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Analysis and Modelling of Salt-related Deformation in the Western Sichuan Foreland Basin,China
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作者 QIU Dengfeng HU Zongquan +2 位作者 John J.WALSH GUO Jinrui ZHANG Zhongming 《Acta Geologica Sinica(English Edition)》 2026年第1期265-285,共21页
The Western Sichuan Foreland Basin(WSFB)in South China,a prolific hydrocarbon province,exhibits complex structural deformation influenced by Triassic salt tectonics.This paper integrates seismic data and well data to ... The Western Sichuan Foreland Basin(WSFB)in South China,a prolific hydrocarbon province,exhibits complex structural deformation influenced by Triassic salt tectonics.This paper integrates seismic data and well data to elucidate the role of Middle-Lower Triassic evaporite layers in shaping basin structures,focusing on Xinchang Tectonic Zone(XTZ).Salt layers facilitated decoupled deformation between supra-and sub-salt sequences,forming salt pillows and fault-related folds.Three distinct structural trends were identified in XTZ.Key findings reveal that salt thickness variations correlate with deformation styles:thicker salt promoted detachment folding,while thinner salt led to hard-linked fault systems.Sub-salt E-NE trending reverse faults formed horsetail terminations associated with the Pengzhou faults(PzF),deviating from the primary Longmenshan thrust belt(LmsTB)orientation.Structural evolution occurred in three stages:(1)Indosinian salt deposition and foreland basin initiation;(2)Yanshanian eastward propagation of thrust systems with salt-driven detachment folding;(3)Himalayan reactivation overprinting earlier structures with sub-NS trending folds.This work establishes a direct link between salt layers and structural traps,demonstrating how salt acted as a critical detachment layer during multi-stage compression.Results provide insights into the gas exploration of the Late Triassic Xujiahe Formation,emphasizing the importance of salt-influenced deformation in foreland basin systems. 展开更多
关键词 salt structure FAULT horsetail terminations foreland basin western Sichuan
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Mechanisms of ENSO's cross-seasonal modulation of winter–spring atmospheric river activity over East Asia
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作者 Yuliang Zhou Wentao Jia +1 位作者 Weimin Zhang Huizan Wang 《Atmospheric and Oceanic Science Letters》 2026年第1期66-71,共6页
In this study,based on MERRA-2 reanalysis data and a multi-algorithm integrated atmospheric river(AR)iden-tification method,the authors reveal the cross-seasonal regulation mechanism of El Niño-Southern Oscillati... In this study,based on MERRA-2 reanalysis data and a multi-algorithm integrated atmospheric river(AR)iden-tification method,the authors reveal the cross-seasonal regulation mechanism of El Niño-Southern Oscillation(ENSO)on winter-spring AR activities in East Asia.The results show that ENSO asymmetrically modulates AR ac-tivity through teleconnection and hysteresis effects,and has significant enhancement/inhibition effects on ARs in different regions.At the onset of El Niño,enhanced southwesterly flow at the western edge of the western Pacific subtropical high(WPSH)leads to enhanced AR activity in the western Pacific,and anomalous southerly winds in the Indian Ocean promote northward transport of water vapor in the Arabian Sea and Bay of Bengal.With a three-month lag,the