Potato cyst nematodes(PCNs),Globodera rostochiensis,are an important group of quarantine plant-parasitic nematodes that pose a serious threat to the potato industry and global food security due to the lack of effectiv...Potato cyst nematodes(PCNs),Globodera rostochiensis,are an important group of quarantine plant-parasitic nematodes that pose a serious threat to the potato industry and global food security due to the lack of effective control measures.In this study,intercropping with Houttuynia cordata is shown to effectively reduce PCN populations while increasing potato yield.This effect is associated with the release of a key allelopathic volatile compound,1-decanol,which exhibits strong nematicidal activity(LC_(50)=31.5μg/mL)markedly exceeding that of the commercial nematicide avermectin(LC_(50)=63.8μg/mL).Meanwhile,root irrigation with 1-decanol significantly reduces PCN infection in potato.Mechanistically,1-decanol directly damages nematode surface structures,induces cellular apoptosis,and disrupts the oxidative stress regulation system,while also downregulating defense-related metabolic pathways in potato,thereby promoting the reallocation of metabolic resources from defense to growth.Moreover,1-decanol does not alter the overall diversity of the rhizosphere microbial community but selectively reduces the abundance of pathogenic taxa,such as Fusarium while recruiting beneficial microbiota to the potato rhizosphere,thereby limiting synergistic infections by pathogens and nematodes.These data suggest that intercropping with H.cordata suppresses PCNs and enhances potato yield through the antimicrobial activity of its allelochemicals and their effects on nematodes,the rhizosphere microbiome,and host physiology.Ultimately,intercropping with H.cordata provides a sustainable strategy for PCN management.展开更多
In this work, the individual and combined effects of the extractant, surfactant and modifier concentrations on the droplet coalescence time of the primary emulsion in the liquid surfactant membrane extraction process ...In this work, the individual and combined effects of the extractant, surfactant and modifier concentrations on the droplet coalescence time of the primary emulsion in the liquid surfactant membrane extraction process were evaluated, through emulsification experiments. Adogen 464 was used as extractant (carrier), and Escaid 110, as diluent. Two systems were studied. The first one composed by the extractant, the surfactant and the diluent, and the second one composed by the same reagents, but with the addition of 1-decanol as modifier. It was observed that, when the modifier is not present in the membrane phase, the surfactant not only stabilizes the primary emulsion, but, apparently, it also plays a role similar to that of the alcohol, promoting the solvation of the amine in a low polarity diluent. Furthermore, the extractant, a quaternary amine, helps to stabilize the primary emulsion in systems without a modifier. For membrane phases consisting of 1 or 5% w/w of Adogen 464 and 2% or 5% w/w of ECA 4360, a concentration of 3% w/w of 1-decanol was sufficient to promote the solvation of Adogen 464 in Escaid 110 and to obtain a low droplet coalescence time.展开更多
基金supported by the National Key Research and Development Program of China(2023YFD1400404)the National Natural Science Foundation of China(32060622)the Guizhou Provincial Foundation for Excellent Scholars Program(GCC[2023]069).
文摘Potato cyst nematodes(PCNs),Globodera rostochiensis,are an important group of quarantine plant-parasitic nematodes that pose a serious threat to the potato industry and global food security due to the lack of effective control measures.In this study,intercropping with Houttuynia cordata is shown to effectively reduce PCN populations while increasing potato yield.This effect is associated with the release of a key allelopathic volatile compound,1-decanol,which exhibits strong nematicidal activity(LC_(50)=31.5μg/mL)markedly exceeding that of the commercial nematicide avermectin(LC_(50)=63.8μg/mL).Meanwhile,root irrigation with 1-decanol significantly reduces PCN infection in potato.Mechanistically,1-decanol directly damages nematode surface structures,induces cellular apoptosis,and disrupts the oxidative stress regulation system,while also downregulating defense-related metabolic pathways in potato,thereby promoting the reallocation of metabolic resources from defense to growth.Moreover,1-decanol does not alter the overall diversity of the rhizosphere microbial community but selectively reduces the abundance of pathogenic taxa,such as Fusarium while recruiting beneficial microbiota to the potato rhizosphere,thereby limiting synergistic infections by pathogens and nematodes.These data suggest that intercropping with H.cordata suppresses PCNs and enhances potato yield through the antimicrobial activity of its allelochemicals and their effects on nematodes,the rhizosphere microbiome,and host physiology.Ultimately,intercropping with H.cordata provides a sustainable strategy for PCN management.
基金acknowledge CAPES,CNPq,FAPEMIG and PRPq by the financial support.
文摘In this work, the individual and combined effects of the extractant, surfactant and modifier concentrations on the droplet coalescence time of the primary emulsion in the liquid surfactant membrane extraction process were evaluated, through emulsification experiments. Adogen 464 was used as extractant (carrier), and Escaid 110, as diluent. Two systems were studied. The first one composed by the extractant, the surfactant and the diluent, and the second one composed by the same reagents, but with the addition of 1-decanol as modifier. It was observed that, when the modifier is not present in the membrane phase, the surfactant not only stabilizes the primary emulsion, but, apparently, it also plays a role similar to that of the alcohol, promoting the solvation of the amine in a low polarity diluent. Furthermore, the extractant, a quaternary amine, helps to stabilize the primary emulsion in systems without a modifier. For membrane phases consisting of 1 or 5% w/w of Adogen 464 and 2% or 5% w/w of ECA 4360, a concentration of 3% w/w of 1-decanol was sufficient to promote the solvation of Adogen 464 in Escaid 110 and to obtain a low droplet coalescence time.
