In this paper, three kinds of snake venoms and four kinds of enzymes (phospholipase A 2, fibrinolytic enzyme, arginine esterase and L amino acid oxidase) isolated from the snake venom were analyzed. As the snake venom...In this paper, three kinds of snake venoms and four kinds of enzymes (phospholipase A 2, fibrinolytic enzyme, arginine esterase and L amino acid oxidase) isolated from the snake venom were analyzed. As the snake venom was different, the MALDI/TOF/MS showed difference. The MALDI/TOF/MS determination results could be affected by the concentrations of snake venom enzymes. And the mechanisms of desorption and ionization was also given in this study. By using MALDI/TOF/MS we obtained the accurate molecular weights and homogeneities of the enzymes. The apparent characteristics of the positive MALDI/TOF/MS of enzymes composed by two subunits were also given out. The results showed that MALDI/TOF/MS is an effective analytic method for discovering new components from snake venom complexes. And it is reliable to use this method to determine the molecular weights and purifies of protein molecules. [WT5HZ]展开更多
文摘In this paper, three kinds of snake venoms and four kinds of enzymes (phospholipase A 2, fibrinolytic enzyme, arginine esterase and L amino acid oxidase) isolated from the snake venom were analyzed. As the snake venom was different, the MALDI/TOF/MS showed difference. The MALDI/TOF/MS determination results could be affected by the concentrations of snake venom enzymes. And the mechanisms of desorption and ionization was also given in this study. By using MALDI/TOF/MS we obtained the accurate molecular weights and homogeneities of the enzymes. The apparent characteristics of the positive MALDI/TOF/MS of enzymes composed by two subunits were also given out. The results showed that MALDI/TOF/MS is an effective analytic method for discovering new components from snake venom complexes. And it is reliable to use this method to determine the molecular weights and purifies of protein molecules. [WT5HZ]
