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Therapeutic effect of regulating autophagy in spinal cord injury: a network meta-analysis of direct and indirect comparisons 被引量:6
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作者 Duo Zhang Di Zhu +4 位作者 Fang Wang Ji-Chao Zhu Xu Zhai Yuan Yuan Chen-Xi Li 《Neural Regeneration Research》 SCIE CAS CSCD 2020年第6期1120-1132,共13页
Objective:An increasing number of studies indicate that autophagy plays an important role in the pathogenesis of spinal cord injury,and that regulating autophagy can enhance recovery from spinal cord injury.However,th... Objective:An increasing number of studies indicate that autophagy plays an important role in the pathogenesis of spinal cord injury,and that regulating autophagy can enhance recovery from spinal cord injury.However,the effect of regulating autophagy and whether autophagy is detrimental or beneficial after spinal cord injury remain unclear.Therefore,in this study we evaluated the effects of autophagy regulation on spinal cord injury in rats by direct and indirect comparison,in an effort to provide a basis for further research.Data source:Relevant literature published from inception to February 1,2018 were included by searching Wanfang,CNKI,Web of Science,MEDLINE(OvidSP),PubMed and Google Scholar in English and Chinese.The keywords included"autophagy","spinal cord injury",and"rat".Data selection:The literature included in vivo experimental studies on autophagy regulation in the treatment of spinal cord injury(including intervention pre-and post-spinal cord injury).Meta-analyses were conducted at different time points to compare the therapeutic effects of promoting or inhibiting autophagy,and subgroup analyses were also conducted.Outcome measure:Basso,Beattie,and Bresnahan scores.Results:Of the 622 studies,33 studies of median quality were included in the analyses.Basso,Beattie,and Bresnahan scores were higher at 1 day(MD=1.80,95%CI:0.81-2.79,P=0.0004),3 days(MD=0.92,95%CI:0.72-1.13,P<0.00001),1 week(MD=2.39,95%CI:1.85-2.92,P<0.00001),2 weeks(MD=3.26,95%CI:2.40-4.13,P<0.00001),3 weeks(MD=3.13,95%CI:2.51-3.75,P<0.00001)and 4 weeks(MD=3.18,95%CI:2.43-3.92,P<0.00001)after spinal cord injury with upregulation of autophagy compared with the control group(drug solvent control,such as saline group).Basso,Beattie,and Bresnahan scores were higher at 1 day(MD=6.48,95%CI:5.83-7.13,P<0.00001),2 weeks(MD=2.43,95%CI:0.79-4.07,P=0.004),3 weeks(MD=2.96,95%CI:0.09-5.84,P=0.04)and 4 weeks(MD=4.41,95%CI:1.08-7.75,P=0.01)after spinal cord injury with downregulation of autophagy compared with the control group.Indirect comparison of upregulation and downregulation of autophagy showed no differences in Basso,Beattie,and Bresnahan scores at 1 day(MD=-4.68,95%CI:-5.840 to-3.496,P=0.94644),3 days(MD=-0.28,95%CI:-2.231-1.671,P=0.99448),1 week(MD=1.83,95%CI:0.0076-3.584,P=0.94588),2 weeks(MD=0.81,95%CI:-0.850-2.470,P=0.93055),3 weeks(MD=0.17,95%Cl:-2.771-3.111,P=0.99546)or 4 weeks(MD=-1.23,95%Cl:-4.647-2.187,P=0.98264)compared with the control group.Conclusion:Regulation of autophagy improves neurological function,whether it is upregulated or downregulated.There was no difference between upregulation and downregulation of autophagy in the treatment of spinal cord injury.The variability in results among the studies may be associated with differences in research methods,the lack of clearly defined autophagy characteristics after spinal cord injury,and the limited autophagy monitoring techniques.Thus,methods should be standardized,and the dynamic regulation of autophagy should be examined in future studies. 展开更多
关键词 AUTOPHAGY Basso Beattie and Bresnahan SCORES indirect comparison meta-analysis NERVE REGENERATION neural REGENERATION NEUROLOGICAL function rat models regulation spinal CORD injury strategy analysis
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The export status of major rare earth products in China in the first half of 2017
