The level of urinary albumin is a critical indicator for the early diagnosis and management of chronic kidney disease(CKD).However,existing methods for detecting albumin are not conducive to point-of-care testing due ...The level of urinary albumin is a critical indicator for the early diagnosis and management of chronic kidney disease(CKD).However,existing methods for detecting albumin are not conducive to point-of-care testing due to the complexity of reagent addition and incubation processes.This study presents a smartphone-integrated handheld automated biochemical analyzer(sHABA)designed for point-of-care testing of urinary albumin.The sHABA features a pre-loaded,disposable reagent cassette with reagents for the albumin assay arranged in the order of their addition within a hose.The smartphone-integrated analyzer can drive the reagents following a preset program,to enable automatic sequential addition.The sHABA has a detection limit for albumin of 5.9 mg/L and a linear detection range from 7 to 450 mg/L.The consistency of albumin level detection in 931 urine samples using sHABA with clinical tests indicates good sensitivity(95.78%)and specificity(90.16%).This research advances the field by providing an automated detection method for albumin in a portable device,allowing even untrained individuals to monitor CKD in real time at the patient's bedside.In the context of promoting tiered diagnosis and treatment,the sHABA has the potential to become an essential tool for the early diagnosis and comprehensive management of CKD and other chronic conditions.展开更多
Biomechanical study of the visual system by ocular response analyzer investigates the inter-structural biological relationships,mechanics,and function of the visual system.This review aimed to investigate the changes ...Biomechanical study of the visual system by ocular response analyzer investigates the inter-structural biological relationships,mechanics,and function of the visual system.This review aimed to investigate the changes in corneal biomechanical parameters with age and sex.The articles published in PubMed between 2000 and 2021 were investigated and critiqued,and valid scientific evidence was collected,reviewed and concluded according to the inclusion and exclusion criteria.Most studies showed that corneal biomechanical changes occur infrequently in children up to the age of 20y,and with increasing age and wider age range,there was a significant decrease in corneal biomechanical indices,especially corneal hysteresis.In children and adults,most studies have shown that these biomechanical indicators,especially corneal resistance factor,were higher in females.Although hormonal changes may contribute to this finding,the role of axial length and other biometric indicators should not be ignored.The axial length,the intraocular pressure,and the corneal thickness are other factors associated with biomechanical parameters that should be taken into account in clinical diagnosis and management especially for patients undergoing refractive surgery as well as keratoconus patients.展开更多
Air-sea water vapor and CO_(2) flux observation experiments were carried out at the Yantai National Satellite Ocean Calibration Platform and the jetty at Monolithic Beach,Juehua Island,using a 100 Hz gas analyzer.The ...Air-sea water vapor and CO_(2) flux observation experiments were carried out at the Yantai National Satellite Ocean Calibration Platform and the jetty at Monolithic Beach,Juehua Island,using a 100 Hz gas analyzer.The observations were corrected by employing wild point rejection,linear detrending,delay correction,coordinate rotation,time matching,and Webb,Pearman,and Leuning(WPL)correction.The results of spectral analysis and a turbulence development adequacy data quality check showed that the overall observation data quality was good.The air-sea water vapor and CO_(2) flux results showed that the observation duration affected both the air-sea flux intensity and direction at different observation frequencies.At shorter observation durations,the air-sea flux values measured at 100 Hz were smaller than the 20 Hz measurements and had opposite directions.In addition,the WPL correction reduced the overall air-sea flux and partially minimized the effect of observation frequency on the air-sea flux intensity.These results showed that high-frequency observations showed more turbulence variations than low-frequency observations.This conclusion could promote an understanding of small-scale turbulence variations.展开更多
