目的分析阿尔茨海默病(Alzheimer's disease,AD)患者血清叶酸、维生素B_(12)(VB_(12))、25羟维生素D[25-hydroxyvitamin D,25(OH)D]水平,并评估这3项指标与患者认知功能和日常生活能力的相关性及其诊断AD的效能。方法选取60例AD患者...目的分析阿尔茨海默病(Alzheimer's disease,AD)患者血清叶酸、维生素B_(12)(VB_(12))、25羟维生素D[25-hydroxyvitamin D,25(OH)D]水平,并评估这3项指标与患者认知功能和日常生活能力的相关性及其诊断AD的效能。方法选取60例AD患者(AD组)和48例体检健康老年人(对照组),检测两组血清叶酸、VB_(12)、25(OH)D水平。运用简易智力状态检查表(mini-mental state examination,MMSE)评价两组认知功能,日常生活能力量表(activity of daily living scale,ADL)评价日常生活能力;绘制ROC曲线分析诊断效能。结果AD组患者血清叶酸、VB_(12)、25(OH)D水平和MMSE、ADL评分均明显低于对照组(均P<0.05);3项血清学指标与MMSE、ADL评分呈正相关(均P<0.05)。ROC曲线显示,联合检测叶酸、VB_(12)和25(OH)D诊断AD的AUC值达0.798,敏感性78.3%,特异性72.9%,均明显高于3项指标单独使用时的结果。结论AD患者血清叶酸、VB_(12)、25(OH)D水平显著下降,并与患者的认知功能及日常生活能力衰退程度呈正相关;三者联合检测可提高对AD的诊断效能。展开更多
Liver cancer,particularly hepatocellular carcinoma,remains a formidable challenge in medical research and requires a deeper understanding of its molecular underpinnings.In a fascinating recent study published in Milit...Liver cancer,particularly hepatocellular carcinoma,remains a formidable challenge in medical research and requires a deeper understanding of its molecular underpinnings.In a fascinating recent study published in Military Medical Research,Xiong et al.[1]revealed the complex roles of F-box and leucine-rich repeat 6(FBXL6)and Kirsten rat sarcoma(KRAS)^(G12D) in the pathogenesis of liver cancer.This research offers critical insights into how these proteins contribute to hepatocellular carcinoma development and progression,potentially paving the way for targeted therapeutic strategies.This commentary analyzes the key findings from this study and their broader implications in oncology.展开更多
This work proposed a strategy of indirectly inducing uniform microarc discharge by controlling the content and distribution ofβ-Mg_(17)Al_(12)phase in AZ91D Mg alloy.Two kinds of nano-particles(ZrO_(2)and TiO_(2))wer...This work proposed a strategy of indirectly inducing uniform microarc discharge by controlling the content and distribution ofβ-Mg_(17)Al_(12)phase in AZ91D Mg alloy.Two kinds of nano-particles(ZrO_(2)and TiO_(2))were designed to be added into the substrate of Mg alloy by friction stir processing(FSP).Then,Mg alloy sample designed with different precipitated morphology ofβ-Mg_(17)Al_(12)phase was treated by microarc oxidation(MAO)in Na_(3)PO_(4)/Na2SiO3electrolyte.The characteristics and performance of the MAO coating was analyzed using scanning electron microscopy(SEM),energy dispersive spectrometer(EDS),X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),contact angle meter,and potentiodynamic polarization.It was found that the coarseα-Mg grains in extruded AZ91D Mg alloy were refined by FSP,and theβ-Mg_(17)Al_(12)phase with reticular structure was broken and dispersed.The nano-ZrO_(2)particles were pinned at the grain boundary by FSP,which refined theα-Mg grain and promoted the precipitation ofβ-Mg_(17)Al_(12)phase in grains.It effectively inhibited the“cascade”phenomenon of microarcs,which induced the uniform distribution of discharge pores.The MAO coating on Zr-FSP sample had good wettability and corrosion resistance.However,TiO_(2)particles were hardly detected in the coating on TiFSP sample.展开更多
Objectives:The Kirsten rat sarcoma virus(KRAS)G12D oncogenic mutation poses a significant challenge in treating solid tumors due to the lack of specific and effective therapeutic interventions.This study aims to explore...Objectives:The Kirsten rat sarcoma virus(KRAS)G12D oncogenic mutation poses a significant challenge in treating solid tumors due to the lack of specific and effective therapeutic interventions.This study aims to explore innovative approaches in T cell receptor(TCR)engineering and characterization to target the KRAS G12D7-16 mutation,providing potential strategies for overcoming this therapeutic challenge.Methods:In this innovative study,we engineered and characterized two T cell receptors(TCRs),KDA11-01 and KDA11-02 with high affinity for the KRAS G12D7-16 mutation.These TCRs were isolated from tumor-infiltrating lymphocytes(TILs)derived from tumor tissues of patients with the KRAS G12D mutation.We assessed their specificity and anti-tumor activity in vitro using various cancer cell lines.Results:KDA11-01 and KDA11-02 demonstrated exceptional specificity for the HLA-A*11:01-restricted KRAS G12D7-16 epitope,significantly inducing IFN-γrelease and eliminating tumor cells without cross-reactivity or alloreactivity.Conclusions:The successful development of KDA11-01 and KDA11-02 introduces a novel and precise TCR-based therapeutic strategy against KRAS G12D mutation,showing potential for significant advancements in cancer immunotherapy.展开更多