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作者 Liu Xiaofang 《China Rare Earth Information》 2017年第9期1-5,共5页
According to Customs-info network, China totally exported about 26,200 tons of rare earth products during the first half of 2017, valuing $204 million or $7.77 per kg. Export volume rose by 15.32 percent year-on-year ... According to Customs-info network, China totally exported about 26,200 tons of rare earth products during the first half of 2017, valuing $204 million or $7.77 per kg. Export volume rose by 15.32 percent year-on-year but export value dropped down by 20.82 percent. Export of rare earth metals reached 2,760 tons with an export value of $3.42 million or $12.40 per kilogram. The export volume of rare earth compounds was about 23,500 tons, with an export value of $169 million or $7.22 per kg. The rare earth export market has been continuing the trend of prices falling. 展开更多
关键词 According to Customs-info network China totally exportED about 26 200 TONS of RARE earth products during the first HALF of 2017
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Denial of Service Due to Direct and Indirect ARP Storm Attacks in LAN Environment 被引量:2
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作者 Sanjeev Kumar Orifiel Gomez 《Journal of Information Security》 2010年第2期88-94,共7页
ARP-based Distributed Denial of Service (DDoS) attacks due to ARP-storms can happen in local area networks where many computer systems are infected by worms such as Code Red or by DDoS agents. In ARP attack, the DDoS ... ARP-based Distributed Denial of Service (DDoS) attacks due to ARP-storms can happen in local area networks where many computer systems are infected by worms such as Code Red or by DDoS agents. In ARP attack, the DDoS agents constantly send a barrage of ARP requests to the gateway, or to a victim computer within the same sub-network, and tie up the resource of attacked gateway or host. In this paper, we set to measure the impact of ARP-attack on resource exhaustion of computers in a local area network. Based on attack experiments, we measure the exhaustion of processing and memory resources of a victim computer and also other computers, which are located on the same network as the victim computer. Interestingly enough, it is observed that an ARP-attack not only exhausts resource of the victim computer but also significantly exhausts processing resource of other non-victim computers, which happen to be located on the same local area network as the victim computer. 展开更多
关键词 ARP ATTACK COMPUTER Network Security COMPUTER Systems DIRECT ATTACK Distributed DENIAL of Service ATTACKS (DDoS) indirect ATTACK Local Area Networks
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Indirect Adaptive Robust Trajectory Tracking Control of Hard Rock TBM with Load Variation of Tunneling Face 被引量:2
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作者 Chengjun Shao Jianfeng Liao +1 位作者 Zhitao Liu Hongye Su 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2019年第2期168-179,共12页
Posture adjustment of open-type hard rock tunnel boring machine(TBM) can be achieved by properly adjusting the hydraulic pressure of gripper cylinder and torque cylinders. However, the time-varying inhomogeneous load ... Posture adjustment of open-type hard rock tunnel boring machine(TBM) can be achieved by properly adjusting the hydraulic pressure of gripper cylinder and torque cylinders. However, the time-varying inhomogeneous load acting on tunneling face of TBM and complex stratum working condition can cause the trajectory deviation. In this paper,the position and posture rectification kinematics and dynamics models of TBM have been established in order to track the trajectory. Moreover, there are uncertain parameters and uncertain loads from complex working conditions in the dynamic model. An indirect adaptive robust control strategy is applied to achieve precise position and posture trajectory tracking control. Simulation results show when the position deviation only occurs in Y-axis