The flotation behavior and adsorption mechanism of novel（1-hydroxy-2-methyl-2-octenyl） phosphonic acid（HEPA） to cassiterite were investigated by micro-flotation tests, zeta potential measurements, FTIR determinati...The flotation behavior and adsorption mechanism of novel（1-hydroxy-2-methyl-2-octenyl） phosphonic acid（HEPA） to cassiterite were investigated by micro-flotation tests, zeta potential measurements, FTIR determination and density functional theory（DFT） calculation. The flotation results demonstrated that HEPA exhibited superior collecting performance compared with styrene phosphonic acid（SPA）. The cassiterite recovery maintained above 90% over a wide pH range of 2-9 with 50 mg/L HEPA. The results of zeta potential measurement and FTIR detection indicated that the adsorption of HEPA onto cassiterite was mainly attributed to the chemisorption between HEPA monoanions and Sn species on mineral surfaces. The DFT calculation results demonstrated that HEPA monoanions owned higher HOMO energy and exhibited a better affinity to cassiterite than SPA, which provided very clear evidence for the stronger collecting power of HEPA presented in floatation test and zeta potential measurement.展开更多
In an attempt to identify bioactive compounds from a Tibetan medicinal herb Halenia elliptica, four known compounds were isolated. The crystal structure of the title compound 1-hydroxy-2,3,4,7-tetramethoxyxanthone was...In an attempt to identify bioactive compounds from a Tibetan medicinal herb Halenia elliptica, four known compounds were isolated. The crystal structure of the title compound 1-hydroxy-2,3,4,7-tetramethoxyxanthone was characterized by X-ray single-crystal structure analysis. The crystal belongs to monoclinic, space group P21/n with a = 7.155(2), b = 13.232(1), c = 16.037(2) ? b = 95.06(2), V = 1512.4(5) 3, C17H16O7, Mr = 332.30, Z = 4, Dc = 1.459 g/cm3, l(MoKa) = 0.71073 ? m(MoKa) = 1.15 cm-1 and F(000) = 696. It exhibits a characteristic approximate plane structure consisting of 13 carbon atoms (C(1) to C(13)) and all of the seven oxygen atoms (O(1) to O(7)). An intra-hydrogen bond was formed between O(6) and O(5)H(5).展开更多
目的探究阿尔茨海默病患者血清视锥蛋白样蛋白1(visinin-like protein,VILIP-1)、帕金森病蛋白2(Parkinson disease protein 2,PARK2)的表达及临床意义。方法选取2021年5月至2024年5月收治的96例阿尔兹海默病患者作为病例组,根据病情严...目的探究阿尔茨海默病患者血清视锥蛋白样蛋白1(visinin-like protein,VILIP-1)、帕金森病蛋白2(Parkinson disease protein 2,PARK2)的表达及临床意义。方法选取2021年5月至2024年5月收治的96例阿尔兹海默病患者作为病例组,根据病情严重程度进一步分为轻度组43例,中度组31例,重度组22例;另选择96例体检健康志愿者作为对照组。采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)对所有血清样本VILIP-1、PARK2表达水平进行检测;用Pearson相关分析阿尔茨海默病患者血清VILIP-1、PARK2表达水平与简易智力状态评分(Mini Mental State Examination,MMSE)评分及炎性因子的相关性;受试者工作特征(receiver operating characteristic,ROC)曲线,分析血清VILIP-1、PARK2水平对阿尔兹海默病的诊断价值。结果与对照组比较,病例组患者的血清VILIP-1及炎性因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-17(interleukin-17,IL-17)表达水平均升高,PARK2水平及MMSE评分均降低(P<0.05);随着阿尔茨海默病病情进展,血清VILIP-1表达水平逐渐升高,PARK2表达水平逐渐降低(P<0.05);Pearson相关分析结果显示阿尔茨海默病患者血清VILIP-1与MMSE评分呈负相关,与炎性因子TNF-α、IL-1β、IL-6、IL-17呈正相关(P<0.05);血清PARK2表达水平与MMSE评分呈正相关,与TNF-α、IL-1β、IL-6、IL-17呈负相关(P<0.05);ROC曲线结果表明,血清VILIP-1、PARK2联合诊断阿尔兹海默病患病的曲线下面积(area under the curve,AUC)值为0.883,显著大于VILIP-1(Z=2.531,P=0.011)和PARK2(Z=2.332,P=0.020)单独诊断。结论阿尔茨海默病患者血清VILIP-1表达水平升高,PARK2表达水平降低,二者与病情严重程度有关,同时检测两者的水平对阿尔茨海默病的诊断具有较高价值。展开更多
目的:探索通用转录因子ⅡE肽1(GTF2E1)在结直肠癌(CRC)中的表达水平及其对肿瘤细胞增殖的影响,以评估其作为潜在生物标志物和治疗靶点的价值。方法:通过收集并分析来自公共数据库的CRC样本数据,结合临床样本的免疫组化结果,利用单细胞...目的:探索通用转录因子ⅡE肽1(GTF2E1)在结直肠癌(CRC)中的表达水平及其对肿瘤细胞增殖的影响,以评估其作为潜在生物标志物和治疗靶点的价值。方法:通过收集并分析来自公共数据库的CRC样本数据,结合临床样本的免疫组化结果,利用单细胞测序技术和成簇规律间隔短回文重复序列(CRISPR)敲除实验,全面评价GTF2E1在CRC中的表达模式和作用。结果:GTF2E1在CRC组织中呈显著高表达,公共数据集、单细胞分析及内部临床样本检测结果一致。其诊断CRC的综合受试者工作特征曲线下面积为0.87,具有良好的诊断效能;CRISPR敲除实验显示,GTF 2 E 1敲除可显著抑制61种CRC细胞系的增殖。结论:GTF2E1在CRC的发生与增殖中起重要作用,有潜力成为CRC的生物标志物和治疗靶点。展开更多
基金Project(2013AA064102)supported by the 12th Five-year Plan of National Scientific and Technological Program of China
文摘The flotation behavior and adsorption mechanism of novel（1-hydroxy-2-methyl-2-octenyl） phosphonic acid（HEPA） to cassiterite were investigated by micro-flotation tests, zeta potential measurements, FTIR determination and density functional theory（DFT） calculation. The flotation results demonstrated that HEPA exhibited superior collecting performance compared with styrene phosphonic acid（SPA）. The cassiterite recovery maintained above 90% over a wide pH range of 2-9 with 50 mg/L HEPA. The results of zeta potential measurement and FTIR detection indicated that the adsorption of HEPA onto cassiterite was mainly attributed to the chemisorption between HEPA monoanions and Sn species on mineral surfaces. The DFT calculation results demonstrated that HEPA monoanions owned higher HOMO energy and exhibited a better affinity to cassiterite than SPA, which provided very clear evidence for the stronger collecting power of HEPA presented in floatation test and zeta potential measurement.
