[Objective] The paper was to construct maize universal expression vector, in order to provide basis for using transgenic methods to improve abiotic stress tolerance of maize. [Method] Based on the transformation of ex...[Objective] The paper was to construct maize universal expression vector, in order to provide basis for using transgenic methods to improve abiotic stress tolerance of maize. [Method] Based on the transformation of existing pGreen0229 plant expression vector, phosphinothricin-resistant selectable marker-bar gene driving by CaMv35S promoter was constructed, which could be used to connect target gene of maize expression vector PGM-35Sbar, and transform Ji444 maize inbred lines by pollen tube pathway. [Result] The universal expression vector for PGM-35Sbar maize had been successfully constructed. When the maize plants were transformed, 14 resistant plants were obtained, and 12 plants were identified to be positive plants by PCR detection. [Conclusion] The study provided basis for rapid construction of maize expression vector containing specific target gene.展开更多
Based on an analysis of connotation and extension of the concept of the orthogonal curvilinear coordinates, we have deduced a platform of strain tensor expression of Cartesian coordinates, which turns out to be a func...Based on an analysis of connotation and extension of the concept of the orthogonal curvilinear coordinates, we have deduced a platform of strain tensor expression of Cartesian coordinates, which turns out to be a function of Lame coefficient and unit vector. By using transform matrix between Cartesian coordinates and orthogonal eurvilinear coordinates, we have deduced a mathematical expression for correcting displacement vector differential in orthogonal curvilinear coordinates, and given a general expression of strain tensor in orthogonal curvilinear coordinates.展开更多
基金Supported by National Transgenic Special Project(2009ZX08003-009B)Beijing Science and Technology Commission Project(KJCX201102003)~~
文摘[Objective] The paper was to construct maize universal expression vector, in order to provide basis for using transgenic methods to improve abiotic stress tolerance of maize. [Method] Based on the transformation of existing pGreen0229 plant expression vector, phosphinothricin-resistant selectable marker-bar gene driving by CaMv35S promoter was constructed, which could be used to connect target gene of maize expression vector PGM-35Sbar, and transform Ji444 maize inbred lines by pollen tube pathway. [Result] The universal expression vector for PGM-35Sbar maize had been successfully constructed. When the maize plants were transformed, 14 resistant plants were obtained, and 12 plants were identified to be positive plants by PCR detection. [Conclusion] The study provided basis for rapid construction of maize expression vector containing specific target gene.
文摘Based on an analysis of connotation and extension of the concept of the orthogonal curvilinear coordinates, we have deduced a platform of strain tensor expression of Cartesian coordinates, which turns out to be a function of Lame coefficient and unit vector. By using transform matrix between Cartesian coordinates and orthogonal eurvilinear coordinates, we have deduced a mathematical expression for correcting displacement vector differential in orthogonal curvilinear coordinates, and given a general expression of strain tensor in orthogonal curvilinear coordinates.
