期刊文献+
共找到256篇文章
< 1 2 13 >
每页显示 20 50 100
Geo-engineered buffer capacity of two-layered absorbing system under the impact of rock avalanches based on Discrete Element Method 被引量:15
1
作者 BI Yu-zhang HE Si-ming +5 位作者 LI Xin-po WU Yong XU Qiang OUYANG Chao-jun SU Li-Jun WANG Hao 《Journal of Mountain Science》 SCIE CSCD 2016年第5期917-929,共13页
Many rock avalanches were triggered by the Wenchuan earthquake on May 12, 2008 in southwest China. Protection galleries covered with a single soil layer are usually used to protect against rockfall. Since one-layer pr... Many rock avalanches were triggered by the Wenchuan earthquake on May 12, 2008 in southwest China. Protection galleries covered with a single soil layer are usually used to protect against rockfall. Since one-layer protection galleries do not have sufficient buffer capacity, a two-layered absorbing system has been designed. This study aims to find whether an expanded poly-styrol (EPS) cushion, which is used in the soil-covered protection galleries for shock absorption, could be positioned under dynamic loadings. The dynamic impacts of the two-layered absorbing system under the conditions of rock avalanches are numerically simulated through a 2D discrete dement method. By selecting reasonable parameters, a series of numerical experiments were conducted to find the best combination for the two- layered absorbing system. The values of the EPS layer area as a percentage of the total area were set as 0% (Sl), 22~ (S2), and 70% ($3). 22~ of the area of the EPS layer was found to be a reasonable value, and experiments were conducted to find the best position of the EPS layer in the two-layered absorbing system. The numerical results yield useful conclusions regarding the interaction between the impacting avalanches and the two-layered absorbing system. The soil layer can absorb the shock energy effectively and S2 (0.4-m thick EPS cushion covered with soil layer) is the most efficient combination, which can reduce the impact force, compared with the other combinations. 展开更多
关键词 Discrete element method Buffer capacity Dynamic simulation Impact force two-layered absorbing system
原文传递
Two-layer cold storage method for pancreas and islet cell transplantation
2
作者 Yasuhiro Fujino 《World Journal of Gastroenterology》 SCIE CAS CSCD 2010年第26期3235-3238,共4页
The two-layer cold storage method (TLM) was f irst reported in 1988, consisting of a perfluorochemical (PFC) and initially Euro-Collins' solution, which was later replaced by University of Wisconsin solution (UW).... The two-layer cold storage method (TLM) was f irst reported in 1988, consisting of a perfluorochemical (PFC) and initially Euro-Collins' solution, which was later replaced by University of Wisconsin solution (UW). PFC is a biologically inert liquid and acts as an oxygen-supplying agent. A pancreas preserved using the TLM is oxygenated through the PFC and substrates are supplied by the UW solution. This allows the pancreas preserved using the TLM to generate adenosine triphosphate during storage, prolonging the preservation time. In a canine model, the TLM was shown to repair and resuscitate warm ischemically damaged pancreata during preservation, improve pancreas graft survival after transplantation, and also improve the islet yield after isolation. Clinical trials using the TLM in pancreas preservation before whole-pancreas transplantation and islet isolation have shown promising outcomes. We describe the role of the TLM in pancreas and islet transplantation. 展开更多
关键词 two-layer method Pancreas preservation Pancreas transplantation Islet transplantation Perfluorochemical
暂未订购
Green Functions with Pulsating Sources in A Two-Layer Fluid of Finite Depth 被引量:2
3
作者 MANYANGA D.O 段文洋 《China Ocean Engineering》 SCIE EI 2011年第4期609-624,共16页
The derivation of Green function in a two-layer fluid model has been treated in different ways. In a two-layer fluid with the upper layer having a free surface, there exist two modes of waves propagating due to the fr... The derivation of Green function in a two-layer fluid model has been treated in different ways. In a two-layer fluid with the upper layer having a free surface, there exist two modes of waves propagating due to the free surface and the interface. This paper is concerned with the derivation of Green functions in the three dimensional case of a stationary source oscillating. The source point is located either in the upper or lower part of a two-layer fluid of finite depth. The derivation is carried out by the method of singularities. This method has an advantage in that it