Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To estimate the possibility of cryopreservation for larvae of C. s...Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To estimate the possibility of cryopreservation for larvae of C. sauliae, which is a potential preparation for its artificial reproduction and further research, in this study a protocoi for the cryopreservation of veliger larvae of trumpet shell was optimized. Through a two-step cryopreservation procedure, four kinds of cryoprotectants （ethylene glycol, 1, 2-propanediol, dimethyl sulfoxide and glycerol） were employed at three concentrations （1.0, 1.5 and 2.0molL^-1） respectively and survival rates of larvae were determined after a storage of lh. The larvae frozen with these four cryoprotectants after 1 h storage were cultured, and then survival rates were determined at 24, 72 and 120 h after thawing. Dimethyl sulfoxide at a concentration of 1.5 molL^-1 showed the best protective effect in all experiments （p〈0.05）. And survival rates of larvae frozen with dimethyl sulfoxide were determined after 1, 7 and 15 d of storage. The survival rates of larvae frozen with 1.5 molL^-1 dimethyl sulfoxide after 1 h, 1 d, 7 d and 15 d of storage were 80.77% ±7.51%, 80.34%±11.28%, 83.10%±9.14% and 77.23%±6.22% respectively. No significant differences in survival rates of larvae frozen with dimethyl sulfoxide were observed after various storage periods （p〉0.05）.展开更多
Objective:To find the possible way to predict the mode of embryonic development of nudibranchs,focusing on egg mass characteristics,embryonic development,and shell patterns of the veligers.Methods:Eight species of nud...Objective:To find the possible way to predict the mode of embryonic development of nudibranchs,focusing on egg mass characteristics,embryonic development,and shell patterns of the veligers.Methods:Eight species of nudibranch were collected in the eastern part of the Gulf of Thailand.The specimens were allowed to copulate and lay their egg masses under laboratory conditions.Embryonic development was monitored under a light microscope with a digital camera every day until hatching.Results:Most of the species of nudibranch collected had a single larva in each egg capsule that developed,except for Jorunna funebris,which had 1-4 larvae in each egg capsule.All the specimens had the same pattern of cell division and hatching into the water column during the veliger form.However,the species developed at different rates in each stage.Conclusions:All species in the current study had planktotrophic development except Doriprismatica atromarginata,which showed lecithotrophic development.Based on embryonic development among the nudibranchs that showed planktotrophic development,Jorunna funebris appeared to be the most advantageous species for culture development with regard to utilization and conservation in the future.展开更多
文摘Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To estimate the possibility of cryopreservation for larvae of C. sauliae, which is a potential preparation for its artificial reproduction and further research, in this study a protocoi for the cryopreservation of veliger larvae of trumpet shell was optimized. Through a two-step cryopreservation procedure, four kinds of cryoprotectants （ethylene glycol, 1, 2-propanediol, dimethyl sulfoxide and glycerol） were employed at three concentrations （1.0, 1.5 and 2.0molL^-1） respectively and survival rates of larvae were determined after a storage of lh. The larvae frozen with these four cryoprotectants after 1 h storage were cultured, and then survival rates were determined at 24, 72 and 120 h after thawing. Dimethyl sulfoxide at a concentration of 1.5 molL^-1 showed the best protective effect in all experiments （p〈0.05）. And survival rates of larvae frozen with dimethyl sulfoxide were determined after 1, 7 and 15 d of storage. The survival rates of larvae frozen with 1.5 molL^-1 dimethyl sulfoxide after 1 h, 1 d, 7 d and 15 d of storage were 80.77% ±7.51%, 80.34%±11.28%, 83.10%±9.14% and 77.23%±6.22% respectively. No significant differences in survival rates of larvae frozen with dimethyl sulfoxide were observed after various storage periods （p〉0.05）.
基金funded by the Center of Advanced Studies in Tropical Natural Resources,KU Institute for Advanced Studies,Kasetsart University and Faculty of Technology,Mahasarakham University.
文摘Objective:To find the possible way to predict the mode of embryonic development of nudibranchs,focusing on egg mass characteristics,embryonic development,and shell patterns of the veligers.Methods:Eight species of nudibranch were collected in the eastern part of the Gulf of Thailand.The specimens were allowed to copulate and lay their egg masses under laboratory conditions.Embryonic development was monitored under a light microscope with a digital camera every day until hatching.Results:Most of the species of nudibranch collected had a single larva in each egg capsule that developed,except for Jorunna funebris,which had 1-4 larvae in each egg capsule.All the specimens had the same pattern of cell division and hatching into the water column during the veliger form.However,the species developed at different rates in each stage.Conclusions:All species in the current study had planktotrophic development except Doriprismatica atromarginata,which showed lecithotrophic development.Based on embryonic development among the nudibranchs that showed planktotrophic development,Jorunna funebris appeared to be the most advantageous species for culture development with regard to utilization and conservation in the future.