weakening and eastward retreat of the WPSH weakens the low-latitude AR activity,but persistent southerly winds in the Bay of Bengal maintain the AR activity over Southwest China.The mid-to high-latitude AR response exhibits delayed dynamics,initially dominated by the synergistic effect of the southward deviation of the upper-air rapids and the low-level convergence(double-rapid-flow effect)and later modulated by the Pacific-North American teleconnection(PNA)-triggered East Asian ridge,which enhances the precipitation efficiency through prolonged frontal activity and enhanced cold-warm airmass convergence.Overall,El Niño promotes the development of low-and midlatitude AR activity in East Asia,while La Niña promotes(maritime continental)AR activity in the tropics.This study establishes the“ENSO teleconnection→circulation adjust-ment→East Asian AR response”chain,revealing a cross-seasonal lagged response mechanisms of East Asian AR activity,and provides a theoretical basis for winter and spring climate prediction and extreme precipitation forecasting. 展开更多
关键词 ENSO Atmospheric river Western Pacific subtropical high East Asia Lagged response
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Ningxia Museum
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《China Today》 2026年第2期76-79,共4页
At the Ningxia Museum,visitors can dive into the history of the mysterious Western Xia Dynasty,as well as cultural and ethnic exchanges that occured along the ancient Silk Road centuries ago.THE area of present-day Ni... At the Ningxia Museum,visitors can dive into the history of the mysterious Western Xia Dynasty,as well as cultural and ethnic exchanges that occured along the ancient Silk Road centuries ago.THE area of present-day Ningxia Hui Autonomous Region in northwest China was a vital corridor of the ancient Silk Road.Around a thousand years ago,Yuan Hao,leader of the Tangut people,established the Western Xia(also known as Xixia)Dynasty(1038-1227)on this land,which coexisted with the Song,Liao,and Jin dynasties across China for nearly 200 years. 展开更多
关键词 cultural ethnic exchanges Silk Road Ningxia Museum Yuan Hao Western Xia Dynasty Cultural Exchange
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Retraction: MicroRNA-148a Acts as a Tumor Suppressor in Osteosarcoma via Targeting Rho-Associated Coiled-Coil Kinase
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作者 Oncology Research Editorial Office 《Oncology Research》 2026年第1期622-622,共1页
The published article titled“MicroRNA-148a Acts as a Tumor Suppressor in Osteosarcoma via Targeting Rho-Associated Coiled-Coil Kinase”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1231–1243.DOI:10.3... The published article titled“MicroRNA-148a Acts as a Tumor Suppressor in Osteosarcoma via Targeting Rho-Associated Coiled-Coil Kinase”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1231–1243.DOI:10.3727/096504017X14850134190255 URL:https://www.techscience.com/or/v25n8/56908 Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases. 展开更多
关键词 cellular datawhere tumor suppressor western blots rho associated coiled coil kinase microrna cellular data OSTEOSARCOMA figures
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Corrigendum
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《Neural Regeneration Research》 2026年第3期922-922,共1页
