By means of laser shock processing(LSP)treatment,a nanostructured surface layer was formed on 1Cr11Ni2W2MoV stainless steel.Group contrastive high cycle fatigue tests were carried out in 1Cr11Ni2W2MoV stainless steel ...By means of laser shock processing(LSP)treatment,a nanostructured surface layer was formed on 1Cr11Ni2W2MoV stainless steel.Group contrastive high cycle fatigue tests were carried out in 1Cr11Ni2W2MoV stainless steel before and after LSP treated.The experimental data was analyzed by mathematical statistics method and the influence mechanism of fatigue properties affected by LSP treatment was also researched.The results indicate that LSP treatment can improve the high cycle fatigue life of 1Cr11Ni2W2MoV stainless steel effectively.The ultra-fine grained surface layer with residual compressive stress and nanostructured surface layer make great contributions to the improvement in fatigue properties of 1Cr11Ni2W2MoV stainless steel.展开更多
Objective: To determine the effect of cis-9, trans-1 1-conjugated linoleic acid on the cell cycle of mammary cancer cells (MCF-7) and the possible mechanism of the inhibitory effect of c9,t11-CLA. Methods: Using cell ...Objective: To determine the effect of cis-9, trans-1 1-conjugated linoleic acid on the cell cycle of mammary cancer cells (MCF-7) and the possible mechanism of the inhibitory effect of c9,t11-CLA. Methods: Using cell culture and immunocytochemical techniques, we examined the cell growth, DNA synthesis, expression of PCNA, cyclin A, B1, D1, p16ink4a and p21cip/waf1 of MCF-7 cells at various c9,t11-CLA concentrations (25μM, 50μM, 100μM and 200μM), at 24h and 48h. 96% ethand was used as negative control. Results: The cell growth and DNA synthesis of MCF-7 cells were inhibited by c9,t11-CLA. After treatment with various doses of c9,t11-CLA mentioned above for 8 days, the inhibition frequency was 27.18%, 35.43%, 91.05%, and 92.86%, respectively. Inhibitory effect of c9,t11-CLA on DNA synthesis (except for 25μM, 24h) was demonstrated by significantly less incorporation of 3H-TdR than the negative control (P<0.05 and P<0.01). To further investigate the influence of the cell cycle progression, we found that c9,t11-CLA may arrest the cell cycle of MCF-7 cells. Immunocytochemical staining demonstrated that incubation with different concentration of c9,t11-CLA at various times significantly decreased the expression of PCNA, Cyclin A, B1, D1 in MCF-7 cells compared to the negative control (P<0.01), whereas the expression of p16ink4a and p21cip/waf1, cyclin-dependent kinases inhibitors (CDKI), were increased. Conclusions: The cell growth and proliferation of MCF-7 cells is inhibited by c9,t11-CLA via blocking cell cycle, accompanying reduced expression of cyclin A, B1, D1 and enhanced expression of CDKI (p16ink4a and p21cip/waf1).展开更多
For the 22-year solar cycle oscillation there is no external time dependent source. A nonlinear oscillation, the solar cycle must be generated internally, and Babcock-Leighton models apply an artificial nonlinear sour...For the 22-year solar cycle oscillation there is no external time dependent source. A nonlinear oscillation, the solar cycle must be generated internally, and Babcock-Leighton models apply an artificial nonlinear source term that can simulate the observations—which leaves open the question of the actual source mechanism for the solar cycle. Addressing this question, we propose to take guidance from the wave mechanism that generates the 2-year Quasi-biennial Oscillation (QBO) in the Earth atmosphere. Upward propagating gravity waves, eastward and westward, deposit momentum to generate the observed zonal wind oscillation. On the Sun, helioseismology has provided a thorough understanding of the acoustic p-waves, which propagate down into the convective envelope guided by the increasing temperature and related propagation velocity. Near the tachocline with low turbulent viscosity, the waves propagating eastward and westward can produce an axisymmetric 22-year oscillation of the zonal flow velocities that can generate the magnetic solar dynamo. Following the Earth model, waves in opposite directions can generate in the Sun wind and magnetic field oscillations in opposite directions, the proposition of a potential solar cycle mechanism.展开更多
The X ray (1-8?) flux data of the solar flares are analyzed. The data used were obtained on GOES satellite during the solar cycles 21 and 22. The total number of the flares is more than 33000. The flare distributions ...The X ray (1-8?) flux data of the solar flares are analyzed. The data used were obtained on GOES satellite during the solar cycles 21 and 22. The total number of the flares is more than 33000. The flare distributions according to their X ray classes, durations and intensities are constructed. The evolution of these distributions in solar cycle as well as from cycle to cycle is studied. The statistical analysis of the flare characteristics is made. There was confirmed the result of more frequent occurrence for powerful flares in cycle 21 decline phase.展开更多
