一、文本问题1900年,梅特兰(Frederic William Maitland)教授主持出版了一部名为《中世纪政治理论》(Political Theories of the Middle Age)的书,其中收录了基尔克(Otto von Gierke)《日耳曼社团法》(Das deutsche Genossenschaftsrec...一、文本问题1900年,梅特兰(Frederic William Maitland)教授主持出版了一部名为《中世纪政治理论》(Political Theories of the Middle Age)的书,其中收录了基尔克(Otto von Gierke)《日耳曼社团法》(Das deutsche Genossenschaftsrecht)第三卷中的一个章节,《日耳曼社团法》的第三卷大约在1900年之前20年的1881年首次出版。您现在所看到的这部作品讲述的是1500~1800年的各种现代政治理论,译自基尔克《日耳曼社团法》第四卷中的五个小节,第四卷首次出版于1913年,距今整整20年。[1]就年代顺序而言.展开更多
[Objective] This study aimed to explore the molecular mechanism of mulberry pigment metabolism regulation. [Method] Chalconesynthase（CHS） gene was cloned from Morus（Moraceae） in silico. The amino acid sequence, ph...[Objective] This study aimed to explore the molecular mechanism of mulberry pigment metabolism regulation. [Method] Chalconesynthase（CHS） gene was cloned from Morus（Moraceae） in silico. The amino acid sequence, physical and chemical properties, transmembrane structural domain, hydrophobicity/hydrophilicity,subcellular localization, secondary and tertiary structure of protein were predicted and analyzed by bioinformatics tools. [Result] The cDNA sequence of CHS gene was 1 365bp by splicing using the software DNAstar and it contained a complete ORF including 1 170 bp which encoded 389 amino acids. Bioinformatic analysis showed that CHS gene included specific peptide sequence RLMMYQQGCFAGGTVLR of chalcone synthase superfamily, but has no signal peptide, belonging to the non-secretory proteins, located inside of cytoplasm. Its molecular evolution is more conservative.[Conclusion] The results above provided foundation for the further studies of structure and function of CHS protein.展开更多
To keep the tunnel face stable is very important for tunnel construction.In this paper,the tunnel face stability under the advanced pipe was analyzed using the Winkler foundation model and rigid limit equilibrium.The ...To keep the tunnel face stable is very important for tunnel construction.In this paper,the tunnel face stability under the advanced pipe was analyzed using the Winkler foundation model and rigid limit equilibrium.The tunnel face deformation characteristics were also analyzed using the numerical simulation.The influence of parameters on the deflection of the pipe roof and the stability of the tunnel face were discussed.The results show that the tunnel face stability can be improved through increasing the pipe diameter,decreasing the initial displacement at the beginning of the pipe seat,and adopting the short round length and small excavation height.With the increase of tunnel burial depth,the safety factor of tunnel face stability first decreases,then increases,and then remains unchanged.The deformation at the center of the tunnel face is larger than the deformation at the surround sides and at the corner.The horizontal displacement varies little with the increasing of the pipe length.The horizontal displacement at the center of the tunnel face increases with the increase of the pipe ring spacing and the pipe longitudinal spacing.There is an optimum external angle.展开更多
[Objective] This study aimed to clone and analyze the sequence of CHS gene from Acer truncatum leaves. [Method] Using A. truncatum cultivars No.1-6 as experimental materials, total RNA was extracted from A. truncatum ...[Objective] This study aimed to clone and analyze the sequence of CHS gene from Acer truncatum leaves. [Method] Using A. truncatum cultivars No.1-6 as experimental materials, total RNA was extracted from A. truncatum leaves with the modified CTAB method. CHS gene sequences were downloaded from the NCBI and aligned by BLAST. Degenerate primers were designed by DNAMAN and Primer- premier5 to amplify the target band. CHS gene fragment was amplified by RT-PCR and ligated to pMD18-T vector. The identified positive colonies were sequenced. [Result] A 1 365 bp fragment was amplified. Sequence analysis suggested that the obtained fragment encoded 365 amino acids and shared above 90% homology to nucleotide sequence of CHS gene from A. palmatum and A. [Conclusion] In this study, CHS gene was successfully cloned from A. truncatum for the first time, which laid the foundation for efficient utilization of CHS gene.展开更多
文摘一、文本问题1900年,梅特兰(Frederic William Maitland)教授主持出版了一部名为《中世纪政治理论》(Political Theories of the Middle Age)的书,其中收录了基尔克(Otto von Gierke)《日耳曼社团法》(Das deutsche Genossenschaftsrecht)第三卷中的一个章节,《日耳曼社团法》的第三卷大约在1900年之前20年的1881年首次出版。您现在所看到的这部作品讲述的是1500~1800年的各种现代政治理论,译自基尔克《日耳曼社团法》第四卷中的五个小节,第四卷首次出版于1913年,距今整整20年。[1]就年代顺序而言.
