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Novel adaptive IMEX two-step Runge-Kutta temporal discretization methods for unsteady flows
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作者 Xueyu QIN Jian YU +2 位作者 Xin ZHANG Zhenhua JIANG Chao YAN 《Chinese Journal of Aeronautics》 2025年第8期142-153,共12页
Efficient and accurate simulation of unsteady flow presents a significant challenge that needs to be overcome in computational fluid dynamics.Temporal discretization method plays a crucial role in the simulation of un... Efficient and accurate simulation of unsteady flow presents a significant challenge that needs to be overcome in computational fluid dynamics.Temporal discretization method plays a crucial role in the simulation of unsteady flows.To enhance computational efficiency,we propose the Implicit-Explicit Two-Step Runge-Kutta(IMEX-TSRK)time-stepping discretization methods for unsteady flows,and develop a novel adaptive algorithm that correctly partitions spatial regions to apply implicit or explicit methods.The novel adaptive IMEX-TSRK schemes effectively handle the numerical stiffness of the small grid size and improve computational efficiency.Compared to implicit and explicit Runge-Kutta(RK)schemes,the IMEX-TSRK methods achieve the same order of accuracy with fewer first derivative calculations.Numerical case tests demonstrate that the IMEX-TSRK methods maintain numerical stability while enhancing computational efficiency.Specifically,in high Reynolds number flows,the computational efficiency of the IMEX-TSRK methods surpasses that of explicit RK schemes by more than one order of magnitude,and that of implicit RK schemes several times over. 展开更多
关键词 Implicit-explicit temporal methods two-step Runge-Kutta methods Adaptive algorithm Unsteady flows Navier-Stokes equations
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Kinetic method for enzymatic analysis by predicting background with uricase reaction as model 被引量:7
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作者 廖飞 赵运胜 +4 位作者 赵利娜 陶佳 朱小云 王咏梅 左渝平 《Journal of Medical Colleges of PLA(China)》 CAS 2005年第6期338-344,共7页
Objective:To investigate the reliability for kinetic assay of substance with background predicted by the integrated method using uricase reaction as model. Methods: Absorbance before uricase action (Δ0) was estim... Objective:To investigate the reliability for kinetic assay of substance with background predicted by the integrated method using uricase reaction as model. Methods: Absorbance before uricase action (Δ0) was estimated by extrapolation with given lag time of steady-state reaction. With Km fixed at 12.5μmol/L, background absorbance (Δb) was predicted by nonlinearly fitting integrated Michaelis-Menten equation to Candida utilis uricase reaction curve. Uric acid in reaction solution was determined by the difference (ΔA) between Δ0 and Δb. Results .Ab usually showed deviation 〈3% from direct assay with residual substrate done fifth of initial substrate for analysis. ΔA showed CV 〈5% with resistance to common interferences except xanthine, and it linearly responded to uric acid with slope consistent to the absorptivity of uric acid. The lower limit was 2.0 μmol/L and upper limit reached 30 μmol/L in reaction solution with data monitored within 8 min reaction at 0. 015 U/ml uricase. Preliminary application to serum and urine gave better precision than the direct equilibrium method without the removal of proteins before analysis. Conclusion .This kinetic method with background predicted by the integrated method was reliable for enzymatic analysis, and it showed resistance to common interferences and enhanced efficiency at much lower cost. 展开更多
关键词 kinetic method enzymatic methods PREDICTION reaction curve fitting URICASE
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A Two-Step Modulus-Based Matrix Splitting Iteration Method Without Auxiliary Variables for Solving Vertical Linear Complementarity Problems 被引量:1
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作者 Hua Zheng Xiaoping Lu Seakweng Vong 《Communications on Applied Mathematics and Computation》 2024年第4期2475-2492,共18页
In this paper,a two-step iteration method is established which can be viewed as a generalization of the existing modulus-based methods for vertical linear complementarity problems given by He and Vong(Appl.Math.Lett.1... In this paper,a two-step iteration method is established which can be viewed as a generalization of the existing modulus-based methods for vertical linear complementarity problems given by He and Vong(Appl.Math.Lett.134:108344,2022).The convergence analysis of the proposed method is established,which can improve the existing results.Numerical examples show that the proposed method is efficient with the two-step technique. 展开更多
关键词 Vertical linear complementarity problem Modulus-based matrix splitting two-step method
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A new enzymatic method for determination of E. coli 被引量:1
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作者 Yun Xiang CI Tian Le LI(Department of Chemistry, Peking University. Beijing 100871) 《Chinese Chemical Letters》 SCIE CAS CSCD 1999年第10期849-850,共2页
beta-Galactosidase, a kind of endoenzyme in E. coli cells. can be released by the pore-forming action of colicin El. and E. coli can be detected rapidly by enzymatic method.