Thermal cycling procedure during laser additive manufacturing (LAM) process causes the appearance of bright and dark patterns on the etched surface of TC11 alloy components. The formation mechanisms of these patterns ...Thermal cycling procedure during laser additive manufacturing (LAM) process causes the appearance of bright and dark patterns on the etched surface of TC11 alloy components. The formation mechanisms of these patterns and the solid-state transformation related to LAM process are systematically investigated with the predication of temperature fields using the finite element software ABAQUS. The results indicate that by increasing subsequent thermal cycles, the peak temperatures for every cycle decrease. When peak temperatures are above Tβ(phase transition temperature of β phase), which is 1010℃ in TC11 alloy, no pattern is observed. Meanwhile, a decrease in peak temperature leads to appearance of an ultrafine basket-weave α+β microstructure (dark contrast) with gradually increased amount of α colonies in the alloy. A special bimodal microstructure with ‘fork-like'α lamella appears in the layer when the peak temperatures of thermal cycles firstly fall into α+β dual-phase region. And this special bimodal microstructure gives a bright contrast and only appears at the region where the peak temperatures are below 970℃, leaving the rest region with a dark contrast. With the continuous increase in thermal cycles in α+β dual-phase region,α lamella gradually coarsens. After five thermal cycles in α+β two-phase region, no further changes in microstructure are observed, and the morphologies of α lamella in dark and bright regions are almost the same but with different amounts of α phase.展开更多
The PRR11 gene(Proline Rich 11)has been implicated in lung cancer;however,relationship between PRR11 and immune infiltration is not clearly understood.In this study,we used The Cancer Genome Atlas(TCGA)data to analyze...The PRR11 gene(Proline Rich 11)has been implicated in lung cancer;however,relationship between PRR11 and immune infiltration is not clearly understood.In this study,we used The Cancer Genome Atlas(TCGA)data to analyze the lung adenocarcinoma patients;PRR11 gene expression,clinicopathological findings,enrichment,and immune infiltration were also studied.PRR11immune response expression assays in lung adenocarcinoma(LUAD)were performed using TIMER,and statistical analysis and visualization were conducted using R software.All data were verified using Gene Expression Profiling Interactive Analysis(GEPIA),and the Human Protein Atlas(HPA).We found that PRR11 was an important prognostic factor in patients with LUAD.PRR11 expression was correlated with tumor stage and progression.Gene Set Enrichment Analysis(GSEA)showed that PRR11was enriched in the cell cycle regulatory pathways.Immune infiltration analysis revealed that the number of T helper 2(Th2)cells increased when PRR11 was overexpressed.These results confirm the role of PRR11 as a prognostic marker of lung adenocarcinoma by controlling the cell cycle and influencing the immune system to facilitate lung cancer progression.展开更多
文摘By means of laser shock processing(LSP)treatment,a nanostructured surface layer was formed on 1Cr11Ni2W2MoV stainless steel.Group contrastive high cycle fatigue tests were carried out in 1Cr11Ni2W2MoV stainless steel before and after LSP treated.The experimental data was analyzed by mathematical statistics method and the influence mechanism of fatigue properties affected by LSP treatment was also researched.The results indicate that LSP treatment can improve the high cycle fatigue life of 1Cr11Ni2W2MoV stainless steel effectively.The ultra-fine grained surface layer with residual compressive stress and nanostructured surface layer make great contributions to the improvement in fatigue properties of 1Cr11Ni2W2MoV stainless steel.
基金This work was supported by the National Natural Science Foundation of China(No.39870661). Phone: (0086-451)-3641309 Fax: (0086-451)-3641253
文摘Objective: To determine the effect of cis-9, trans-1 1-conjugated linoleic acid on the cell cycle of mammary cancer cells (MCF-7) and the possible mechanism of the inhibitory effect of c9,t11-CLA. Methods: Using cell culture and immunocytochemical techniques, we examined the cell growth, DNA synthesis, expression of PCNA, cyclin A, B1, D1, p16ink4a and p21cip/waf1 of MCF-7 cells at various c9,t11-CLA concentrations (25μM, 50μM, 100μM and 200μM), at 24h and 48h. 96% ethand was used as negative control. Results: The cell growth and DNA synthesis of MCF-7 cells were inhibited by c9,t11-CLA. After treatment with various doses of c9,t11-CLA mentioned above for 8 days, the inhibition frequency was 27.18%, 35.43%, 91.05%, and 92.86%, respectively. Inhibitory effect of c9,t11-CLA on DNA synthesis (except for 25μM, 24h) was demonstrated by significantly less incorporation of 3H-TdR than the negative control (P<0.05 and P<0.01). To further investigate the influence of the cell cycle progression, we found that c9,t11-CLA may arrest the cell cycle of MCF-7 cells. Immunocytochemical staining demonstrated that incubation with different concentration of c9,t11-CLA at various times significantly decreased the expression of PCNA, Cyclin A, B1, D1 in MCF-7 cells compared to the negative control (P<0.01), whereas the expression of p16ink4a and p21cip/waf1, cyclin-dependent kinases inhibitors (CDKI), were increased. Conclusions: The cell growth and proliferation of MCF-7 cells is inhibited by c9,t11-CLA via blocking cell cycle, accompanying reduced expression of cyclin A, B1, D1 and enhanced expression of CDKI (p16ink4a and p21cip/waf1).