and the current orientation is parallel with the designed axis, the deviation can be corrected by controlling the pressure of gripper cylinder and the actual trajectory meets the designed axis when TBM is pushed forward 0.14 m in X-axis. If the deviation only occurs in Z-axis, then the deviation can be corrected by controlling torque cylinders. If the position deviation occurs both in Y-axis and Z-axis at the same time, the pressure of gripper cylinder and torque cylinders should be controlled at the same time to rectify the deviation. Simulation results are shown to illustrate the e ectiveness and robustness of the proposed controller. This research proposes an indirect adaptive robust controller that can track the planned tracking trajectory smoothly and rapidly. 展开更多
关键词 Open type HARD rock TBM INHOMOGENEOUS load Position and POSTURE RECTIFICATION indirect adaptive robust control
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棉花大丽轮枝菌(Verticillium dahliae)血清学检测——抗原处理的比较 被引量:5
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作者 李亚宁 魏艳敏 +2 位作者 刘大群 杨文香 张汀 《河北农业大学学报》 CAS CSCD 北大核心 2005年第2期79-84,共6页
分别以棉花黄萎病菌V20的菌丝研磨物、菌丝蛋白、超氧化物歧化酶酶带Ⅰ、超氧化物歧化酶酶带Ⅱ为抗原免疫家兔,制备了抗血清As1、As2、As3、As4。琼脂双扩散法(ADD)和间接ELISA方法测定其特异性,发现这4种抗血清既可与同种的抗原发生阳... 分别以棉花黄萎病菌V20的菌丝研磨物、菌丝蛋白、超氧化物歧化酶酶带Ⅰ、超氧化物歧化酶酶带Ⅱ为抗原免疫家兔,制备了抗血清As1、As2、As3、As4。琼脂双扩散法(ADD)和间接ELISA方法测定其特异性,发现这4种抗血清既可与同种的抗原发生阳性反应,也可与不同种的抗原发生交叉反应。用异种抗原黑白轮枝菌和尖孢镰刀菌吸附这4种抗血清,排除其非特异性成分后,抗血清专化性明显提高,消除了交叉反应。特异性测定表明:经吸附后的4种抗血清可以准确地将大丽轮枝菌鉴定到种的水平,但仍不能区分其生理型和致病类型。另外,当分别使用菌丝研磨物或菌丝蛋白为特异性测定抗原时,ADD检测的结果虽然一致,但前者阳性反应沉淀线粗糙,轮廓不清晰,后者的沉淀线较细,且清晰、明显。因此适宜选择菌丝蛋白为抗原进行抗血清的特异性测定试验。 展开更多
关键词 e ELISA V20 ADD
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Detection and Separation of Inorganic Cations in Natural, Potable, and Wastewater Samples Using Capillary Zone Electrophoresis with Indirect UV Detection 被引量:1
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作者 Lara Varden Fadi Bou-Abdallah 《American Journal of Analytical Chemistry》 2017年第1期81-94,共14页
Capillary zone electrophoresis (CZE) is a sensitive and rapid technique for determining traces of inorganic cations in water samples. CZE with indirect UV-diode array detection (CZE-DAD) was utilized to identify sever... Capillary zone electrophoresis (CZE) is a sensitive and rapid technique for determining traces of inorganic cations in water samples. CZE with indirect UV-diode array detection (CZE-DAD) was utilized to identify several inorganic cations in natural, potable, and wastewater samples. A pH 4.35 background electrolyte system was employed and consisted of 15 mM imidazole, 8 mM malonic acid, 2 mM 18-crown-6 ether as complexing agents, 10% v/v methanol as an organic modifier with indirect absorbance reference at 214 nm. The CZE method involved electromigration injection at 5 kV for 5 s, a separation voltage of 20 kV at 25℃, and a detection wavelength of 280 nm. Six main cations (ammonium , potassium K+, calcium Ca2+, sodium Na+, magnesium Mg2+, and lead Pb2+) were tested, and all but lead, were detected in the water samples at concentrations between 0.03 and 755 ppm with a detection limit ranging between 0.023 and 0.084 ppm. The successful evaluation of the proposed methodology allowed us to reliably detect and separate six metal ions in different water samples without any pretreatment. All water samples were collected from Northern New York towns and the Raquette River water system, the third longest river in New York State and the largest watershed of the central and western Adirondacks. 展开更多
关键词 CAPILLARY Zone ELECTROPHORESIS indirect UV DETECTION Inorganic CATIONS Northern New YORK Raquette River Adirondack Watershed
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禽流感病毒NP蛋白单克隆抗体的研制和鉴定 被引量:2
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作者 黄庆华 张果平 王可 《江西农业学报》 CAS 2010年第12期141-143,共3页