关键词 E-coli colicin E1 enzymatic method
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Ascorbic acid-induced porous iodide layer for a high-purity two-step solution-processed tin-lead mixed perovskite photodetector 被引量:2
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作者 Liansong Liu Fengren Cao +3 位作者 Liukang Bian Meng Wang Haoxuan Sun Liang Li 《Journal of Materials Science & Technology》 2025年第7期227-232,共6页
Tin(Sn)-lead(Pb)mixed halide perovskites have attracted widespread interest due to their wider re-sponse wavelength and lower toxicity than lead halide perovskites,Among the preparation methods,the two-step method mor... Tin(Sn)-lead(Pb)mixed halide perovskites have attracted widespread interest due to their wider re-sponse wavelength and lower toxicity than lead halide perovskites,Among the preparation methods,the two-step method more easily controls the crystallization rate and is suitable for preparing large-area per-ovskite devices.However,the residual low-conductivity iodide layer in the two-step method can affect carrier transport and device stability,and the different crystallization rates of Sn-and Pb-based per-ovskites may result in poor film quality.Therefore,Sn-Pb mixed perovskites are mainly prepared by a one-step method.Herein,a MAPb_(0.5)Sn_(0.5)I_(3)-based self-powered photodetector without a hole transport layer is fabricated by a two-step method.By adjusting the concentration of the ascorbic acid(AA)addi-tive,the final perovskite film exhibited a pure phase without residues,and the optimal device exhibited a high responsivity(0.276 A W^(-1)),large specific detectivity(2.38×10^(12) Jones),and enhanced stability.This enhancement is mainly attributed to the inhibition of Sn2+oxidation,the control of crystal growth,and the sufficient reaction between organic ammonium salts and bottom halides due to the AA-induced pore structure. 展开更多
关键词 PEROVSKITE SN-PB PHOTODETECTOR two-step solution method
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Aqueous Enzymatic Method to Extract Indigo Pigment from Baphicacanthus cusia Leaves 被引量:1
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作者 LIU Hui-lin HUANG Ran-lin +1 位作者 QU Chao LIU Shao 《Agricultural Science & Technology》 CAS 2022年第4期33-39,共7页
This study explored the extraction process of indigo pigment from Baphicacanthus cusia using aqueous enzymatic method for the first time. Through single factor tests and orthogonal tests, this study investigated the e... This study explored the extraction process of indigo pigment from Baphicacanthus cusia using aqueous enzymatic method for the first time. Through single factor tests and orthogonal tests, this study investigated the effects of extraction time, extraction temperature, enzyme concentration, pH value, and solidliquid ratio on the extraction rate of indigo pigment. The results showed that the extraction rate of indigo reached the highest(0.218%) when the solid-liquid ratio was 1:20, enzyme concentration was 6 g/L, pH value was 5, extraction temperature was 50℃, and extraction time was 3 h. The method had greatly shortened the extraction time, simplified the extraction process, and improved the extraction efficiency while producing little pollution and meeting the green environmental protection requirements. 展开更多
关键词 Baphicacanthus cusia Aqueous enzymatic method Indigo pigment Process optimization
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Synthesis of CCK-8 Tetrapeptide Fragment by Enzymatic Method
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作者 项光亚 Heiner Eckstein 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2003年第3期234-235,241,共3页
The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cholecystokinin C-terminal octapeptide CCK-8, was reported This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, T... The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cholecystokinin C-terminal octapeptide CCK-8, was reported This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, Trp-OMe and Met-OEt successively These three steps were catalyzed by α-chymotrpsin, Papain and α-chymotrpsin respectively The results of FAB-MS showed that all the products had the correct molecular mass 展开更多
关键词 CCK-8 enzymatic method α-chymotrpsin PAPAIN
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Analysis of Urea in Petfood Matrices: Comparison of Spectro-Colorimetric, Enzymatic and Liquid Chromatography Electrospray Ionization High Resolution Mass Spectrometry Methods