Corrigendum:Epalrestat protects against diabetic peripheral neuropathy by alleviating oxidative stress and inhibiting polyol pathway https://doi.org/10.4103/NRR.NRR-D-25-00562 In the article titled“Epalrestat protect... Corrigendum:Epalrestat protects against diabetic peripheral neuropathy by alleviating oxidative stress and inhibiting polyol pathway https://doi.org/10.4103/NRR.NRR-D-25-00562 In the article titled“Epalrestat protects against diabetic peripheral neuropathy by alleviating oxidative stress and inhibiting polyol pathway,”published on pages 345-351 in Issue 2,Volume 11 of Neural Regeneration Research(Li et al.,2016),the Western blot bands in Figure 2A are incorrect. 展开更多
关键词 western blot bands diabetic peripheral neuropathy alleviating oxidative stress oxidative stress polyol pathway EPALRESTAT
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Neoproterozoic Overprinting on the Archean Western Dharwar Craton,Southern India:Records from LA-ICP-MS U-Pb Geochronology of Rutile and Monazite
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作者 HU Yuhua ZHAO Yan +5 位作者 LU Junsheng GOU Longlong PMGEORGE AO Wenhao ZHANG Chengli ZHAI Mingguo 《Acta Geologica Sinica(English Edition)》 2026年第1期82-98,共17页
This study provides new evidence for a significant Neoproterozoic tectono-thermal event in the southern Dharwar Craton,based on U-Pb dating of rutile and monazite from metamorphic rocks of the Sargur Group.Trace eleme... This study provides new evidence for a significant Neoproterozoic tectono-thermal event in the southern Dharwar Craton,based on U-Pb dating of rutile and monazite from metamorphic rocks of the Sargur Group.Trace element analysis of rutile in garnet-bearing amphibolite,amphibolite,garnet-bearing staurolite-kyanite schist,and grunerite-garnet schist suggests that their protoliths were primarily basaltic rocks,sediments with mafic component,pelitic sediments,and mixed sediments with both mafic and felsic components,respectively.Rutile U-Pb ages of 625±22 Ma(garnet-bearing amphibolite),752±5.1 Ma(amphibolite),836±4 Ma to 663±26 Ma(garnet-bearing staurolite kyanite schist)and 860±13 Ma(grunerite-garnet schist),along with a monazite U-Pb age of 789±4.4 Ma(garnet-bearing staurolite kyanite schist),record a significant Neoproterozoic(860-625 Ma)tectono-thermal event along the southern margin of the western Dharwar Craton.This event is most likely associated with aborted rift-related alkaline magmatism along the northern margin of the Southern Granulite Terrane(SGT)and the southern margin of the Dharwar Craton.Given the lack of direct evidence for the assembly of the Rodinia supercontinent in the Dharwar Craton and SGT,this Neoproterozoic event is unlikely to be related to the breakup of Rodinia. 展开更多
关键词 rutile and monazite U-Pb ages NEOPROTEROZOIC Sargur area western Dharwar Craton southern India
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Investigation of natural and anthropogenic effects on aerosols optical properties over the Western Pacific ocean by the research vessel KEXUE
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作者 Jinyuan Xin Yining Ma +6 位作者 Xiangguang Zhang Yongjing Ma Xiaoyan Wu Fangkun Wu Quan Liu Yilong Lyu Jiawei Jiang 《Journal of Environmental Sciences》 2026年第1期596-605,共10页