应用杂交瘤技术,以低致病性禽流感病毒H9N2亚型(Mildly Pathogenic Avian Influenza Virus,MPAIV)F株作为免疫原,制备出3株稳定分泌特异性单克隆抗体的细胞株C2C5、D3B10和C2H8。对单抗的特异性鉴定结果表明:腹水单抗C2C5、D3B10和C2H8... 应用杂交瘤技术,以低致病性禽流感病毒H9N2亚型(Mildly Pathogenic Avian Influenza Virus,MPAIV)F株作为免疫原,制备出3株稳定分泌特异性单克隆抗体的细胞株C2C5、D3B10和C2H8。对单抗的特异性鉴定结果表明:腹水单抗C2C5、D3B10和C2H8的间接ELISA效价分别为5×214、210、5×212,但均无HI效价。3株单抗的免疫球蛋白亚类均为IgG2a。SDS-PAGE和Western-blot分析结果显示:单抗C2H8和D3B10与AIV H9N2和AIV H5N1均有60 kD的蛋白条带反应,而不与其他条带反应,表明这两株单抗特异性地针对AIV H9和AIV H5的共同抗原成分60 kD NP蛋白。而C2C5不与电泳蛋白条带反应,说明该单抗不是针对线性表位的,而可能是针对空间表位的。 展开更多
关键词 Avian Influenza Virus MONOCLONAL Antibodies WESTERN-BLOT AIV ELISA 线 H9N2
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The Development and Application of an Indirect ELISA Test for the Detection of Chicken Anaemia Virus (CAV) by VP1 in Chicken Flock Serum
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作者 Elham O. Mahgoub 《Open Journal of Genetics》 2014年第4期316-331,共16页
Chicken anaemia virus (CAV) causes a viral disease in chickens worldwide and thus has economic importance. The main aim of this study was to develop a rapid, sensitive and specific VP1-CAVI indirect ELISA for the dete... Chicken anaemia virus (CAV) causes a viral disease in chickens worldwide and thus has economic importance. The main aim of this study was to develop a rapid, sensitive and specific VP1-CAVI indirect ELISA for the detection of CAV infection. The CAV-VP1, was separately cloned and expressed in recombinant E. coli. The purified recombinant CAV-VP1 protein was then coated as an antigen on an ELISA plates to evaluate its reactivity against chicken sera. The resulting indirect ELISA was then compared with a commercial ELISA. The specificity and sensitivity of the indirect ELISA were measured as 93.3% and 100%, respectively. A t-test produced a t-value of 15.805 for the indirect ELISA and revealed a significant difference between CAV-positive serum and CAV-negative serum (p-value of 0.001). For the second variable (i.e., a commercial ELISA), the t-test yielded a t-value of 5.063, which revealed a significant difference between CAV-positive serum and CAV-negative serum (p-value of 0.015). This intervention produces statistically significant improvements in both variables (p-values < 0.05). The correlation coefficient for the indirect ELISA was r = 0.93. Therefore, this work can be considered as a new achievement in diagnosis for Chicken anaemia virus in chicken flocks. 展开更多
关键词 indirect ELISA Commercial ELISA CHICKEN ANAEMIA Virus Recombinant Protein VP1 Sensitivity Specificity Standard Curve for indirect ELISA
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PRRSV间接免疫荧光检测法的建立和应用 被引量:17
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作者 彭长凌 高崧 刘秀梵 《中国兽医科技》 CSCD 北大核心 2005年第4期256-260,共5页
以猪生殖与呼吸综合征病毒(PRRSV) ATCC VR 2332 株人工单独感染3 头3 周龄仔猪,并用PRRSV ATCC VR 2332株和猪圆环病毒2型(PCV2)联合感染2 头3 周龄仔猪,每日检查试验猪体温、采食情况及临床表现,并用HerdChek IDEXX 试剂盒测其血清抗... 以猪生殖与呼吸综合征病毒(PRRSV) ATCC VR 2332 株人工单独感染3 头3 周龄仔猪,并用PRRSV ATCC VR 2332株和猪圆环病毒2型(PCV2)联合感染2 头3 周龄仔猪,每日检查试验猪体温、采食情况及临床表现,并用HerdChek IDEXX 试剂盒测其血清抗体。PRRSV+PCV2联合感染猪分别在感染后1、3周剖杀,PRRSV单独感染猪和健康对照猪分别在感染后1、2和3周剖杀,对全部试验猪的肺组织进行灌洗处理以获得肺泡巨噬细胞(PAM),用PAM进行抹片,同时用肺组织直接进行触片做间接免疫荧光(IIF)检测。结果表明,用所获得的3 株单克隆抗体均检测到人工感染猪肺组织中的PRRSV抗原。另外,用IIF检测7 头临床疑似PRRS病猪的PAM抹片,其中沭阳县的2头猪呈现阳性反应;对这2头猪的肺组织进行病毒分离,在盲传到第4代时Marc 145细胞上产生明显的细胞病变,进一步证实用所获得的3株单克隆抗体检测临床样品的结果准确而可靠,且获得的3株单克隆抗体均可通过IIF用于检测猪肺组织中的PRRSV抗原。 展开更多
关键词 PRRSV 2 ATCC
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Study on the Impacts of Japanese Positive List System on the Chinese Frozen Vegetable Exportation
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作者 Jian Chen Min Xin Ziye Zhou 《Journal of Finance Research》 2017年第1期9-13,共5页
This paper mainly studies the Japanese policy, especially the Positive List System and Health Quarantine System, on the Chinese agricultural products exports, taking the frozen vegetable for example. The authors carry... This paper mainly studies the Japanese policy, especially the Positive List System and Health Quarantine System, on the Chinese agricultural products exports, taking the frozen vegetable for example. The authors carry out studies on the related polices, consider the non-policy factors, making comparative analysis among the three countries: China, the USA and Thailand, utilizing trade gravity model. Finally the authors present suggestions to the breaking of the trade barriers. 展开更多