An E//B neutral particle analyzer(NPA)has been designed and is under development at Sichuan University and Southwestern Institute of Physics.The main purpose of the E//B NPA is to measure the distribution function of ...An E//B neutral particle analyzer(NPA)has been designed and is under development at Sichuan University and Southwestern Institute of Physics.The main purpose of the E//B NPA is to measure the distribution function of fast ions in the HL-2A/3 tokamak.The E//B NPA contains three main units,i.e.the stripping unit,the analyzing unit and the detection unit.A gas stripping chamber was adopted as the stripping unit.The results of the simulations and beam tests for the stripping chamber are presented.Parallel electric and magnetic fields provided by a NdFeB permanent magnet and two parallel electric plates were designed and constructed for the analyzing unit.The calibration of the magnetic and electric fields was performed using a 50 kV electron cyclotron resonance ion source(ECRIS)platform.The detection unit consists of 32lutetium-yttrium oxyorthosilicate(LYSO)detector modules arranged in two rows.The response functions ofα,hydrogen ions(H^(+),H_(2)^(+)and H_(3)^(+))andγfor a detector module were measured with^(241)Am,^(137)Cs and^(152)Eu sources together with the 50 kV ECRIS platform.The overall results indicate that the designed E//B NPA device is capable of measuring the intensity of neutral hydrogen and deuteron atoms with energy higher than 20 keV.展开更多
Left-handedness with three zero-absorption windows is achieved in a triple-quantum-dot system. With the typ- ical parameters of a GaAs/AlGaAs heterostructure, the simultaneous negative relative electric permittivity a...Left-handedness with three zero-absorption windows is achieved in a triple-quantum-dot system. With the typ- ical parameters of a GaAs/AlGaAs heterostructure, the simultaneous negative relative electric permittivity and magnetic permeability are obtained by the adjustable incoherent pumping field and two inter-dot tunnelings. Furthermore, three zero-absorption windows in the left-handedness frequency bands are observed. The left- handedness with zero-absorption in the solid state heterostrueture may solve the challenges not only in the left-handed materials achieved by the photonic resonant scheme but also in the application of negative refractive materials with a large amount of absorption.展开更多
To verify the effectiveness of digital optical 3D image analyzer EvaSKIN in the objective and quantitative evaluation of wrinkles.A total of 115 subjects were recruited,the facial images of the subjects were collected...To verify the effectiveness of digital optical 3D image analyzer EvaSKIN in the objective and quantitative evaluation of wrinkles.A total of 115 subjects were recruited,the facial images of the subjects were collected by digital optical 3D image analyzer and manual camera,the changes of crow’s feet with age were analyzed.Pictures obtained by manual photography can be directly used for observation and preliminary grading of wrinkles.However,the requirements for evaluators are high,and the results are prone to errors,which will affect the accuracy of the evaluation.Therefore,skilled raters are needed.Compared with the manual photography method,the digital optical 3D image analyzer EvaSKIN can realize three-dimensional extraction of wrinkles,and obtain the change trend of crow’s feet with age.20~30 years old,wrinkles begin to appear slowly;wrinkles will increase rapidly at the age of 30~50;The length of 50~60 year old wrinkles is basically fixed,the wrinkles develop longitudewise,gradually widen and deepen,and the area,depth and volume increase is obvious,and the skin aging condition is intensified.the digital optical 3D image analyzer EvaSKIN realizes the 3D extraction of wrinkles,quantifies the circumference,area,average depth,maximum depth and volume of wrinkles,realizes the objective and quantitative evaluation of wrinkle state,is more accurate in the measurement of wrinkles,and provides a new instrument and method for the evaluation of wrinkles.it is a perfect and supplement to the traditional evaluation methods,and to a certain extent,it helps the research and development and evaluation institutions of cosmetics to obtain more abundant and three-dimensional data support.展开更多
