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Ligand-directed construction of cobalt-oxo cluster-based organic frameworks:Structural modulation,semiconductor,and antiferromagnetic properties
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作者 SHI Jinlian LIU Xiaoru XU Zhongxuan 《无机化学学报》 北大核心 2026年第1期45-54,共10页
Under hydrothermal and solvothermal conditions,two novel cobalt-based complexes,{[Co_(2)(CIA)(OH)(1,4-dtb)]·3.2H_(2)O}n(HU23)and{[Co_(2)(CIA)(OH)(1,4-dib)]·3.5H2O·DMF}n(HU24),were successfully construct... Under hydrothermal and solvothermal conditions,two novel cobalt-based complexes,{[Co_(2)(CIA)(OH)(1,4-dtb)]·3.2H_(2)O}n(HU23)and{[Co_(2)(CIA)(OH)(1,4-dib)]·3.5H2O·DMF}n(HU24),were successfully constructed by coordinatively assembling the semi-rigid multidentate ligand 5-(1-carboxyethoxy)isophthalic acid(H₃CIA)with the Nheterocyclic ligands 1,4-di(4H-1,2,4-triazol-4-yl)benzene(1,4-dtb)and 1,4-di(1H-imidazol-1-yl)benzene(1,4-dib),respectively,around Co^(2+)ions.Single-crystal X-ray diffraction analysis revealed that in both complexes HU23 and HU24,the CIA^(3-)anions adopt aκ^(7)-coordination mode,bridging six Co^(2+)ions via their five carboxylate oxygen atoms and one ether oxygen atom.This linkage forms tetranuclear[Co4(μ3-OH)2]^(6+)units.These Co-oxo cluster units were interconnected by CIA^(3-)anions to assemble into 2D kgd-type structures featuring a 3,6-connected topology.The 2D layers were further connected by 1,4-dtb and 1,4-dib,resulting in 3D pillar-layered frameworks for HU23 and HU24.Notably,despite the similar configurations of 1,4-dtb and 1,4-dib,differences in their coordination spatial orientations lead to topological divergence in the 3D frameworks of HU23 and HU24.Topological analysis indicates that the frameworks of HU23 and HU24 can be simplified into a 3,10-connected net(point symbol:(4^(10).6^(3).8^(2))(4^(3))_(2))and a 3,8-connected tfz-d net(point symbol:(4^(3))_(2)((4^(6).6^(18).8^(4)))),respectively.This structural differentiation confirms the precise regulatory role of ligands on the topology of metal-organic frameworks.Moreover,the ultraviolet-visible absorption spectra confirmed that HU23 and HU24 have strong absorption capabilities for ultraviolet and visible light.According to the Kubelka-Munk method,their bandwidths were 2.15 and 2.08 eV,respectively,which are consistent with those of typical semiconductor materials.Variable-temperature magnetic susceptibility measurements(2-300 K)revealed significant antiferromagnetic coupling in both complexes,with their effective magnetic moments decreasing markedly as the temperature lowered.CCDC:2457554,HU23;2457553,HU24. 展开更多
关键词 semi-rigid carboxylic acid ligands three-dimensional framework tetranuclear cobalt-oxo cluster semiconductor material antiferromagnetic magnetism
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老年急性脑梗死患者血清netrin-4、PARK7水平及其与早期神经功能恶化及预后的关系
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作者 张元超 王达岩 +1 位作者 刘庆昌 于航 《检验医学与临床》 2026年第1期74-79,共6页