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作者 Patrick Pibarot Serge Pilard 《American Journal of Analytical Chemistry》 2012年第9期613-621,共9页
Adulteration may consist in non authorized source of nitrogen addition to increase the protein content of some raw materials. Urea which is authorized for feed is a non nutritional source of nitrogen in food and pet f... Adulteration may consist in non authorized source of nitrogen addition to increase the protein content of some raw materials. Urea which is authorized for feed is a non nutritional source of nitrogen in food and pet food. Adulteration of food or pet food raw material by urea is thus monitored by manufacturer and governmental authorities with official methods which are either enzymatic (Association of Official Agricultural Chemists, AOAC) or spectro-colorimetric (European Community, EC). Each method gives results which are not comparable and spectro-colorimetric methods may result in false-positive urea detection. Liquid chromatographic (LC/UV-DAD) analysis of extracts from spectro-colorimetric method indicates that presence of free amino-acid may interfere with colorimetric detection of urea in the EC method with pet food samples. Liquid chromatography electrospray ionization high resolution mass spectrometry (LC/ESI-HRMS) has allowed to quantify low content (<0.01%) of urea in pet food water extracts for samples which resulted in significant urea detection with colorimetric method and in content below the detection threshold with enzymatic method. This study demonstrates the EC colorimetric method is not applicable to pet food and also food samples which have a complex composition with significant levels of free amino acids. On the other hand we clearly evidenced by means of the LC/ESI-HRMS results that the AOC Enzymatic method is applicable to urea quantification in pet food samples and gives reliable results. 展开更多
关键词 Pet FOOD FOOD UREA ADULTERATION enzymatic method Spectro-Colorimetry LC/UV-DAD LC/ESI-HRMS
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Temperature-dependent photoluminescence on organic inorganic metal halide perovskite CH_3NH_3Pb I_(3-)Cl_ prepared on ZnO/FTO substrates using a two-step method 被引量:4
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作者 Shiwei Zhuang Deqian Xu +6 位作者 Jiaxin Xu Bin Wu Yuantao Zhang Xin Dong Guoxing Li Baolin Zhang Guotong Du 《Chinese Physics B》 SCIE EI CAS CSCD 2017年第1期482-487,共6页
Temperature-dependent photoluminescence characteristics of organic-inorganic halide perovskite CH3NH3Pb I3-xClx films prepared using a two-step method on ZnO/FTO substrates were investigated. Surface morphology and ab... Temperature-dependent photoluminescence characteristics of organic-inorganic halide perovskite CH3NH3Pb I3-xClx films prepared using a two-step method on ZnO/FTO substrates were investigated. Surface morphology and absorption characteristics of the films were also studied. Scanning electron microscopy revealed large crystals and substrate coverage. The orthorhombic-to-tetragonal phase transition temperature was-140 K. The films' exciton binding energy was 77.6 ± 10.9 meV and the energy of optical phonons was 38.8 ± 2.5 meV. These results suggest that perovskite CH3NH3Pb I(3-x)Clx films have excellent optoelectronic characteristics which further suggests their potential usage in perovskitebased optoelectronic devices. 展开更多
关键词 PEROVSKITE temperature-dependent photoluminescence two-step method ZNO
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Applying the Dynamic Two-Step Method to Forecast Remaining Oil Distribution of Lower Series ,Xiaermen Oilfield 被引量:2
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作者 周红 汤传意 李增辉 《Journal of China University of Geosciences》 SCIE CSCD 2006年第1期65-70,共6页
