practicality.Compared to many traditional in-tube pathogen detection methods and molecular biology technologies that utilize microfluidic chips,this detection chip not only enables simultaneous detection of multiple pathogens but also demonstrates high sensitivity.展开更多
基金supported by the National Key R&D Program of China(No.2018YFA0404404)the National Natural Science Foundation of China(Nos.11635015,U1732145,11705285,11805280,U1867212,and 11961131012)the Continuous Basic Scientific Research Project(No.WDJC-2019-13).
文摘In this study,a compact 16-channel integrated charge-sensitive preamplifier named the smart preamplifier(SPA)was developed to support the large-scale detector array used in modern nuclear physics experiments.Two types of SPA,namely SPA02 and SPA03(with external field effect transistor),have been manufactured to match silicon detectors with small and large capacitances,respectively.The characteristics of the SPA include fast response of typically less than 6 ns for pulse rising time and low equivalent noise of 1.5 keV at zero input capacitance.The energy sensitivity and pulse decay time can be easily adjusted by changing the feedback capacitance Cfand resistance Rfin various applications.A good energy resolution of 24.4 keV for 5.803-MeV alpha particles from 244 Cm was achieved using a small-sized Si-PIN detector;for the silicon strip detectors in the test with the alpha source,a typical energy resolution of 0.6–0.8%was achieved.The integrated SPA has been employed in several experiments of silicon strip detectors with hundreds of channels,and a good performance has been realized.
基金Supported by the Knowledge Innovation Program of the Chinese Academy of Sciences(No.KJCX2-YW-N27)the National Natural Science Foundation of China(No.11079003)
文摘High precision time measurement is required in the readout of the neutron wall and TOF walls in the external target experiment of the Cooling Storage Ring(CSR) project in the Heavy Ion Research Facility in Lanzhou(HIRFL).Considering the time walk correction,both time and charge are measured in the readout electronics.In this 16-channel measurement module,time and charge information are digitized by TDCs at the same time based on the Time-Over-Threshold(TOT) method;meanwhile,by employing high-density ASIC chips,the electronics complexity is effectively reduced.Test results indicate that this module achieves a time resolution better than 25 ps and a charge resolution better than 5%over the input amplitude range from 50 mV to 3V.
基金Supported by the Important Direction Project of the CAS Knowledge Innovation Program(No.KJCX2-YW-N27)the National Natural Science Foundation of China(No.11005135)
文摘A 16-channel gated integrator(GI) module following semi-Gaussian shaping amplifiers was developed for high-resolution energy spectroscopy systems at Institute of Modern Physics,Chinese Academy of Sciences.This GI solved the ballistic deficit problem by integrating the signal until all the charge was collected from the detector at counting rates up to 100 K.In this paper,a fast shaper was used to optimize the gate logic to ensure nonlinearity of the GI less than 0.17%;and a novel compensation approach,to reduce the charge injection from the switches in the GI; and a T-switch configuration,to eliminate leakage current in the reset switch.
基金Supported by the National Natural Science Foundation of China (No.40704025)
文摘A CMOS front-end integrated circuit consisting of 16 identical analog channels is proposed for semiconductor radiation detectors. Each of the 16 channels has a low noise charge sensitive amplifier, a pulse shaper, a peak detect and hold circuit and a discriminator, while analog voltage and channel address are routed off the chip. It can accommodate both electron and hole collection with selectable gain and peaking time. Sequential and sparse readout, combining with self-trigger and external trigger, makes four readout modes. The circuit is implemented in a 0.35 μm DP4M (double-poly-quad-metal) CMOS technology with an area of 2.5×1.54 mm2 and power dissipation of 60 mW. A single channel chip is tested with Verigy 93000. The gain is adjustable from 13 to 130 mV·fC–1 while the peaking time varies between 0.7 and 1.6 μs. The linearity is more than 99% and the equivalent noise charge is about 600e.
文摘干扰素-γ诱导蛋白16(interferon gamma-inducible protein 16,IFI16)是含pyrin和造血表达、干扰素诱导特性和核定位(hematopoietic expression,interferon-inducible nature,and nuclear localization,HIN)结构域的蛋白质(pyrin and HIN domain-containing protein,PYHIN)家族的重要成员,其独特的分子结构使其能够识别细胞内的多种核酸分子。作为一种关键的免疫调节因子,IFI16可通过多种途径参与天然免疫信号转导,在宿主抗病毒防御中发挥重要作用。本文综述了IFI16的分子特征及其在天然免疫和病毒感染中的调控机制,为抗病毒感染的治疗靶点及药物开发提供理论依据。
基金supported by grants from the National Key Research and Development Program of China(Nos.2023YFA0915200,2023YFA0915204)the Equipment Research and Development Projects of the Chinese Academy of Sciences(No.PTYQ2024YZ0010)+3 种基金the Science and Technology Commission of Shanghai Municipality Project(No.XTCX-KJ-2024-038)the Natural Science Foundation of Hebei Province of China(No.H2024206249)the Postdoctoral Fellowship Program of CPSF(No.GZC20232838)Science and Technology Commission of Shanghai Municipality(No.22S31901700).
文摘The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a molding process,which enables the batch detection of pathogens.It explores the rapid lysis and elution processes of pathogens within the microfluidic chips to ensure that nucleic acid extraction,elution,and amplification are completed entirely within the chip.This chip can extract nucleic acids from samples in just 10 min,achieving an extraction efficiency comparable to that of traditional in-tube methods.An oil phase is pre-loaded into the chip to effectively prevent aerosol contamination.This approach allows for the simultaneous detection of 21 common respiratory pathogens,with a detection limit of 10 copies per reaction.Furthermore,applications involving clinical samples demonstrate significant practicality.Compared to many traditional in-tube pathogen detection methods and molecular biology technologies that utilize microfluidic chips,this detection chip not only enables simultaneous detection of multiple pathogens but also demonstrates high sensitivity.