目的探讨老年急性脑梗死(ACI)患者神经导向因子4(netrin-4)、人帕金森蛋白7(PARK7)水平及其与早期神经功能恶化及预后的关系。方法选取2021年2月至2023年12月该院收治的120例ACI患者作为观察组,根据美国国立卫生研究院卒中量表(NIHSS)... 目的探讨老年急性脑梗死(ACI)患者神经导向因子4(netrin-4)、人帕金森蛋白7(PARK7)水平及其与早期神经功能恶化及预后的关系。方法选取2021年2月至2023年12月该院收治的120例ACI患者作为观察组,根据美国国立卫生研究院卒中量表(NIHSS)评分将其分为重度组(NIHSS评分>15分)和轻度组(NIHSS评分≤15分);采用改良Rankin量表将其分为预后不良组(3~6级)和预后良好组(0~2级)。另选取同期在该院体检的120例健康体检者作为对照组。采用酶联免疫吸附试验检测所有研究对象血清netrin-4、PARK7水平;采用Pearson相关分析ACI患者血清netrin-4水平与PARK7水平的相关性;采用多因素Logistic回归分析ACI患者预后不良的影响因素;绘制受试者工作特征(ROC)曲线分析血清netrin-4、PARK7对ACI患者预后不良的预测价值。结果观察组血清PARK7、血肌酐水平均明显高于对照组,清蛋白(ALB)、血清netrin-4水平明显低于对照组,差异均有统计学意义(P<0.05);轻度组血清netrin-4水平明显高于重度组,血清PARK7水平明显低于重度组,差异均有统计学意义(P<0.05);预后不良组病灶最大径大于预后良好组,血肌酐、血清PARK7水平均高于预后良好组,ALB、血清netrin-4水平均低于预后良好组,差异均有统计学意义(P<0.05)。Pearson相关分析结果显示,ACI患者血清netrin-4水平与PARK7水平呈负相关(r=-0.517,P<0.001)。多因素Logistic回归分析结果显示,血清netrin-4水平升高是ACI患者预后不良的独立保护因素(P<0.05),较大的病灶最大径、血肌酐及ALB、血清PARK7水平升高均是ACI患者预后不良的独立危险因素(P<0.05)。ROC曲线分析结果显示,血清netrin-4、PARK7联合预测ACI患者预后不良的曲线下面积(AUC)为0.910,大于二者单独预测的AUC(0.854、0.837),差异均有统计学意义(Z=2.106、2.737,P<0.05)。结论ACI患者血清netrin-4水平降低,PARK7水平升高,且均与ACI患者预后有密切联系,可为临床预测ACI患者的预后提供一定参考价值。 展开更多
关键词 急性脑梗死 早期神经功能恶化 神经导向因子4 人帕金森蛋白7 预后
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Mapping editorial identity and thematic evolution in the Journal of Psychology in Africa(2008-2024):A meta-editorial framework analysis
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作者 Joon-ho Kim 《Journal of Psychology in Africa》 2026年第1期117-130,共14页
This study presents a reflective bibliometric review of 1457 peer-reviewed articles published in the Journal of Psychology in Africa(2008-2024,17 years),using a Meta-Editorial Mapping Framework(MEMF)analysis.The MEMF ... This study presents a reflective bibliometric review of 1457 peer-reviewed articles published in the Journal of Psychology in Africa(2008-2024,17 years),using a Meta-Editorial Mapping Framework(MEMF)analysis.The MEMF integrates citation metrics,keyword novelty ratios,TF-IDF weighting,and cluster-based topic modeling to trace long-term thematic trends and editorial evolution.Findings reveal sustained attention to foundational domains such as mental health,education,and identity,alongside a gradual integration of emergent themes including digital well-being,organizational behavior,and post-pandemic adaptation.Articles with moderate topical novelty(40%-60% new keywords)achieved the highest citation and usage metrics,suggesting that integrative innovation enhances scholarly impact.Clustering analyses indicate that the journal’s content forms overlapping conceptual domains rather than isolated silos.These insights contribute to editorial strategy,authorial positioning,and the future design of regional academic platforms.Moreover,the findings provide evidence supporting the use of the MEMF as a replicable tool for meta-editorial analysis across disciplinary and geographic boundaries. 展开更多
关键词 meta-editorial mapping framework(MEMF) topic evolution keyword novelty bibliometric analysis editorial strategy scholarly engagement Journal of Psychology in Africa
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Selective Synthesis of Bio-based Benzaldehyde Using Magnetic CoFe_(2)O_(4)@Biochar(HTR)Catalyst
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作者 Wanyun Tang Yuehui Luo +2 位作者 Can Zhu Nan Huang Quanxin Li 《Chinese Journal of Chemical Physics》 2026年第1期125-135,I0034-I0041,I0043,共20页
Developing green and efficient methods to acquire lignocellulose-based chemicals with high added value is beneficial for facilitating green chemistry and sustainable development.The goal of this study is to demonstrat... Developing green and efficient methods to acquire lignocellulose-based chemicals with high added value is beneficial for facilitating green chemistry and sustainable development.The goal of this study is to demonstrate that bio-based benzaldehyde,a noteworthy high-value chemical,is able to be directionally prepared from lignocellulosic biomass.This new control-lable transformation was materialized by uniting