involves representing the potential as a sum of singularities or multipoles placed within any structures being present. Furthermore, experience shows that the systems of equations resulted from using a singularity method possess excellent convergence characteristics and only a few equations are needed to obtain accurate numerical results. Validation is done by showing that the derived two-layer Green function can be reduced to that of a single layer of finite depth or that the upper Green function coincides with that of the lower, for each case. The effect of the density on the internal waves is demonstrated. Also, it is shown how the surface and internal wave amplitudes are compared for both the wave modes. The fluid in this case is considered to be inviscid and incompressible and the flow is irrotational. 展开更多
关键词 Green function two-layer fluid three dimensions singularities method
在线阅读 下载PDF
Electromagnetic scattering from two-layered rough interfaces with a PEC object:vertical polarization 被引量:1
4
作者 王安琪 郭立新 柴草 《Chinese Physics B》 SCIE EI CAS CSCD 2011年第5期23-32,共10页
Electromagnetic (EM) scattering from a stack of two rough interfaces separating a homogeneous medium with a perfectly electric conducting (PEC) object has been calculated through the method of moments for vertical... Electromagnetic (EM) scattering from a stack of two rough interfaces separating a homogeneous medium with a perfectly electric conducting (PEC) object has been calculated through the method of moments for vertical polarization. Theoretical formulations of EM scattering from multi-layered rough interfaces with a PEC object have been derived in detail and the total fields and their normal derivatives on the rough interfaces are solved. The two-layered model is a special case. In this work, a Gaussian rough surface was applied to simulate the rough interface. A cylinder was located above, between or below the two-layered rough interfaces. Through numerical simulations, the validity of this work is demonstrated by comparing it with existing scattering models, which are special cases that include a PEC object located above/below a single-layered rough interface and two-layered rough interfaces without an object. Subsequently, the influences of characteristic parameters, such as the relative permittivity of the medium, as well as the average height between the two rough surfaces, on the bistatic scattering coefficient are discussed. 展开更多
关键词 composite scattering two-layered rough interfaces electromagnetic wave scattering method of moments
原文传递
A baroclinic typhoon model with a moving multi-nested grid and variational adjustment initialisationI. Numerical method
5
作者 Wu Huiding Yang Xuelian Bai Shan and Li Guoqing 1. National Marine Environmental Forecasting Centre, State Oceanic Administration, Beijing 100081, China 2. Department of Physics and Physical Oceanography, Memorial University of Newfoundland, St. John’ s, 《Acta Oceanologica Sinica》 SCIE CAS CSCD 1999年第2期191-201,共11页
A baroclinic typhoon model with a moving multi--nested grid is applied in marine environmental forecasts. This paper describes the numerical methods of the model including governing equations, finite differencing, spl... A baroclinic typhoon model with a moving multi--nested grid is applied in marine environmental forecasts. This paper describes the numerical methods of the model including governing equations, finite differencing, split scheme and time integration. 展开更多
关键词 Typhoon model 3-dimensional baroclinic numerical method moving nested grid
在线阅读 下载PDF
RUMINATE METHOD-SOFTWARE PIPELINING ON NESTED LOOPS
6
作者 LEI WANG ZHIZHONGTANG and CHIHONG ZHANG(Dept. of Computer Science, Tsinghua Lirnivcrsitg Beijing 100084,P. R. China)(Final: wl,t ang ,zch@est4. dcs. tsinghua.edu. cn) 《Wuhan University Journal of Natural Sciences》 CAS 1996年第Z1期430-436,共7页
This paper offers a new method to solve the problem of software pipelininsr on nested loops. We first introduce our new software pipelininog method. Ruminate Method, which can optimize program with nested loops. We al... This paper offers a new method to solve the problem of software pipelininsr on nested loops. We first introduce our new software pipelininog method. Ruminate Method, which can optimize program with nested loops. We also outline an algorithm to realize it and introduce the hardware support we designed. The performance of Ruminate Method is analyzed at the end of this paper with the aid of our preliminary experimental result. 展开更多
关键词 Instruction-level Parallelism Software Pipeline Ruminate method nested Loop.