The distribution of remaining oil is often described qualitatively. The remaining oil distributed in the whole reservoir is calculated according to the characteristics of the space distribution of the saturation of re... The distribution of remaining oil is often described qualitatively. The remaining oil distributed in the whole reservoir is calculated according to the characteristics of the space distribution of the saturation of remaining oil. Logging data are required to accomplish this. However, many such projects cannot be completed. Since the old study of remaining oil distribution could not be quantified efficiently, the "dynamic two-step method" is presented. Firstly, the water cut of every flow unit in one well at one time is calculated according to the comprehensive water cut of a single well at one time. Secondly, the remaining oil saturation of the flow unit of the well at one time is calculated based on the water cut of the flow unit at a given time. The results show that "dynamic two-step method" has characteristics of simplicity and convenience, and is especially suitable for the study of remaining oil distribution at high water-cut stage. The distribution of remaining oil presented banding and potato form, remaining oil was relatively concentrated in faultage neighborhood and imperfect well netting position, and the net thickness of the place was great. This proposal can provide an effective way to forecast remaining oil distribution and enhance oil recovery, especially applied at the high water-cut stage. 展开更多
关键词 dynamic two-step method flow unit quantitative forecast remaining oil
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Enzymatic Aqueous Extraction of Soybean Oil 被引量:1
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作者 钱俊青 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2001年第2期212-216,共5页
The procedure of enzymatic aqueous extraction of soybean oil was assessed when two-step controlled enzymatic hydrolysis was applied. With aqueous extraction of soybean oil-containing protein, the highest yield of oil ... The procedure of enzymatic aqueous extraction of soybean oil was assessed when two-step controlled enzymatic hydrolysis was applied. With aqueous extraction of soybean oil-containing protein, the highest yield of oil was 96.1% at the optimized conditions studied. Soybean oil-containing protein was hydrolyzed and resulted in releasing part of oil. The separated protein that contained 40% oil was enriched due to its adsorption capacity of released oil, the average oil extraction yeild reached 93.5%. Then the high oil content protein was hydrolyzed again to release oil by enzyme, the oil extraction yeild was 80.4%. As a result, high quality of soybean oil was obtained and the content of total oil yield was 74.4%. 展开更多
关键词 soybean oil aqueous extraction enzymatic methods
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Preparation of ZnO/GO composite material with highly photocatalytic performance via an improved two-step method 被引量:4
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作者 Ya-Li Chen Chun-E.Zhang +3 位作者 Chao Deng Peng Fei Ming Zhong Bi-Tao Su 《Chinese Chemical Letters》 SCIE CAS CSCD 2013年第6期518-520,共3页
ZnO/graphene oxide(ZnO/GO) composite material,in which ZnO nanoparticles were densely coated on the GO nanosheets,was successfully prepared by an improved two-step method and characterized by IR, XRD,TEM,and UV-vis ... ZnO/graphene oxide(ZnO/GO) composite material,in which ZnO nanoparticles were densely coated on the GO nanosheets,was successfully prepared by an improved two-step method and characterized by IR, XRD,TEM,and UV-vis techniques.The improved photocatalytic property of the ZnO/GO composite material,evaluated by the photocatalytic degradation of methyl orange(MO) under UV irradiation,is ascribed to the intimate contact between ZnO and GO,the enhanced adsorption of MO,the quick electron transfer from excited ZnO particles to GO sheets and the activation of MO molecules viaπ-πinteraction between MO and GO. 展开更多
关键词 ZnO/GO composite material Improved two-step method Characterization Enhanced adsorptive and photocatalytic property
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Enzymatic Synthesis of a CCK-8 Tetrapeptide Fragment
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作者 GuangYaXIANG HeinerECKSTEIN 《Chinese Chemical Letters》 SCIE CAS CSCD 2004年第7期768-770,共3页
The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt is reported. It was synthesized by coupling Phac-Met-OEt with Gly-OMe ·HC1, Trp-OMe and Met-OEt successively, catalyzed by α-chymotrypsin, papai... The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt is reported. It was synthesized by coupling Phac-Met-OEt with Gly-OMe ·HC1, Trp-OMe and Met-OEt successively, catalyzed by α-chymotrypsin, papain and α-chymotrypsin respectively. The results of FAB-MS showed that the products had the correct molecular mass. 展开更多
关键词 CCK-8 enzymatic method Α-CHYMOTRYPSIN papain.