catalytic-pyrolysis of lignocellulose to toluene intermediate and catalytic oxidation of toluene intermediate to bio-based benzalde-hyde.This work also developed a highly active magnetic catalyst(CoFe_(2)O_(4)@Biochar(HTR)),achieving 77.1%benzaldehyde selectivity and 46.7%benzaldehyde yield using this catalyst.It was found that introducing the biochar carrier into the cobalt iron composite metal oxide cat-alyst enhanced hydroxyl radical formation and bio-based benzaldehyde synthesis.Based on catalyst characterizations and hydroxyl radical analysis,potential reaction mechanism for bio-based benzaldehyde synthesis was proposed.This strategy may provide a beneficial pathway for developing high-value bio-based chemical(benzaldehyde)using renewable lignocellulosic biomass. 展开更多
关键词 Lignocellulosic biomass Bio-based benzaldehyde Selective oxidation Toluene intermediate CoFe_(2)O_(4)@Biochar(HTR)catalyst
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P4LoF: Scheduling Loop-Free Multi-Flow Updates in Programmable Networks
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作者 Jiqiang Xia Qi Zhan +2 位作者 Le Tian Yuxiang Hu Jianhua Peng 《Computers, Materials & Continua》 2026年第1期1236-1254,共19页
The rapid growth of distributed data-centric applications and AI workloads increases demand for low-latency,high-throughput communication,necessitating frequent and flexible updates to network routing configurations.H... The rapid growth of distributed data-centric applications and AI workloads increases demand for low-latency,high-throughput communication,necessitating frequent and flexible updates to network routing configurations.However,maintaining consistent forwarding states during these updates is challenging,particularly when rerouting multiple flows simultaneously.Existing approaches pay little attention to multi-flow update,where improper update sequences across data plane nodes may construct deadlock dependencies.Moreover,these methods typically involve excessive control-data plane interactions,incurring significant resource overhead and performance degradation.This paper presents P4LoF,an efficient loop-free update approach that enables the controller to reroute multiple flows through minimal interactions.P4LoF first utilizes a greedy-based algorithm to generate the shortest update dependency chain for the single-flow update.These chains are then dynamically merged into a dependency graph and resolved as a Shortest Common Super-sequence(SCS)problem to produce the update sequence of multi-flow update.To address deadlock dependencies in multi-flow updates,P4LoF builds a deadlock-fix forwarding model that leverages the flexible packet processing capabilities of the programmable data plane.Experimental results show that P4LoF reduces control-data plane interactions by at least 32.6%with modest overhead,while effectively guaranteeing loop-free consistency. 展开更多
关键词 network management update consistency programmable data plane P4
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Lane Line Detection Method for Complex Road Scenes Based on DeepLabv3+and MobilenetV4
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作者 Yingkai Ge Jiasheng Zhang +3 位作者 Jiale Zhang Zhenguo Ma Yu Liu Lihua Wang 《Computers, Materials & Continua》 2026年第4期1341-1363,共23页
