In order to illustrate the ion transport mechanism of chloride channel（Cl C） protein,a type of Cl C protein,Cl C-ec1,from Escherichia coli is embedded into an explicit membranewater system by using software VMD. The...In order to illustrate the ion transport mechanism of chloride channel（Cl C） protein,a type of Cl C protein,Cl C-ec1,from Escherichia coli is embedded into an explicit membranewater system by using software VMD. Then a parallel molecular dynamics（MD） simulation is employed to equilibrate the Cl C-ec1 structure for 27.5 ns at temperature 298.15 K. Based on this equilibrated structure,we compute the channel geometric size variation and electrostatic potential distribution along the channel. Meanwhile,Cl^- transport process is simulated using oriented random walk method under variable external potential. The simulation result shows that Cl^- transport velocity depends on the width of the narrowest channel region. Mutation of negative glutamate E148 can produce positive potential,which is beneficial for Cl^- transport,around external Cl^- binding region in the channel. The simulated current-voltage curves about Cl^- transporting in Cl C-ec1 protein agree with Jayaram＇s experimental results.展开更多
In this essay I focus on a subject that has not been treated at any great length in the past,that is,the relationship between magical realism and the videogame.This is surprising because even a cursory glance at the t...In this essay I focus on a subject that has not been treated at any great length in the past,that is,the relationship between magical realism and the videogame.This is surprising because even a cursory glance at the types of videogames that are currently available on the market shows that they are full of techniques and surprises and wonders that are characteristic of the genre of magical realism.This essay focuses on the overlap between the videogame genre and other related genres such as literature and film,particularly in terms of its use of the technique of magic.展开更多
基金Supported by the National Natural Science Foundation of China(11304123)the Scientific Research Foundation of Jianghan University(2013016)
文摘In order to illustrate the ion transport mechanism of chloride channel（Cl C） protein,a type of Cl C protein,Cl C-ec1,from Escherichia coli is embedded into an explicit membranewater system by using software VMD. Then a parallel molecular dynamics（MD） simulation is employed to equilibrate the Cl C-ec1 structure for 27.5 ns at temperature 298.15 K. Based on this equilibrated structure,we compute the channel geometric size variation and electrostatic potential distribution along the channel. Meanwhile,Cl^- transport process is simulated using oriented random walk method under variable external potential. The simulation result shows that Cl^- transport velocity depends on the width of the narrowest channel region. Mutation of negative glutamate E148 can produce positive potential,which is beneficial for Cl^- transport,around external Cl^- binding region in the channel. The simulated current-voltage curves about Cl^- transporting in Cl C-ec1 protein agree with Jayaram＇s experimental results.
文摘In this essay I focus on a subject that has not been treated at any great length in the past,that is,the relationship between magical realism and the videogame.This is surprising because even a cursory glance at the types of videogames that are currently available on the market shows that they are full of techniques and surprises and wonders that are characteristic of the genre of magical realism.This essay focuses on the overlap between the videogame genre and other related genres such as literature and film,particularly in terms of its use of the technique of magic.
