植物器官的大小调控是一个重要的生物学过程,直接影响农作物产量。目前对于植物器官大小调控的研究集中在转录调控、激素信号通路及蛋白质合成与修饰等多个水平和途径,但具体的分子机制并不是很清晰。李云海实验室前期研究发现了一个器...植物器官的大小调控是一个重要的生物学过程,直接影响农作物产量。目前对于植物器官大小调控的研究集中在转录调控、激素信号通路及蛋白质合成与修饰等多个水平和途径,但具体的分子机制并不是很清晰。李云海实验室前期研究发现了一个器官大小的关键调控基因UBIQUITIN SPECIFIC PROTEASE 15(UBP15)/SUPPRESSOR2 OF DA1(SOD2)。sod2-1突变体由于UBP15基因缺失导致器官变小。UBP15作为DA1的底物,其蛋白稳定性受DA1的调节,但其下游通路并不清楚。为了进一步了解UBP15调控器官大小的分子机制,通过EMS诱变筛选分离得到了sod2-1的抑制突变体sus40-1D。sus40-1D sod2-1突变体与sod2-1植株相比,子叶、花瓣和种子面积显著增大。遗传分析表明sus40-1D为显性突变。基因组重测序及连锁分析鉴定出2个与sus40-1D紧密连锁的候选基因:At1g05820和At1g08470。At1g05820的突变发生在内含子区域,而At1g08470的突变发生在外显子区。本研究发现了一个调控器官大小的突变体sus40-1D,对该突变体的进一步研究将有助于解析植物器官大小的调控机制。展开更多
To investigate the validity of two dynamic modulus predictive models（ Witczak 1-37 A viscosity-based model and Witczak 1-40 D shear modulus-based model） in the context of Jiangsu, and evaluate the effect of differen...To investigate the validity of two dynamic modulus predictive models（ Witczak 1-37 A viscosity-based model and Witczak 1-40 D shear modulus-based model） in the context of Jiangsu, and evaluate the effect of different mixture design variables（ aggregate gradations, binder type, and volumetric properties） on dynamic modulus E＊, asphalt mixtures commonly used in the local surface layer, including Sup-13 and AC-13, are prepared in the laboratory and their dynamic modulus E＊values are predicted based on the above mentioned models. The corresponding asphalt tests, including viscosity and dynamic shear modulus tests, are also carried out to obtain the prediction model parameters. The test results showthat binder type and asphalt content have a significant impact on dynamic modulus.There is a good correlation between the E＊values based on above two predictive models and the measured E＊, while a relatively lower bias can be expected from Witczak 1-37 A model. The test results can be used for the calibration of dynamic modulus with higher accuracy.展开更多
文摘植物器官的大小调控是一个重要的生物学过程,直接影响农作物产量。目前对于植物器官大小调控的研究集中在转录调控、激素信号通路及蛋白质合成与修饰等多个水平和途径,但具体的分子机制并不是很清晰。李云海实验室前期研究发现了一个器官大小的关键调控基因UBIQUITIN SPECIFIC PROTEASE 15(UBP15)/SUPPRESSOR2 OF DA1(SOD2)。sod2-1突变体由于UBP15基因缺失导致器官变小。UBP15作为DA1的底物,其蛋白稳定性受DA1的调节,但其下游通路并不清楚。为了进一步了解UBP15调控器官大小的分子机制,通过EMS诱变筛选分离得到了sod2-1的抑制突变体sus40-1D。sus40-1D sod2-1突变体与sod2-1植株相比,子叶、花瓣和种子面积显著增大。遗传分析表明sus40-1D为显性突变。基因组重测序及连锁分析鉴定出2个与sus40-1D紧密连锁的候选基因:At1g05820和At1g08470。At1g05820的突变发生在内含子区域,而At1g08470的突变发生在外显子区。本研究发现了一个调控器官大小的突变体sus40-1D,对该突变体的进一步研究将有助于解析植物器官大小的调控机制。
基金The Specialized Research Fund for the Doctoral Program of Higher Education of China(No.20120092110053)
文摘To investigate the validity of two dynamic modulus predictive models（ Witczak 1-37 A viscosity-based model and Witczak 1-40 D shear modulus-based model） in the context of Jiangsu, and evaluate the effect of different mixture design variables（ aggregate gradations, binder type, and volumetric properties） on dynamic modulus E＊, asphalt mixtures commonly used in the local surface layer, including Sup-13 and AC-13, are prepared in the laboratory and their dynamic modulus E＊values are predicted based on the above mentioned models. The corresponding asphalt tests, including viscosity and dynamic shear modulus tests, are also carried out to obtain the prediction model parameters. The test results showthat binder type and asphalt content have a significant impact on dynamic modulus.There is a good correlation between the E＊values based on above two predictive models and the measured E＊, while a relatively lower bias can be expected from Witczak 1-37 A model. The test results can be used for the calibration of dynamic modulus with higher accuracy.