在线阅读 下载PDF
Characteristic analysis of scattering field in two-layer media by Green's function
7
作者 张萍 刘智颖 +2 位作者 阎守国 黄娟 张碧星 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第6期305-315,共11页
The problem of three-dimensional(3D) acoustic scattering in a complex medium has aroused considerable interest of researchers for many years. An ultrasonic scattered field calculating technique is proposed to study th... The problem of three-dimensional(3D) acoustic scattering in a complex medium has aroused considerable interest of researchers for many years. An ultrasonic scattered field calculating technique is proposed to study the scattering echo from strongly scattered materials in a two-layer medium in this work. Firstly, with the high frequency stationary phase method,the Green's function of two-layer fluid media is derived. And then based on the idea of integral equation discretization,the Green's function method is extended to two-layer fluid media to derive the scattering field expression of defects in a complex medium. With this method, the scattering field of 3D defect in a two-layer medium is calculated and the characteristics of received echoes are studied. The results show that this method is able to solve the scattering P wave field of 3D defect with arbitrary shape at any scattering intensity in two-layer media. Considering the circumstance of waterimmersion ultrasonic non-destructive test(NDT), the scattering sound field characteristics of different types of defects are analyzed by simulation, which will help to optimize the detection scheme and corresponding imaging method in practice so as to improve the detection quality. 展开更多
关键词 Green's function method two-layer media scattering field water-immersed detection
原文传递
A Multiple Nested Lattice Boltzmann Method and Its Application
8
作者 韩善灵 朱平 +1 位作者 来新民 林忠钦 《Journal of Shanghai Jiaotong university(Science)》 EI 2006年第1期71-76,92,共7页
The standard lattice Boltzmann method utilizes uniform grids to maintain a compact computational procedure. However, it is often less efficient to perform hydrodynamic and aerodynamic flow simulations when there is a ... The standard lattice Boltzmann method utilizes uniform grids to maintain a compact computational procedure. However, it is often less efficient to perform hydrodynamic and aerodynamic flow simulations when there is a need for high resolution. To resolve these difficulties, a multiple nested lattice Boltzmann method(MNLBM) was developed, which contains several overlapped layers with different resolutions in the computational domain. The data transference of flow field on two layers is accomplished by a Filippova procedure which is proved to satisfy the continuity of mass, momentum, and stresses across the interface. The proposed method is based on the standard lattice Boltzmann method, so it is easily performed.By numerical investigation, the result of present method has been agreed with that of literature, but the computation efficiency is higher than the standard lattice Boltzmann method. 展开更多
关键词 lattice Boltzmann method uniform grids multiple nested lattice Boltzmann method computational efficiency
在线阅读 下载PDF
A Similarity Technique for Solving Two-Layer Shallow-Water Equations
9
作者 Magda M. Kassem Medhat M. Helal +1 位作者 Mohammad L. Mekky Emad A. Mohamed 《Applied Mathematics》 2012年第4期315-321,共7页