关键词 Green TRADE barrier JAPANESE positive LIST system FROZEN VEGETABLE export Gravity model Agricultural product TRADE
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从四川长虹巨额坏账看出口信用保险在我国外贸发展中的积极影响 被引量:4
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作者 严复雷 《黑龙江对外经贸》 2005年第4期49-51,共3页
2004年年底四川长虹公告称公司将计提高达27亿人民币的坏账准备,这个巨额数字主要来自于其在美国的主要进口商APEX公司的巨额应收账款可能无法收回,这个重大事件再次给那些只注重销量增长而不注意收汇安全的外贸企业敲了警种。提高外贸... 2004年年底四川长虹公告称公司将计提高达27亿人民币的坏账准备,这个巨额数字主要来自于其在美国的主要进口商APEX公司的巨额应收账款可能无法收回,这个重大事件再次给那些只注重销量增长而不注意收汇安全的外贸企业敲了警种。提高外贸信用风险管理意识,防范和化解对外贸易中的信用风险,保证出口收汇安全,是任何一家外贸企业必须慎重对待的问题。作者以此为例,提出出口信用保险对发展我国外贸有着积极影响。 展开更多
关键词 APEX 2004
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Assessing the Effects of Indirect Left Turn on a Signalized Intersection Performance: A Case Study for the Tehran Metropolitan Area
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作者 Maxim A. Dulebenets Amir M. Rahimi Arash Mazaheri 《Open Journal of Applied Sciences》 2017年第11期617-634,共18页
Many signalized intersections are characterized with frequent left-turn moves. Vehicles waiting for a protected left turn may form long queues, which will increase the intersection delay and negatively impact the netw... Many signalized intersections are characterized with frequent left-turn moves. Vehicles waiting for a protected left turn may form long queues, which will increase the intersection delay and negatively impact the network performance. Researchers and practitioners across various countries underline that access management leads to a smoother traffic flow. One way of access management at intersections is to eliminate the direct left-turn maneuver. This study aims to evaluate how the traffic conditions will be affected from replacing the direct left turn with the right-turn U-turn maneuver at intersections. In case of the right-turn U-turn maneuver, a vehicle turns right instead of making the left turn and travels either to the median opening or to the next intersection to make a U-turn. Two simulation models are built using the Synchro Studio and Aimsun simulation software packages based on the data, collected from one of the busiest intersections in Tehran (Iran), to quantify the effects of replacing the direct left turn with the right-turn U-turn maneuver on the intersection and network performance. Results of a comprehensive simulation analysis indicate that the proposed access management treatment not only significantly reduces the total vehicle queue length and the total delay at the considered intersection, but also decreases the total network delay and the total travel time. Furthermore, elimination of the direct left turn increases the number of vehicles entering the network. 展开更多
关键词 Transportation Engineering Signalized INTERSECTION indirect LEFT TURN Access Management Traffic Simulation SYNCHRO Aimsun
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China's textile and garment exports showed a decline in the first two months of 2025
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《China Textile》 2025年第2期30-33,共4页
In January and February of 2025,China's textile and clothing exports totaled 42.88 billion US dollars,marking a 4.5%decrease compared to the same period in the previous year.The primary factors contributing to thi... In January and February of 2025,China's textile and clothing exports totaled 42.88 billion US dollars,marking a 4.5%decrease compared to the same period in the previous year.The primary factors contributing to this decline are as follows:First,China’s textile and clothing exports nearly reached USD 45 billion in January and February of 2024,setting a high base.Second,in order to avoid tariffs imposed by the United States,enterprises generally concentrated their exports at the end of last year and January this year. 展开更多