With the continuous development of artificial intelligence and computer vision technology,numerous deep learning-based lane line detection methods have emerged.DeepLabv3+,as a classic semantic segmentation model,has f... With the continuous development of artificial intelligence and computer vision technology,numerous deep learning-based lane line detection methods have emerged.DeepLabv3+,as a classic semantic segmentation model,has found widespread application in the field of lane line detection.However,the accuracy of lane line segmentation is often compromised by factors such as changes in lighting conditions,occlusions,and wear and tear on the lane lines.Additionally,DeepLabv3+suffers from high memory consumption and challenges in deployment on embedded platforms.To address these issues,this paper proposes a lane line detection method for complex road scenes based on DeepLabv3+and MobileNetV4(MNv4).First,the lightweight MNv4 is adopted as the backbone network,and the standard convolutions in ASPP are replaced with depthwise separable convolutions.Second,a polarization attention mechanism is introduced after the ASPP module to enhance the model’s generalization capability.Finally,the Simple Linear Iterative Clustering(SLIC)superpixel segmentation algorithmis employed to preserve lane line edge information.MNv4-DeepLabv3+was tested on the TuSimple and CULane datasets.On the TuSimple dataset,theMean Intersection over Union(MIoU)and Mean Pixel Accuracy(mPA)improved by 1.01%and 7.49%,respectively.On the CULane dataset,MIoU andmPA increased by 3.33%and 7.74%,respectively.Thenumber of parameters decreased from 54.84 to 3.19 M.Experimental results demonstrate that MNv4-DeepLabv3+significantly optimizes model parameter count and enhances segmentation accuracy. 展开更多
关键词 Deep learning lane line detection DeepLabv3+ MobilenetV4 SLIC
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基于MobileNetV4改进的YOLOv8目标检测算法研究
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作者 张宁 周云翀 +2 位作者 徐坤财 左超超 彭如镜 《集成技术》 2026年第2期91-103,共13页
为移动设备设计的轻量级卷积神经网络具有较快的推理速度,但受到自身网络局部性约束,即仅能在一个窗口区域内捕获局部信息,导致性能下降。引入自注意力机制虽然可以捕获全局信息,但会降低检测速度。针对上述问题,本文基于YOLOv8提出一... 为移动设备设计的轻量级卷积神经网络具有较快的推理速度,但受到自身网络局部性约束,即仅能在一个窗口区域内捕获局部信息,导致性能下降。引入自注意力机制虽然可以捕获全局信息,但会降低检测速度。针对上述问题,本文基于YOLOv8提出一种对硬件友好的MobileNetV4网络架构。该结构通过引入通用倒置瓶颈搜索块,融合了倒置瓶颈、ConvNext、Feed Forward网络及一种新型的额外深度卷积变体。同时,该结构还引入了动态上采样算子,改进了上采样操作,降低了模型使用GPU的内存和延迟。此外,本文改进了YOLOv8的检测头,通过引入动态检测头,将空间感知、尺度感知和任务感知融合到一个框架中,并在目标检测头中有效地应用注意力机制,提高检测性能和效率。实验结果表明,与次优模型YOLOv8n相比,YOLOv8n_M的平均精度均值mAP50~95提升了1.3%;在模型复杂度方面,YOLOv8n_M成功压缩了36%的模型规模(参数量缩减100万),同时将计算量降低26%(十亿次浮点运算GFLOPs减少2.4单位)。本文提出的YOLOv8n_M有效减少了模型的参数量和推理时间,并在一定程度上提高了模型在不同环境下的目标检测精度。 展开更多
关键词 YOLOv8 MobilenetV4 注意力机制 移动设备 动态检测头
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4D printing of reprocessable thiocyanate covalent adaptable networks with reconfigurable shape memory ability
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作者 Ting Xu Kexiang Chen +7 位作者 Zhiyuan He Chuanzhen Zhang Xiaoyu Li Ziyan Zhang Wenbo Fan Zhishen Ge Chenhui Cui Yanfeng Zhang 《Chinese Chemical Letters》 2026年第2期505-511,共7页
Shape memory polymers used in 4D printing only had one permanent shape after molding,which limited their applications in requiring multiple reconstructions and multifunctional shapes.Furthermore,the inherent stability... Shape memory polymers used in 4D printing only had one permanent shape after molding,which limited their applications in requiring multiple reconstructions and multifunctional shapes.Furthermore,the inherent stability of the triazine ring structure within cyanate ester(CE)crosslinked networks after molding posed significant challenges for both recycling,repairing,and degradation of resin.To address these obstacles,dynamic thiocyanate