This paper is devoted to the analysis of the two-layer shallow-water equations representing gravity currents. A similarity technique which is the characteristic function method is applied for this study. The applicati... This paper is devoted to the analysis of the two-layer shallow-water equations representing gravity currents. A similarity technique which is the characteristic function method is applied for this study. The application of the characteristic function method makes it possible to obtain the similarity forms depending on a group of infinitesimal transformations. Thus, the number of independent variables is reduced by one and the governing partial differential equations with the auxiliary conditions reduce to a system of ordinary differential equations with the appropriate auxiliary conditions. Numeric solutions are presented and discussed. 展开更多
关键词 The CHARACTERISTIC Function method The two-layer SHALLOW-WATER EQUATIONS GRAVITY CURRENTS
在线阅读 下载PDF
嵌套结构新方法计算二维六角离子晶体马德隆常数
10
作者 周群益 陈传盛 +2 位作者 周丽丽 莫云飞 刘天贵 《物理与工程》 2026年第2期101-106,共6页
马德隆常数能够反映晶体的物理特性,但用常规方法计算其值很烦琐。为了简化马德隆常数的计算方法,发展了单胞嵌套结构计算马德隆常数的新方法,并利用Matlab绘图说明了各级二维六角离子晶体单胞嵌套的形成过程和外层离子的分布规律。研... 马德隆常数能够反映晶体的物理特性,但用常规方法计算其值很烦琐。为了简化马德隆常数的计算方法,发展了单胞嵌套结构计算马德隆常数的新方法,并利用Matlab绘图说明了各级二维六角离子晶体单胞嵌套的形成过程和外层离子的分布规律。研究发现,根据晶体中的正负离子数,去除边界效应,证明了各级离子晶体是电中性的;同时,根据外层离子分布规律,推导出单胞嵌套-外埃夫琴法计算马德隆常数的递推公式,并利用迭代法计算了二维六角离子晶体多级马德隆常数,得到了一种计算晶体马德隆常数的简单方法。 展开更多
关键词 马德隆常数 嵌套结构 MATLAB 二维六角结构 埃夫琴法
在线阅读 下载PDF
猪瘟病毒RT-nested PCR检测方法的优化和应用 被引量:14
11
作者 朱小甫 张志 +2 位作者 李晓成 陈德坤 吴旭锦 《西北农林科技大学学报(自然科学版)》 CSCD 北大核心 2007年第6期11-14,共4页
为了优化猪瘟病毒的RT-nested PCR检测方法,对福建省猪瘟流行情况进行了调查。根据GenBank上发表的猪瘟病毒Shimen株基因序列设计并合成了2对引物,优化了猪瘟病毒(Classical swine fever virus,CSFV)的RT-nested PCR检测方法,并对所优化... 为了优化猪瘟病毒的RT-nested PCR检测方法,对福建省猪瘟流行情况进行了调查。根据GenBank上发表的猪瘟病毒Shimen株基因序列设计并合成了2对引物,优化了猪瘟病毒(Classical swine fever virus,CSFV)的RT-nested PCR检测方法,并对所优化的RT-nested PCR特异性进行了检验。结果表明,该方法检测CSFV cDNA含量的最低极限为1×10-7ng/mL,只有CSFV扩增出了272 bp的目的条带,从猪繁殖与呼吸综合征病毒(PRRSV)、猪流感病毒(SIV)、猪圆环病毒2型(PCV2)、猪伪狂犬病病毒(PRV)和猪细小病毒(PPV)阳性毒和健康猪脾、肝组织及阴性PK-15细胞均未扩增出特异性条带,说明该方法特异性强。应用此方法对福建省133份病料进行检测,结果有60份病料为阳性,阳性率为45.1%。结果提示优化的检测方法灵敏度高,特异性强;福建省猪群CSFV感染率高,需要加强CSF预防控制工作。 展开更多
关键词 猪瘟病毒 RT-nestedPCR 检测方法
在线阅读 下载PDF
猪细小病毒巢式PCR检测方法的建立
12
作者 郑佳 仇德洋 +2 位作者 曹志 姜莉群 吕延飞 《中国动物检疫》 2026年第3期89-94,共6页
为建立猪细小病毒(porcine parvovirus,PPV)的鉴别检测方法,针对编码PPV非结构蛋白1的基因(NS1)序列,分别设计了1对内套引物和1对外套引物,经优化反应条件,成功建立了PPV巢式PCR检测方法。该方法仅能特异性扩增PPV,对其他猪源病毒扩增... 为建立猪细小病毒(porcine parvovirus,PPV)的鉴别检测方法,针对编码PPV非结构蛋白1的基因(NS1)序列,分别设计了1对内套引物和1对外套引物,经优化反应条件,成功建立了PPV巢式PCR检测方法。该方法仅能特异性扩增PPV,对其他猪源病毒扩增均呈阴性,特异性好;对PPV重组质粒的检测限为5.60×10^(1) copies/μL,灵敏度高;组内和组间试验中,在同等扩增条件下,均出现同样的目的条带,重复性好。应用本研究建立的方法对76份临床样品进行检测,并将其检测结果与常规PCR进行比对,符合率为96.05%。结果表明,本研究建立的PPV巢式PCR检测方法灵敏度高、特异性强、重复性好,为PPV感染的临床检测和及时防控提供了技术支撑。 展开更多
关键词 巢式PCR 猪细小病毒 检测方法 NS1蛋白
在线阅读 下载PDF
基于嵌套组合模型的Web页面生成方法研究与应用
13
作者 彭龙江 《电子技术应用》 2026年第4期96-101,共6页
为了满足不同行业生产、运营、管理系统平台中用户角色多样、数据异构、页面展示需求复杂多变等需求,提出一种基于嵌套组合模型的Web页面生成方法。通过采用统一建模语言和面向对象编程技术,结合Vue.js和Nuxt.js框架,实现了页面元素的... 为了满足不同行业生产、运营、管理系统平台中用户角色多样、数据异构、页面展示需求复杂多变等需求,提出一种基于嵌套组合模型的Web页面生成方法。通过采用统一建模语言和面向对象编程技术,结合Vue.js和Nuxt.js框架,实现了页面元素的灵活配置和高效渲染。该方法在不同的工程项目中取得了满意的应用效果,显著降低了配置复杂度和维护成本,提升了用户体验,具有较好的应用推广价值。 展开更多
关键词 嵌套 组合 Web页面生成方法 多源异构数据 可配置页面
在线阅读 下载PDF