In winter 2018,an aerosol physicochemical experiment was conducted in the Western Pacific Ocean(WPO)aboard the Research Vessel KEXUE of Chinese Academy of Sciences.This study systematically investigated both natural a... In winter 2018,an aerosol physicochemical experiment was conducted in the Western Pacific Ocean(WPO)aboard the Research Vessel KEXUE of Chinese Academy of Sciences.This study systematically investigated both natural and anthropogenic effects on marine aerosols optical properties,as well as the applicability of multi-satellite products and IMPROVE equation.The averaged aerosol optical depth(AOD500 nm)was 0.31±0.16 andÅngström exponent440–675 nm was 0.29±0.30.In offshore China,significant anthropogenic emissions affected the marine environment.In remote WPO,dust aerosols transported from northern China,Siberia,Central Asia,and those settling from the upper troposphere originating from north Africa,Arabian peninsula,and western India,were dominant.The spatial trends of AOD were opposite in the mid-latitude and southern seas of WPO.The highest AOD,0.32±0.23,appeared along the coast of South Asia at mid-latitude,decreasing from offshore seas to remote oceans.In low-latitude and equatorial seas,AOD significantly increased from coast to remote oceans.Ångström exponent dropped significantly from the coast to remote oceans as anthropogenic influence diminished across the entire WPO.Correlation analysis showed that both MODIS-C6 and Himawari AOD prod-ucts showed similar applicability in coastal urban areas,while Himawari AOD is highly recommended for coastal background and marine environment due to its finer resolution.The extinction coefficient derived from PM_(2.5) chemical compositions using IMPROVE algorithm exhibited a significant correlation(R^(2)=0.58)with the con-currently measured AOD in the absence of long-distance transport,suggesting that the IMPROVE is a reasonable proxy of the columnar average of marine aerosol extinctions free from transport influences. 展开更多
关键词 Aerosol optical properties Natural and anthropogenic effects Improve algorithm Ship-borne experiment Western Pacific Ocean
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高精度生物检测技术的系统化整合:温控扩展、荧光灵敏度提升与定量标准化研究进展 被引量:1
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作者 杨坤鹏 赵晖 +4 位作者 王雷 王哲 蔡承伦 吕佩昀 王宝 《吉林医学》 2025年第6期1466-1469,共4页
生物检测技术是现代生物医学研究和临床诊断的基础,其精度和可靠性直接影响研究结果的科学性和诊断结果的准确性。随着精准医疗和个体化治疗的快速发展,对生物检测技术的精度要求日益提高。高精度生物检测技术的系统化整合不仅需要优化... 生物检测技术是现代生物医学研究和临床诊断的基础,其精度和可靠性直接影响研究结果的科学性和诊断结果的准确性。随着精准医疗和个体化治疗的快速发展,对生物检测技术的精度要求日益提高。高精度生物检测技术的系统化整合不仅需要优化各项技术的关键参数,还需要建立统一的标准化体系,以确保不同实验室、不同设备之间结果的可比性和一致性。 展开更多
关键词 PCR 流式细胞仪 WESTERN印迹 生物检测技术 系统化整合
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马疱疹病毒1型对BHK-21细胞自噬影响的研究
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作者 玄玉莹 庞涛 +5 位作者 付婧 丁玉林 王文龙 高娃 祁咏华 王金玲 《中国预防兽医学报》 北大核心 2025年第10期1073-1079,共7页
为研究马疱疹病毒1型(EHV-1)感染仓鼠肾纤维细胞(BHK-21)后对细胞自噬功能的影响,本实验首先通过不同感染量(MOI)的EHV-1感染BHK-21细胞48 h后,利用Western blot检测自噬蛋白LC3 II和LC3 I的表达情况,计算LC3 II/LC3 I值;以不同浓度的... 为研究马疱疹病毒1型(EHV-1)感染仓鼠肾纤维细胞(BHK-21)后对细胞自噬功能的影响,本实验首先通过不同感染量(MOI)的EHV-1感染BHK-21细胞48 h后,利用Western blot检测自噬蛋白LC3 II和LC3 I的表达情况,计算LC3 II/LC3 I值;以不同浓度的雷帕霉素(Rapa)和3-甲基腺嘌呤(3-MA)分别作用于BHK-21细胞,采用CCK-8法检测细胞活性,筛选最适药物浓度(Rapa 0.1μmol/L,3-MA 0.5μmol/L)后,经Western blot检测分析EHV-1感染BHK-21细胞与最适药物浓度作用后BHK-21细胞的LC3 II/LC3 I值。结果显示,不同感染剂量EHV-1感染BHK-21细胞48 h后,感染组LC3 II/LC3 I值极显著低于对照组(P<0.001);EHV-1感染组LC3 II/LC3 I值显著低于对照组(P<0.05),且极显著低于Rapa处理组(P<0.001),而与3-MA组无显著差异。利用EHV-1感染BHK-21细胞后,采用Western blot检测不同时间点自噬蛋白LC3和P62的表达情况,并计算LC3 II/LC3 I与P62/GAPDH值;将EHV-1感染过表达LC3B的BHK-21细胞,利用激光共聚焦显微镜观察感染后的聚集情况;以MOI 1 EHV-1感染BHK-21细胞后,利用透射电镜观察不同时间点的自噬体。Western blot结果显示,EHV-1感染BHK-21细胞12 h后LC3 II/LC3 I值均极显著高于对照组(P<0.05),24 h~60 h时LC3 II/LC3 I值均极显著低于对照组(P<0.01);12 h~36 h时P62/GAPDH值显著高于对照组(P<0.05);48 h、60 h的P62/GAPDH值与对照组无显著差异。激光共聚焦显微镜观察可见,感染EHV-112 h后的BHK-21细胞中GFP-LC3B聚集量多于24 h、36 h时的感染组和12 h时的对照组;24 h和36 h感染组的GFP-LC3B聚集少于同一时间的对照组。透射电镜观察结果显示,EHV-1感染BHK-21细胞12 h后,BHK-21细胞中的自噬体明显多于对照组;而感染36 h时BHK-21细胞中自噬体的数量少于对照组和感染12 h自噬体的数量。激光共聚焦和透射电镜观察结果与Western blot检测结果一致。上述结果首次证实,EHV-1感染BHK-21细胞12 h促进细胞自噬,24 h后抑制细胞自噬。本研究为深入探究细胞自噬对EHV-1复制和感染的调控机制奠定基础。 展开更多
