In this paper we discuss the K-groups of Wiener algebra ;W. For the 1-shift space XGM2,We obtain a characterization of Fredholm operators on X^nGM2 for all n ∈ N. We also calculate the K-groups of operator algebra on...In this paper we discuss the K-groups of Wiener algebra ;W. For the 1-shift space XGM2,We obtain a characterization of Fredholm operators on X^nGM2 for all n ∈ N. We also calculate the K-groups of operator algebra on the 1-shift space XGM2.展开更多
目的:探讨翼螺旋转录因子FOXO1的DNA结合域(FOXO1 DNA binding domain,FOXO1-DBD)的表达、纯化及与DNA的结合特性。方法:采用优化FOXO1-DNA的基因序列和低温诱导的方式实现FOXO1-DBD蛋白的可溶性表达,通过镍亲和层析及阳离子交换层析进...目的:探讨翼螺旋转录因子FOXO1的DNA结合域(FOXO1 DNA binding domain,FOXO1-DBD)的表达、纯化及与DNA的结合特性。方法:采用优化FOXO1-DNA的基因序列和低温诱导的方式实现FOXO1-DBD蛋白的可溶性表达,通过镍亲和层析及阳离子交换层析进行纯化,并经凝胶迁移实验(electrophoretic mobility shift assay,EMSA)验证FOXO1-DBD的DNA结合特性。结果:优化后的FOXO1基因在21℃时编码的蛋白大多以可溶性方式表达,通过两步纯化即可获得95%以上纯度的FOXO1-DBD蛋白,纯化的蛋白与含FOX家族DNA结合基序(G/ATAAACA)的DNA序列显示良好的结合特性。结论:建立了FOXO1-DBD蛋白高效表达、纯化的方法,验证了FOXO1蛋白在识别DNA上的复杂性。展开更多
应用移动1/N展开方法,计算Van del pol振子方程的本征值。对v=0,且应用SUSY—QM计算其本征值。当光泵参数g远低于阈值或在阈值附近,所有本征值都能和数值计算结果相吻合。当g远高于阈值时,基态和第一激发态本征值亦能和数值计算结果相...应用移动1/N展开方法,计算Van del pol振子方程的本征值。对v=0,且应用SUSY—QM计算其本征值。当光泵参数g远低于阈值或在阈值附近,所有本征值都能和数值计算结果相吻合。当g远高于阈值时,基态和第一激发态本征值亦能和数值计算结果相吻合。展开更多
基金National Natural Science Foundation of China (10471025,10771034)National Natural Science Foundation of Fujian Province (S0650009)Foudation of the Education Department of Fujian Provience (JA04170,JB07047)
文摘In this paper we discuss the K-groups of Wiener algebra ;W. For the 1-shift space XGM2,We obtain a characterization of Fredholm operators on X^nGM2 for all n ∈ N. We also calculate the K-groups of operator algebra on the 1-shift space XGM2.
