报道了中国希瓦氏菌D14 T 的Fe(Ⅲ)还原特性,研究了溶氧浓度、光照强度、温度、pH等条件对菌株Fe(Ⅲ)还原的影响。结果发现,随着培养基中Fe(Ⅲ)浓度的提高,菌株D14 T 的Fe(Ⅲ)还原速率相应降低;氧气和光照对Fe(Ⅲ)还原有一定的抑制作用...报道了中国希瓦氏菌D14 T 的Fe(Ⅲ)还原特性,研究了溶氧浓度、光照强度、温度、pH等条件对菌株Fe(Ⅲ)还原的影响。结果发现,随着培养基中Fe(Ⅲ)浓度的提高,菌株D14 T 的Fe(Ⅲ)还原速率相应降低;氧气和光照对Fe(Ⅲ)还原有一定的抑制作用;菌株还原Fe(Ⅲ)的最适反应温度为37℃;在反应起始pH6 0 - 10 0的条件下菌株可进行Fe(Ⅲ)还原。对不同形态Fe(Ⅲ)还原特性的研究结果表明,Fe(Ⅲ)的溶解度越高越有利于还原反应的进行。采用SDS和OGP这两种蛋白变性剂对Fe(Ⅲ)还原蛋白进行初步定位的结果表明,参与Fe(Ⅲ)还原的蛋白主要位于细胞可溶性外周蛋白。在同时含有偶氮染料和Fe(Ⅲ)的条件下,菌株D14 T 的偶氮染料脱色率和Fe(Ⅲ)展开更多
目的:采用14条目D型人格量表(Type D Personality Scale-14,DS14)在医学生群体中进行检测,对比不同诊断标准下D型人格医学生的检出情况。方法:采用DS14对556名医学生的人格类型进行检测,分别统计不同诊断标准下D型人格医学生的检出率。...目的:采用14条目D型人格量表(Type D Personality Scale-14,DS14)在医学生群体中进行检测,对比不同诊断标准下D型人格医学生的检出情况。方法:采用DS14对556名医学生的人格类型进行检测,分别统计不同诊断标准下D型人格医学生的检出率。结果:原作检测标准下,D型人格医学生的检出率为仅为12.05%,明显低于国内相关外报道水平;调整检测标准后,D型人格医学生的检出率为28.24%%,与国内外经典报道基本一致。结论:适当调节D型人格医学生的诊断标准,可更加真实有效的对医学生群体中D型人格医学生的分布进行检测。展开更多
[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone ...[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana; then the amplified sequence was sequenced and homologically analyzed. [Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ) approaches. The full length of this cDNA was 866 bp encoding 197 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene (Ma-14-3-3d). Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species. [Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon.展开更多
通过简并引物和降落PCR方法结合RACE(rapid amplification of c DNA end)技术在香蕉果实c DNA文库中获得2个14-3-3基因,分别命名为Ma14-3-3d和Ma14-3-3h,把Ma14-3-3d和Ma14-3-3h与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i进行多重...通过简并引物和降落PCR方法结合RACE(rapid amplification of c DNA end)技术在香蕉果实c DNA文库中获得2个14-3-3基因,分别命名为Ma14-3-3d和Ma14-3-3h,把Ma14-3-3d和Ma14-3-3h与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i进行多重序列比对和同源性比较。结果表明:Ma14-3-3d和Ma14-3-3h与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i具有较高的同源性,同时具有一定的差异性。遗传进化分析结果表明,Ma14-3-3d与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i同属于non-ε类14-3-3基因,Ma14-3-3h属于ε类14-3-3基因。RT-PCR分析结果表明,Ma14-3-3d和Ma14-3-3h在香蕉不同器官中差异表达,Ma14-3-3d在香蕉的根、茎、叶、花和果中均有表达,且在茎、叶和花中的表达量高于根和果;Ma14-3-3h在根、花和果中的表达量较高,而在茎和叶中的表达量较低。q RT-PCR分析结果表明,Ma14-3-3d基因的表达明显受乙烯的诱导,而Ma14-3-3h在正常成熟、乙烯处理与1-MCP处理的果实中表达量均很低,与果实成熟的相关性不明显。推测Ma14-3-3d可能与香蕉果实成熟密切相关,可能参与乙烯调控果实成熟过程中的生物合成与信号转导。展开更多
Background: Periodontal infections are the most common disease of cats and dogs. About 70% - 80% of them suffer from these infections during their lifetime. These infections occur in two forms, gingivitis and periodon...Background: Periodontal infections are the most common disease of cats and dogs. About 70% - 80% of them suffer from these infections during their lifetime. These infections occur in two forms, gingivitis and periodontitis. Gingivitis is a reversible inflammation of the gingiva which can be cured. As with humans, the harmful bacteria can cause infections also in organs such as the heart, liver and kidneys. Besides veterinary treatment, also the owner’s everyday care is essential. Objective: The aim of the study was to develop and clinically test easy home care probiotic product for cats’ and dogs’ oral health. Methods: Nine cats and thirteen dogs were given a combination of selected bacteria Streptococcus thermophilus SP4, Lactobacillus plantarum 14D and Lactobacillus rhamnosus SP1 (Petguide powder) in powder form daily for four weeks on top of the dry feed. Microbiological samples were taken before and after the intervention from each dog and cat. The samples were analyzed with flow cytometric analysis using Porphyromonas gingivalis and Fusobacterium sp. as indicators for oral health. Results: The home care treatment with these specific, live probiotic bacteria was shown to prevent the growth of infectious bacteria within four weeks. Petguide probiotic powder reduced the relative abundance of infectious microbes significantly (p = 0.005) in cats and dogs. Conclusion: The results suggest that the probiotic product with three live strains of probiotic bacteria prevents the growth of infectious bacterial species that are known to cause infections in pets’ mouths. Therefore, this probiotic combination could be used to improve the oral health of pets and increase their quality of life.展开更多