ester(TCE)bonds and photocurable group were incorporated into CE,obtaining the recyclable and 3D printable CE covalent adaptable networks(CANs),denoted as PTCE1.5.This material exhibits a Young's modulus of 810 MPa and a tensile strength of 50.8 MPa.Notably,damaged printed PTCE1.5 objects can be readily repaired through reprinting and interface rejoining by thermal treatment.Leveraging the solid-state plasticity,PTCE1.5 also demonstrated attractive shape memory ability and permanent shape reconfigurability,enabling its reconfigurable 4D printing.The printed PTCE1.5 hinges and a main body were assembled into a deployable and retractable satellite model,validating its potential application as a controllable component in the aerospace field.Moreover,printed PTCE1.5 can be fully degraded into thiol-modified intermediate products.Overall,this material not only enriches the application range of CE resin,but also provides a reliable approach to addressing environmental issue. 展开更多
关键词 4D Printing Dynamic thiocyanate ester bonds Covalent adaptable networks Cyanate ester resin Shape memory
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A magnetically recyclable CoFe_(2)O_(4)/BiOBr S-scheme heterojunction for efficient photocatalytic degradation of diuron:Performance,durability and mechanism exploration
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作者 Guie Li Chunyan Yang +4 位作者 Qiu Yang Qingzhu Zheng Menghan Li Jianhua Qu Guangshan Zhang 《Chinese Chemical Letters》 2026年第1期584-597,共14页
The excessive use of pesticides has exacerbated environmental pollution due to herbicide residues,while their persistent toxicity poses serious challenges to global ecological security.A magnetically recyclable CoFe_(... The excessive use of pesticides has exacerbated environmental pollution due to herbicide residues,while their persistent toxicity poses serious challenges to global ecological security.A magnetically recyclable CoFe_(2)O_(4)/BiOBr S-scheme heterojunctions was prepared by microwave-assisted co-precipitation method for photocatalytic degradation of Diuron(DUR) in water.The formation of S-scheme heterojunction enhances electron transfer and charge separation,which was demonstrated by free radical trapping,electrochemical experiments,and DFT calculations.The magnetic CoFe_(2)O_(4)/BiOBr catalysts can achieve 99.9 %removal of diuron in 50 min under visible light irradiation.Furthermore,the system maintains stable performance across a broad p H range(3-9),enabling adaptation to diverse water environments,effective elimination of multiple pollutants,and strong resistance to ionic interference.Using magnetic recovery,CoFe_(2)O_(4)/BiOBr exhibits a high removal rate of 99 % and a markedly low ion leaching rate(<20 μg/L) after six cycles photocatalytic process,confirming its excellent stability and durability.According to HPLCQTOF-MS and DFT calculation,the main ways of DUR degradation include dechlorinated hydroxylation,dealkylation and hydroxylation of aromatic ring and side chain.Toxicity analysis showed that the toxicity of the intermediates generated during degradation was generally lower than that of DUR.The magnetic CoFe_(2)O_(4)/BiOBr S-scheme heterojunction developed in this study exhibits excellent photocatalytic performance,high applicability,good stability,and durability,providing an effective magnetic for the removal of refractory pollutants. 展开更多
关键词 CoFe_(2)O_(4)/BiOBr DIURON DURABILITY DFT calculations Photocatalytic degradation