关键词 GARMENT exportS textile clothing exports DECLINE TEXTILE TARIFFS China United States
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A Direct Trust dependent Link State Routing Protocol Using Route Trusts for WSNs (DTLSRP)
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作者 Shaik Sahil Babu Arnab Raha Mrinal Kanti Naskar 《Wireless Sensor Network》 2011年第4期125-134,共10页
The traditional cryptographic security techniques are not sufficient for secure routing of message from source to destination in Wireless Sensor Networks (WSNs), because it requires sophisticated software, hardware, l... The traditional cryptographic security techniques are not sufficient for secure routing of message from source to destination in Wireless Sensor Networks (WSNs), because it requires sophisticated software, hardware, large memory, high processing speed and communication bandwidth. It is not economic and feasible because, depending on the application, WSN nodes are high-volume in number (hence, limited resources at each node), deployment area may be hazardous, unattended and/or hostile and sometimes dangerous. As WSNs are characterized by severely constrained resources and requirement to operate in an ad-hoc manner, security functionality implementation to protect nodes from adversary forces and secure routing of message from source node to base station has become a challenging task. In this paper, we present a direct trust dependent link state routing using route trusts which protects WSNs against routing attacks by eliminating the un-trusted nodes before making routes and finding best trustworthy route among them. We compare our work with the most prevalent routing protocols and show its benefits over them. 展开更多
关键词 Wireless Sensor Network (WSN) Geometric Mean (GM) Direct TRUST indirect TRUST ROUTE TRUST (RT) Base Station (BS) Benevolent NODE MALICIOUS NODE Homogeneous Nodes PACKET Latency PACKET Transmission Rate MATLAB
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Chinese clothing exports bloom with resilience amidst challenges
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作者 Qiu Shuchen 《China Textile》 2025年第4期32-33,共2页
Since the beginning of this year, the China-US economic and trade tensions have brought many challenges to China's clothing export companies. Nevertheless, in the face of difficulties, China's clothing foreign... Since the beginning of this year, the China-US economic and trade tensions have brought many challenges to China's clothing export companies. Nevertheless, in the face of difficulties, China's clothing foreign trade still shows strong resilience and adaptability. From January to May, China's clothing exports showed characteristics such as increased volume, lower prices, category differentiation, and multi-point market layout. Meanwhile, Chinese clothing companies have actively responded to trade friction impacts by closely monitoring tariff negotiations, accelerating transformation and upgrading, exploring diversified international markets, and deepening domestic markets. 展开更多
关键词 increased volume clothing export companies RESILIENCE strong resilience China US economic trade tensions Chinese clothing exports lower prices adaptability
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盐城地区出国劳务人群梅毒感染状况及防制对策 被引量:1
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作者 杨志俊 徐素荣 《口岸卫生控制》 2005年第2期31-33,共3页
目的根据盐城地区出国劳务人员传染病监测结果,探讨梅毒的防制对策。方法使用上海荣盛公司提供的TRUST诊断试剂初筛,日本富士公司TPPA试剂或ELISA试剂确认,技术操作及结果判断标准依据该试剂盒所附说明书,在有效期内使用,并结合流行病... 目的根据盐城地区出国劳务人员传染病监测结果,探讨梅毒的防制对策。方法使用上海荣盛公司提供的TRUST诊断试剂初筛,日本富士公司TPPA试剂或ELISA试剂确认,技术操作及结果判断标准依据该试剂盒所附说明书,在有效期内使用,并结合流行病学调查。结果劳务体检一直占该局体检量的90%以上,性传播疾病(STD)是监测的重点疫病,梅毒又是近几年来发现的最主要的病种,共发现劳务人员中阳性感染者48人,占该局发现疫病总数的82.36%,隐性梅毒占91.2%,20-39岁占93.75%;男性阳性率为3.37‰,女性为2.93‰,混合感染率3.17‰;低滴度感染者为主,1:2-1:4占81.25‰。结论出国劳务是盐城地区的优势产业,应加强对出国劳务人员性传播疾病的检疫与监测工作,保证劳务输出人员无疫病出国,构筑严密的检疫体系,进行全面系统监测,加强防治常识的宣传教育,严禁卖淫、嫖娼以及积极治疗是梅毒病人预防的根本措施。 展开更多
关键词 (STD) ELISA TPPA TRUST
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汉坦病毒G2糖蛋白在昆虫细胞中的表达及其在免疫诊断中的应用 被引量:1