文摘报道了中国希瓦氏菌D14 T 的Fe(Ⅲ)还原特性,研究了溶氧浓度、光照强度、温度、pH等条件对菌株Fe(Ⅲ)还原的影响。结果发现,随着培养基中Fe(Ⅲ)浓度的提高,菌株D14 T 的Fe(Ⅲ)还原速率相应降低;氧气和光照对Fe(Ⅲ)还原有一定的抑制作用;菌株还原Fe(Ⅲ)的最适反应温度为37℃;在反应起始pH6 0 - 10 0的条件下菌株可进行Fe(Ⅲ)还原。对不同形态Fe(Ⅲ)还原特性的研究结果表明,Fe(Ⅲ)的溶解度越高越有利于还原反应的进行。采用SDS和OGP这两种蛋白变性剂对Fe(Ⅲ)还原蛋白进行初步定位的结果表明,参与Fe(Ⅲ)还原的蛋白主要位于细胞可溶性外周蛋白。在同时含有偶氮染料和Fe(Ⅲ)的条件下,菌株D14 T 的偶氮染料脱色率和Fe(Ⅲ)
文摘目的:采用14条目D型人格量表(Type D Personality Scale-14,DS14)在医学生群体中进行检测,对比不同诊断标准下D型人格医学生的检出情况。方法:采用DS14对556名医学生的人格类型进行检测,分别统计不同诊断标准下D型人格医学生的检出率。结果:原作检测标准下,D型人格医学生的检出率为仅为12.05%,明显低于国内相关外报道水平;调整检测标准后,D型人格医学生的检出率为28.24%%,与国内外经典报道基本一致。结论:适当调节D型人格医学生的诊断标准,可更加真实有效的对医学生群体中D型人格医学生的分布进行检测。
文摘[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana; then the amplified sequence was sequenced and homologically analyzed. [Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ) approaches. The full length of this cDNA was 866 bp encoding 197 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene (Ma-14-3-3d). Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species. [Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon.
文摘通过简并引物和降落PCR方法结合RACE(rapid amplification of c DNA end)技术在香蕉果实c DNA文库中获得2个14-3-3基因,分别命名为Ma14-3-3d和Ma14-3-3h,把Ma14-3-3d和Ma14-3-3h与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i进行多重序列比对和同源性比较。结果表明:Ma14-3-3d和Ma14-3-3h与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i具有较高的同源性,同时具有一定的差异性。遗传进化分析结果表明,Ma14-3-3d与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i同属于non-ε类14-3-3基因,Ma14-3-3h属于ε类14-3-3基因。RT-PCR分析结果表明,Ma14-3-3d和Ma14-3-3h在香蕉不同器官中差异表达,Ma14-3-3d在香蕉的根、茎、叶、花和果中均有表达,且在茎、叶和花中的表达量高于根和果;Ma14-3-3h在根、花和果中的表达量较高,而在茎和叶中的表达量较低。q RT-PCR分析结果表明,Ma14-3-3d基因的表达明显受乙烯的诱导,而Ma14-3-3h在正常成熟、乙烯处理与1-MCP处理的果实中表达量均很低,与果实成熟的相关性不明显。推测Ma14-3-3d可能与香蕉果实成熟密切相关,可能参与乙烯调控果实成熟过程中的生物合成与信号转导。
文摘Background: Periodontal infections are the most common disease of cats and dogs. About 70% - 80% of them suffer from these infections during their lifetime. These infections occur in two forms, gingivitis and periodontitis. Gingivitis is a reversible inflammation of the gingiva which can be cured. As with humans, the harmful bacteria can cause infections also in organs such as the heart, liver and kidneys. Besides veterinary treatment, also the owner’s everyday care is essential. Objective: The aim of the study was to develop and clinically test easy home care probiotic product for cats’ and dogs’ oral health. Methods: Nine cats and thirteen dogs were given a combination of selected bacteria Streptococcus thermophilus SP4, Lactobacillus plantarum 14D and Lactobacillus rhamnosus SP1 (Petguide powder) in powder form daily for four weeks on top of the dry feed. Microbiological samples were taken before and after the intervention from each dog and cat. The samples were analyzed with flow cytometric analysis using Porphyromonas gingivalis and Fusobacterium sp. as indicators for oral health. Results: The home care treatment with these specific, live probiotic bacteria was shown to prevent the growth of infectious bacteria within four weeks. Petguide probiotic powder reduced the relative abundance of infectious microbes significantly (p = 0.005) in cats and dogs. Conclusion: The results suggest that the probiotic product with three live strains of probiotic bacteria prevents the growth of infectious bacterial species that are known to cause infections in pets’ mouths. Therefore, this probiotic combination could be used to improve the oral health of pets and increase their quality of life.
