The plasmid pGSA1285 was first modified by substituting its GUS sequence with the Chalcone synthase intron fragment from vector pFGC5941 to get the plant silencing expression vector that contained Kanamycin resistance...The plasmid pGSA1285 was first modified by substituting its GUS sequence with the Chalcone synthase intron fragment from vector pFGC5941 to get the plant silencing expression vector that contained Kanamycin resistance site and was named as pGSA2285. Using PCR-based amplification, two different restriction sites at both ends of tobacco Bax inhibitor-1 （NtBI-I） gene were created, respectively, which made the construction of ihpRNA gene silencing vector more efficiently. Then, NtBI-1 genes were inserted into Multiple Cloning Site （MCS） of pGSA2285 respectively to form Bax inhibitor-1 ihpRNA gene silencing vector, named as pGSA4285, containing sense and anti-sense BI-1 sequence which was spliced by chalcone synthase intron. Combined PCR identification and enzyme restriction analyses, the results showed that Bax inhibitor-1 ihpRNA gene silencing vector had been constructed and transferred into Agrobacterium tumefaciens EHA105 successfully, which laid a foundation for the further study on the function of BI-1 in plant PCD regulation.展开更多
目的克隆人胰腺癌hedgehog信号通路中PTCH基因,构建PTCH基因表达载体并诱导融合蛋白表达。方法从人胰腺癌细胞株SW1990抽提总RNA,经RT-PCR扩增出PTCH基因,经纯化、回收目的基因PTCH,将其插入表达载体PET22b,转化E.coliBL21-CodonPlusTM-...目的克隆人胰腺癌hedgehog信号通路中PTCH基因,构建PTCH基因表达载体并诱导融合蛋白表达。方法从人胰腺癌细胞株SW1990抽提总RNA,经RT-PCR扩增出PTCH基因,经纯化、回收目的基因PTCH,将其插入表达载体PET22b,转化E.coliBL21-CodonPlusTM-RP,构建重组质粒PET22b/PTCH,IPTG诱导表达融合蛋白,免疫印迹进行鉴定。结果从人胰腺癌细胞株SW1990克隆出长为789 bp PTCH目的片段,成功构建重组质粒PET22b/PTCH,并诱导表达目的蛋白。结论构建重组质粒PET22b/PTCH,并表达PTCH融合蛋白,为制备PTCH多克隆抗体打下良好基础。展开更多
基金Supported by National Natural Science Foundation of China (30500352)China Agricultural University URP Project
文摘The plasmid pGSA1285 was first modified by substituting its GUS sequence with the Chalcone synthase intron fragment from vector pFGC5941 to get the plant silencing expression vector that contained Kanamycin resistance site and was named as pGSA2285. Using PCR-based amplification, two different restriction sites at both ends of tobacco Bax inhibitor-1 （NtBI-I） gene were created, respectively, which made the construction of ihpRNA gene silencing vector more efficiently. Then, NtBI-1 genes were inserted into Multiple Cloning Site （MCS） of pGSA2285 respectively to form Bax inhibitor-1 ihpRNA gene silencing vector, named as pGSA4285, containing sense and anti-sense BI-1 sequence which was spliced by chalcone synthase intron. Combined PCR identification and enzyme restriction analyses, the results showed that Bax inhibitor-1 ihpRNA gene silencing vector had been constructed and transferred into Agrobacterium tumefaciens EHA105 successfully, which laid a foundation for the further study on the function of BI-1 in plant PCD regulation.
文摘目的克隆人胰腺癌hedgehog信号通路中PTCH基因,构建PTCH基因表达载体并诱导融合蛋白表达。方法从人胰腺癌细胞株SW1990抽提总RNA,经RT-PCR扩增出PTCH基因,经纯化、回收目的基因PTCH,将其插入表达载体PET22b,转化E.coliBL21-CodonPlusTM-RP,构建重组质粒PET22b/PTCH,IPTG诱导表达融合蛋白,免疫印迹进行鉴定。结果从人胰腺癌细胞株SW1990克隆出长为789 bp PTCH目的片段,成功构建重组质粒PET22b/PTCH,并诱导表达目的蛋白。结论构建重组质粒PET22b/PTCH,并表达PTCH融合蛋白,为制备PTCH多克隆抗体打下良好基础。
