目的分析阿尔茨海默病(Alzheimer's disease,AD)患者血清叶酸、维生素B_(12)(VB_(12))、25羟维生素D[25-hydroxyvitamin D,25(OH)D]水平,并评估这3项指标与患者认知功能和日常生活能力的相关性及其诊断AD的效能。方法选取60例AD患者...目的分析阿尔茨海默病(Alzheimer's disease,AD)患者血清叶酸、维生素B_(12)(VB_(12))、25羟维生素D[25-hydroxyvitamin D,25(OH)D]水平,并评估这3项指标与患者认知功能和日常生活能力的相关性及其诊断AD的效能。方法选取60例AD患者(AD组)和48例体检健康老年人(对照组),检测两组血清叶酸、VB_(12)、25(OH)D水平。运用简易智力状态检查表(mini-mental state examination,MMSE)评价两组认知功能,日常生活能力量表(activity of daily living scale,ADL)评价日常生活能力;绘制ROC曲线分析诊断效能。结果AD组患者血清叶酸、VB_(12)、25(OH)D水平和MMSE、ADL评分均明显低于对照组(均P<0.05);3项血清学指标与MMSE、ADL评分呈正相关(均P<0.05)。ROC曲线显示,联合检测叶酸、VB_(12)和25(OH)D诊断AD的AUC值达0.798,敏感性78.3%,特异性72.9%,均明显高于3项指标单独使用时的结果。结论AD患者血清叶酸、VB_(12)、25(OH)D水平显著下降,并与患者的认知功能及日常生活能力衰退程度呈正相关;三者联合检测可提高对AD的诊断效能。展开更多
Self-aligned Titanium Silicide (Salicide), Light-Doped Drain (LDD) technology was studied. Results show that, this technology suppresses effectivily short-channel effects. The sheet resistance of active region decreas...Self-aligned Titanium Silicide (Salicide), Light-Doped Drain (LDD) technology was studied. Results show that, this technology suppresses effectivily short-channel effects. The sheet resistance of active region decreases by four times. The sheet resistance of polysilicon gate region decreases by one order of magnitute. Using this technology, the speed of the 3 μm NMOS 12-bits multiplier increases by two times relative to conventional one.展开更多
Liver cancer,particularly hepatocellular carcinoma,remains a formidable challenge in medical research and requires a deeper understanding of its molecular underpinnings.In a fascinating recent study published in Milit...Liver cancer,particularly hepatocellular carcinoma,remains a formidable challenge in medical research and requires a deeper understanding of its molecular underpinnings.In a fascinating recent study published in Military Medical Research,Xiong et al.[1]revealed the complex roles of F-box and leucine-rich repeat 6(FBXL6)and Kirsten rat sarcoma(KRAS)^(G12D) in the pathogenesis of liver cancer.This research offers critical insights into how these proteins contribute to hepatocellular carcinoma development and progression,potentially paving the way for targeted therapeutic strategies.This commentary analyzes the key findings from this study and their broader implications in oncology.展开更多
目的:观察中药单体姜黄素对ActD/TNF-α协同诱导PC12细胞凋亡的影响,并探讨其机制。方法:采用MTT法确定实验药物的最佳浓度;Hoechst33258荧光染色法观察PC12细胞的凋亡;JC-1荧光分子探针检测线粒体膜电位;Real Time PCR检测凋亡基因Bcl-...目的:观察中药单体姜黄素对ActD/TNF-α协同诱导PC12细胞凋亡的影响,并探讨其机制。方法:采用MTT法确定实验药物的最佳浓度;Hoechst33258荧光染色法观察PC12细胞的凋亡;JC-1荧光分子探针检测线粒体膜电位;Real Time PCR检测凋亡基因Bcl-2/Bax的表达。结果:ActD/TNF-α协同作用可导致PC12细胞的活力降低(P<0.05);出现核固缩、核碎裂现象的细胞增多,细胞凋亡率增高(P<0.05);细胞线粒体膜电位下降;细胞内抗凋亡基因Bcl-2的表达降低(P<0.05)。经姜黄素(5μmol/L)处理后,PC12细胞的活力增强(P<0.05);细胞核固缩、核碎裂现象减少,细胞凋亡率下降(P<0.05);细胞线粒体膜电位上升;细胞内抗凋亡基因Bcl-2的表达增强(P<0.05)。结论:姜黄素可拮抗ActD/TNF-α引起的PC12细胞凋亡,可能与升高线粒体膜电位,促进抗凋亡基因Bcl-2的表达有关。展开更多