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The convergence ball and error analysis of the two-step Secant method
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作者 LIN Rong-fei WU Qing-biao +2 位作者 CHEN Min-hong KHAN Yasir LIU Lu 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2017年第4期397-406,共10页
Under the assumption that the nonlinear operator has Lipschitz continuous divided differences for the first order,we obtain an estimate of the radius of the convergence ball for the two-step secant method.Moreover,we ... Under the assumption that the nonlinear operator has Lipschitz continuous divided differences for the first order,we obtain an estimate of the radius of the convergence ball for the two-step secant method.Moreover,we also provide an error estimate that matches the convergence order of the two-step secant method.At last,we give an application of the proposed theorem. 展开更多
关键词 two-step secant method estimate of radius convergence ball Lipschitz continuous
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A new two-step variational model for multiplicative noise removal with applications to texture images
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作者 ZHANG Long-hui YAO Wen-juan +2 位作者 SHI Sheng-zhu GUO Zhi-chang ZHANG Da-zhi 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2024年第3期486-501,共16页
Multiplicative noise removal problems have attracted much attention in recent years.Unlike additive noise,multiplicative noise destroys almost all information of the original image,especially for texture images.Motiva... Multiplicative noise removal problems have attracted much attention in recent years.Unlike additive noise,multiplicative noise destroys almost all information of the original image,especially for texture images.Motivated by the TV-Stokes model,we propose a new two-step variational model to denoise the texture images corrupted by multiplicative noise with a good geometry explanation in this paper.In the first step,we convert the multiplicative denoising problem into an additive one by the logarithm transform and propagate the isophote directions in the tangential field smoothing.Once the isophote directions are constructed,an image is restored to fit the constructed directions in the second step.The existence and uniqueness of the solution to the variational problems are proved.In these two steps,we use the gradient descent method and construct finite difference schemes to solve the problems.Especially,the augmented Lagrangian method and the fast Fourier transform are adopted to accelerate the calculation.Experimental results show that the proposed model can remove the multiplicative noise efficiently and protect the texture well. 展开更多
关键词 multiplicative noise removal texture images total variation two-step variational method aug-mented Lagrangian method
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Solution of Nonlinear Integro Differential Equations by Two-Step Adomian Decomposition Method (TSAM)
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作者 Maryam Al-Mazmumy Safa O. Almuhalbedi 《International Journal of Modern Nonlinear Theory and Application》 2016年第4期248-255,共8页