AIM:To invastigate intra-and interobserver reliability of interferometry,tear meniscus height(TMH)measurement and meibography(MBG)of an ocular surface analyzer,LacryDiag(Quantel Medical,France).METHODS:Five consecutiv...AIM:To invastigate intra-and interobserver reliability of interferometry,tear meniscus height(TMH)measurement and meibography(MBG)of an ocular surface analyzer,LacryDiag(Quantel Medical,France).METHODS:Five consecutive measurements and subsequent analysis of interferometry,TMH,and MBG were recorded by two examiners using the LacryDiag.To assess intra-and interobserver reliability,we used Cohen’s kappa for categorical variables(interferometry),or intraclass correlation coefficient for continuous variables(TMH,MBG).RESULTS:Thirty eyes of 30 examinees were included.For both observers,there was excellent intraobserver reliability for MBG(0.955 and 0.970 for observer 1 and 2,respectively).Intraobserver reliability for observer 1 was substantial for interferometry(0.799),and excellent for TMH(0.863).Reliability for observer 2 was moderate for interferometry(0.535)and fair to good for TMH(0.431).Interobserver reliability was poor for interferometry(0.074)and fair to good for TMH(0.680)and MBG(0.414).CONCLUSION:LacryDiag ocular surface analyzer in our study proves to be a reliable noninvasive tool for the evaluation of TMH and MBG.As for interferometry,poor interobserver reliability,fair to good intraobserver reliability for observer 1,and moderate for observer 2,leave room for improvement.展开更多
Objective:To investigate the hook effect in the detection of beta2-microglobulin(β2-MG)with different reagents as well as on two fully automated biochemical analyzers and their solutions.Methods:Using immunoturbidime...Objective:To investigate the hook effect in the detection of beta2-microglobulin(β2-MG)with different reagents as well as on two fully automated biochemical analyzers and their solutions.Methods:Using immunoturbidimetric assay forβ2-MG as the research object,β2-MG levels were detected by different concentration gradients ofβ2-microglobulin samples in Liedemann,Mack,and Myriad reagents of three manufacturers on two automatic biochemical analyzers,and the difference of the hook effect was comparatively analyzed.Results:Leadman reagent showed the hook effect on the Beckman AU5800 automated biochemistry analyzer,while both Maccura and Mindray reagents did not show the hook effect.After the experiments,we found the limit value of the pre-zone check of Leadman reagent,and changed the parameters of the instrument,when the limit value of the pre-zone check was reached,the instrument automatically diluted the specimen five times and then detected it again.After changing the parameters of the instrument,the correlation between the three methods of detecting samples of different concentrations was r>0.99.Conclusion:Before selecting the application of immunoturbidimetric reagents,we have to carry out the risk assessment of the hook effect and selectively set the parameters of the pre-zone check based on the highest concentration that may occur in the clinic;for the items that may have the hook effect,we have to selectively set the parameters of the pre-zone check when the ratio of average reaction rate and the immediate reaction rate is at the limit value,and the phenomenon of antigen excess may occur,the instrument will carry out automatic dilution before detection,so as to avoid the issuance of erroneous results of high-value samples due to the hook effect.展开更多
背景由于其强大的语言处理能力和广泛的应用潜力,以ChatGPT为代表的大语言模型引领了医疗领域自然语言处理的新趋势。目的本研究通过文献计量分析揭示2017年以来医疗大语言模型的研究热点、主题分布及未来发展方向。方法通过Web of Scie...背景由于其强大的语言处理能力和广泛的应用潜力,以ChatGPT为代表的大语言模型引领了医疗领域自然语言处理的新趋势。目的本研究通过文献计量分析揭示2017年以来医疗大语言模型的研究热点、主题分布及未来发展方向。方法通过Web of Science、中国知网、万方数据知识服务平台和维普网数据库,系统检索和筛选2017年1月-2024年6月关于医疗大语言模型的文献。利用CiteSpace软件提取文献中的主题关键词等信息,分析并对比国内外研究的演进、热点和趋势。结果共纳入1071篇相关文献,结果显示国外研究集中于人工智能、大语言模型、深度学习、知识图谱等技术在医学中的应用,而国内研究则相对较少,侧重于中文医学问答系统构建和医疗数据非结构化问题处理。结论深化医疗数据挖掘,拓展多场景应用,并借鉴国际大语言模型的微调和应用评估经验,促进我国医疗大语言模型技术的发展和医学领域应用。展开更多
基金funding by the China Postdoctoral Science Foundation(Grant No.:2021M701628)the National Natural Science Foundation of China(Grant No.:82202625)+3 种基金Science and Technology Projects in Guangzhou,China(Grant No.:SL2022A04J02086)President Foundation of Nanfang Hospital,Southern Medical University,China(Grant Nos.:2021B012,and 2021C050)National Science Fund for Distinguished Young Scholars,China(Grant No.:82025024)Key project of the National Natural Science Foundation of China(Grant No.:82230080).
