目的探究LINC00511调节miR-150-5p/聚梳组蛋白家族指蛋白1(PHD family finger protein 1,PHF1)轴对结直肠癌细胞增殖、凋亡和上皮-间充质转化(epithelial-mesenchymal transformation,EMT)的影响。方法收集2021年3月—2023年3月本院24...目的探究LINC00511调节miR-150-5p/聚梳组蛋白家族指蛋白1(PHD family finger protein 1,PHF1)轴对结直肠癌细胞增殖、凋亡和上皮-间充质转化(epithelial-mesenchymal transformation,EMT)的影响。方法收集2021年3月—2023年3月本院24例行手术治疗的结直肠癌患者的癌组织和癌旁组织;培养结直肠癌细胞株SW480并随机分为正常对照组(Ctrl组)、si-NC组、si-LINC00511组、si-LINC00511+inhibitor NC组、si-LINC00511+miR-150-5p inhibitor组。采用RT-qPCR法检测结直肠癌患者癌组织和癌旁组织以及SW480细胞的LINC00511、miR-150-5p和PHF1 mRNA表达水平;CCK-8法检测细胞增殖能力;Transwell实验检测细胞侵袭和迁移能力;流式细胞术检测细胞凋亡;免疫印迹法检测PHF1以及凋亡、EMT相关蛋白表达。双荧光素酶报告基因实验分别验证LINC00511与miR-150-5p、miR-150-5p和PHF1的关系。结果与癌旁组织相比,结直肠癌患者癌组织中LINC00511、PHF1 mRNA表达增高,而miR-150-5p表达降低(P<0.001)。与si-NC组SW480细胞相比,si-LINC00511组SW480细胞的LINC00511表达、细胞增殖(OD_(450)值)、细胞迁移和侵袭数目以及Bcl-2、PHF1、N-cadherin、vimentin蛋白表达水平显著降低(P<0.01),miR-150-5p表达水平、细胞凋亡率以及Bax和Ecadherin蛋白表达水平显著升高(P<0.001)。与si-LINC00511+inhibitor NC组SW480细胞相比,si-LINC00511+miR-150-5p inhibitor组的相应指标变化与上述相反(P<0.001)。LINC00511靶向负调控miR-150-5p表达,miR-150-5p靶向负调控PHF1表达。结论敲低LINC00511可能通过促进miR-150-5p的表达,进而抑制PHF1的表达,对结直肠癌细胞增殖、EMT起到抑制作用,并诱导结直肠癌细胞凋亡。展开更多
目的探讨神经胶质瘤患者血浆外泌体长链非编码RNA LINC00511(lncRNA LINC00511)的表达水平及临床意义。方法选取2012 01-2016 04郑州人民医院收治的82例神经胶质瘤患者为研究对象,选取同期80例体检健康者为对照组,采用实时荧光定量PCR(q...目的探讨神经胶质瘤患者血浆外泌体长链非编码RNA LINC00511(lncRNA LINC00511)的表达水平及临床意义。方法选取2012 01-2016 04郑州人民医院收治的82例神经胶质瘤患者为研究对象,选取同期80例体检健康者为对照组,采用实时荧光定量PCR(qRT PCR)检测所有受试者血浆外泌体lncRNA LINC00511水平,观察受试者血浆外泌体lncRNA LINC00511表达情况,并探讨其与临床病理参数和预后的关系。结果 WHO Ⅲ~Ⅳ级、Ⅰ~Ⅱ级神经胶质瘤患者血浆外泌体lncRNA LINC00511水平明显高于对照组( P <0.05),Ⅲ~Ⅳ级患者高于Ⅰ~Ⅱ级患者( P <0.05);lncRNA LINC00511与患者WHO分级、KPS评分相关( P <0.05);lncRNA LINC00511高表达组生存率为34.09%,明显低于低表达组的65.79%( P <0.05),高表达组中位数生存时间为(42.00±3.60)个月,明显低于低表达组的(51.00±6.07)个月( P <0.05);多因素COX比例分析显示,高表达水平的lncRNA LINC00511、高WHO分级及KPS评分≤80分是神经胶质瘤患者预后不良的危险因素。结论 lncRNA LINC00511在神经胶质瘤患者血浆外泌体中表达上调,并与临床病理特征及预后密切相关。展开更多
BACKGROUND Gastric cancer(GC)is one of the most aggressive malignancies,with a high incidence and poor prognosis worldwide.Recently,accumulating evidence has illustrated that long noncoding RNAs(lnc RNAs)play pivotal ...BACKGROUND Gastric cancer(GC)is one of the most aggressive malignancies,with a high incidence and poor prognosis worldwide.Recently,accumulating evidence has illustrated that long noncoding RNAs(lnc RNAs)play pivotal roles in many cancers.It has been reported that LINC00511 contributes to tumorigenesis in various diseases.However,the role of LINC00511 in GC cell growth remains mostly unknown.AIM To determine whether the lnc RNA LINC00511 exerted its carcinogenic function in GC via the miR-124-3 p/PDK4 axis.METHODS Cell culture and transfection,RNA extraction and quantitative real-time PCR,CCK-8 assay,Colony formation assay,Luciferase reporter assay,RIP assay,RNA pull-down assay,and Western blot analysis were used to show expression and mechanisms of LINC00511 in GC progression and apoptosis.Rescue assays were performed to verify the relationships among LINC00511,miR-124-3 p and PDK4 further.RESULTS The expression of LINC00511 