The Adomian decomposition method (ADM) can be used to solve a wide range of problems and usually gets the solution in a series form. In this paper, we propose two-step Adomian Decomposition Method (TSAM) for nonlinear... The Adomian decomposition method (ADM) can be used to solve a wide range of problems and usually gets the solution in a series form. In this paper, we propose two-step Adomian Decomposition Method (TSAM) for nonlinear integro-differential equations that will facilitate the calculations. In this modification, compared to the standard Adomian decomposition method, the size of calculations was reduced. This modification also avoids computing Adomian polynomials. Numerical results are given to show the efficiency and performance of this method. 展开更多
关键词 Adomian Decomposition method Nonlinear Volterraintegro-Differential Equations Nonlinear Fredholmintegro-Differential Equations two-step
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超声辅助酶法提取咖啡果皮花青素工艺优化及其体外抗氧化活性评价 被引量:1
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作者 李学玲 李雪 +3 位作者 任四妹 许苑南 郭洁 杨莉 《中国酿造》 北大核心 2025年第2期251-255,共5页
为探究咖啡果皮花青素的提取工艺和体外抗氧化能力,该研究采用超声辅助酶法提取咖啡果皮花青素,通过单因素试验及正交试验对咖啡果皮花青素提取工艺进行优化,并研究其体外抗氧化活性。结果表明,咖啡果皮花青素最佳提取工艺条件为:提取温... 为探究咖啡果皮花青素的提取工艺和体外抗氧化能力,该研究采用超声辅助酶法提取咖啡果皮花青素,通过单因素试验及正交试验对咖啡果皮花青素提取工艺进行优化,并研究其体外抗氧化活性。结果表明,咖啡果皮花青素最佳提取工艺条件为:提取温度60℃、乙醇体积分数60%、果胶酶添加量2%、料液比1∶50(g∶mL)、提取时间40 min。在此优化条件下,咖啡果皮花青素得率达0.274%。体外抗氧化试验结果表明,当样品质量浓度为30μg/mL时,其对DPPH、OH自由基的清除率最佳,分别为91.40%、67.28%,半抑制浓度(IC50)值分别为6.36μg/mL、12.16μg/mL;当样品质量浓度为5μg/mL时,总还原能力最高(吸光度值为0.987)。因此,咖啡果皮花青素具有一定的抗氧化能力。 展开更多
关键词 咖啡果皮 花青素 超声辅助酶法 提取工艺 体外抗氧化活性
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灵芝多肽提取及冲剂制备工艺研究 被引量:1
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作者 林花 金海强 +5 位作者 姜银姬 金美玉 王婷 金星爱 尹花 车成来 《黑龙江农业科学》 2025年第3期54-60,共7页
为探明超声波辅助酶解法在提取灵芝多肽时的最佳工艺参数,促进灵芝多肽的提取及相应产品的工厂化生产,以多肽提取量为参考指标,进行灵芝多肽的提取及灵芝多肽冲剂制备工艺研究,首先进行单因素试验,随后采用正交试验设计来优化结合超声... 为探明超声波辅助酶解法在提取灵芝多肽时的最佳工艺参数,促进灵芝多肽的提取及相应产品的工厂化生产,以多肽提取量为参考指标,进行灵芝多肽的提取及灵芝多肽冲剂制备工艺研究,首先进行单因素试验,随后采用正交试验设计来优化结合超声波与酶法提取灵芝多肽的工艺条件,并进一步探究灵芝多肽冲剂的配方。结果表明,通过优化确定了灵芝多肽的最佳提取工艺参数,即料液比为1∶50,反应时间为2 h,反应温度为60℃,加酶量为6000 U·g^(-1)。此外,针对灵芝多肽冲剂的配方,也得出了最优比例,糊精占20%,淀粉占25%,柠檬酸占0.3%,蔗糖占6%。 展开更多
关键词 灵芝子实体 活性多肽 酶解法 提取 最佳条件
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半仿生联合提取技术在中药提取中的应用进展 被引量:1
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作者 刘玉峰 王佳玉 +2 位作者 李若凡 刘甜 周佳琦 《辽宁大学学报(自然科学版)》 2025年第2期152-158,共7页
中药有效成分复杂,如何最大程度地提取药效物质是提高中药疗效的核心与关键.中药提取技术的发展能够提高中药制剂的质量和稳定性,对中药产业的现代化和国际化具有重要意义.半仿生提取技术是一种模拟人胃肠吸收药物的仿生现代提取技术,... 中药有效成分复杂,如何最大程度地提取药效物质是提高中药疗效的核心与关键.中药提取技术的发展能够提高中药制剂的质量和稳定性,对中药产业的现代化和国际化具有重要意义.半仿生提取技术是一种模拟人胃肠吸收药物的仿生现代提取技术,可使药效物质更高效地被提取出来.随着科学技术的进步,半仿生提取技术也越来越成熟.将半仿生提取技术与酶法联合,具有提高微量成分提取效率等优势;将半仿生提取技术与超声波提取法联合,具有降低提取温度、提高有效成分稳定性等优势;将半仿生提取技术与微波提取法联合,具有选择性提取有效成分等优势.本文主要综述了近十年来3种主要半仿生联合提取技术在国内中药提取中的应用进展,旨在为半仿生提取技术在中药有效成分的提取与发现方面提供一定的思路. 展开更多
关键词 提取分离 半仿生-酶法 半仿生-超声波法 半仿生-微波法
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超声辅助酶法提取藤茶多糖工艺优化及抗氧化活性分析
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作者 尹团章 邵佩 +3 位作者 庄虎 陈浩 朱杰 何启强 《化学研究与应用》 北大核心 2025年第7期2005-2013,共9页
为优化藤茶多糖超声辅助提取工艺条件,并测定提取物的抗氧化活性。以恩施藤茶为原料,通过响应面法和超声波辅助酶法优化藤茶多糖的提取工艺条件,运用高效液相色谱-电喷雾检测(HPLC-CAD)法分析藤茶多糖的单糖组成,并研究其抗氧化活性。... 为优化藤茶多糖超声辅助提取工艺条件,并测定提取物的抗氧化活性。以恩施藤茶为原料,通过响应面法和超声波辅助酶法优化藤茶多糖的提取工艺条件,运用高效液相色谱-电喷雾检测(HPLC-CAD)法分析藤茶多糖的单糖组成,并研究其抗氧化活性。结果表明,藤茶多糖的最佳提取工艺条件为:料液比1∶15 g/mL,复合酶添加量3%,超声时间30 min,超声温度50℃,藤茶多糖得率达到12.01%±0.13%,藤茶多糖由6种单糖组成,其中蔗糖、葡萄糖含量较高。藤茶多糖具有较强的抗氧化活性,20 mg/mL的藤茶多糖对·OH的清除率为(89.12±0.21)%,对ABTS+·的清除率为(94.50±0.15)%。 展开更多
关键词 藤茶 多糖 超声辅助酶法提取 响应面 单糖组成 抗氧化活性
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