Krill oil(KO)exhibits various biological activities,such as anti-inflammatory and antitumor effects.However,the inhibitory effects of benign prostatic hyperplasia(BPH)in vitro and in vivo have not yet been studied.Thi...Krill oil(KO)exhibits various biological activities,such as anti-inflammatory and antitumor effects.However,the inhibitory effects of benign prostatic hyperplasia(BPH)in vitro and in vivo have not yet been studied.This study investigated the anti-BPH effects of KO extracted by an enzymatic hydrolysis method.KO treatment inhibited the proliferation of WMPY-1 and BPH-1 cells by induction of G0/G1 phase arrest through the modulation of positive and negative regulators in both prostate cell types.KO treatment stimulated phosphorylation of c-Jun N-terminal kinase(JNK)and p38 signaling.In addition,KO changed the expression of BPH-related markers(5α-reductase,androgen receptor,FGF,Bcl-2,and Bax)and the activity of the proliferation-mediated NF-κB binding motif.KO-induced levels of proliferation-mediated molecules of prostate cells were attenuated in the presence of siRNA-specific p-38(si-p38)and JNK(si-JNK).Furthermore,the administration of KO alleviated prostate size and weight and the cell layer thickness of prostate glands in a testosterone enanthate-induced BPH rat model.KO treatment altered the level of dihydrotestosterone in serum and the expression levels of BPH-related markers in prostate tissues.Finally,KO-mediated inhibition of prostatic growth was validated by histological analysis.These results suggest that KO has an inhibitory effect on BPH in prostate cells in vitro and in vivo.Thus,KO might be a potential prophylactic or therapeutic agent for patients with BPH.展开更多
Reaction of polymer-supported a-selenoaldehydes with Grignard reagents afforded polymer-bound B-hydroxyalkyl selenides, which treated with thionyl chloride and triethylamine leading to (E)-1, 2-disubstituted ethenes i...Reaction of polymer-supported a-selenoaldehydes with Grignard reagents afforded polymer-bound B-hydroxyalkyl selenides, which treated with thionyl chloride and triethylamine leading to (E)-1, 2-disubstituted ethenes in good yield.展开更多
环己酮肟气相Beckmann重排反应RBS-1催化剂在高温反应时易失活,导致催化剂费用居高不下。为提高技术经济性,实现失活催化剂性能的高效恢复,系统研究RBS-1催化剂失活机理、再生手段及催化性能。采用N_(2)物理吸附-脱附技术考察催化剂失...环己酮肟气相Beckmann重排反应RBS-1催化剂在高温反应时易失活,导致催化剂费用居高不下。为提高技术经济性,实现失活催化剂性能的高效恢复,系统研究RBS-1催化剂失活机理、再生手段及催化性能。采用N_(2)物理吸附-脱附技术考察催化剂失活前后与再生前后的比表面积及孔结构变化,并结合XRD、FT-IR和^(29)Si MAS NMR表征技术探讨了RBS-1催化剂在失活与再生过程中活性中心的演变规律。研究发现,失活RBS-1催化剂物相结构保持完好,但BET比表面积和孔体积均有明显降低,说明催化剂失活主要为积炭覆盖催化剂活性位点并堵塞孔道所致。针对积炭堵孔导致的催化剂失活问题,采用烧炭、含氮化合物改性处理构建巢式硅羟基活性位点等手段对失活催化剂进行再生研究。结果表明,在530℃下烧炭能将催化剂表面及孔道内的大部分积炭烧除,催化剂性能可恢复90%以上;对脱炭后的催化剂进一步改性处理,最优催化剂性能可恢复98%以上。展开更多
基金supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(2018R1A6A1A03025159).
文摘Krill oil(KO)exhibits various biological activities,such as anti-inflammatory and antitumor effects.However,the inhibitory effects of benign prostatic hyperplasia(BPH)in vitro and in vivo have not yet been studied.This study investigated the anti-BPH effects of KO extracted by an enzymatic hydrolysis method.KO treatment inhibited the proliferation of WMPY-1 and BPH-1 cells by induction of G0/G1 phase arrest through the modulation of positive and negative regulators in both prostate cell types.KO treatment stimulated phosphorylation of c-Jun N-terminal kinase(JNK)and p38 signaling.In addition,KO changed the expression of BPH-related markers(5α-reductase,androgen receptor,FGF,Bcl-2,and Bax)and the activity of the proliferation-mediated NF-κB binding motif.KO-induced levels of proliferation-mediated molecules of prostate cells were attenuated in the presence of siRNA-specific p-38(si-p38)and JNK(si-JNK).Furthermore,the administration of KO alleviated prostate size and weight and the cell layer thickness of prostate glands in a testosterone enanthate-induced BPH rat model.KO treatment altered the level of dihydrotestosterone in serum and the expression levels of BPH-related markers in prostate tissues.Finally,KO-mediated inhibition of prostatic growth was validated by histological analysis.These results suggest that KO has an inhibitory effect on BPH in prostate cells in vitro and in vivo.Thus,KO might be a potential prophylactic or therapeutic agent for patients with BPH.
基金Project 29932020 was supported by the National Natural Science Foundation of China.
文摘Reaction of polymer-supported a-selenoaldehydes with Grignard reagents afforded polymer-bound B-hydroxyalkyl selenides, which treated with thionyl chloride and triethylamine leading to (E)-1, 2-disubstituted ethenes in good yield.
文摘环己酮肟气相Beckmann重排反应RBS-1催化剂在高温反应时易失活,导致催化剂费用居高不下。为提高技术经济性,实现失活催化剂性能的高效恢复,系统研究RBS-1催化剂失活机理、再生手段及催化性能。采用N_(2)物理吸附-脱附技术考察催化剂失活前后与再生前后的比表面积及孔结构变化,并结合XRD、FT-IR和^(29)Si MAS NMR表征技术探讨了RBS-1催化剂在失活与再生过程中活性中心的演变规律。研究发现,失活RBS-1催化剂物相结构保持完好,但BET比表面积和孔体积均有明显降低,说明催化剂失活主要为积炭覆盖催化剂活性位点并堵塞孔道所致。针对积炭堵孔导致的催化剂失活问题,采用烧炭、含氮化合物改性处理构建巢式硅羟基活性位点等手段对失活催化剂进行再生研究。结果表明,在530℃下烧炭能将催化剂表面及孔道内的大部分积炭烧除,催化剂性能可恢复90%以上;对脱炭后的催化剂进一步改性处理,最优催化剂性能可恢复98%以上。