was remarkably upregulated in GC cells compared to that in corresponding normal cell lines.Compared to the controls,cell proliferation was inhibited,and cell apoptosis was increased upon LINC00511knockdown,demonstrating that LINC00511 influenced GC cell growth.An exploration of the molecular mechanism revealed that LINC00511 functioned as a molecular sponge of miR-124-3 p and that PDK4 was a downstream target of miR-124-3 p in GC.Rescue assays showed that the overexpression of PDK4 could partly restore the inhibitory function of si-LINC00511 in GC.CONCLUSION These data demonstrate that LINC00511 promotes gastric cancer cell growth by acting as a ce RNA to regulate the miR-124-3 p/PDK4 axis,which may be a promising therapeutic target for GC.展开更多
文摘目的探讨神经胶质瘤患者血浆外泌体长链非编码RNA LINC00511(lncRNA LINC00511)的表达水平及临床意义。方法选取2012 01-2016 04郑州人民医院收治的82例神经胶质瘤患者为研究对象,选取同期80例体检健康者为对照组,采用实时荧光定量PCR(qRT PCR)检测所有受试者血浆外泌体lncRNA LINC00511水平,观察受试者血浆外泌体lncRNA LINC00511表达情况,并探讨其与临床病理参数和预后的关系。结果 WHO Ⅲ~Ⅳ级、Ⅰ~Ⅱ级神经胶质瘤患者血浆外泌体lncRNA LINC00511水平明显高于对照组( P <0.05),Ⅲ~Ⅳ级患者高于Ⅰ~Ⅱ级患者( P <0.05);lncRNA LINC00511与患者WHO分级、KPS评分相关( P <0.05);lncRNA LINC00511高表达组生存率为34.09%,明显低于低表达组的65.79%( P <0.05),高表达组中位数生存时间为(42.00±3.60)个月,明显低于低表达组的(51.00±6.07)个月( P <0.05);多因素COX比例分析显示,高表达水平的lncRNA LINC00511、高WHO分级及KPS评分≤80分是神经胶质瘤患者预后不良的危险因素。结论 lncRNA LINC00511在神经胶质瘤患者血浆外泌体中表达上调,并与临床病理特征及预后密切相关。
文摘BACKGROUND Gastric cancer(GC)is one of the most aggressive malignancies,with a high incidence and poor prognosis worldwide.Recently,accumulating evidence has illustrated that long noncoding RNAs(lnc RNAs)play pivotal roles in many cancers.It has been reported that LINC00511 contributes to tumorigenesis in various diseases.However,the role of LINC00511 in GC cell growth remains mostly unknown.AIM To determine whether the lnc RNA LINC00511 exerted its carcinogenic function in GC via the miR-124-3 p/PDK4 axis.METHODS Cell culture and transfection,RNA extraction and quantitative real-time PCR,CCK-8 assay,Colony formation assay,Luciferase reporter assay,RIP assay,RNA pull-down assay,and Western blot analysis were used to show expression and mechanisms of LINC00511 in GC progression and apoptosis.Rescue assays were performed to verify the relationships among LINC00511,miR-124-3 p and PDK4 further.RESULTS The expression of LINC00511 was remarkably upregulated in GC cells compared to that in corresponding normal cell lines.Compared to the controls,cell proliferation was inhibited,and cell apoptosis was increased upon LINC00511knockdown,demonstrating that LINC00511 influenced GC cell growth.An exploration of the molecular mechanism revealed that LINC00511 functioned as a molecular sponge of miR-124-3 p and that PDK4 was a downstream target of miR-124-3 p in GC.Rescue assays showed that the overexpression of PDK4 could partly restore the inhibitory function of si-LINC00511 in GC.CONCLUSION These data demonstrate that LINC00511 promotes gastric cancer cell growth by acting as a ce RNA to regulate the miR-124-3 p/PDK4 axis,which may be a promising therapeutic target for GC.
