The ability to knockdown the expression of an endogenous gene by RNAi has emerged as a powerful strategy for the rapid identification of specific gene functions. Vector-based constitutive expression of shRNA can resul...The ability to knockdown the expression of an endogenous gene by RNAi has emerged as a powerful strategy for the rapid identification of specific gene functions. Vector-based constitutive expression of shRNA can result in stable and efficient knockdown of target genes. However, constitutive expression of shRNA imposes major limitations when analyzing the fimction of genes whose expression is vital for the survival of an organism. Inducible RNAi systems can circumvent this limitation by enabling the inhibition of expression of an essential gene only when the inducing agent is present, and the level of knockdown of the essential gene can be controlled and adjusted by the concentration of inducing agent. In this review, we briefly summarize the recent development of various inducible RNAi systems and their potential applications in drug target validation.展开更多
Objective： To observe the effects of cytokine-induced killer （CIK） cells on the treatment of renal cell carcinoma. Methods： Twenty-eight postoperative cases with stage Ⅰ or stage Ⅱrenal cell carcinoma were admit...Objective： To observe the effects of cytokine-induced killer （CIK） cells on the treatment of renal cell carcinoma. Methods： Twenty-eight postoperative cases with stage Ⅰ or stage Ⅱrenal cell carcinoma were admitted in our hospital from January 2002 to June 2006, all cases were pathologically confirmed, and were divided into group A （18 cases） and group B （10 cases）. Group A was administrated 3-12 periods of CIK cells treatment combined with 5-7 cycles of IL-2 and INFα-2b, together with 4 cycles of chemotherapy （5-Fu ＋ CF）. Group B was given 4 cycles of chemotherapy （5-Fu ＋ CF） and 5-7 cycles of IL-2 and INFa-2b. Results： Three cases in group A had metastatic masses in two lungs within 1 year and died within 2 years postoperatively. The other 15 cases are still alive and in good health. Six cases in group B had metastatic masses in two lungs or/and in abdominal cavity within 1 year, and 4 of them died within 2 years. All the 6 cases died within 3 years. The other 4 cases are still alive and in good health. Conclusion： CIK cells are safe and effective for the treatment of stage I or stage II renal cell carcinoma, which should be further widely used.展开更多
Michoacan brings 2.1% of national GDP, which is 12.5% to agriculture, Livestock is the fourth activity in economic importance in the State and develops in 43% of the territory （58,643 km：） using 27% of the populati...Michoacan brings 2.1% of national GDP, which is 12.5% to agriculture, Livestock is the fourth activity in economic importance in the State and develops in 43% of the territory （58,643 km：） using 27% of the population economically active. Michoacfin ranks third in national cattle inventory （1,608,523 heads） with fifth place in meat production （145,221 ton/year）. The production model is extended cow-breed, subject to season and availability of food （few rains shortage of forage during drought）, with 0.60 production of breed/cow/year, and a marked traditionalism in the form of production; also a high dependency to food based on the grazing. The inadequate national public policy expressed in the paternalism has limited the adoption of strategies to contribute to the solution of this problem. Therefore this study objective was to design and validate a strategy to increase the number of cow breeding/cow/year to season reproduction program, based on the use of synthetic progesterone ＂melengestrol acetate＂ （MGA） as a technology available, secure, easy to use, low cost and likely to be adoptable to the traditional model of production. Work was done in the dry tropics in the municipalities of Churumuco and Tzitzio, Michoacfin State, Mdxico. Used 133 bovine females with crossbreeding Bos indicus, diagnosed as not pregnant, 6.7 and 6.38 years old respectively for each municipality. Oral doses of 0.05 rag/day/cow of MGA during nine days then exposed to the presence of male. Data were analyzed using analysis of variance of a single way. The results indicate that the delivery rate was 58.33% in Churumuco and 61.86% in Tzitzio. This delivery rate represents an increase of 14%-16% of born per year considering that treated females become pregnant with traditional production system management. We concluded that the MGA is an appropriate strategy to the traditional production of tropical dry in MichoacO, n system that increases the rate of births and is likely to be accepted despite the paternalistic public policy prevailing in the country.展开更多
Chongqing Zhong Yi Seed Co.,Ltd is one of the key enterprises of Chinese seed industry,an AAA credit rating enterprise,a national high-tech enterprise,and the leading agriculture industrialization enterprise,which is ...Chongqing Zhong Yi Seed Co.,Ltd is one of the key enterprises of Chinese seed industry,an AAA credit rating enterprise,a national high-tech enterprise,and the leading agriculture industrialization enterprise,which is bestowed with the national seed business cortificate and the right of seed export and import.展开更多
基金National Natural Science Foundation of China(Grant No.20852001)"985"Project Foundation(Grant No.985-2-126-121 )+1 种基金The Key Laboratory Grant(Grant No.20080104)National Basic Research Program of China(Grant No.973 Program,2010CB 12300)
文摘The ability to knockdown the expression of an endogenous gene by RNAi has emerged as a powerful strategy for the rapid identification of specific gene functions. Vector-based constitutive expression of shRNA can result in stable and efficient knockdown of target genes. However, constitutive expression of shRNA imposes major limitations when analyzing the fimction of genes whose expression is vital for the survival of an organism. Inducible RNAi systems can circumvent this limitation by enabling the inhibition of expression of an essential gene only when the inducing agent is present, and the level of knockdown of the essential gene can be controlled and adjusted by the concentration of inducing agent. In this review, we briefly summarize the recent development of various inducible RNAi systems and their potential applications in drug target validation.