文摘The level of urinary albumin is a critical indicator for the early diagnosis and management of chronic kidney disease(CKD).However,existing methods for detecting albumin are not conducive to point-of-care testing due to the complexity of reagent addition and incubation processes.This study presents a smartphone-integrated handheld automated biochemical analyzer(sHABA)designed for point-of-care testing of urinary albumin.The sHABA features a pre-loaded,disposable reagent cassette with reagents for the albumin assay arranged in the order of their addition within a hose.The smartphone-integrated analyzer can drive the reagents following a preset program,to enable automatic sequential addition.The sHABA has a detection limit for albumin of 5.9 mg/L and a linear detection range from 7 to 450 mg/L.The consistency of albumin level detection in 931 urine samples using sHABA with clinical tests indicates good sensitivity(95.78%)and specificity(90.16%).This research advances the field by providing an automated detection method for albumin in a portable device,allowing even untrained individuals to monitor CKD in real time at the patient's bedside.In the context of promoting tiered diagnosis and treatment,the sHABA has the potential to become an essential tool for the early diagnosis and comprehensive management of CKD and other chronic conditions.
基金Supported by Noor Ophthalmology Research Center.
文摘Biomechanical study of the visual system by ocular response analyzer investigates the inter-structural biological relationships,mechanics,and function of the visual system.This review aimed to investigate the changes in corneal biomechanical parameters with age and sex.The articles published in PubMed between 2000 and 2021 were investigated and critiqued,and valid scientific evidence was collected,reviewed and concluded according to the inclusion and exclusion criteria.Most studies showed that corneal biomechanical changes occur infrequently in children up to the age of 20y,and with increasing age and wider age range,there was a significant decrease in corneal biomechanical indices,especially corneal hysteresis.In children and adults,most studies have shown that these biomechanical indicators,especially corneal resistance factor,were higher in females.Although hormonal changes may contribute to this finding,the role of axial length and other biometric indicators should not be ignored.The axial length,the intraocular pressure,and the corneal thickness are other factors associated with biomechanical parameters that should be taken into account in clinical diagnosis and management especially for patients undergoing refractive surgery as well as keratoconus patients.
基金The National Key Research and Development Program of China under contract Nos 2022YFC3104203 and 2018YFC0213103the Science Foundation of Donghai Laboratory under contract No.DH-2022KF01019+1 种基金the National Natural Science Foundation under contract No.419061522023 Shanghai Education Science Research Project under contract No.C2023120.
文摘Air-sea water vapor and CO_(2) flux observation experiments were carried out at the Yantai National Satellite Ocean Calibration Platform and the jetty at Monolithic Beach,Juehua Island,using a 100 Hz gas analyzer.The observations were corrected by employing wild point rejection,linear detrending,delay correction,coordinate rotation,time matching,and Webb,Pearman,and Leuning(WPL)correction.The results of spectral analysis and a turbulence development adequacy data quality check showed that the overall observation data quality was good.The air-sea water vapor and CO_(2) flux results showed that the observation duration affected both the air-sea flux intensity and direction at different observation frequencies.At shorter observation durations,the air-sea flux values measured at 100 Hz were smaller than the 20 Hz measurements and had opposite directions.In addition,the WPL correction reduced the overall air-sea flux and partially minimized the effect of observation frequency on the air-sea flux intensity.These results showed that high-frequency observations showed more turbulence variations than low-frequency observations.This conclusion could promote an understanding of small-scale turbulence variations.