文摘目的分析阿尔茨海默病(Alzheimer's disease,AD)患者血清叶酸、维生素B_(12)(VB_(12))、25羟维生素D[25-hydroxyvitamin D,25(OH)D]水平,并评估这3项指标与患者认知功能和日常生活能力的相关性及其诊断AD的效能。方法选取60例AD患者(AD组)和48例体检健康老年人(对照组),检测两组血清叶酸、VB_(12)、25(OH)D水平。运用简易智力状态检查表(mini-mental state examination,MMSE)评价两组认知功能,日常生活能力量表(activity of daily living scale,ADL)评价日常生活能力;绘制ROC曲线分析诊断效能。结果AD组患者血清叶酸、VB_(12)、25(OH)D水平和MMSE、ADL评分均明显低于对照组(均P<0.05);3项血清学指标与MMSE、ADL评分呈正相关(均P<0.05)。ROC曲线显示,联合检测叶酸、VB_(12)和25(OH)D诊断AD的AUC值达0.798,敏感性78.3%,特异性72.9%,均明显高于3项指标单独使用时的结果。结论AD患者血清叶酸、VB_(12)、25(OH)D水平显著下降,并与患者的认知功能及日常生活能力衰退程度呈正相关;三者联合检测可提高对AD的诊断效能。
文摘Self-aligned Titanium Silicide (Salicide), Light-Doped Drain (LDD) technology was studied. Results show that, this technology suppresses effectivily short-channel effects. The sheet resistance of active region decreases by four times. The sheet resistance of polysilicon gate region decreases by one order of magnitute. Using this technology, the speed of the 3 μm NMOS 12-bits multiplier increases by two times relative to conventional one.
基金supported by the National Natural Science Foundation of China(82271518,81971158,and 81671306)the Interdisciplinary Innovative Talents Foundation from Renmin Hospital of Wuhan University(JCRCFZ-2022-030)the Guiding Projects of Traditional Chinese Medicine in 2023–2024 by Hubei Provincial Administration of Traditional Chinese Medicine(ZY2023F038).
文摘Liver cancer,particularly hepatocellular carcinoma,remains a formidable challenge in medical research and requires a deeper understanding of its molecular underpinnings.In a fascinating recent study published in Military Medical Research,Xiong et al.[1]revealed the complex roles of F-box and leucine-rich repeat 6(FBXL6)and Kirsten rat sarcoma(KRAS)^(G12D) in the pathogenesis of liver cancer.This research offers critical insights into how these proteins contribute to hepatocellular carcinoma development and progression,potentially paving the way for targeted therapeutic strategies.This commentary analyzes the key findings from this study and their broader implications in oncology.
文摘目的:观察中药单体姜黄素对ActD/TNF-α协同诱导PC12细胞凋亡的影响,并探讨其机制。方法:采用MTT法确定实验药物的最佳浓度;Hoechst33258荧光染色法观察PC12细胞的凋亡;JC-1荧光分子探针检测线粒体膜电位;Real Time PCR检测凋亡基因Bcl-2/Bax的表达。结果:ActD/TNF-α协同作用可导致PC12细胞的活力降低(P<0.05);出现核固缩、核碎裂现象的细胞增多,细胞凋亡率增高(P<0.05);细胞线粒体膜电位下降;细胞内抗凋亡基因Bcl-2的表达降低(P<0.05)。经姜黄素(5μmol/L)处理后,PC12细胞的活力增强(P<0.05);细胞核固缩、核碎裂现象减少,细胞凋亡率下降(P<0.05);细胞线粒体膜电位上升;细胞内抗凋亡基因Bcl-2的表达增强(P<0.05)。结论:姜黄素可拮抗ActD/TNF-α引起的PC12细胞凋亡,可能与升高线粒体膜电位,促进抗凋亡基因Bcl-2的表达有关。
