In vitro experiment using excised skin has been valuable for studying the mechanism of percutaneous absorption. Based on previously established static diffusion cell system in this laboratory, a novel model-peifused g...In vitro experiment using excised skin has been valuable for studying the mechanism of percutaneous absorption. Based on previously established static diffusion cell system in this laboratory, a novel model-peifused glass diffusion cell system is desboed. The results of initial comporative study on percutaneous absorption between glass perfused diffusion cell and static diffusion cell, ih vitro and in vivo permeation as well as factors affecting permeation with seven radiolabelled chemicals are presented. The results demonstrate that the peifused diffusion cell system, which used a perfusion nuid betow the suiface of skin to take up the materials which penetrated the skin, is more similar to physiologic condition,convenient and automatic than that of the static cell. It well predicts the in vivo percutaneous absorption if appropriate areptor fluid is chosen. The results also show that the selection of receptor fluid is critical for in vitro permeation of chemicals with different soubility展开更多
In this paper, instrumental analysis techniques for hair fibers and biological models for scalp were introduced as in vitro techniques applied for the efficacy evaluation of hair cosmetics. This review aimed to provid...In this paper, instrumental analysis techniques for hair fibers and biological models for scalp were introduced as in vitro techniques applied for the efficacy evaluation of hair cosmetics. This review aimed to provide reference and guidance for the selection of appropriate in vitro methods to substantiate the performance of various hair cosmetics. The current status and development trends of in vitro evaluation technology for hair cosmetics were summarized and prospected.展开更多
Para-phenylenediamine （PPD）, tile main toxic ingredients of hair dyes, have been used by millions of consumers to improve their appearance. Stone Hair Dye （SHD） mainly contain PPD. SHD and PPD were evaluated by 3-...Para-phenylenediamine （PPD）, tile main toxic ingredients of hair dyes, have been used by millions of consumers to improve their appearance. Stone Hair Dye （SHD） mainly contain PPD. SHD and PPD were evaluated by 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyltetrazdium bromide or MTT assay, which measures mitochondria metabolism in the entire cell culture and provides information about the percentage of cell survival. Utilizing the MTT assay, the cytotoxicity of SHD and PPD was determining on SH Sy5y culture from nervous system of rat. The short term exposure SH Sy5y culture were incubated with various aqueous solutions of different concentrations of SHD and PPD, and the LC50 of SHD and para-phenylenediamine was found to be 9.15 and 12.4 mg/ml. With increasing the concentration, cytotoxicity effect increase in PPD and SHD. There is a significant differences （P〈0.01） of cytotoxicity among concentrations used. It concluded that in vitro assay could give important information of the mechanism of toxicity at low dosages.展开更多
To evaluate and discuss two novels in vitro alternative tests which based on the 2nd and 4th event of the AOP in skin sensitization and their application in skin sensitization evaluation of cosmetics in vitro.The DSen...To evaluate and discuss two novels in vitro alternative tests which based on the 2nd and 4th event of the AOP in skin sensitization and their application in skin sensitization evaluation of cosmetics in vitro.The DSens(DSens method)and Jurkat(TCPA method)were used as the test models and 9 of reference chemicals and 12 kinds of cosmetic products were used to confirm and assess the application capability in skin sensitization.The results showed that the DSens method was more sensitive to the reference chemicals compare to the TCPA method.All the results of cosmetic products showed a high consistency between these two assays and h-CLAT or in vivo assay.As the new screening method for skin sensitization evaluation of cosmetics,the in vitro alternative tests based on AOP have certain effectiveness.The reasonable combination strategy can bring a bright future for the development and application of animal alternative test in China.展开更多
