We reported a new protocol for constructing a unique indolizine-containing perifused tricycle by combining a copper relay-catalyzed three-component cascade with a 12πelectrocyclization reaction from readily available...We reported a new protocol for constructing a unique indolizine-containing perifused tricycle by combining a copper relay-catalyzed three-component cascade with a 12πelectrocyclization reaction from readily available starting materials.The indolizine generated by the copper-catalyzed multicomponent cascade is a key intermediate obtained during subsequent spontaneous electrocyclization.In contrast to the traditional SEAr reaction model,this study is based on the tetraene-like character of indolizine to form an aromatic heterocycle 12πelectron system that can spontaneously trigger 12πelectrocyclization.This transformation can not only enhance the reactivity of indolizine but also improve the electrocyclization model.This convergent strategy can significantly enhance synthetic versatility,and the resulting perifused cycle products containing indolizine may serve as a molecular platform for the discovery and characterization of pharmaceuticals and functional molecules.展开更多
目的探讨联合检测同型半胱氨酸(homocysteine,Hcy)与血管活性物质12(Apelin-12)在冠心病诊断中的协同作用,为临床提供更优化的无创筛查方案。方法选取2022年7月至2023年6月医院经冠脉造影确诊的冠心病患者52例(观察组)及健康体检者52例...目的探讨联合检测同型半胱氨酸(homocysteine,Hcy)与血管活性物质12(Apelin-12)在冠心病诊断中的协同作用,为临床提供更优化的无创筛查方案。方法选取2022年7月至2023年6月医院经冠脉造影确诊的冠心病患者52例(观察组)及健康体检者52例(对照组),采集两组受试者静脉血,检测两组血清Hcy(速率法)及Apelin-12(ELISA法)水平,分析Hcy、Apelin-12单独或联合检测的诊断效能(灵敏度、特异性、准确度及ROC曲线下面积),并分析Hcy和Apelin-12的相关性。结果观察组Hcy水平显著高于对照组(18.45±1.57μmol/L vs 11.63±1.24μmol/L,P<0.001),观察组Apelin-12水平显著低于对照组(0.94±0.15μg/L vs 2.31±0.26μg/L,P<0.001),Hcy和Apelin-12呈负相关(r=-0.732,P<0.001)。Hcy与Apelin-12联合检测的灵敏度(94.23%)、特异性(92.31%)及准确度(93.27%)均显著优于单一检测(P<0.05),联合检测ROC曲线下面积(AUC=0.96)高于Hcy(AUC=0.82)和Apelin-12(AUC=0.84)。结论Hcy与Apelin-12呈负相关,联合检测在冠心病人群中具有较高的检测效能,这或许能作为筛查冠心病患者的潜在生物学标志物的补充。展开更多
基金The authors thank NSFC(grant nos.21772019 and 22371024)support from the Venture&Innovation Support Program for Chongqing Overseas Returnees(grant nos.cx2019007 and cx2020047)the Basic and Frontier Research Project of Chongqing(grant no.CSTB2022NSCQ-MSX0320).
文摘We reported a new protocol for constructing a unique indolizine-containing perifused tricycle by combining a copper relay-catalyzed three-component cascade with a 12πelectrocyclization reaction from readily available starting materials.The indolizine generated by the copper-catalyzed multicomponent cascade is a key intermediate obtained during subsequent spontaneous electrocyclization.In contrast to the traditional SEAr reaction model,this study is based on the tetraene-like character of indolizine to form an aromatic heterocycle 12πelectron system that can spontaneously trigger 12πelectrocyclization.This transformation can not only enhance the reactivity of indolizine but also improve the electrocyclization model.This convergent strategy can significantly enhance synthetic versatility,and the resulting perifused cycle products containing indolizine may serve as a molecular platform for the discovery and characterization of pharmaceuticals and functional molecules.
文摘目的探讨联合检测同型半胱氨酸(homocysteine,Hcy)与血管活性物质12(Apelin-12)在冠心病诊断中的协同作用,为临床提供更优化的无创筛查方案。方法选取2022年7月至2023年6月医院经冠脉造影确诊的冠心病患者52例(观察组)及健康体检者52例(对照组),采集两组受试者静脉血,检测两组血清Hcy(速率法)及Apelin-12(ELISA法)水平,分析Hcy、Apelin-12单独或联合检测的诊断效能(灵敏度、特异性、准确度及ROC曲线下面积),并分析Hcy和Apelin-12的相关性。结果观察组Hcy水平显著高于对照组(18.45±1.57μmol/L vs 11.63±1.24μmol/L,P<0.001),观察组Apelin-12水平显著低于对照组(0.94±0.15μg/L vs 2.31±0.26μg/L,P<0.001),Hcy和Apelin-12呈负相关(r=-0.732,P<0.001)。Hcy与Apelin-12联合检测的灵敏度(94.23%)、特异性(92.31%)及准确度(93.27%)均显著优于单一检测(P<0.05),联合检测ROC曲线下面积(AUC=0.96)高于Hcy(AUC=0.82)和Apelin-12(AUC=0.84)。结论Hcy与Apelin-12呈负相关,联合检测在冠心病人群中具有较高的检测效能,这或许能作为筛查冠心病患者的潜在生物学标志物的补充。