基金supported by the National Magnetic Confinement Fusion Energy R&D Program of China(No.2018YFE0310200)National Natural Science Foundation of China(Nos.11705242,11805138 and 12175156)the Fundamental Research Funds for the Central Universities in China(Nos.YJ201820 and YJ201954)。
文摘An E//B neutral particle analyzer(NPA)has been designed and is under development at Sichuan University and Southwestern Institute of Physics.The main purpose of the E//B NPA is to measure the distribution function of fast ions in the HL-2A/3 tokamak.The E//B NPA contains three main units,i.e.the stripping unit,the analyzing unit and the detection unit.A gas stripping chamber was adopted as the stripping unit.The results of the simulations and beam tests for the stripping chamber are presented.Parallel electric and magnetic fields provided by a NdFeB permanent magnet and two parallel electric plates were designed and constructed for the analyzing unit.The calibration of the magnetic and electric fields was performed using a 50 kV electron cyclotron resonance ion source(ECRIS)platform.The detection unit consists of 32lutetium-yttrium oxyorthosilicate(LYSO)detector modules arranged in two rows.The response functions ofα,hydrogen ions(H^(+),H_(2)^(+)and H_(3)^(+))andγfor a detector module were measured with^(241)Am,^(137)Cs and^(152)Eu sources together with the 50 kV ECRIS platform.The overall results indicate that the designed E//B NPA device is capable of measuring the intensity of neutral hydrogen and deuteron atoms with energy higher than 20 keV.
基金Supported by the National Natural Science Foundation of China under Grant No 61205205the Foundation for Personnel Training Projects of Yunnan Province under Grant No KKSY201207068
文摘Left-handedness with three zero-absorption windows is achieved in a triple-quantum-dot system. With the typ- ical parameters of a GaAs/AlGaAs heterostructure, the simultaneous negative relative electric permittivity and magnetic permeability are obtained by the adjustable incoherent pumping field and two inter-dot tunnelings. Furthermore, three zero-absorption windows in the left-handedness frequency bands are observed. The left- handedness with zero-absorption in the solid state heterostrueture may solve the challenges not only in the left-handed materials achieved by the photonic resonant scheme but also in the application of negative refractive materials with a large amount of absorption.
文摘To verify the effectiveness of digital optical 3D image analyzer EvaSKIN in the objective and quantitative evaluation of wrinkles.A total of 115 subjects were recruited,the facial images of the subjects were collected by digital optical 3D image analyzer and manual camera,the changes of crow’s feet with age were analyzed.Pictures obtained by manual photography can be directly used for observation and preliminary grading of wrinkles.However,the requirements for evaluators are high,and the results are prone to errors,which will affect the accuracy of the evaluation.Therefore,skilled raters are needed.Compared with the manual photography method,the digital optical 3D image analyzer EvaSKIN can realize three-dimensional extraction of wrinkles,and obtain the change trend of crow’s feet with age.20~30 years old,wrinkles begin to appear slowly;wrinkles will increase rapidly at the age of 30~50;The length of 50~60 year old wrinkles is basically fixed,the wrinkles develop longitudewise,gradually widen and deepen,and the area,depth and volume increase is obvious,and the skin aging condition is intensified.the digital optical 3D image analyzer EvaSKIN realizes the 3D extraction of wrinkles,quantifies the circumference,area,average depth,maximum depth and volume of wrinkles,realizes the objective and quantitative evaluation of wrinkle state,is more accurate in the measurement of wrinkles,and provides a new instrument and method for the evaluation of wrinkles.it is a perfect and supplement to the traditional evaluation methods,and to a certain extent,it helps the research and development and evaluation institutions of cosmetics to obtain more abundant and three-dimensional data support.
