An experimental muon source(EMuS) will be built at the China Spallation Neutron Source(CSNS). In phase I of CSNS, it has been decided that EMuS will provide a proton beam of 5 kW and 1.6 GeV to generate muon beams. A ...An experimental muon source(EMuS) will be built at the China Spallation Neutron Source(CSNS). In phase I of CSNS, it has been decided that EMuS will provide a proton beam of 5 kW and 1.6 GeV to generate muon beams. A 128-channel muon spin rotation/relaxation/resonance(μSR) spectrometer is proposed as a prototype surface muon spectrometer in a sub-branch of EMuS. The prototype spectrometer includes a detection system, sample environment, and supporting mechanics. The current design has two rings located at the forward and backward directions of the muon spin with 64 detectors per ring. The simulation shows that the highest asymmetry of approximately 0.28 is achieved by utilizing two 10-mm-thick brass degraders. To obtain the optimal asymmetry, the two-ring structure is updated to a four-ring structure with 32 segments in each ring. An asymmetry of 0.42 is obtained through the simulation, which is higher than that of all the current μSR spectrometers in the world.展开更多
The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a mol...The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a molding process,which enables the batch detection of pathogens.It explores the rapid lysis and elution processes of pathogens within the microfluidic chips to ensure that nucleic acid extraction,elution,and amplification are completed entirely within the chip.This chip can extract nucleic acids from samples in just 10 min,achieving an extraction efficiency comparable to that of traditional in-tube methods.An oil phase is pre-loaded into the chip to effectively prevent aerosol contamination.This approach allows for the simultaneous detection of 21 common respiratory pathogens,with a detection limit of 10 copies per reaction.Furthermore,applications involving clinical samples demonstrate significant practicality.Compared to many traditional in-tube pathogen detection methods and molecular biology technologies that utilize microfluidic chips,this detection chip not only enables simultaneous detection of multiple pathogens but also demonstrates high sensitivity.展开更多
目的分析冠状动脉病变患者血清微小RNA(miR)-128-3p,miRNA-126表达与Gensini积分的相关性。方法选取哈励逊国际和平医院心内科2020年6月~2022年接诊的60例冠状动脉病变患者展开回顾性研究,将其设为观察组;另选取该院同期接诊的60例非冠...目的分析冠状动脉病变患者血清微小RNA(miR)-128-3p,miRNA-126表达与Gensini积分的相关性。方法选取哈励逊国际和平医院心内科2020年6月~2022年接诊的60例冠状动脉病变患者展开回顾性研究,将其设为观察组;另选取该院同期接诊的60例非冠状动脉病变患者,将其设为对照组,检测、比较两组血清miR-128-3p,miR-126和Gensini积分;比较观察组中单支、双支、多支病变患者血清miR-128-3p,miR-126和Gensini积分;根据冠状动脉狭窄率分为Ⅰ度、Ⅱ度、Ⅲ度和Ⅳ度组,比较不同狭窄分级组血清miR-128-3p,miR-126和Gensini积分;根据Gensini积分分为轻度病变组和重度病变组,比较轻度病变组、重度病变组血清miR-128-3p,miR-126;Pearson分析血清miR-128-3p,miR-126与Gensini积分的相关性;绘制受试者工作(ROC)曲线,计算曲线下面积(AUC),分析血清miR-128-3p,miR-126联合检测对冠状动脉病变的预测效能。Logistic回归分析冠状动脉病变的影响因素。结果与对照组比较,观察组血清miR-128-3p(4.28±0.52 vs 2.61±0.36),Gensini积分(31.29±5.62分vs 6.16±1.04分)升高(t=21.678,34.058),血清miR-126(11.05±3.16 vs 19.62±5.14)降低(t=11.002),差异具有统计学意义(均P<0.05)。与双支病变、单支病变组比较,多支病变组血清miR-128-3p,Gensini积分升高(t=4.945,7.171;6.795,11.686),血清miR-126降低(t=3.104,5.033),差异具有统计学意义(均P<0.05)。与Ⅰ~Ⅱ级组比较,Ⅲ~Ⅳ级组血清miR-128-3p,Gensini积分升高(t=5.590,12.961),血清miR-126降低(t=6.021),差异具有统计学意义(均P<0.05)。与轻度病变组比较,重度病变组血清miR-128-3p升高(t=4.056),血清miR-126降低(t=4.806),差异具有统计学意义(均P<0.05)。血清miR-128-3p与Gensini积分呈正相关性(r=0.404,P<0.05),血清miR-126与Gensini积分呈负相关性(r=-0.393,P<0.05)。血清miR-128-3p,miR-126联合检测的AUC高于单一检测,差异具有统计学意义(Z=2.768,2.152,均P<0.05)。观察组吸烟、三酰甘油(TG)、血尿酸(UA)、纤维蛋白原(FIB)与对照组比较,差异具有统计学意义(t/χ^(2)=4.231~28.732,均P<0.05)。结论冠状动脉病变患者血清miR-128-3p呈高表达,血清miR-126呈低表达,与Gensini积分存在一定的相关性,血清miR-128-3p和miR-126联合检测可提高对冠状动脉病变的预测效能,且冠状动脉病变的发生与多种因素有关,应当引起临床重视。展开更多
基金supported by the National Natural Science Foundation of China(No.11527811)the Key Program of State Key Laboratory of Particle Detection and ElectronicsA part of the work performed in the UKRI ISIS Detector Group was sponsored by the China Scholarship Council
文摘An experimental muon source(EMuS) will be built at the China Spallation Neutron Source(CSNS). In phase I of CSNS, it has been decided that EMuS will provide a proton beam of 5 kW and 1.6 GeV to generate muon beams. A 128-channel muon spin rotation/relaxation/resonance(μSR) spectrometer is proposed as a prototype surface muon spectrometer in a sub-branch of EMuS. The prototype spectrometer includes a detection system, sample environment, and supporting mechanics. The current design has two rings located at the forward and backward directions of the muon spin with 64 detectors per ring. The simulation shows that the highest asymmetry of approximately 0.28 is achieved by utilizing two 10-mm-thick brass degraders. To obtain the optimal asymmetry, the two-ring structure is updated to a four-ring structure with 32 segments in each ring. An asymmetry of 0.42 is obtained through the simulation, which is higher than that of all the current μSR spectrometers in the world.