文摘In an attempt to identify bioactive compounds from a Tibetan medicinal herb Halenia elliptica, four known compounds were isolated. The crystal structure of the title compound 1-hydroxy-2,3,4,7-tetramethoxyxanthone was characterized by X-ray single-crystal structure analysis. The crystal belongs to monoclinic, space group P21/n with a = 7.155(2), b = 13.232(1), c = 16.037(2) ? b = 95.06(2), V = 1512.4(5) 3, C17H16O7, Mr = 332.30, Z = 4, Dc = 1.459 g/cm3, l(MoKa) = 0.71073 ? m(MoKa) = 1.15 cm-1 and F(000) = 696. It exhibits a characteristic approximate plane structure consisting of 13 carbon atoms (C(1) to C(13)) and all of the seven oxygen atoms (O(1) to O(7)). An intra-hydrogen bond was formed between O(6) and O(5)H(5).
文摘目的探究阿尔茨海默病患者血清视锥蛋白样蛋白1(visinin-like protein,VILIP-1)、帕金森病蛋白2(Parkinson disease protein 2,PARK2)的表达及临床意义。方法选取2021年5月至2024年5月收治的96例阿尔兹海默病患者作为病例组,根据病情严重程度进一步分为轻度组43例,中度组31例,重度组22例;另选择96例体检健康志愿者作为对照组。采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)对所有血清样本VILIP-1、PARK2表达水平进行检测;用Pearson相关分析阿尔茨海默病患者血清VILIP-1、PARK2表达水平与简易智力状态评分(Mini Mental State Examination,MMSE)评分及炎性因子的相关性;受试者工作特征(receiver operating characteristic,ROC)曲线,分析血清VILIP-1、PARK2水平对阿尔兹海默病的诊断价值。结果与对照组比较,病例组患者的血清VILIP-1及炎性因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-17(interleukin-17,IL-17)表达水平均升高,PARK2水平及MMSE评分均降低(P<0.05);随着阿尔茨海默病病情进展,血清VILIP-1表达水平逐渐升高,PARK2表达水平逐渐降低(P<0.05);Pearson相关分析结果显示阿尔茨海默病患者血清VILIP-1与MMSE评分呈负相关,与炎性因子TNF-α、IL-1β、IL-6、IL-17呈正相关(P<0.05);血清PARK2表达水平与MMSE评分呈正相关,与TNF-α、IL-1β、IL-6、IL-17呈负相关(P<0.05);ROC曲线结果表明,血清VILIP-1、PARK2联合诊断阿尔兹海默病患病的曲线下面积(area under the curve,AUC)值为0.883,显著大于VILIP-1(Z=2.531,P=0.011)和PARK2(Z=2.332,P=0.020)单独诊断。结论阿尔茨海默病患者血清VILIP-1表达水平升高,PARK2表达水平降低,二者与病情严重程度有关,同时检测两者的水平对阿尔茨海默病的诊断具有较高价值。
基金江西省自然科学基金项目(编号:20202BAB206075)江西省教育厅科技项目(编号:GJJ201202)江西中医药大学中西医结合一级学科平台(Discipline of Chinese and Western Integrative Medicine,Jiangxi University of Chinese Medicine)。
文摘目的:探索通用转录因子ⅡE肽1(GTF2E1)在结直肠癌(CRC)中的表达水平及其对肿瘤细胞增殖的影响,以评估其作为潜在生物标志物和治疗靶点的价值。方法:通过收集并分析来自公共数据库的CRC样本数据,结合临床样本的免疫组化结果,利用单细胞测序技术和成簇规律间隔短回文重复序列(CRISPR)敲除实验,全面评价GTF2E1在CRC中的表达模式和作用。结果:GTF2E1在CRC组织中呈显著高表达,公共数据集、单细胞分析及内部临床样本检测结果一致。其诊断CRC的综合受试者工作特征曲线下面积为0.87,具有良好的诊断效能;CRISPR敲除实验显示,GTF 2 E 1敲除可显著抑制61种CRC细胞系的增殖。结论:GTF2E1在CRC的发生与增殖中起重要作用,有潜力成为CRC的生物标志物和治疗靶点。
