Background:Ovarian cancer(OC)is a representative malignancy of the female reproductive system,with a poor prognosis.Long non-coding RNAs(lncRNAs)crucially affect tumor development.This study aimed to identify lncRNAs ...Background:Ovarian cancer(OC)is a representative malignancy of the female reproductive system,with a poor prognosis.Long non-coding RNAs(lncRNAs)crucially affect tumor development.This study aimed to identify lncRNAs that potentially participated in OC.Methods:LncRNA expression in cells and tissues was quantified using reverse transcription-quantitative PCR,while fluorescence in situ hybridization determined their cellular localization.Various in vitro assays,together with a mouse xenograft model,were employed to elucidate the function of CYMP antisense RNA 1(CYMP-AS1)in OC.The molecular mechanisms underlying CYMP-AS1 regulation were investigated through RNA pull-down and immunoprecipitation assays,immunofluorescence staining,western blotting,and mRNA stability assays.Results:This study identified a previously unreported lncRNA,CYMP-AS1,which exhibits increased expression in the cytoplasm of OC tissues and cells.Knockout of CYMP-AS1 reduced the OC cell proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT).CYMP-AS1 directly interacts with heterogeneous nuclear ribonucleoprotein M(hnRNPM),inducing its intracellular translocation and reducing the stability of Axis inhibition protein 2(AXIN2)mRNA.This process ultimately elevated the expression of Wnt/β-catenin signaling pathway-related proteins.Conclusion:This study confirms CYMP-AS1 as a novel biomarker in OC progression and suggests that the CYMP-AS1/hnRNPM/AXIN2 axis may offer an innovative strategy for OC treatment.展开更多
基金funded by the National Natural Science Foundation of China(No.8207286).
文摘Background:Ovarian cancer(OC)is a representative malignancy of the female reproductive system,with a poor prognosis.Long non-coding RNAs(lncRNAs)crucially affect tumor development.This study aimed to identify lncRNAs that potentially participated in OC.Methods:LncRNA expression in cells and tissues was quantified using reverse transcription-quantitative PCR,while fluorescence in situ hybridization determined their cellular localization.Various in vitro assays,together with a mouse xenograft model,were employed to elucidate the function of CYMP antisense RNA 1(CYMP-AS1)in OC.The molecular mechanisms underlying CYMP-AS1 regulation were investigated through RNA pull-down and immunoprecipitation assays,immunofluorescence staining,western blotting,and mRNA stability assays.Results:This study identified a previously unreported lncRNA,CYMP-AS1,which exhibits increased expression in the cytoplasm of OC tissues and cells.Knockout of CYMP-AS1 reduced the OC cell proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT).CYMP-AS1 directly interacts with heterogeneous nuclear ribonucleoprotein M(hnRNPM),inducing its intracellular translocation and reducing the stability of Axis inhibition protein 2(AXIN2)mRNA.This process ultimately elevated the expression of Wnt/β-catenin signaling pathway-related proteins.Conclusion:This study confirms CYMP-AS1 as a novel biomarker in OC progression and suggests that the CYMP-AS1/hnRNPM/AXIN2 axis may offer an innovative strategy for OC treatment.