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Facile synthesis of porous Fe_(3)O_(4)/C composites derived from waste residues of soybean and electrolytic manganese for superior electromagnetic wave absorption
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作者 Zihao Liu Kaixiong Xiang +4 位作者 Yujia Nie Yiting Cheng Shaohua Jiang Han Chen Wei Zhou 《International Journal of Minerals,Metallurgy and Materials》 2026年第2期704-715,共12页
With growing concerns regarding electromagnetic pollution,low-cost,environmentally friendly,and high-performance electromagnetic wave absorption(EWA)materials have attracted significant attention.This paper reports on... With growing concerns regarding electromagnetic pollution,low-cost,environmentally friendly,and high-performance electromagnetic wave absorption(EWA)materials have attracted significant attention.This paper reports on the synthesis of porous Fe_(3)O_(4)/C composites that incorporate dielectric and magnetic loss mechanisms via the carbothermal reduction method and optimization of waste ratio to enhance EWA performance.The Fe_(3)O_(4)/C composites with 10wt%soybean residues(Fe_(3)O_(4)/C-10),demonstrated the best EWA performance,achieving the minimum reflection loss of−56.4 dB and a bandwidth of 2.14 GHz at a thickness of 2.23 mm.This enhanced EWA performance is primarily attributable to improved impedance matching and the synergistic effect between dielectric and magnetic losses.Furthermore,radar cross-sectional simulations confirmed the practical feasibility of the porous Fe_(3)O_(4)/C composites.This study proposes a viable strategy for utilizing soybean residue and electrolytic manganese residue,highlighting their potential applications in EWA. 展开更多
关键词 biomass-derived porous carbon electrolytic manganese residue Fe_(3)O_(4) electromagnetic wave absorption
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Temperature-dependent magnetotransport properties of Fe_(2)O_(4)/Pt heterostructure
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作者 Haomang He Ruijie Xu +2 位作者 Anke Song Zhongqiang Chen Xuefeng Wang 《Chinese Physics B》 2026年第1期280-285,共6页
We report on the growth of CoFe_(2)O_(4)/Pt heterostructure and their magnetotransport properties.The magnetoresistance under high magnetic fields exhibits a sign change when the temperature increases from 5 K to 10 K... We report on the growth of CoFe_(2)O_(4)/Pt heterostructure and their magnetotransport properties.The magnetoresistance under high magnetic fields exhibits a sign change when the temperature increases from 5 K to 10 K.The anomalous Hall resistance decreases as the temperature increases.Furthermore,angle-dependent magnetoresistance indicates that the observed magnetotransport behaviors originate from the competition between the spin Hall magnetoresistance and magnetic proximity effect. 展开更多
关键词 MAGnetOTRANSPORT magnetoresistance anomalous Hall effect Fe_(2)O_(4)/Pt heterostructure magnetic proximity effect
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防晒剂4-甲基苄亚基樟脑的安全评价及其监管情况
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作者 塔娜 朵慧 +3 位作者 张凤兰 余振喜 李波 苏哲 《日用化学工业(中英文)》 北大核心 2026年第2期245-251,共7页
文章旨在系统梳理4-甲基苄亚基樟脑的相关研究数据,对该成分作为化妆品防晒剂的安全性进行全面评估,为其在我国的使用和监管提供科学依据。通过文献调研,从理化特性、市场应用情况、人体吸收代谢机制以及安全性等多个维度对该物质展开... 文章旨在系统梳理4-甲基苄亚基樟脑的相关研究数据,对该成分作为化妆品防晒剂的安全性进行全面评估,为其在我国的使用和监管提供科学依据。通过文献调研,从理化特性、市场应用情况、人体吸收代谢机制以及安全性等多个维度对该物质展开研究。同时,回顾欧盟消费者安全科学委员会近二十年的评估历程与态度,对欧盟、美国、加拿大、澳大利亚、日本、东盟等国家(地区)的监管现状进行总结分析。经研究发现,体内实验数据表明4-甲基苄亚基樟脑对甲状腺系统可能存在一定影响,且有相关证据显示其具有潜在的内分泌干扰特性。尽管4-甲基苄亚基樟脑在国内化妆品市场中的使用频率呈现下降趋势,但相较于欧盟市场,其使用仍较为广泛,存在一定安全风险。因此,建议监管部门对该成分的安全性进行重新评估,并在此基础上为其制定科学合理的使用限量,保障公众用妆安全。 展开更多
