A bidirectional labeling method was established to distinguish the proportions of HCO3- and CO2 utiliza- tion pathways of microalgae in Lake Hongfeng. The method was based on microalgae cultured in a medium by adding ...A bidirectional labeling method was established to distinguish the proportions of HCO3- and CO2 utiliza- tion pathways of microalgae in Lake Hongfeng. The method was based on microalgae cultured in a medium by adding equal concentrations of NaH13CO3 with different 613C values simultaneously. The inorganic carbon sources were quantified according to the stable carbon isotope composition in the treated microalgae. The effects of extracellular carbonic anhydrase （CAex） on the HCO3 and CO2 utilization pathways were distinguished using acetazolamide, a potent membrane-impermeable carbonic anhydrase inhibitor. The results show utilization of the added HCO3- was only 8% of the total carbon sources in karst lake. The proportion of the HCO3- utilization path- way was 52% of total inorganic carbon assimilation. Therefore, in the natural water of the karst area, the microalgae used less bicarbonate that preexisted in the aqueous medium than CO2 derived from the atmosphere. CAex increased the utilization of inorganic carbon from the atmosphere. The microalgae with CAex had greater carbon sequestration capacity in this karst area.展开更多
Objective:The purpose of the study is to investigate the technical points,effects and complications of fiducial marker implantation within target areas before the CyberKnife treatment on body malignant solid tumors.Me...Objective:The purpose of the study is to investigate the technical points,effects and complications of fiducial marker implantation within target areas before the CyberKnife treatment on body malignant solid tumors.Methods:Five hundred and four cases of patients with body malignant solid tumors accepted fiducial implantation within target areas under CT guidance before the treatment of CyberKnife.Observe the complications and effect.Results:Among the 504 cases,500 cases successfully accepted the implantation(a success rate of 99.2%).158 patients felt pain at the punctured sites and 3 patients had tachycardia.33 patients had abdominal pain after the surgery due to a small amount of bleeding in the needle passage during liver puncturing process.Among the 19 lung cancer patients who accepted lung paracentesis,1 case had light pneumothorax and 1 case got light haemothorax.Among the 453 patients who accepted liver paracentesis,6 had fiducial migration.Conclusion:The method of fiducial implantation within target areas before treating body malignant solid tumor with CyberKnife is minimally invasive and comparatively secure.展开更多
Having as a starting point of the problematic energy consumption of buildings, it studied the panorama of energy efficiency, emphasizing the Brazilian program of the INMETRO （Instituto Nacional de Metrologia, Normali...Having as a starting point of the problematic energy consumption of buildings, it studied the panorama of energy efficiency, emphasizing the Brazilian program of the INMETRO （Instituto Nacional de Metrologia, Normalizacao e Qualidade Industrial）, PROCEL “Edifica” （national program for energy efficiency in buildings）, contained in the norm RTQ-C （technical requirements of the quality for the level of energetic efficiency of commercial, service, and public buildings）. For a better understanding of the regulation, it elected a building, emblematic in the city of Passo Fundo, RS, Brazil, illustrating the perspective of the program. The building was classified in accordance with the two methodologies presented by the program： prescriptive and simulation, so it was possible to investigate the peculiarities of each methodology and the own regulation. After the application of the methodologies, we came to the conclusion that the prescriptive method is less accurate and requires more dedication by the evaluator to do all the calculations and surveys required, however, it is the fastest tool and accessible to the field professionals. The simulation on the other hand, is a more accurate methodology and reaches levels of analysis that the prescriptive method does not reach, but still is a tool that needs large initial financial contribution and prior knowledge.展开更多
AIM: To investigate genetics of two cases of colorectal tumor local recurrence and throw some light on the etiopathogenesis of anastomotic recurrence. METHODS: Two cases are presented: a 65-year-old female receiving t...AIM: To investigate genetics of two cases of colorectal tumor local recurrence and throw some light on the etiopathogenesis of anastomotic recurrence. METHODS: Two cases are presented: a 65-year-old female receiving two colonic resections for primary anastomotic recurrences within 21 mo, and a 57-year-old female undergoing two local excisions of recurrent anastomotic adenomas within 26 mo. A loss of heterozygosity (LOH) study of 25 microsatellite markers and a mutational analysis of genes BRAF , K-RAS and APC were performed in samples of neoplastic and normal colonic mucosa collected over the years. RESULTS: A diffuse genetic instability was present in all samples, including neoplastic and normal colonic mucosa. Two different patterns of genetic alterations (LOH at 5q21 and 18p11.23 in the first case, and LOH at 1p34 and 3p14 in the second) were found to be associated with carcinogenesis over the years. A role for the genes MYC-L (mapping at 1p34) and FIHT (mapping at 3p14.2) is suggested, whereas a role for APC (mapping at 5q21) is not shown. CONCLUSION: The study challenges the most credited intraluminal implantation and metachronous carcinogenesis theories, and suggests a persistent, patient-specific alteration as the trigger of colorectal cancer anastomotic recurrence.展开更多