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作者 朱国献 朱函坪 +2 位作者 梁伟峰 姚苹苹 朱智勇 《中国媒介生物学及控制杂志》 CAS CSCD 北大核心 2005年第3期199-201,共3页
目的构建汉坦病毒(HV)Z10株包膜糖蛋白G2基因的重组表达载体,探索其在昆虫细胞中的表达及在免疫荧光诊断中的应用。方法以质粒pUCmZ10M为模板设计引物,用聚合酶链反应(PCR)扩增Z10株G2基因片段,将G2片段克隆入pUCmT质粒载体,碱法提取质... 目的构建汉坦病毒(HV)Z10株包膜糖蛋白G2基因的重组表达载体,探索其在昆虫细胞中的表达及在免疫荧光诊断中的应用。方法以质粒pUCmZ10M为模板设计引物,用聚合酶链反应(PCR)扩增Z10株G2基因片段,将G2片段克隆入pUCmT质粒载体,碱法提取质粒pUCmZ10G2,酶切后将回收片段插入杆状病毒转移载体pFastBacHTa,构建重组体pFastBacHTaZ10G2,重组体转化感受态细胞E.coliDH10Bac后,经2轮筛选,提取重组质粒转染昆虫细胞sf9,并用间接免疫荧光技术检测表达的G2蛋白。结果获得含有编码HVZ10株的包膜糖蛋白G2基因的重组质粒,转染昆虫细胞表达出G2蛋白,与肾综合征出血热(HFRS)阳性血清反应,昆虫细胞内呈现特异性环状荧光。检测40份HFRS血清,与全病毒细胞抗原片的符合率为95%。结论成功构建HVZ10株包膜糖蛋白G2基因的表达载体,并在昆虫细胞中表达。可利用重组G2蛋白检测HV感染。 展开更多
关键词 G2 E.coli
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Swiss textile machinery exports jump 12.2% in H1 2025,amidst trade headwinds Interview with Ms Cornelia Buchwalder,Secretary General,Swiss Textile Machinery
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《China Textile》 2025年第5期20-21,共2页
The Swiss textile machinery industry has staged a strong recovery in the first half of 2025,with export volumes rising by 12.2%compared to the same period last year.This positive rebound marks a significant turnaround... The Swiss textile machinery industry has staged a strong recovery in the first half of 2025,with export volumes rising by 12.2%compared to the same period last year.This positive rebound marks a significant turnaround from a challenging 2024,which saw exports decline by 17.7%.The upturn is fueled by renewed demand from key Asian markets,including China,India,and Bangladesh,alongside growing interest from Southeast Asia and North Africa.This resurgence highlights the resilience of Swiss manufacturers and the global industry's ongoing drive toward advanced and sustainable technologies,even amidst complex global economic conditions. 展开更多
关键词 exportS textile machinery Asian markets India Bangladesh recovery China trade headwinds
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Setting Standards From manufacturing equipment to exporting standards,China is powering the world with clean energy
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作者 GE LIJUN 《ChinAfrica》 2025年第11期51-53,共3页
China’s Dongfang Electric Machinery successfully completed the manufacturing of the first batch of embedded components for six turbines it is supplying for the Koysha Hydroelectric Dam project in Ethiopia.Transported... China’s Dongfang Electric Machinery successfully completed the manufacturing of the first batch of embedded components for six turbines it is supplying for the Koysha Hydroelectric Dam project in Ethiopia.Transported by land and sea,these parts will arrive on site in three months.Once completed,the power plant will generate more than 6 billion kwh of electricity annually,reducing carbon dioxide emissions by 1 million tonnes per year. 展开更多
关键词 carbon dioxide exporting standards hydroelectric dam carbon dioxide emissions power plant clean energy manufacturing equipment embedded components
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Port of Prosperity--Ghana’s port expansion key to realising the country’s vision to establish an export-driven economic model
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作者 LI YIN 《ChinAfrica》 2025年第6期22-23,共2页
Ghana’s Tema Port marked a historic milestone on 16 January 2024 with the arrival of Maersk Edirne,a 13,676-TEU container vessel-one of the largest of its kind globally-as part of Maersk’s newly launched Far East-We... Ghana’s Tema Port marked a historic milestone on 16 January 2024 with the arrival of Maersk Edirne,a 13,676-TEU container vessel-one of the largest of its kind globally-as part of Maersk’s newly launched Far East-West Africa express service. 展开更多
关键词 Maersk Edirne port expansion Tema Port Far East West Africa Express Service Ghana container vessel maersk edirnea export driven economy
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