文摘目的分析阿尔茨海默病(Alzheimer's disease,AD)患者血清叶酸、维生素B_(12)(VB_(12))、25羟维生素D[25-hydroxyvitamin D,25(OH)D]水平,并评估这3项指标与患者认知功能和日常生活能力的相关性及其诊断AD的效能。方法选取60例AD患者(AD组)和48例体检健康老年人(对照组),检测两组血清叶酸、VB_(12)、25(OH)D水平。运用简易智力状态检查表(mini-mental state examination,MMSE)评价两组认知功能,日常生活能力量表(activity of daily living scale,ADL)评价日常生活能力;绘制ROC曲线分析诊断效能。结果AD组患者血清叶酸、VB_(12)、25(OH)D水平和MMSE、ADL评分均明显低于对照组(均P<0.05);3项血清学指标与MMSE、ADL评分呈正相关(均P<0.05)。ROC曲线显示,联合检测叶酸、VB_(12)和25(OH)D诊断AD的AUC值达0.798,敏感性78.3%,特异性72.9%,均明显高于3项指标单独使用时的结果。结论AD患者血清叶酸、VB_(12)、25(OH)D水平显著下降,并与患者的认知功能及日常生活能力衰退程度呈正相关;三者联合检测可提高对AD的诊断效能。
基金supported by the National Natural Science Foundation of China(82271518,81971158,and 81671306)the Interdisciplinary Innovative Talents Foundation from Renmin Hospital of Wuhan University(JCRCFZ-2022-030)the Guiding Projects of Traditional Chinese Medicine in 2023–2024 by Hubei Provincial Administration of Traditional Chinese Medicine(ZY2023F038).
文摘Liver cancer,particularly hepatocellular carcinoma,remains a formidable challenge in medical research and requires a deeper understanding of its molecular underpinnings.In a fascinating recent study published in Military Medical Research,Xiong et al.[1]revealed the complex roles of F-box and leucine-rich repeat 6(FBXL6)and Kirsten rat sarcoma(KRAS)^(G12D) in the pathogenesis of liver cancer.This research offers critical insights into how these proteins contribute to hepatocellular carcinoma development and progression,potentially paving the way for targeted therapeutic strategies.This commentary analyzes the key findings from this study and their broader implications in oncology.
基金funded by China Postdoctoral Science Foundation(No.2021M700569)Chongqing Postdoctoral Science Foundation(No.7 cstc2021jcyj-bshX0087)。
文摘This work proposed a strategy of indirectly inducing uniform microarc discharge by controlling the content and distribution ofβ-Mg_(17)Al_(12)phase in AZ91D Mg alloy.Two kinds of nano-particles(ZrO_(2)and TiO_(2))were designed to be added into the substrate of Mg alloy by friction stir processing(FSP).Then,Mg alloy sample designed with different precipitated morphology ofβ-Mg_(17)Al_(12)phase was treated by microarc oxidation(MAO)in Na_(3)PO_(4)/Na2SiO3electrolyte.The characteristics and performance of the MAO coating was analyzed using scanning electron microscopy(SEM),energy dispersive spectrometer(EDS),X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),contact angle meter,and potentiodynamic polarization.It was found that the coarseα-Mg grains in extruded AZ91D Mg alloy were refined by FSP,and theβ-Mg_(17)Al_(12)phase with reticular structure was broken and dispersed.The nano-ZrO_(2)particles were pinned at the grain boundary by FSP,which refined theα-Mg grain and promoted the precipitation ofβ-Mg_(17)Al_(12)phase in grains.It effectively inhibited the“cascade”phenomenon of microarcs,which induced the uniform distribution of discharge pores.The MAO coating on Zr-FSP sample had good wettability and corrosion resistance.However,TiO_(2)particles were hardly detected in the coating on TiFSP sample.
基金funded by the key R&D Project of Hubei Province(Social Development),China(2022BCA018)the Cooperative Innovation Center of Industrial Fermentation(Ministry of Education&Hubei Province),China(2022KF16)to Kanghong Hu.
文摘Objectives:The Kirsten rat sarcoma virus(KRAS)G12D oncogenic mutation poses a significant challenge in treating solid tumors due to the lack of specific and effective therapeutic interventions.This study aims to explore innovative approaches in T cell receptor(TCR)engineering and characterization to target the KRAS G12D7-16 mutation,providing potential strategies for overcoming this therapeutic challenge.Methods:In this innovative study,we engineered and characterized two T cell receptors(TCRs),KDA11-01 and KDA11-02 with high affinity for the KRAS G12D7-16 mutation.These TCRs were isolated from tumor-infiltrating lymphocytes(TILs)derived from tumor tissues of patients with the KRAS G12D mutation.We assessed their specificity and anti-tumor activity in vitro using various cancer cell lines.Results:KDA11-01 and KDA11-02 demonstrated exceptional specificity for the HLA-A*11:01-restricted KRAS G12D7-16 epitope,significantly inducing IFN-γrelease and eliminating tumor cells without cross-reactivity or alloreactivity.Conclusions:The successful development of KDA11-01 and KDA11-02 introduces a novel and precise TCR-based therapeutic strategy against KRAS G12D mutation,showing potential for significant advancements in cancer immunotherapy.