In this paper, the author provides a representation of Italian public hospital facilitators （HFs） （clinics, regional or local community hospitals, and medical centers） by means of different types of networks. Movi...In this paper, the author provides a representation of Italian public hospital facilitators （HFs） （clinics, regional or local community hospitals, and medical centers） by means of different types of networks. Moving from the balance scorecards of HFs, the author has analyzed the representation of those data through the use of the minimum spanning tree （MST） and the planar maximally-filtered graph （PMFG）. This paper firstly examined the amount of information provided by the two networks and then run a sensitivity analysis of the networks by varying the elements of the balance scorecards to be considered. In this way, the author obtained a quite unusual representation of the overall economic situation of Italian HFs. Moreover, the author observed the emergence of patterns which in the author＇s opinion might help policy makers to realize a more efficient allocation of financial resources among the existing HFs.展开更多
Taking Beijing as an example and based on the census data,macro-level population statistical data and 2004~2013 marriage registration data,this paper makes a contrastive analysis on the characteristics and changing tr...Taking Beijing as an example and based on the census data,macro-level population statistical data and 2004~2013 marriage registration data,this paper makes a contrastive analysis on the characteristics and changing trends of local residents,migrants and temporary migrants. Relative to the local residents,the migrants generally have household registry of central districts and higher educational level and occupational layer. Temporary migrants have relatively complicated population structure but differentiate during the process of marriage matching. 'Two-place marriages'form a stable group of new permanent migrants and meanwhile imply a sizable group of latent dependent migrants. This will produce great infl uences on the general population structure and the aging process,which may also lead to some confl icts and problems. The government should be prepared with policy responses so as to promote the benign development of population diversifi cation in megalopolis.展开更多
Job burnout is a synthetic syndrome of emotional exhaustion, depersonalization and reduced personal accomplishment. It has a time-linked and situation-specific nature by developing gradually and fluctuating with chang...Job burnout is a synthetic syndrome of emotional exhaustion, depersonalization and reduced personal accomplishment. It has a time-linked and situation-specific nature by developing gradually and fluctuating with changes of environmental contributors. In this study, four related diary entries were analyzed narratively, written by a polytechnic teacher teaching English for General Purposes （EGP） in a semester, to verify the possible work- related situational factors causing iob burnout of EGP teachers in the Chinese context. The results revealed ten environmental burnout elements, including overload, severity of students＇ problem, lacking supervisors＇ support, limited iob autonomy, a stern hierarchical organizational system, and opaque operating rules. We interpreted the results in terms of teacher professional development and proposed that enhancing EGP teachers＇ professional identity and revaluing the EGP course in polytechnics were the basic ways to mitigate burnout and improve English teachers＇ professional development.展开更多
基金supported by the National Natural Sciences Foundation of China (U1612441)Foundation of Guizhou Province ([2014] 2131)Doctor Foundation of Guizhou Normal University (0514014)
文摘A bidirectional labeling method was established to distinguish the proportions of HCO3- and CO2 utiliza- tion pathways of microalgae in Lake Hongfeng. The method was based on microalgae cultured in a medium by adding equal concentrations of NaH13CO3 with different 613C values simultaneously. The inorganic carbon sources were quantified according to the stable carbon isotope composition in the treated microalgae. The effects of extracellular carbonic anhydrase （CAex） on the HCO3 and CO2 utilization pathways were distinguished using acetazolamide, a potent membrane-impermeable carbonic anhydrase inhibitor. The results show utilization of the added HCO3- was only 8% of the total carbon sources in karst lake. The proportion of the HCO3- utilization path- way was 52% of total inorganic carbon assimilation. Therefore, in the natural water of the karst area, the microalgae used less bicarbonate that preexisted in the aqueous medium than CO2 derived from the atmosphere. CAex increased the utilization of inorganic carbon from the atmosphere. The microalgae with CAex had greater carbon sequestration capacity in this karst area.
文摘Objective:The purpose of the study is to investigate the technical points,effects and complications of fiducial marker implantation within target areas before the CyberKnife treatment on body malignant solid tumors.Methods:Five hundred and four cases of patients with body malignant solid tumors accepted fiducial implantation within target areas under CT guidance before the treatment of CyberKnife.Observe the complications and effect.Results:Among the 504 cases,500 cases successfully accepted the implantation(a success rate of 99.2%).158 patients felt pain at the punctured sites and 3 patients had tachycardia.33 patients had abdominal pain after the surgery due to a small amount of bleeding in the needle passage during liver puncturing process.Among the 19 lung cancer patients who accepted lung paracentesis,1 case had light pneumothorax and 1 case got light haemothorax.Among the 453 patients who accepted liver paracentesis,6 had fiducial migration.Conclusion:The method of fiducial implantation within target areas before treating body malignant solid tumor with CyberKnife is minimally invasive and comparatively secure.
