Mesenchymal stem cells （MSCs） of nonembryonic origins possess the proliferation and multi-lineage differentiation potentials. It has been established that epigenetic mechanisms could be critical for determining the ...Mesenchymal stem cells （MSCs） of nonembryonic origins possess the proliferation and multi-lineage differentiation potentials. It has been established that epigenetic mechanisms could be critical for determining the fate of stem cells, and MSCs derived from different origins exhibited different expression profiles individually to a certain extent. In this study, ChiP-on-chip was used to generate genome-wide histone H3-Lys9 acetylation and dimethylation profiles at gene promoters in human bone marrow MSCs. We showed that modifications of histone H3-Lys9 at gene promoters correlated well with mRNA expression in human bone marrow MSCs. Functional analysis revealed that many key cellular pathways in human bone marrow MSC self-renewal, such as the canonical signaling pathways, cell cycle pathways and cytokine related pathways may be regulated by H3-Lys9 modifications. These data suggest that gene activation and silencing affected by H3-Lys9 acetylation and dimethylation, respectively, may be essential to the maintenance of human bone marrow MSC self-renewal and multi-potency.展开更多
一、英语中一些以-ly结尾的副词具有丰富的内涵,译成汉语时可以适当地加以发挥。1）Basically,the theory proposed,among otherthings,…就其基本内容而言,这一学说提出的论点包括：……（Basically译为＂就其基本内容而言＂）2）Enjoy...一、英语中一些以-ly结尾的副词具有丰富的内涵,译成汉语时可以适当地加以发挥。1）Basically,the theory proposed,among otherthings,…就其基本内容而言,这一学说提出的论点包括：……（Basically译为＂就其基本内容而言＂）2）Enjoyment of the untamed environment is notsomething that comes naturally to Chinese people.享受未经开发的环境,展开更多
基金the National Basic Research Program of China (No 2005CB522404 and 2006CB910506)the Program for Changjiang Scholars and Innovative Research Team (PCSIRT) in Universities (No IRT0519)the National Natural Science Founda-tion of China (No 30771232 and 30671184)
文摘Mesenchymal stem cells （MSCs） of nonembryonic origins possess the proliferation and multi-lineage differentiation potentials. It has been established that epigenetic mechanisms could be critical for determining the fate of stem cells, and MSCs derived from different origins exhibited different expression profiles individually to a certain extent. In this study, ChiP-on-chip was used to generate genome-wide histone H3-Lys9 acetylation and dimethylation profiles at gene promoters in human bone marrow MSCs. We showed that modifications of histone H3-Lys9 at gene promoters correlated well with mRNA expression in human bone marrow MSCs. Functional analysis revealed that many key cellular pathways in human bone marrow MSC self-renewal, such as the canonical signaling pathways, cell cycle pathways and cytokine related pathways may be regulated by H3-Lys9 modifications. These data suggest that gene activation and silencing affected by H3-Lys9 acetylation and dimethylation, respectively, may be essential to the maintenance of human bone marrow MSC self-renewal and multi-potency.
文摘一、英语中一些以-ly结尾的副词具有丰富的内涵,译成汉语时可以适当地加以发挥。1）Basically,the theory proposed,among otherthings,…就其基本内容而言,这一学说提出的论点包括：……（Basically译为＂就其基本内容而言＂）2）Enjoyment of the untamed environment is notsomething that comes naturally to Chinese people.享受未经开发的环境,
