以松茸[Tricholoma matsutake(S.Ito et Imai)Sing.]超临界提取物为研究对象,采用超临界CO_(2)萃取法制得松茸超临界提取物,使用超高效液相色谱-串联质谱(UPLC-MS)初步分析其成分;通过酪氨酸酶抑制试验、小鼠黑色素瘤细胞(B16-F10)试验...以松茸[Tricholoma matsutake(S.Ito et Imai)Sing.]超临界提取物为研究对象,采用超临界CO_(2)萃取法制得松茸超临界提取物,使用超高效液相色谱-串联质谱(UPLC-MS)初步分析其成分;通过酪氨酸酶抑制试验、小鼠黑色素瘤细胞(B16-F10)试验以及斑马鱼试验,评价其美白功效。结果表明,松茸超临界提取物对酪氨酸酶有一定的抑制活性,在低质量浓度(24.39μg/mL)下抑制率为(23.02%±0.65%),抑制率随质量浓度升高而提升,抑制率最高为(33.96%±1.17%),并且在0.0125 mg/mL时,对B16-F10细胞黑色素分泌的抑制率为(31.14%±0.81%);对斑马鱼中的黑色素形成也具有显著的抑制作用,质量浓度为0.05 mg/mL时可抑制斑马鱼中黑色素(24.83%±0.96%)形成。综上,松茸超临界提取物具有作为美白功效原料的潜力,在美白、提亮肤色方面均有开发价值。展开更多
RAPD analysis was adopted to identify the Tricholoma matsutake and T. bakamatsutake. With the method of BSA, a polymorphic band of 870 bp linked to T. matsutake was amplified, and the SCAR marker was developed from th...RAPD analysis was adopted to identify the Tricholoma matsutake and T. bakamatsutake. With the method of BSA, a polymorphic band of 870 bp linked to T. matsutake was amplified, and the SCAR marker was developed from the polymorphic band by cloning, sequencing and designing primers. The SCAR marker only presented in the individuals of T. matsutake, thus, it can quickly and accurately identify T. matsutake and T. bakamatsutake at molecular level.展开更多
文摘RAPD analysis was adopted to identify the Tricholoma matsutake and T. bakamatsutake. With the method of BSA, a polymorphic band of 870 bp linked to T. matsutake was amplified, and the SCAR marker was developed from the polymorphic band by cloning, sequencing and designing primers. The SCAR marker only presented in the individuals of T. matsutake, thus, it can quickly and accurately identify T. matsutake and T. bakamatsutake at molecular level.