文摘Having as a starting point of the problematic energy consumption of buildings, it studied the panorama of energy efficiency, emphasizing the Brazilian program of the INMETRO （Instituto Nacional de Metrologia, Normalizacao e Qualidade Industrial）, PROCEL “Edifica” （national program for energy efficiency in buildings）, contained in the norm RTQ-C （technical requirements of the quality for the level of energetic efficiency of commercial, service, and public buildings）. For a better understanding of the regulation, it elected a building, emblematic in the city of Passo Fundo, RS, Brazil, illustrating the perspective of the program. The building was classified in accordance with the two methodologies presented by the program： prescriptive and simulation, so it was possible to investigate the peculiarities of each methodology and the own regulation. After the application of the methodologies, we came to the conclusion that the prescriptive method is less accurate and requires more dedication by the evaluator to do all the calculations and surveys required, however, it is the fastest tool and accessible to the field professionals. The simulation on the other hand, is a more accurate methodology and reaches levels of analysis that the prescriptive method does not reach, but still is a tool that needs large initial financial contribution and prior knowledge.
文摘AIM: To investigate genetics of two cases of colorectal tumor local recurrence and throw some light on the etiopathogenesis of anastomotic recurrence. METHODS: Two cases are presented: a 65-year-old female receiving two colonic resections for primary anastomotic recurrences within 21 mo, and a 57-year-old female undergoing two local excisions of recurrent anastomotic adenomas within 26 mo. A loss of heterozygosity (LOH) study of 25 microsatellite markers and a mutational analysis of genes BRAF , K-RAS and APC were performed in samples of neoplastic and normal colonic mucosa collected over the years. RESULTS: A diffuse genetic instability was present in all samples, including neoplastic and normal colonic mucosa. Two different patterns of genetic alterations (LOH at 5q21 and 18p11.23 in the first case, and LOH at 1p34 and 3p14 in the second) were found to be associated with carcinogenesis over the years. A role for the genes MYC-L (mapping at 1p34) and FIHT (mapping at 3p14.2) is suggested, whereas a role for APC (mapping at 5q21) is not shown. CONCLUSION: The study challenges the most credited intraluminal implantation and metachronous carcinogenesis theories, and suggests a persistent, patient-specific alteration as the trigger of colorectal cancer anastomotic recurrence.
文摘In this paper, the author provides a representation of Italian public hospital facilitators （HFs） （clinics, regional or local community hospitals, and medical centers） by means of different types of networks. Moving from the balance scorecards of HFs, the author has analyzed the representation of those data through the use of the minimum spanning tree （MST） and the planar maximally-filtered graph （PMFG）. This paper firstly examined the amount of information provided by the two networks and then run a sensitivity analysis of the networks by varying the elements of the balance scorecards to be considered. In this way, the author obtained a quite unusual representation of the overall economic situation of Italian HFs. Moreover, the author observed the emergence of patterns which in the author＇s opinion might help policy makers to realize a more efficient allocation of financial resources among the existing HFs.
文摘Taking Beijing as an example and based on the census data,macro-level population statistical data and 2004~2013 marriage registration data,this paper makes a contrastive analysis on the characteristics and changing trends of local residents,migrants and temporary migrants. Relative to the local residents,the migrants generally have household registry of central districts and higher educational level and occupational layer. Temporary migrants have relatively complicated population structure but differentiate during the process of marriage matching. 'Two-place marriages'form a stable group of new permanent migrants and meanwhile imply a sizable group of latent dependent migrants. This will produce great infl uences on the general population structure and the aging process,which may also lead to some confl icts and problems. The government should be prepared with policy responses so as to promote the benign development of population diversifi cation in megalopolis.
文摘Job burnout is a synthetic syndrome of emotional exhaustion, depersonalization and reduced personal accomplishment. It has a time-linked and situation-specific nature by developing gradually and fluctuating with changes of environmental contributors. In this study, four related diary entries were analyzed narratively, written by a polytechnic teacher teaching English for General Purposes （EGP） in a semester, to verify the possible work- related situational factors causing iob burnout of EGP teachers in the Chinese context. The results revealed ten environmental burnout elements, including overload, severity of students＇ problem, lacking supervisors＇ support, limited iob autonomy, a stern hierarchical organizational system, and opaque operating rules. We interpreted the results in terms of teacher professional development and proposed that enhancing EGP teachers＇ professional identity and revaluing the EGP course in polytechnics were the basic ways to mitigate burnout and improve English teachers＇ professional development.