基金supported by grants from the National Key Research and Development Program of China(Nos.2023YFA0915200,2023YFA0915204)the Equipment Research and Development Projects of the Chinese Academy of Sciences(No.PTYQ2024YZ0010)+3 种基金the Science and Technology Commission of Shanghai Municipality Project(No.XTCX-KJ-2024-038)the Natural Science Foundation of Hebei Province of China(No.H2024206249)the Postdoctoral Fellowship Program of CPSF(No.GZC20232838)Science and Technology Commission of Shanghai Municipality(No.22S31901700).
文摘The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a molding process,which enables the batch detection of pathogens.It explores the rapid lysis and elution processes of pathogens within the microfluidic chips to ensure that nucleic acid extraction,elution,and amplification are completed entirely within the chip.This chip can extract nucleic acids from samples in just 10 min,achieving an extraction efficiency comparable to that of traditional in-tube methods.An oil phase is pre-loaded into the chip to effectively prevent aerosol contamination.This approach allows for the simultaneous detection of 21 common respiratory pathogens,with a detection limit of 10 copies per reaction.Furthermore,applications involving clinical samples demonstrate significant practicality.Compared to many traditional in-tube pathogen detection methods and molecular biology technologies that utilize microfluidic chips,this detection chip not only enables simultaneous detection of multiple pathogens but also demonstrates high sensitivity.
文摘目的分析冠状动脉病变患者血清微小RNA(miR)-128-3p,miRNA-126表达与Gensini积分的相关性。方法选取哈励逊国际和平医院心内科2020年6月~2022年接诊的60例冠状动脉病变患者展开回顾性研究,将其设为观察组;另选取该院同期接诊的60例非冠状动脉病变患者,将其设为对照组,检测、比较两组血清miR-128-3p,miR-126和Gensini积分;比较观察组中单支、双支、多支病变患者血清miR-128-3p,miR-126和Gensini积分;根据冠状动脉狭窄率分为Ⅰ度、Ⅱ度、Ⅲ度和Ⅳ度组,比较不同狭窄分级组血清miR-128-3p,miR-126和Gensini积分;根据Gensini积分分为轻度病变组和重度病变组,比较轻度病变组、重度病变组血清miR-128-3p,miR-126;Pearson分析血清miR-128-3p,miR-126与Gensini积分的相关性;绘制受试者工作(ROC)曲线,计算曲线下面积(AUC),分析血清miR-128-3p,miR-126联合检测对冠状动脉病变的预测效能。Logistic回归分析冠状动脉病变的影响因素。结果与对照组比较,观察组血清miR-128-3p(4.28±0.52 vs 2.61±0.36),Gensini积分(31.29±5.62分vs 6.16±1.04分)升高(t=21.678,34.058),血清miR-126(11.05±3.16 vs 19.62±5.14)降低(t=11.002),差异具有统计学意义(均P<0.05)。与双支病变、单支病变组比较,多支病变组血清miR-128-3p,Gensini积分升高(t=4.945,7.171;6.795,11.686),血清miR-126降低(t=3.104,5.033),差异具有统计学意义(均P<0.05)。与Ⅰ~Ⅱ级组比较,Ⅲ~Ⅳ级组血清miR-128-3p,Gensini积分升高(t=5.590,12.961),血清miR-126降低(t=6.021),差异具有统计学意义(均P<0.05)。与轻度病变组比较,重度病变组血清miR-128-3p升高(t=4.056),血清miR-126降低(t=4.806),差异具有统计学意义(均P<0.05)。血清miR-128-3p与Gensini积分呈正相关性(r=0.404,P<0.05),血清miR-126与Gensini积分呈负相关性(r=-0.393,P<0.05)。血清miR-128-3p,miR-126联合检测的AUC高于单一检测,差异具有统计学意义(Z=2.768,2.152,均P<0.05)。观察组吸烟、三酰甘油(TG)、血尿酸(UA)、纤维蛋白原(FIB)与对照组比较,差异具有统计学意义(t/χ^(2)=4.231~28.732,均P<0.05)。结论冠状动脉病变患者血清miR-128-3p呈高表达,血清miR-126呈低表达,与Gensini积分存在一定的相关性,血清miR-128-3p和miR-126联合检测可提高对冠状动脉病变的预测效能,且冠状动脉病变的发生与多种因素有关,应当引起临床重视。