文摘Objective： To observe the effects of cytokine-induced killer （CIK） cells on the treatment of renal cell carcinoma. Methods： Twenty-eight postoperative cases with stage Ⅰ or stage Ⅱrenal cell carcinoma were admitted in our hospital from January 2002 to June 2006, all cases were pathologically confirmed, and were divided into group A （18 cases） and group B （10 cases）. Group A was administrated 3-12 periods of CIK cells treatment combined with 5-7 cycles of IL-2 and INFα-2b, together with 4 cycles of chemotherapy （5-Fu ＋ CF）. Group B was given 4 cycles of chemotherapy （5-Fu ＋ CF） and 5-7 cycles of IL-2 and INFa-2b. Results： Three cases in group A had metastatic masses in two lungs within 1 year and died within 2 years postoperatively. The other 15 cases are still alive and in good health. Six cases in group B had metastatic masses in two lungs or/and in abdominal cavity within 1 year, and 4 of them died within 2 years. All the 6 cases died within 3 years. The other 4 cases are still alive and in good health. Conclusion： CIK cells are safe and effective for the treatment of stage I or stage II renal cell carcinoma, which should be further widely used.
文摘Michoacan brings 2.1% of national GDP, which is 12.5% to agriculture, Livestock is the fourth activity in economic importance in the State and develops in 43% of the territory （58,643 km：） using 27% of the population economically active. Michoacfin ranks third in national cattle inventory （1,608,523 heads） with fifth place in meat production （145,221 ton/year）. The production model is extended cow-breed, subject to season and availability of food （few rains shortage of forage during drought）, with 0.60 production of breed/cow/year, and a marked traditionalism in the form of production; also a high dependency to food based on the grazing. The inadequate national public policy expressed in the paternalism has limited the adoption of strategies to contribute to the solution of this problem. Therefore this study objective was to design and validate a strategy to increase the number of cow breeding/cow/year to season reproduction program, based on the use of synthetic progesterone ＂melengestrol acetate＂ （MGA） as a technology available, secure, easy to use, low cost and likely to be adoptable to the traditional model of production. Work was done in the dry tropics in the municipalities of Churumuco and Tzitzio, Michoacfin State, Mdxico. Used 133 bovine females with crossbreeding Bos indicus, diagnosed as not pregnant, 6.7 and 6.38 years old respectively for each municipality. Oral doses of 0.05 rag/day/cow of MGA during nine days then exposed to the presence of male. Data were analyzed using analysis of variance of a single way. The results indicate that the delivery rate was 58.33% in Churumuco and 61.86% in Tzitzio. This delivery rate represents an increase of 14%-16% of born per year considering that treated females become pregnant with traditional production system management. We concluded that the MGA is an appropriate strategy to the traditional production of tropical dry in MichoacO, n system that increases the rate of births and is likely to be accepted despite the paternalistic public policy prevailing in the country.
文摘Chongqing Zhong Yi Seed Co.,Ltd is one of the key enterprises of Chinese seed industry,an AAA credit rating enterprise,a national high-tech enterprise,and the leading agriculture industrialization enterprise,which is bestowed with the national seed business cortificate and the right of seed export and import.