关键词 4-甲基苄亚基樟脑 防晒剂 安全评价 监管
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抗青枯病茄子砧木新品种南茄砧4号的选育
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作者 伍壮生 李雪峤 +3 位作者 王小娟 廖道龙 吴月燕 高芳华 《中国蔬菜》 北大核心 2026年第3期213-216,共4页
南茄砧4号是以自交系HZ201702为母本,以南茄砧2号为父本配制而成的抗青枯病茄子砧木一代杂种。中熟,生长势较强,始花节位为第8~10节,主茎较短,侧枝上有密到极密的茸毛。花紫色,以簇生花序为主,每花序着花数3朵以上。果实卵形,纵径8~10 ... 南茄砧4号是以自交系HZ201702为母本,以南茄砧2号为父本配制而成的抗青枯病茄子砧木一代杂种。中熟,生长势较强,始花节位为第8~10节,主茎较短,侧枝上有密到极密的茸毛。花紫色,以簇生花序为主,每花序着花数3朵以上。果实卵形,纵径8~10 cm,横径5~6 cm,果皮紫褐色,萼片绿紫色,果面有条纹,单果质量90~110 g。种子肾形,千粒重约3.4 g,种皮浅黄色。嫁接亲和性好,高抗青枯病,适用于南方青枯病严重地区的茄子嫁接栽培。 展开更多
关键词 茄子砧木 南茄砧4 高抗青枯病
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基于TLR-4/MyD88/NF-κB信号通路探究温针灸干预膝骨关节炎的作用机制
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作者 王树东 董宝强 +5 位作者 盛泽伟 胡哲 于冬冬 王列 丁学峰 薛亚楠 《中华中医药学刊》 北大核心 2026年第2期24-27,I0008-I0012,共9页
目的基于Toll样受体-4(Toll-like receptors 4,TLR-4)/髓样分化因子88(Myeloid differentiation factor 88,MyD88)/核转录因子-κB(Nuclear transcription factor-kappa B,NF-κB)信号通路观察温针灸对膝骨关节炎(Knee osteoarthritis,K... 目的基于Toll样受体-4(Toll-like receptors 4,TLR-4)/髓样分化因子88(Myeloid differentiation factor 88,MyD88)/核转录因子-κB(Nuclear transcription factor-kappa B,NF-κB)信号通路观察温针灸对膝骨关节炎(Knee osteoarthritis,KOA)家兔模型的调控作用,探讨温针灸治疗膝骨关节炎的作用机制。方法健康大耳白兔40只,随机分为空白组、模型组、温针灸组、西药组,每组10只,以改良Hulth法建立膝骨关节炎模型,经4周干预后,随机处死两只,病理检查出现关节软骨表面不平整、炎性细胞浸润等改变即表明造模成功。采用HE染色法观察家兔膝关节形态学变化并进行光镜软骨检测评价标准(Mankin score,Mankin’s)评分;采用免疫组化法观察家兔膝关节软骨组织TLR-4、MyD88、NF-κB的表达,并进行半定量分析;采用酶联免疫吸附(Enzyme-linked immunosorbent assay,ELISA)法检测家兔血清中白细胞介素-6(Interleukin-6,IL-6)、白细胞介素-1β(Interleukin-1β,IL-1β)、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)的含量;采用Wes全自动蛋白质印迹定量分析系统检测软骨组织TLR-4、MyD88、NF-κB的蛋白表达水平。结果HE染色可见空白组家兔膝关节软骨组织平整,无任何病理变化。模型组软骨组织表面粗糙,软骨细胞呈簇状堆积或减少。温针灸组以及西药组软骨组织表面欠平整,少量裂隙,伴有少量炎性细胞浸润。与空白组相比,模型组血清中IL-6、IL-1β、TNF-α含量显著升高(P<0.05),与模型组相比,温针灸组和西药组血清中IL-6、IL-1β、TNF-α含量显著下降(P<0.05);与空白组相比,模型组软骨组织TLR-4、MyD88、NF-κB蛋白表达显著升高(P<0.05),与模型组相比,温针灸组和西药组软骨组织TLR-4、MyD88、NF-κB蛋白表达显著下降(P<0.05),且温针灸组效果更为显著。结论温针灸可以通过抑制TLR-4/MyD88/NF-κB信号通路被激活,减少下游IL-6、IL-1β、TNF-α的含量以缓解炎症反应,延缓病程进展,达到治疗KOA的目的,并能够对软骨组织起到一定程度的修复作用。 展开更多
关键词 膝骨关节炎 温针灸 炎症反应 TLR-4 MYD88 NF-ΚB
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视黄醇结合蛋白4荧光免疫层析检测技术的建立与应用研究
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作者 郑登滋 叶青 +3 位作者 张珠平 鄢雪梨 林菊珊 黄仁杰 《中国免疫学杂志》 北大核心 2026年第1期186-190,共5页
目的:建立血清视黄醇结合蛋白4(RBP4)的荧光免疫层析快速检测方法,并将其用于心血管疾病诊断研究。方法:采用双抗体夹心原理制备荧光免疫层析试纸条,系统评价线性范围、精密度、准确度、灵敏度、特异性。检测分析48例高血压样本、29例... 目的:建立血清视黄醇结合蛋白4(RBP4)的荧光免疫层析快速检测方法,并将其用于心血管疾病诊断研究。方法:采用双抗体夹心原理制备荧光免疫层析试纸条,系统评价线性范围、精密度、准确度、灵敏度、特异性。检测分析48例高血压样本、29例脑梗死样本及20例健康样本血清RBP4水平与心血管疾病相关性,并评估其诊断效能。结果:该方法对血清中RBP4的线性范围37.5~600 ng/mL,批内变异系数均<12%,批间变异系数均<13%,平均回收率95.6%,灵敏度为0.44 ng/mL,与含胆红素、甘油三酯和血红蛋白的干扰样本无明显交叉反应。相关性分析显示高血压和脑梗死患者血清RBP4水平高于健康对照,血清RBP4水平与高血压和脑梗死具有良好的相关性。ROC曲线分析显示,血清RBP4水平从健康人群中鉴别诊断高血压和脑梗死的AUC分别为0.914和0.850,提示血清RBP4水平诊断高血压的效能优于脑梗死。结论:建立了定量检测RBP4的荧光免疫层析法,有望为心血管疾病中高血压和脑梗死的早期筛查提供一种便捷的检测手段。 展开更多
关键词 视黄醇结合蛋白4 荧光免疫层析 心血管疾病
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种子休眠基因Sdr4的生物信息学分析与分子标记开发和应用
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作者 黄奇娜 姜鸿瑞 +3 位作者 杨婕 于坤宇 杨长登 梁燕 《中国水稻科学》 北大核心 2026年第1期61-71,共11页