Numerical storm surge model with higher order finite difference method of lines for the coast of Bangladesh 被引量:2
14
作者 Gour Chandra Paul Md. Emran Ali 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2019年第6期100-116,共17页
In this study, the method of lines (MOLs) with higher order central difference approximation method coupled with the classical fourth order Runge-Kutta (RK(4,4)) method is used in solving shallow water equations (SWEs... In this study, the method of lines (MOLs) with higher order central difference approximation method coupled with the classical fourth order Runge-Kutta (RK(4,4)) method is used in solving shallow water equations (SWEs) in Cartesian coordinates to foresee water levels associated with a storm accurately along the coast of Bangladesh. In doing so, the partial derivatives of the SWEs with respect to the space variables were discretized with 5-point central difference, as a test case, to obtain a system of ordinary differential equations with time as an independent variable for every spatial grid point, which with initial conditions were solved by the RK(4,4) method. The complex land-sea interface and bottom topographic details were incorporated closely using nested schemes. The coastal and island boundaries were rectangularized through proper stair step representation, and the storing positions of the scalar and momentum variables were specified according to the rules of structured C-grid. A stable tidal regime was made over the model domain considering the effect of the major tidal constituent, M2 along the southern open boundary of the outermost parent scheme. The Meghna River fresh water discharge was taken into account for the inner most child scheme. To take into account the dynamic interaction of tide and surge, the generated tidal regime was introduced as the initial state of the sea, and the surge was then made to come over it through computer simulation. Numerical experiments were performed with the cyclone April 1991 to simulate water levels due to tide, surge, and their interaction at different stations along the coast of Bangladesh. Our computed results were found to compare reasonable well with the limited observed data obtained from Bangladesh Inland Water Transport Authority (BIWTA) and were found to be better in comparison with the results obtained through the regular finite difference method and the 3-point central difference MOLs coupled with the RK(4,4) method with regard to the root mean square error values. 展开更多
关键词 SHALLOW water equations method of lines higher order finite difference approximation method SURGE nested scheme
在线阅读 下载PDF
Numerical simulation of standing wave with 3D predictor-corrector finite difference method for potential flow equations 被引量:3
15
作者 罗志强 陈志敏 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 2013年第8期931-944,共14页
