BACKGROUND Kirsten rat sarcoma viral oncogene homolog(KRAS)is a commonly identified oncogenic driver in solid tumors,especially in non-small cell lung cancer.Until recently,KRAS was believed to be impossible to target...BACKGROUND Kirsten rat sarcoma viral oncogene homolog(KRAS)is a commonly identified oncogenic driver in solid tumors,especially in non-small cell lung cancer.Until recently,KRAS was believed to be impossible to target because it lacks adenosine triphosphate-binding domains or other regions that allow specic small-molecule inhibitors to act.In this report,we described using KRAS at glycine 12 to cysteine(G12C)inhibitors as posterior line therapy in a patient with relapsed metastatic lung adenocarcinoma carrying KRAS G12C mutation.CASE SUMMARY A 53-year-old Chinese man was treated with radical surgical resection for lung cancer in June 2014.Re-examination in June 2015 indicated postoperative rec-urrence with metastasis.The patient completed several courses of antitumor therapy,including pemetrexed and nedaplatin,bevacizumab with docetaxel and cisplatin,bevacizumab and pemetrexed,sintilimab and anlotinib,sintilimab and albumin-bound paclitaxel,and cadonilimab and docetaxel.In early May 2023,the patient developed a cough productive of bloody sputum and shortness of breath after exercise.The main adverse reactions associated with KRAS G12C inhibitor therapy were gastrointestinal reactions,which could be alleviated by daily oral ondansetron tablets.CONCLUSION After multiple-line treatment including chemotherapy,targeted therapy,and immunotherapy,disease control was achieved in a case of advanced pulmonary adenocarcinoma carrying the KRAS G12C mutation by mutation-specific inhibitor therapy,and the adverse reactions to the therapy were tolerable.展开更多
根据番茄表达数据库(http://ted.bti.cornell.edu/cgi-bin/)及实时定量分析发现,糖转运蛋白(sugars will eventually be exported transporters)基因SlSWEET12c在番茄感病品种和抗病品种中表达差异最明显。以番茄(Solanum lycopersicum...根据番茄表达数据库(http://ted.bti.cornell.edu/cgi-bin/)及实时定量分析发现,糖转运蛋白(sugars will eventually be exported transporters)基因SlSWEET12c在番茄感病品种和抗病品种中表达差异最明显。以番茄(Solanum lycopersicum)栽培品种‘中蔬6号’为试材,获得了稳定遗传的SlSWEET12c沉默(RNAi)和过表达(OE)植株。进一步接种细菌性叶斑病病菌丁香假单胞菌(Pst DC3000)后,通过对叶片中细菌生长量、Fv/Fm变化,可溶性糖和淀粉含量、活性氧积累及防御酶活性和PR蛋白相关基因表达情况的分析,明确SWEET12c在番茄抵御PstDC3000过程中的作用。与野生型相比,RNAiSlSWEET12c植株接种Pst DC3000后叶片中的细菌生长量、病斑数明显减少,对Pst DC3000的抗性增强,而OE-SlSWEET12c的叶片发病更严重,细菌菌落数及褐色病斑数增多。RNAi-SlSWEET12c植株在接菌后蔗糖和淀粉含量明显高于野生型,活性氧积累少于野生型,防御酶(POD、SOD、APX)活性和PR蛋白相关基因(NPR1、PR1、PR2、PR5)表达明显升高,而OE-SlSWEET12c植株蔗糖和淀粉含量低于野生型,活性氧积累高于野生型,防御酶活性和PR蛋白相关基因表达明显低于野生型。以上结果表明SlSWEET12c通过影响叶片中糖分的组成,负向调控番茄对细菌性叶斑病的抗性。展开更多
基金Supported by National Natural Sciences Foundation of China,No.81803553.
文摘BACKGROUND Kirsten rat sarcoma viral oncogene homolog(KRAS)is a commonly identified oncogenic driver in solid tumors,especially in non-small cell lung cancer.Until recently,KRAS was believed to be impossible to target because it lacks adenosine triphosphate-binding domains or other regions that allow specic small-molecule inhibitors to act.In this report,we described using KRAS at glycine 12 to cysteine(G12C)inhibitors as posterior line therapy in a patient with relapsed metastatic lung adenocarcinoma carrying KRAS G12C mutation.CASE SUMMARY A 53-year-old Chinese man was treated with radical surgical resection for lung cancer in June 2014.Re-examination in June 2015 indicated postoperative rec-urrence with metastasis.The patient completed several courses of antitumor therapy,including pemetrexed and nedaplatin,bevacizumab with docetaxel and cisplatin,bevacizumab and pemetrexed,sintilimab and anlotinib,sintilimab and albumin-bound paclitaxel,and cadonilimab and docetaxel.In early May 2023,the patient developed a cough productive of bloody sputum and shortness of breath after exercise.The main adverse reactions associated with KRAS G12C inhibitor therapy were gastrointestinal reactions,which could be alleviated by daily oral ondansetron tablets.CONCLUSION After multiple-line treatment including chemotherapy,targeted therapy,and immunotherapy,disease control was achieved in a case of advanced pulmonary adenocarcinoma carrying the KRAS G12C mutation by mutation-specific inhibitor therapy,and the adverse reactions to the therapy were tolerable.
文摘根据番茄表达数据库(http://ted.bti.cornell.edu/cgi-bin/)及实时定量分析发现,糖转运蛋白(sugars will eventually be exported transporters)基因SlSWEET12c在番茄感病品种和抗病品种中表达差异最明显。以番茄(Solanum lycopersicum)栽培品种‘中蔬6号’为试材,获得了稳定遗传的SlSWEET12c沉默(RNAi)和过表达(OE)植株。进一步接种细菌性叶斑病病菌丁香假单胞菌(Pst DC3000)后,通过对叶片中细菌生长量、Fv/Fm变化,可溶性糖和淀粉含量、活性氧积累及防御酶活性和PR蛋白相关基因表达情况的分析,明确SWEET12c在番茄抵御PstDC3000过程中的作用。与野生型相比,RNAiSlSWEET12c植株接种Pst DC3000后叶片中的细菌生长量、病斑数明显减少,对Pst DC3000的抗性增强,而OE-SlSWEET12c的叶片发病更严重,细菌菌落数及褐色病斑数增多。RNAi-SlSWEET12c植株在接菌后蔗糖和淀粉含量明显高于野生型,活性氧积累少于野生型,防御酶(POD、SOD、APX)活性和PR蛋白相关基因(NPR1、PR1、PR2、PR5)表达明显升高,而OE-SlSWEET12c植株蔗糖和淀粉含量低于野生型,活性氧积累高于野生型,防御酶活性和PR蛋白相关基因表达明显低于野生型。以上结果表明SlSWEET12c通过影响叶片中糖分的组成,负向调控番茄对细菌性叶斑病的抗性。