【目的】水稻收获前穗发芽严重制约产量与品质形成,该性状主要受种子休眠基因调控。解析种子休眠分子机制对改良水稻穗萌抗性具有重要理论价值与育种意义。【方法】综合运用生物信息学分析、分子标记开发及标记辅助选择技术,系统解析种... 【目的】水稻收获前穗发芽严重制约产量与品质形成,该性状主要受种子休眠基因调控。解析种子休眠分子机制对改良水稻穗萌抗性具有重要理论价值与育种意义。【方法】综合运用生物信息学分析、分子标记开发及标记辅助选择技术,系统解析种子休眠基因Sdr4(Seed dormancy 4)的生物学功能,探究其分子标记在多基因型种质改良中的应用潜力。【结果】Sdr4启动子区含TATA-box等核心元件及ABA响应元件等多种顺式作用元件,其编码蛋白为低稳定性疏水蛋白(101~150氨基酸区段磷酸化修饰占比25.93%),三级结构以α-螺旋(15.50%)、β-折叠(15.50%)和无规则卷曲(66.37%)为主。3K单倍型数据库与AlphaFold分析表明Sdr4基因具有6种功能性突变的单倍型,且在强休眠种质Kasalath与弱休眠种质日本晴(Nipponbare)的Sdr4氨基酸序列存在多个位点差异。系统发育分析显示Sdr4在非洲栽培稻(Oryza glaberrima)与沼生菰(Zizania palustris)中具有高度保守性。通过Kasalath与日本晴的Sdr4等位变异,开发了Sdr4-KF/KR与Sdr4-PF/PR两对功能标记,可精准区分种子休眠强度。基于25个水稻品种的分子检测筛选出休眠性显著差异的种质,并利用上述标记成功创制了强休眠型中组18改良系。【结论】Sdr4通过调控种子休眠深度影响水稻穗萌抗性。分子标记筛选表明20个主栽品种均呈种子弱休眠表型,利用Sdr4特异性标记选育的K17与K88新品系具有显著穗萌抗性。本研究为Sdr4功能解析及水稻抗穗萌分子育种提供了理论支撑与技术储备。 展开更多
关键词 穗发芽 Sdr4 水稻 生物信息学 分子标记辅助选择
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仿木年轮结构Si_(3)N_(4)/Ni和316配副在海洋环境中的摩擦磨损性能研究
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作者 陈威 王朝 +4 位作者 周奥 李耶童 赵文卓 张建军 杨勇强 《陕西科技大学学报》 北大核心 2026年第1期152-160,共9页
本论文系统研究了仿木年轮Si_(3)N_(4)/Ni复合材料在海水中的摩擦磨损性能.在本研究中,利用热压烧结工艺制备仿木年轮的Si_(3)N_(4)/Ni复合材料,采用端面磨损试验机研究Si_(3)N_(4)/Ni复合材料与316不锈钢配副在人工海水环境中的摩擦磨... 本论文系统研究了仿木年轮Si_(3)N_(4)/Ni复合材料在海水中的摩擦磨损性能.在本研究中,利用热压烧结工艺制备仿木年轮的Si_(3)N_(4)/Ni复合材料,采用端面磨损试验机研究Si_(3)N_(4)/Ni复合材料与316不锈钢配副在人工海水环境中的摩擦磨损性能,利用扫描电子显微镜(SEM)观察与分析摩擦副表面的磨损形貌,采用EDS能谱仪和拉曼光谱来定性分析磨损面的表面元素与物质分布.结果表明,随着滑动速度增大,纯氮化硅和Si_(3)N_(4)/Ni复合材料与316不锈钢配副的摩擦系数均有所增大,在相同速度下,Si_(3)N_(4)/Ni复合材料与316配副表现出较好的摩擦磨损性能.当滑动速度为100 r/min时,Si_(3)N_(4)/Ni-316摩擦副表现出最优的摩擦学性能,摩擦系数为0.45,复合材料销磨损率为1.503×10^(-6)mm^(3)/Nm,金属盘磨损率为1.03×10^(-4)mm^(3)/Nm.Si_(3)N_(4)/Ni复合材料表现出相对较好的摩擦学性能,这主要归因于在复合材料摩擦表面发生了摩擦化学反应,生成硅胶(SiO_(2))和Ni_(2)SiO_(4)等物质,起到了减摩与润滑作用. 展开更多
关键词 Si_(3)N_(4)复合陶瓷 海水环境 摩擦磨损
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ACTN4、FOXQ1与子宫颈癌患者临床病理特征的关系及在预后评估的应用价值
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作者 马会 舒丽莎 +3 位作者 孙丽佳 殷晓霞 杨博 陈辛未 《中国妇产科临床杂志》 北大核心 2026年第1期11-14,共4页
目的探讨α-辅肌动蛋白4(ACTN4)、叉头框转录因子Q1(FOXQ1)与子宫颈癌患者临床病理特征及预后的关系。方法回顾性分析2017年1月至2020年1月在河北北方学院附属第一医院接受治疗的100例子宫颈癌患者的临床资料,采集其癌组织及经病理证实... 目的探讨α-辅肌动蛋白4(ACTN4)、叉头框转录因子Q1(FOXQ1)与子宫颈癌患者临床病理特征及预后的关系。方法回顾性分析2017年1月至2020年1月在河北北方学院附属第一医院接受治疗的100例子宫颈癌患者的临床资料,采集其癌组织及经病理证实无癌浸润的癌旁组织。采用免疫组化检测蛋白表达,分析其与临床病理参数的相关性;通过Kaplan-Meier、Cox回归及受试者工作特征曲线(ROC)评估两指标对患者预后的预测价值。结果ACTN4和FOXQ1在癌组织中的表达均上调(均P<0.05)。ACTN4与FOXQ1高表达组5年总生存率较低(均P<0.05),且与FIGO分期晚、低分化、深浸润及淋巴结转移独立相关(均P<0.05)。两者联合预测5年生存的AUC为0.905,灵敏度92.35%,特异度86.70%。结论ACTN4和FOXQ1高表达与子宫颈癌不良预后密切相关,可作为独立预后标志物,联合检测有助于提升预后评估效能。 展开更多
关键词 α-辅肌动蛋白4 叉头框转录因子Q1 子宫颈癌 病理特征 预后
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高良姜素调节TLR4/MD2信号通路对脑出血大鼠神经炎症的影响
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作者 曾理 张作军 +1 位作者 雷雨广 崔冬玲 《中国免疫学杂志》 北大核心 2026年第2期290-295,共6页
目的:探讨高良姜素(Gal)调节Toll样受体4(TLR4)/髓样分化蛋白-2(MD2)信号通路对脑出血大鼠神经炎症的影响。方法:SD大鼠随机分为正常组、脑出血组、Gal低剂量(Gal-L)组、Gal中剂量(Gal-M)组、Gal高剂量(Gal-H)组、Gal-H+TLR4激活剂(RS09... 目的:探讨高良姜素(Gal)调节Toll样受体4(TLR4)/髓样分化蛋白-2(MD2)信号通路对脑出血大鼠神经炎症的影响。方法:SD大鼠随机分为正常组、脑出血组、Gal低剂量(Gal-L)组、Gal中剂量(Gal-M)组、Gal高剂量(Gal-H)组、Gal-H+TLR4激活剂(RS09)组,每组18只。除正常组外,其他组大鼠均构建脑出血模型。造模成功后给药,1次/d,持续2周。检测大鼠神经功能损伤评分和脑含水量;HE染色检测血肿周围脑组织病理变化;免疫荧光染色检测血肿周围脑组织Iba-1和iNOS、Iba-1和CD206共表达情况;ELISA检测血肿周围脑组织IL-10、精氨酸酶1(Arg-1)、TNF-α、IL-1β水平;Western blot检测血肿周围脑组织TLR4、MD2蛋白。结果:与正常组相比,脑出血组脑组织细胞结构异常,表现为水肿、坏死等,可见大量炎症细胞浸润,神经功能损伤评分、脑含水量、Iba-1+iNOS共阳性小胶质细胞数量、TNF-α、IL-1β水平、TLR4、MD2蛋白水平升高,Iba-1+CD206共阳性小胶质细胞数量、IL-10、Arg-1水平降低(P<0.05);与脑出血组相比,Gal-L组、Gal-M组、Gal-H组细胞水肿、坏死及炎症细胞浸润现象有所缓解,神经功能损伤评分、脑含水量、Iba-1+iNOS共阳性小胶质细胞数量、TNF-α、IL-1β水平、TLR4、MD2蛋白水平降低,Iba-1+CD206共阳性小胶质细胞数量、IL-10、Arg-1水平升高(P<0.05);与Gal-H组相比,Gal-H+RS09组细胞水肿、坏死及炎症细胞浸润现象严重,神经功能损伤评分、脑含水量、Iba-1+iNOS共阳性小胶质细胞数量、TNF-α、IL-1β水平、TLR4、MD2蛋白水平升高,Iba-1+CD206共阳性小胶质细胞数量、IL-10、Arg-1水平降低(P<0.05)。结论:Gal改善脑出血大鼠神经炎症的机制可能与抑制TLR4/MD2信号通路有关。 展开更多
关键词 高良姜素 Toll样受体4/髓样分化蛋白-2信号通路 脑出血 神经炎症 小胶质细胞
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