基金Supported by the National Natural Science Foundation of China(31072087)~~
文摘[Objective] This study aimed to explore the molecular mechanism of mulberry pigment metabolism regulation. [Method] Chalconesynthase（CHS） gene was cloned from Morus（Moraceae） in silico. The amino acid sequence, physical and chemical properties, transmembrane structural domain, hydrophobicity/hydrophilicity,subcellular localization, secondary and tertiary structure of protein were predicted and analyzed by bioinformatics tools. [Result] The cDNA sequence of CHS gene was 1 365bp by splicing using the software DNAstar and it contained a complete ORF including 1 170 bp which encoded 389 amino acids. Bioinformatic analysis showed that CHS gene included specific peptide sequence RLMMYQQGCFAGGTVLR of chalcone synthase superfamily, but has no signal peptide, belonging to the non-secretory proteins, located inside of cytoplasm. Its molecular evolution is more conservative.[Conclusion] The results above provided foundation for the further studies of structure and function of CHS protein.
基金Project(20A187)supported by the Hunan Provincial Department of Education,ChinaProjects(51408216,51308209)supported by the National Natural Science Foundation of China。
文摘To keep the tunnel face stable is very important for tunnel construction.In this paper,the tunnel face stability under the advanced pipe was analyzed using the Winkler foundation model and rigid limit equilibrium.The tunnel face deformation characteristics were also analyzed using the numerical simulation.The influence of parameters on the deflection of the pipe roof and the stability of the tunnel face were discussed.The results show that the tunnel face stability can be improved through increasing the pipe diameter,decreasing the initial displacement at the beginning of the pipe seat,and adopting the short round length and small excavation height.With the increase of tunnel burial depth,the safety factor of tunnel face stability first decreases,then increases,and then remains unchanged.The deformation at the center of the tunnel face is larger than the deformation at the surround sides and at the corner.The horizontal displacement varies little with the increasing of the pipe length.The horizontal displacement at the center of the tunnel face increases with the increase of the pipe ring spacing and the pipe longitudinal spacing.There is an optimum external angle.
基金Supported by Agricultural Improved Variety Project of Shandong Province(LKZ[2014]No.96)
文摘[Objective] This study aimed to clone and analyze the sequence of CHS gene from Acer truncatum leaves. [Method] Using A. truncatum cultivars No.1-6 as experimental materials, total RNA was extracted from A. truncatum leaves with the modified CTAB method. CHS gene sequences were downloaded from the NCBI and aligned by BLAST. Degenerate primers were designed by DNAMAN and Primer- premier5 to amplify the target band. CHS gene fragment was amplified by RT-PCR and ligated to pMD18-T vector. The identified positive colonies were sequenced. [Result] A 1 365 bp fragment was amplified. Sequence analysis suggested that the obtained fragment encoded 365 amino acids and shared above 90% homology to nucleotide sequence of CHS gene from A. palmatum and A. [Conclusion] In this study, CHS gene was successfully cloned from A. truncatum for the first time, which laid the foundation for efficient utilization of CHS gene.