文摘AIM:To invastigate intra-and interobserver reliability of interferometry,tear meniscus height(TMH)measurement and meibography(MBG)of an ocular surface analyzer,LacryDiag(Quantel Medical,France).METHODS:Five consecutive measurements and subsequent analysis of interferometry,TMH,and MBG were recorded by two examiners using the LacryDiag.To assess intra-and interobserver reliability,we used Cohen’s kappa for categorical variables(interferometry),or intraclass correlation coefficient for continuous variables(TMH,MBG).RESULTS:Thirty eyes of 30 examinees were included.For both observers,there was excellent intraobserver reliability for MBG(0.955 and 0.970 for observer 1 and 2,respectively).Intraobserver reliability for observer 1 was substantial for interferometry(0.799),and excellent for TMH(0.863).Reliability for observer 2 was moderate for interferometry(0.535)and fair to good for TMH(0.431).Interobserver reliability was poor for interferometry(0.074)and fair to good for TMH(0.680)and MBG(0.414).CONCLUSION:LacryDiag ocular surface analyzer in our study proves to be a reliable noninvasive tool for the evaluation of TMH and MBG.As for interferometry,poor interobserver reliability,fair to good intraobserver reliability for observer 1,and moderate for observer 2,leave room for improvement.
基金Baoding Science and Technology Bureau(2141ZF313)。
文摘Objective:To investigate the hook effect in the detection of beta2-microglobulin(β2-MG)with different reagents as well as on two fully automated biochemical analyzers and their solutions.Methods:Using immunoturbidimetric assay forβ2-MG as the research object,β2-MG levels were detected by different concentration gradients ofβ2-microglobulin samples in Liedemann,Mack,and Myriad reagents of three manufacturers on two automatic biochemical analyzers,and the difference of the hook effect was comparatively analyzed.Results:Leadman reagent showed the hook effect on the Beckman AU5800 automated biochemistry analyzer,while both Maccura and Mindray reagents did not show the hook effect.After the experiments,we found the limit value of the pre-zone check of Leadman reagent,and changed the parameters of the instrument,when the limit value of the pre-zone check was reached,the instrument automatically diluted the specimen five times and then detected it again.After changing the parameters of the instrument,the correlation between the three methods of detecting samples of different concentrations was r>0.99.Conclusion:Before selecting the application of immunoturbidimetric reagents,we have to carry out the risk assessment of the hook effect and selectively set the parameters of the pre-zone check based on the highest concentration that may occur in the clinic;for the items that may have the hook effect,we have to selectively set the parameters of the pre-zone check when the ratio of average reaction rate and the immediate reaction rate is at the limit value,and the phenomenon of antigen excess may occur,the instrument will carry out automatic dilution before detection,so as to avoid the issuance of erroneous results of high-value samples due to the hook effect.
文摘背景由于其强大的语言处理能力和广泛的应用潜力,以ChatGPT为代表的大语言模型引领了医疗领域自然语言处理的新趋势。目的本研究通过文献计量分析揭示2017年以来医疗大语言模型的研究热点、主题分布及未来发展方向。方法通过Web of Science、中国知网、万方数据知识服务平台和维普网数据库,系统检索和筛选2017年1月-2024年6月关于医疗大语言模型的文献。利用CiteSpace软件提取文献中的主题关键词等信息,分析并对比国内外研究的演进、热点和趋势。结果共纳入1071篇相关文献,结果显示国外研究集中于人工智能、大语言模型、深度学习、知识图谱等技术在医学中的应用,而国内研究则相对较少,侧重于中文医学问答系统构建和医疗数据非结构化问题处理。结论深化医疗数据挖掘,拓展多场景应用,并借鉴国际大语言模型的微调和应用评估经验,促进我国医疗大语言模型技术的发展和医学领域应用。
