Background:Postmortem diagnosis of anaphylactic shock remains one of the challenges in forensic pathology due to the absence of specific pathological and morphological changes.MicroRNAs,characterized by tissue-specifi...Background:Postmortem diagnosis of anaphylactic shock remains one of the challenges in forensic pathology due to the absence of specific pathological and morphological changes.MicroRNAs,characterized by tissue-specific expression and resistance to degradation,have been applied in forensic medicine for postmortem interval estimation and auxiliary diagnosis of certain causes of death.However,their utility in assisting the diagnosis of anaphylactic shock as a cause of death has not been previously reported in the literature.Aims and Objectives:This study aimed to identify relevant microRNAs by constructing a search strategy for retrieval in the PubMed database and to validate and screen the selected indicators using collected blood samples.Materials and Methods:A literature search strategy was developed with a focus on"anaphylactic shock"and"microRNA"in PubMed.Relevant literatures were screened,and microRNAs were extracted and compiled.Blood samples were collected from 8 cases of death due to anaphylactic shock and 22 cases of death from nonanaphylactic causes.RNA was extracted,and RT-PCR was performed to detect microRNAs.Relative expression levels were calculated for statistical analysis.Results:A total of 15 relevant literatures were retrieved from PubMed.After extracting microRNAs and referencing with the miRBase and miRDB databases,22 microRNAs were finally identified.Validation using postmortem blood samples revealed that the expression levels of 11 microRNAs were significantly higher in the anaphylactic shock group than in the control group(P<0.05).ROC analysis showed that 9 of 11 microRNAs had diagnostic efficacy,including miR-149-5p,miR-34a-5p,miR-181a-5p,miR-487b-3p,miR-182-5p,miR-154-5p,miR-451a,miR-155-3p,and miR-155-5p(P<0.05).Among them,miR-182-5p exhibited the optimal diagnostic efficacy(AUC=0.97).Conclusion:Postmortem detection of 9 microRNAs(miR-149-5p,miR-34a-5p,miR-181a-5p,miR-487b-3p,miR-182-5p,miR-154-5p,miR-451a,miR-155-3p,and miR-155-5p)in blood samples holds potential for assisting in the postmortem diagnosis of anaphylactic shock.展开更多
Cloud computing has become a significant computing model in the IT industry. In this emerging model,computing resources such as software, hardware, networking, and storage can be accessed anywhere in the world on a pa...Cloud computing has become a significant computing model in the IT industry. In this emerging model,computing resources such as software, hardware, networking, and storage can be accessed anywhere in the world on a pay-per-use basis. However, storing sensitive data on un-trusted servers is a challenging issue for this model. To guarantee confidentiality and proper access control of outsourced sensitive data, classical encryption techniques are used. However, such access control schemes are not feasible in cloud computing because of their lack of flexibility, scalability, and fine-grained access control. Instead, Attribute-Based Encryption（ABE） techniques are used in the cloud. This paper extensively surveys all ABE schemes and creates a comparison table for the key criteria for these schemes in cloud applications.展开更多
To improve the performance of extended particle swarm optimizer, a novel means of stochastic weight deployment is proposed for the iterative equation of velocity updation. In this scheme, one of the weights is specifi...To improve the performance of extended particle swarm optimizer, a novel means of stochastic weight deployment is proposed for the iterative equation of velocity updation. In this scheme, one of the weights is specified to a random number within the range of [0, 1] and the other two remain constant configurations. The simulations show that this weight strategy outperforms the previous deterministic approach with respect to success rate and convergence speed. The experiments also reveal that if the weight for global best neighbor is specified to a stochastic number, extended particle swarm optimizer achieves high and robust performance on the given multi-modal function.展开更多
文摘Background:Postmortem diagnosis of anaphylactic shock remains one of the challenges in forensic pathology due to the absence of specific pathological and morphological changes.MicroRNAs,characterized by tissue-specific expression and resistance to degradation,have been applied in forensic medicine for postmortem interval estimation and auxiliary diagnosis of certain causes of death.However,their utility in assisting the diagnosis of anaphylactic shock as a cause of death has not been previously reported in the literature.Aims and Objectives:This study aimed to identify relevant microRNAs by constructing a search strategy for retrieval in the PubMed database and to validate and screen the selected indicators using collected blood samples.Materials and Methods:A literature search strategy was developed with a focus on"anaphylactic shock"and"microRNA"in PubMed.Relevant literatures were screened,and microRNAs were extracted and compiled.Blood samples were collected from 8 cases of death due to anaphylactic shock and 22 cases of death from nonanaphylactic causes.RNA was extracted,and RT-PCR was performed to detect microRNAs.Relative expression levels were calculated for statistical analysis.Results:A total of 15 relevant literatures were retrieved from PubMed.After extracting microRNAs and referencing with the miRBase and miRDB databases,22 microRNAs were finally identified.Validation using postmortem blood samples revealed that the expression levels of 11 microRNAs were significantly higher in the anaphylactic shock group than in the control group(P<0.05).ROC analysis showed that 9 of 11 microRNAs had diagnostic efficacy,including miR-149-5p,miR-34a-5p,miR-181a-5p,miR-487b-3p,miR-182-5p,miR-154-5p,miR-451a,miR-155-3p,and miR-155-5p(P<0.05).Among them,miR-182-5p exhibited the optimal diagnostic efficacy(AUC=0.97).Conclusion:Postmortem detection of 9 microRNAs(miR-149-5p,miR-34a-5p,miR-181a-5p,miR-487b-3p,miR-182-5p,miR-154-5p,miR-451a,miR-155-3p,and miR-155-5p)in blood samples holds potential for assisting in the postmortem diagnosis of anaphylactic shock.
文摘Cloud computing has become a significant computing model in the IT industry. In this emerging model,computing resources such as software, hardware, networking, and storage can be accessed anywhere in the world on a pay-per-use basis. However, storing sensitive data on un-trusted servers is a challenging issue for this model. To guarantee confidentiality and proper access control of outsourced sensitive data, classical encryption techniques are used. However, such access control schemes are not feasible in cloud computing because of their lack of flexibility, scalability, and fine-grained access control. Instead, Attribute-Based Encryption（ABE） techniques are used in the cloud. This paper extensively surveys all ABE schemes and creates a comparison table for the key criteria for these schemes in cloud applications.
基金the Natural Science Foundation of the Anhui Higher Education Institutions (KJ2008B151)Key Laboratory of Information Management and Information Economics, Ministry of Education (F0607-36)
文摘To improve the performance of extended particle swarm optimizer, a novel means of stochastic weight deployment is proposed for the iterative equation of velocity updation. In this scheme, one of the weights is specified to a random number within the range of [0, 1] and the other two remain constant configurations. The simulations show that this weight strategy outperforms the previous deterministic approach with respect to success rate and convergence speed. The experiments also reveal that if the weight for global best neighbor is specified to a stochastic number, extended particle swarm optimizer achieves high and robust performance on the given multi-modal function.