关键词 马疱疹病毒1型 BHK-21细胞 细胞自噬 Western blot 透射电镜
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自体黄韧带干预TGF-β1/Smad3信号通路抑制兔硬膜外纤维瘢痕形成的实验研究
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作者 王鹏 张德宝 +5 位作者 张杨 张海斌 时嘉 张少杰 李树文 吴一民 《中国临床解剖学杂志》 北大核心 2025年第1期69-75,共7页
目的通过对不同组别的兔分别行椎板切除术,对比其各组术后3、6周TGF-β1、Smad3蛋白表达量以及两者相应的mRNA表达水平,从而探讨自体黄韧带抑制腰椎术后硬膜外纤维瘢痕形成与TGF-β1/Smad3信号通路的关系。方法将于内蒙古医科大学实验... 目的通过对不同组别的兔分别行椎板切除术,对比其各组术后3、6周TGF-β1、Smad3蛋白表达量以及两者相应的mRNA表达水平,从而探讨自体黄韧带抑制腰椎术后硬膜外纤维瘢痕形成与TGF-β1/Smad3信号通路的关系。方法将于内蒙古医科大学实验动物中心购买的48只6~8月龄的日本大白兔,分为保留黄韧带组,自体脂肪回置组,不保留黄韧带组,对不同组别进行手术造模。术后对每组间的实验动物分别饲养3、6周后进行处死(3、6周各处死8只)。采用Western blot蛋白定量分析、RTPCR技术测定各组样本中的TGF-β1、Smad3蛋白含量及mRNA表达水平,并将以上数据分别进行组间比较,分析其差异性。结果①Western blot检测结果:3、6周保留组TGF-β1、Smad3蛋白表达量小于不保留组和脂肪组(P<0.05),不保留组和脂肪组之间并未发现明显差异。②RT-PCR结果显示:对于TGF-β1、Smad3两者的mRNA表达量而言,3、6周保留组明显小于不保留组和脂肪组(P<0.05),不保留组和脂肪组之间并未见明显差异。结论腰椎手术术后,会形成不同程度的硬膜外纤维瘢痕。若在术中保留自体黄韧带,可减少硬膜外成纤维细胞的形成,其机制与TGF-β1/Smad3信号通路有关。 展开更多
关键词 黄韧带 纤维瘢痕 TGF-β1/Smad3信号通路 Western blot RT-PCR技术
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条件性胰岛β细胞HLF基因敲除小鼠构建与鉴定
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作者 侯梦龙 齐心语 +4 位作者 吴建凤 廖启超 马杰 周磊 李一星 《安徽医科大学学报》 北大核心 2025年第8期1432-1439,共8页
目的为了探究肝白血病因子(HLF)在糖尿病中的作用机制,构建条件性胰岛β细胞HLF基因敲除小鼠动物模型。方法在细胞水平上,通过CCK-8试验验证HLF抑制或过表达对MIN6细胞增殖的影响。通过RT-qPCR和Western blot,分别在mRNA水平和蛋白水平... 目的为了探究肝白血病因子(HLF)在糖尿病中的作用机制,构建条件性胰岛β细胞HLF基因敲除小鼠动物模型。方法在细胞水平上,通过CCK-8试验验证HLF抑制或过表达对MIN6细胞增殖的影响。通过RT-qPCR和Western blot,分别在mRNA水平和蛋白水平检测HLF抑制或过表达效果。将HLF flox/flox转基因小鼠与Pdx1-Cre+/-小鼠(C57BL/6J)杂交繁育,获得子代小鼠。利用PCR方法鉴定小鼠基因型,通过RT-qPCR技术和Western blot检测HLF基因在胰岛β细胞敲除小鼠(HLF flox/flox Cre+/-)和对照小鼠(HLF flox/flox)中的mRNA水平和蛋白水平表达差异,验证敲除效果。同时取2组小鼠的胰岛组织制作石蜡切片并进行苏木精-伊红(HE)染色分析。结果HLF基因抑制或过表达对MIN6细胞增殖无显著影响。在MIN6细胞中抑制HLF基因,其mRNA表达水平较对照组下降了74%,蛋白表达水平较对照组下降了60%;过表达HLF基因后,其mRNA表达水平为对照组的2.13倍,蛋白表达水平为对照组的1.8倍。敲除小鼠的HLF基因mRNA表达水平较对照组下降了89%,蛋白表达水平较对照组下降了65%。HE染色结果显示:敲除小鼠胰岛组织内细胞形态与对照小鼠无明显差异。抑制HLF使MIN6细胞中糖原含量提高约20%。结论成功构建HLF基因敲除小鼠,为研究HLF在糖尿病发病机制中的作用提供了动物模型。 展开更多
关键词 肝白血病因子 条件性胰岛β细胞 RT-QPCR Western blot 基因敲除 C57BL/6J小鼠
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CSF1R^(+/-)小鼠的构建和基因鉴定
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作者 周园园 刘崇 +4 位作者 王安琪 张慧茹 邱佳琪 朱梦娟 涂佳杰 《安徽医科大学学报》 北大核心 2025年第5期884-889,共6页
目的构建集落刺激因子1受体杂合(CSF1R^(+/-))小鼠并分析其基因型,为疾病病理机制及药物靶点提供动物模型基础。方法根据Cre/Loxp系统设计一种线性化的靶向载体,在集落刺激因子1受体(CSF1R)基因第5外显子上游插入一个Loxp位点,在第5外... 目的构建集落刺激因子1受体杂合(CSF1R^(+/-))小鼠并分析其基因型,为疾病病理机制及药物靶点提供动物模型基础。方法根据Cre/Loxp系统设计一种线性化的靶向载体,在集落刺激因子1受体(CSF1R)基因第5外显子上游插入一个Loxp位点,在第5外显子下游插入一个双侧有Loxp位点的新霉素抗性盒(PGK-neo)。将线性化的靶向载体电穿孔至胚胎干细胞(ES)。将正确靶向的ES注射到C57BL/6J小鼠的胚泡中得到嵌合小鼠,与透明带3-Cre(Zp3-Cre)小鼠进行繁殖。新生小鼠出生后9~14天编号并剪鼠尾,提取小鼠的DNA,通过聚合酶链式反应(PCR)和琼脂糖凝胶电泳鉴定小鼠基因型,筛选出CSF1R^(+/-)小鼠。应用流式细胞术检测小鼠巨噬细胞中CSF1R的表达;Western blot检测小鼠组织中CSF1R的蛋白表达。结果琼脂糖凝胶电泳结果显示,野生小鼠(WT)扩增出453 bp的条带,CSF1R^(+/-)小鼠扩增出453 bp和650 bp的条带。流式细胞术结果显示,与WT组比较,CSF1R^(+/-)组小鼠的腹腔来源巨噬细胞(PM)和骨髓来源巨噬细胞(BMDM)中的CSF1R低表达(P<0.05)。Western blot结果显示,与WT组比较,CSF1R^(+/-)组小鼠脾脏、肾脏、脑组织中的CSF1R蛋白低表达(P<0.05)。结论成功构建、繁育和鉴定CSF1R^(+/-)小鼠,为进一步揭示CSF1R在免疫调节中的潜在机制研究提供动物模型基础。 展开更多
关键词 CSF1R PCR Western blot 流式细胞术 Cre/Loxp 动物模型
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ODF1单克隆、多克隆抗体的制备及特异性检测