A three-dimensional (3D) predictor-corrector finite difference method for standing wave is developed. It is applied to solve the 3D nonlinear potential flow equa- tions with a free surface. The 3D irregular tank is ... A three-dimensional (3D) predictor-corrector finite difference method for standing wave is developed. It is applied to solve the 3D nonlinear potential flow equa- tions with a free surface. The 3D irregular tank is mapped onto a fixed cubic tank through the proper coordinate transform schemes. The cubic tank is distributed by the staggered meshgrid, and the staggered meshgrid is used to denote the variables of the flow field. The predictor-corrector finite difference method is given to develop the difference equa- tions of the dynamic boundary equation and kinematic boundary equation. Experimental results show that, using the finite difference method of the predictor-corrector scheme, the numerical solutions agree well with the published results. The wave profiles of the standing wave with different amplitudes and wave lengths are studied. The numerical solutions are also analyzed and presented graphically. 展开更多
关键词 three-dimensional (3D) nonlinear potential flow equation predictor-corrector finite difference method staggered grid nested iterative method 3D sloshing
在线阅读 下载PDF
Interaction of Linear Waves with Infinitely Long Horizontal Cylinders Studied by Boundary Element Method 被引量:2
16
作者 冯波 郑永红 +1 位作者 游亚戈 何再明 《China Ocean Engineering》 SCIE EI 2007年第2期317-330,共14页
The two-dimensional problems concerning the interaction of linear water waves with cylinders of arbitrary shape in two-layer deep water are investigated by use of the Boundary Integral Equation Method (BIEM). Simple... The two-dimensional problems concerning the interaction of linear water waves with cylinders of arbitrary shape in two-layer deep water are investigated by use of the Boundary Integral Equation Method (BIEM). Simpler new expressions for the Green functions are derived, and verified by comparison of results obtained by BIEM with those by an analytical method. Examined are the radiation and scattering of linear waves by two typical configurations of cylinders in two- layer deep water. Hydrodynamic behaviors including hydrodynamic coefficients, wave forces, reflection and transmission coef- ficients and energies are analyzed in detail, and some imeresting physical phenomena are observed. 展开更多
关键词 two-layer fluids boundary integral equation method hydrodynamic behavior
在线阅读 下载PDF
Nested Bethe Ansatz for Spin Ladder Model with Open Boundary Conditions 被引量:2
17
作者 WUJun-Fang ZHANGChun-Min +1 位作者 YUERui-Hong LIRun-Ling 《Communications in Theoretical Physics》 SCIE CAS CSCD 2005年第4期687-694,共8页
The nested Bethe ansatz (BA) method is applied to find the eigenvalues and the eigenvectors of the transfer matrix for spin-ladder model with open boundary conditions. Based on the reflection equation, we find the gen... The nested Bethe ansatz (BA) method is applied to find the eigenvalues and the eigenvectors of the transfer matrix for spin-ladder model with open boundary conditions. Based on the reflection equation, we find the general diagonal solution, which determines the generalboundary interaction in the Hamiltonian. We introduce the spin-ladder model with open boundary conditions. By finding the solution K± of the reflection equation which determines the nontrivial boundary terms in the Hamiltonian, we diagonalize the transfer matrix of the spin-ladder model with open boundary conditions in the framework of nested BA. 展开更多
关键词 integrable model coordinate bethe ansatz quantum inverse scattering method bethe ansatz equation nested bethe ansatz
在线阅读 下载PDF
Nested阵列宽带LFM信号二维DOA估计 被引量:1
18
作者 李小琳 张文俊 《上海大学学报(自然科学版)》 CAS CSCD 北大核心 2020年第4期506-517,共12页