长效注射剂在治疗慢性疾病方面具有优势,本文对体外评价研究、体内评价研究的相关方法和影响因素进行了梳理,总结了体外释放行为与体内药动学过程的联立和数学建模,综述长效注射剂体内外相关性(in vitro-in vivo correlation,IVIVC)的...长效注射剂在治疗慢性疾病方面具有优势,本文对体外评价研究、体内评价研究的相关方法和影响因素进行了梳理,总结了体外释放行为与体内药动学过程的联立和数学建模,综述长效注射剂体内外相关性(in vitro-in vivo correlation,IVIVC)的分类、实验方法、建立步骤和模型评价方法。由于长效注射剂的多样性,IVIVC研究中不存在通用的体外模型,因此IVIVC研究只能进行逐例建模。综合药物理化性质、剂型设计和药物制剂与给药部位的相互作用对建立长效注射剂IVIVC研究十分重要。展开更多
文摘In vitro experiment using excised skin has been valuable for studying the mechanism of percutaneous absorption. Based on previously established static diffusion cell system in this laboratory, a novel model-peifused glass diffusion cell system is desboed. The results of initial comporative study on percutaneous absorption between glass perfused diffusion cell and static diffusion cell, ih vitro and in vivo permeation as well as factors affecting permeation with seven radiolabelled chemicals are presented. The results demonstrate that the peifused diffusion cell system, which used a perfusion nuid betow the suiface of skin to take up the materials which penetrated the skin, is more similar to physiologic condition,convenient and automatic than that of the static cell. It well predicts the in vivo percutaneous absorption if appropriate areptor fluid is chosen. The results also show that the selection of receptor fluid is critical for in vitro permeation of chemicals with different soubility
基金supported by the National Natural Science Foundation of China (51403006)the Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th Five–year Plan (CIT&TCD 201704043)
文摘In this paper, instrumental analysis techniques for hair fibers and biological models for scalp were introduced as in vitro techniques applied for the efficacy evaluation of hair cosmetics. This review aimed to provide reference and guidance for the selection of appropriate in vitro methods to substantiate the performance of various hair cosmetics. The current status and development trends of in vitro evaluation technology for hair cosmetics were summarized and prospected.
文摘Para-phenylenediamine （PPD）, tile main toxic ingredients of hair dyes, have been used by millions of consumers to improve their appearance. Stone Hair Dye （SHD） mainly contain PPD. SHD and PPD were evaluated by 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyltetrazdium bromide or MTT assay, which measures mitochondria metabolism in the entire cell culture and provides information about the percentage of cell survival. Utilizing the MTT assay, the cytotoxicity of SHD and PPD was determining on SH Sy5y culture from nervous system of rat. The short term exposure SH Sy5y culture were incubated with various aqueous solutions of different concentrations of SHD and PPD, and the LC50 of SHD and para-phenylenediamine was found to be 9.15 and 12.4 mg/ml. With increasing the concentration, cytotoxicity effect increase in PPD and SHD. There is a significant differences （P〈0.01） of cytotoxicity among concentrations used. It concluded that in vitro assay could give important information of the mechanism of toxicity at low dosages.
文摘To evaluate and discuss two novels in vitro alternative tests which based on the 2nd and 4th event of the AOP in skin sensitization and their application in skin sensitization evaluation of cosmetics in vitro.The DSens(DSens method)and Jurkat(TCPA method)were used as the test models and 9 of reference chemicals and 12 kinds of cosmetic products were used to confirm and assess the application capability in skin sensitization.The results showed that the DSens method was more sensitive to the reference chemicals compare to the TCPA method.All the results of cosmetic products showed a high consistency between these two assays and h-CLAT or in vivo assay.As the new screening method for skin sensitization evaluation of cosmetics,the in vitro alternative tests based on AOP have certain effectiveness.The reasonable combination strategy can bring a bright future for the development and application of animal alternative test in China.
文摘长效注射剂在治疗慢性疾病方面具有优势,本文对体外评价研究、体内评价研究的相关方法和影响因素进行了梳理,总结了体外释放行为与体内药动学过程的联立和数学建模,综述长效注射剂体内外相关性(in vitro-in vivo correlation,IVIVC)的分类、实验方法、建立步骤和模型评价方法。由于长效注射剂的多样性,IVIVC研究中不存在通用的体外模型,因此IVIVC研究只能进行逐例建模。综合药物理化性质、剂型设计和药物制剂与给药部位的相互作用对建立长效注射剂IVIVC研究十分重要。