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作者 瞿成龙 向雨欣 +3 位作者 明胜利 曾磊 王江 褚贝贝 《山西农业科学》 2025年第6期159-165,共7页
为探究ODF1蛋白的生物学功能,制备并鉴定其特异性抗体工具,采用原核表达系统表达重组ODF1蛋白,将高纯度的重组ODF1蛋白作为免疫原,利用杂交瘤技术制备鼠源单克隆抗体,通过免疫新西兰兔制备多克隆抗体。采用Western blot和免疫荧光(IFA)... 为探究ODF1蛋白的生物学功能,制备并鉴定其特异性抗体工具,采用原核表达系统表达重组ODF1蛋白,将高纯度的重组ODF1蛋白作为免疫原,利用杂交瘤技术制备鼠源单克隆抗体,通过免疫新西兰兔制备多克隆抗体。采用Western blot和免疫荧光(IFA)对抗体性能进行系统评价。结果成功筛选出1A1-7、5C6-4等2株稳定分泌抗ODF1单克隆抗体的杂交瘤细胞株,亚型均为IgM,同时获得了高效价的兔源多克隆抗体。Western blot和免疫荧光分析结果表明,单克隆、多克隆抗体均能特异性识别内源性、外源性ODF1蛋白,表现出良好的特异性和应用潜力。综上,该试验成功制备了特异性良好的ODF1单克隆、多克隆抗体。 展开更多
关键词 原核表达 ODF1蛋白 单克隆抗体 Western blot 多克隆抗体 杂交瘤技术 免疫荧光
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Guidelines for the diagnosis and treatment of depressive disorders by integrating Chinese and Western medicine(English edition) 被引量:3
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作者 Lanying Liu Jianjun Wang +16 位作者 Wei Li Jing Gao Wangtao Li Yan Li Liyuan Luo Liyuan Guo Yiying Hu Yongjun Chen Hongyan Chen Lin Yu Bin Fen Hongxiao Jia Zhangjin Zhang Zhaojun Yan Wei Chen Zhangsheng Yu Zhen Wang 《General Psychiatry》 2025年第1期1-15,共15页
INTRODUCTION.Depressive disorders are mental illnesses that seriously affect public health.There are approximately 320 million patients with depression worldwide,accounting for 4.4% of the total disease burden.1Depres... INTRODUCTION.Depressive disorders are mental illnesses that seriously affect public health.There are approximately 320 million patients with depression worldwide,accounting for 4.4% of the total disease burden.1Depression leads to social and occupational impairment,diminished quality of life and an elevated risk of death by suicide. 展开更多
关键词 DIAGNOSIS mental illnesses depressive disorders chinese medicine western medicine TREATMENT
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短链脂肪酸异戊酸对痘苗病毒体外活性的影响
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作者 杜梦华 李云洁 +6 位作者 郭亚迪 梅航天 邵弓宇 尚雨欣 王江 褚贝贝 明胜利 《山西农业科学》 2025年第6期127-134,共8页
为探究短链脂肪酸异戊酸对痘苗病毒(VACV)体外增殖的抑制作用及其机制,采用直接免疫荧光法筛选5种短链脂肪酸的抗病毒效应;通过CCK-8法评估异戊酸(30~3000μmol/L)对BHK-21、Vero细胞活力的影响;PI染色结合流式细胞术分析异戊酸(10~100... 为探究短链脂肪酸异戊酸对痘苗病毒(VACV)体外增殖的抑制作用及其机制,采用直接免疫荧光法筛选5种短链脂肪酸的抗病毒效应;通过CCK-8法评估异戊酸(30~3000μmol/L)对BHK-21、Vero细胞活力的影响;PI染色结合流式细胞术分析异戊酸(10~100μmol/L)对细胞周期的干预作用。利用流式细胞术定量异戊酸处理细胞中VACV-GFP的增殖水平;采用Western blotting检测VACV结构蛋白A27L、GFP表达变化;采用实时荧光定量PCR分析病毒关键基因F17R、E9L的mRNA转录水平。结果表明,异戊酸显著抑制VACV增殖,且在30~3000μmol/L浓度下对细胞活力无显著影响,在10~100μmol/L浓度时未干扰细胞周期;West⁃ern blotting结果显示,异戊酸剂量依赖性抑制A27L、GFP蛋白表达;qPCR证实,其显著下调F17R、E9L的mRNA水平。综上,异戊酸通过抑制病毒蛋白合成及基因转录,有效阻断VACV体外增殖。 展开更多
关键词 短链脂肪酸 异戊酸 痘苗病毒 Western blotting 流式细胞术 抗病毒
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Gastrointestinal microbiota in treatment of gastric precancerous lesions and gastric cancer by Western and traditional Chinese medicine 被引量:1
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作者 Ting-Lan Cao Hui-Zhong Jiang 《World Journal of Clinical Oncology》 2025年第8期31-47,共17页
Gastric precancerous lesions(GPL)represent a crucial stage in the complex process of gastric carcinogenesis that leads to gastric cancer(GC),one of the most prevalent cancers and a major source of cancer mortality wor... Gastric precancerous lesions(GPL)represent a crucial stage in the complex process of gastric carcinogenesis that leads to gastric cancer(GC),one of the most prevalent cancers and a major source of cancer mortality worldwide.Many studies have identified the gastrointestinal microbiota,or gut microbiota,as an important contributor to both the pathogenesis and treatment of GPL and