Nested阵列以其大阵列孔径和更多自由度的优点,成为近期研究的热点。但大多集中在对窄带、平稳信号的波达方向(direction-of-arrival,DOA)估计,针对宽带信号,特别是非平稳宽带信号的研究较少。本工作基于分数阶傅里叶变换,应用DOA矩阵方... Nested阵列以其大阵列孔径和更多自由度的优点,成为近期研究的热点。但大多集中在对窄带、平稳信号的波达方向(direction-of-arrival,DOA)估计,针对宽带信号,特别是非平稳宽带信号的研究较少。本工作基于分数阶傅里叶变换,应用DOA矩阵方法,提出一种Nested阵列二维测角算法,不需进行二维非线性搜索和参数配对,可实现对宽带线性调频信号的空间波达角精确估计。仿真结果验证了该算法的优越性。 展开更多
关键词 nested阵列 分数阶傅里叶变换 矩阵法 宽带 波达方向估计
在线阅读 下载PDF
用nested-PCR方法快速检测鲑鱼肾杆菌 被引量:5
19
作者 刘荭 高隆英 +1 位作者 史秀杰 江育林 《水产学报》 CAS CSCD 北大核心 2002年第5期453-458,共6页
描述了用嵌套式聚合酶链式反应(nested PCR)快速检测鲑鱼细菌性肾病病原鲑鱼肾杆菌的方法。以BKDR和BKDF为引物,扩增鲑肾杆菌编码57kDa主要可溶性蛋白基因中501bp的DNA片段,再用引物BKDR2和BKDF2扩增其中长度为314bp的DNA片段。用其它1... 描述了用嵌套式聚合酶链式反应(nested PCR)快速检测鲑鱼细菌性肾病病原鲑鱼肾杆菌的方法。以BKDR和BKDF为引物,扩增鲑肾杆菌编码57kDa主要可溶性蛋白基因中501bp的DNA片段,再用引物BKDR2和BKDF2扩增其中长度为314bp的DNA片段。用其它15种常见鱼类致病菌验证这两组引物的特异性,结果没有非特异性的DNA片段被扩增出来。用酚抽提法和煮沸加冻融的方法获得的细菌裂解产物,PCR检测的灵敏度均可达到1.8×103CFU·mL-1,用Nested PCR进一步扩增PCR扩增的产物,检测灵敏度可再提高100倍。检测鲑鱼肾杆菌菌悬液与鲟卵的混合物,结果表明,该方法能准确、可靠、快速地检测鲑肾杆菌。 展开更多
关键词 nested-PCR方法 细菌性肾病 鲑鱼肾杆菌 嵌套式聚合酶链式反应 检测方法
在线阅读 下载PDF
Random failure mechanism method for assessment of working platform bearing capacity with a linear trend in undrained shear strength 被引量:1
20
作者 Marcin Chwaa Marek Kawa 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2021年第6期1513-1530,共18页
A bearing capacity evaluation for the surface strip foundation on a working platform modelled on a twolayered substrate is considered in the study.The upper layer is assumed as man-made and wellcontrolled and thus non... A bearing capacity evaluation for the surface strip foundation on a working platform modelled on a twolayered substrate is considered in the study.The upper layer is assumed as man-made and wellcontrolled and thus non-variable.The lower layer modelling natural cohesive soil is subjected to spatial variability of undrained shear strength.The random failure mechanism method(RFMM)is used to evaluate the bearing capacity.This approach employs a kinematic assessment of the critical load and incorporates the averaging of three-dimensional(3 D)random field along dissipation surfaces that result from the failure mechanism geometry.A novel version of the approach considering an additional linear trend of undrained shear strength in the spatially variable layer is proposed.The high efficiency of the RFMM algorithm is preserved.The influences of foundation length,trend slope in the spatially variable layer,fluctuation scales,and thickness of the homogenous sand layer on the resulting bearing capacity evaluations are analysed.Moreover,for selected cases,verification of the RFMM based assessment obtained using random finite difference method(RFDM)based on 3 D analysis is provided.Two types of analyses are performed using RFDM based on associated and non-associated flow rules.For associated flow rule which corresponds to RFMM,the RFMM is conservative and efficient and thus it seems preferable.However,if RFDM employs non-associated flow rule(much lower dilation angle for sand layer),the efficient RFMM is no longer conservative.For this situation,a combined approach that improves the efficiency of the numerical method is suggested. 展开更多
关键词 two-layered soil Random bearing capacity Fluctuation scale Kinematical approach Upper bound Random finite difference method(RFDM) Working platform
在线阅读 下载PDF
上一页 1 2 13 下一页 到第
使用帮助 返回顶部