文摘For the 22-year solar cycle oscillation there is no external time dependent source. A nonlinear oscillation, the solar cycle must be generated internally, and Babcock-Leighton models apply an artificial nonlinear source term that can simulate the observations—which leaves open the question of the actual source mechanism for the solar cycle. Addressing this question, we propose to take guidance from the wave mechanism that generates the 2-year Quasi-biennial Oscillation (QBO) in the Earth atmosphere. Upward propagating gravity waves, eastward and westward, deposit momentum to generate the observed zonal wind oscillation. On the Sun, helioseismology has provided a thorough understanding of the acoustic p-waves, which propagate down into the convective envelope guided by the increasing temperature and related propagation velocity. Near the tachocline with low turbulent viscosity, the waves propagating eastward and westward can produce an axisymmetric 22-year oscillation of the zonal flow velocities that can generate the magnetic solar dynamo. Following the Earth model, waves in opposite directions can generate in the Sun wind and magnetic field oscillations in opposite directions, the proposition of a potential solar cycle mechanism.
文摘The X ray (1-8?) flux data of the solar flares are analyzed. The data used were obtained on GOES satellite during the solar cycles 21 and 22. The total number of the flares is more than 33000. The flare distributions according to their X ray classes, durations and intensities are constructed. The evolution of these distributions in solar cycle as well as from cycle to cycle is studied. The statistical analysis of the flare characteristics is made. There was confirmed the result of more frequent occurrence for powerful flares in cycle 21 decline phase.
文摘Thermal cycling procedure during laser additive manufacturing (LAM) process causes the appearance of bright and dark patterns on the etched surface of TC11 alloy components. The formation mechanisms of these patterns and the solid-state transformation related to LAM process are systematically investigated with the predication of temperature fields using the finite element software ABAQUS. The results indicate that by increasing subsequent thermal cycles, the peak temperatures for every cycle decrease. When peak temperatures are above Tβ(phase transition temperature of β phase), which is 1010℃ in TC11 alloy, no pattern is observed. Meanwhile, a decrease in peak temperature leads to appearance of an ultrafine basket-weave α+β microstructure (dark contrast) with gradually increased amount of α colonies in the alloy. A special bimodal microstructure with ‘fork-like'α lamella appears in the layer when the peak temperatures of thermal cycles firstly fall into α+β dual-phase region. And this special bimodal microstructure gives a bright contrast and only appears at the region where the peak temperatures are below 970℃, leaving the rest region with a dark contrast. With the continuous increase in thermal cycles in α+β dual-phase region,α lamella gradually coarsens. After five thermal cycles in α+β two-phase region, no further changes in microstructure are observed, and the morphologies of α lamella in dark and bright regions are almost the same but with different amounts of α phase.
基金supported by the Traditional Chinese Medicine Science and Technology Project of Shandong Province [grant number 2020Q139]。
文摘The PRR11 gene(Proline Rich 11)has been implicated in lung cancer;however,relationship between PRR11 and immune infiltration is not clearly understood.In this study,we used The Cancer Genome Atlas(TCGA)data to analyze the lung adenocarcinoma patients;PRR11 gene expression,clinicopathological findings,enrichment,and immune infiltration were also studied.PRR11immune response expression assays in lung adenocarcinoma(LUAD)were performed using TIMER,and statistical analysis and visualization were conducted using R software.All data were verified using Gene Expression Profiling Interactive Analysis(GEPIA),and the Human Protein Atlas(HPA).We found that PRR11 was an important prognostic factor in patients with LUAD.PRR11 expression was correlated with tumor stage and progression.Gene Set Enrichment Analysis(GSEA)showed that PRR11was enriched in the cell cycle regulatory pathways.Immune infiltration analysis revealed that the number of T helper 2(Th2)cells increased when PRR11 was overexpressed.These results confirm the role of PRR11 as a prognostic marker of lung adenocarcinoma by controlling the cell cycle and influencing the immune system to facilitate lung cancer progression.