GC,thus understanding its role in this transition is crucial.The purpose of this literature review is to introduce the current landscape of microbiota research associated with GPL and GC,with an emphasis on Helicobacter pylori(H.pylori)driven microbial dysbiosis and its modulation through Western medicine and traditional Chinese medicine(TCM)approaches.By elucidating the underlying mechanisms of H.pylori colonization,patterns,and interactions among microbiota,as well as the influence of microbial metabolites,this review highlights crucial driving factors of gastric carcinogenesis.The role of microbiota in conventional interventions,including H.pylori eradication,immunotherapy,as well as TCM herbal decoctions,is also discussed to provide a detailed understanding of the complex interactions between therapy and microbiota and how it could be potentially targeted for effective management of GPL and GC.Ultimately,microbiota-targeting therapeutics may represent a new path toward early detection,targeted treatment,improved prognosis,and potentially reduced incidence of GPL and GC. 展开更多
关键词 Gastric precancerous lesions Gastric cancer MICROBIOTA DYSBIOSIS Western medicine Traditional Chinese medicine
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Deep Velocity Structure and Tectonic Characteristics of the Pamir Plateau based on Bayesian Inversion 被引量:1
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作者 HAILAI Muguo LIANG Feng +5 位作者 HAN Chen Davlatkhudzha MURODOV FANG Lihua Sherzod ABDULOV YAN Jiayong AN Yanru 《Acta Geologica Sinica(English Edition)》 2025年第6期1556-1574,共19页
The Pamir Plateau,at the northwestern margin of the Tibetan Plateau,is a key region for investigating continental collision and plateau uplifting.To probe its deep structure,we collected seismic data from 263 stations... The Pamir Plateau,at the northwestern margin of the Tibetan Plateau,is a key region for investigating continental collision and plateau uplifting.To probe its deep structure,we collected seismic data from 263 stations across 11 research projects.We applied cross-correlation to noise data and extracted surface wave dispersion data from cross-correlation functions.The extracted dispersion data were subsequently inverted using a 3-D transdimensional Bayesian inversion method(rj-3 DMcMC).The inversion result reveals several crustal low-velocity zones(LVZs).Their formation is likely related to crustal thickening,the exposure of gneiss domes,and thicker sedimentary sequences compared to surrounding areas.In the lower crust and upper mantle,the LVZs in southern Pamir and southeastern Karakoram evolve into high-velocity zones,which expand northeastward with increasing depth.This suggests northward underthrusting of the Indian Plate.We also analyzed the Moho using both the standard deviation of S-wave velocity and the S-wave velocity structure.Results show that significant variations in velocity standard deviation reliably delineate the Moho interface. 展开更多
关键词 ambient noise tomography Bayesian inversion crust and mantle structure Western Himalayan syntaxis
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猪衣原体ompA基因的原核表达与纯化及其蛋白功能预测
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作者 李宁倩 禹思宇 +6 位作者 李桐菲 李文超 魏颖 谭建锡 胡忆文 唐连飞 刘伟 《湖南畜牧兽医》 2025年第5期29-33,共5页
为了将猪衣原体ompA基因作为目标抗原,为后续建立间接ELISA检测方法提供理论依据,开展了该基因的原核表达与纯化工作,并利用生物信息学方法对ompA蛋白的理化性质、亲疏水性、跨膜区域和抗原表位等进行了分析与预测。结果显示:该研究成... 为了将猪衣原体ompA基因作为目标抗原,为后续建立间接ELISA检测方法提供理论依据,开展了该基因的原核表达与纯化工作,并利用生物信息学方法对ompA蛋白的理化性质、亲疏水性、跨膜区域和抗原表位等进行了分析与预测。结果显示:该研究成功构建了重组质粒,并通过SDS-PAGE和Western blot确认该蛋白以包涵体形式表达,分子量约为27 kDa;该蛋白的预测分子质量为23.31921 kDa,理论等电点为4.37,不含跨膜区,属于亲水性蛋白,含有7个抗原决定簇,平均抗原倾向值为1.0316。结果表明:ompA蛋白具备良好的免疫反应性,是免疫反应中的关键成分,适合作为间接ELISA的包被抗原。该研究为深入理解猪衣原体的致病机制及开发相应的防控技术提供了理论基础。 展开更多
关键词 猪衣原体ompA基因 重组质粒 表达及纯化 Western blot验证 生物信息学预测 间接ELISA
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The strongest early-summer drought–flood abrupt alternation event over the Huang–Huai–Hai River Basin in 2024 since the 1980s:Perspective of anomalous subseasonal circulation evolution 被引量:1
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作者 Zhiheng Chen Zhihai Zheng 《Atmospheric and Oceanic Science Letters》 2025年第5期65-72,共8页
Since the 21st century,the Huang–Huai–Hai River Basin(HHHRB)in China has experienced increased frequency and severity of drought–flood abrupt alternation(DFAA)events during early summer,characterized by droughts in... Since the 21st century,the Huang–Huai–Hai River Basin(HHHRB)in China has experienced increased frequency and severity of drought–flood abrupt alternation(DFAA)events during early summer,characterized by droughts in June followed by floods in July.The 2024 event was the most severe since 1981.This study demonstrates that such compound extreme events are closely linked to anomalous subseasonal evolution of large-scale atmospheric circulation.During the drought phase,the East Asian subtropical westerly jet(EAJ)shifts southward,and the western Pacific subtropical high(WPSH)exhibits anomalous strengthening with its western ridge line displaced southward.The flood phase is characterized by acceleration of the EAJ,westward extension of the WPSH,and enhanced southwestern moisture transport from the western Pacific.Beyond these typical features,the 2024 early summer circulation exhibited unique characteristics:Anomalous northeastward intensification of the WPSH facilitated merged moisture influx from both the Indian Ocean and the western Pacific along the southeast pathway into the HHHRB in July,resulting in the highest net moisture inflow at the southern boundary of the HHHRB since 1981.The synergistic effects of multiple factors primarily explain the exceptionally intense DFAA event in 2024. 展开更多
关键词 Drought-flood abrupt alternation Anomalous subseasonal circulation evolution East Asian subtropical westerly jet Western Pacific subtropical high Water vapor transportation
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基于网络药理学和实验验证探究杏贝止咳祛痰口服液治疗儿童支气管炎的作用机制
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作者 杨子烨 谭征 +4 位作者 刘洁 刘润花 杨畅 关晶 张建民 《中南药学》 2025年第8期2165-2172,共8页
目的运用网络药理学、分子对接以及体外细胞实验探究杏贝止咳祛痰口服液治疗儿童支气管炎的作用机制。方法检索TCMSP、BATMAN-TCM、PubChem、SwissTargetPrediction等数据库,获得杏贝止咳祛痰口服液活性成分、相关作用靶点以及儿童支气... 目的运用网络药理学、分子对接以及体外细胞实验探究杏贝止咳祛痰口服液治疗儿童支气管炎的作用机制。方法检索TCMSP、BATMAN-TCM、PubChem、SwissTargetPrediction等数据库,获得杏贝止咳祛痰口服液活性成分、相关作用靶点以及儿童支气管炎相应疾病靶点,并将杏贝止咳祛痰口服液作用靶点与疾病靶点的交集作为潜在作用靶点;利用Cytoscape软件构建成分-疾病网络及蛋白互作网络,分析其网络拓扑结构,筛选关键成分及核心靶点,并进行分子对接验证;采用R语言对交集靶点进行GO功能及KEGG富集分析。最后,基于香烟烟雾提取物(CSE)诱导人正常支气管上皮细胞16-HBE构建体外细胞模型,采用Western blot技术进行相关通路靶点的验证。结果杏贝止咳祛痰口服液关键成分包括光甘草定、槲皮素、山柰酚等,主要通过调控TNF-α、STAT3、EGFR、PTGS2等在内的核心靶点介导的AGE-RAGE等信号通路发挥治疗儿童支气管炎的作用。Western blot实验结果显示,与空白组相比,模型组TNF-α、STAT3、MAPK3、ICAM1和PIK3CD蛋白的表达显著上调(P<0.01);与模型组相比,杏贝止咳祛痰口服液给药组对应蛋白显著下调(P<0.01)。结论杏贝止咳祛痰口服液可通过多成分、多靶点、多途径改善儿童支气管炎临床症状,其作用机制可能与抑制AGE-RAGE信号传导通路的活化有关。 展开更多
关键词 儿童支气管炎 杏贝止咳祛痰口服液 网络药理学 Western blot AGE-RAGE信号通路
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