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ZmZFP2 encoding a C4HC3-type RING zinc finger protein regulates kernel size and weight in maize
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作者 Long Zhang Qilei Wang +12 位作者 Wenyu Li Qi Zheng Miaomiao Fu Han Wang Xinyu Li Yan Wang Lingwei Hu Weigang Yao Lifeng Wang Huiyong Li Yongbin Dong Yongrui Wu Yuling Li 《The Crop Journal》 2025年第2期418-431,共14页
The development of maize(Zea mays)kernels is a complex physiological process regulated by numerous genes in a spatially and temporally coordinated manner.However,many regulatory genes involved in this process remain u... The development of maize(Zea mays)kernels is a complex physiological process regulated by numerous genes in a spatially and temporally coordinated manner.However,many regulatory genes involved in this process remain unidentified.In this study,we identified ZmZFP2,a gene encoding a C4HC3-type RING zinc finger protein,which regulates kernel size and weight.This discovery was based on suppression subtractive hybridization from maize endosperm in our previous research.We further investigated the role of ZmZFP2 in regulating kernel development.The zmzfp2-ems mutant exhibited significantly reduced kernel size and weight,accompanied by fewer endosperm cells and altered starch and protein accumulation.CRISPR/Cas9-mediated knockouts and overexpression lines confirmed that ZmZFP2 positively regulates kernel size and weight,with overexpression leading to increased kernel size and weight.Transcriptome analysis revealed that ZmZFP2 regulates genes involved in zeatin biosynthesis,starch metabolism,and protein processing,further supporting its role in kernel development.Additionally,ZmZFP2 was shown to interact with the transcription factor ZmEREB98,implicating it in the gene regulatory network during grain filling.Together,these findings demonstrate that ZmZFP2 is a key regulator of maize kernel size and weight,functioning through its E3 ubiquitin ligase activity and interactions with various metabolic pathways.This study provides novel insights into the genetic regulation of kernel development and presents potential strategies for improving maize yield and quality. 展开更多
关键词 MAIZE kernel weight and size Starch and protein accumulation E3 ubiquitin ligase Transcriptome analysis
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Maize kernel weight responses to sowing dateassociated variation in weather conditions 被引量:23
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作者 Baoyuan Zhou Yang Yue +3 位作者 Xuefang Sun Zaisong Ding Wei Ma Ming Zhao 《The Crop Journal》 SCIE CAS CSCD 2017年第1期43-51,共9页
Variation in weather conditions during grain filling has substantial effects on maize kernel weight(KW). The objective of this work was to characterize variation in KW with sowing date-associated weather conditions an... Variation in weather conditions during grain filling has substantial effects on maize kernel weight(KW). The objective of this work was to characterize variation in KW with sowing date-associated weather conditions and examine the relationship between KW, grain filling parameters, and weather factors. Maize was sown on eight sowing dates(SD) at 15–20-day intervals from mid-March to mid-July during 2012 and 2013 in the North China Plain. With sowing date delay, KW increased initially and later declined, and the greatest KW was obtained at SD6 in both years. The increased KW at SD6 was attributed mainly to kernel growth rate(Gmean), and effective grain-filling period(P). Variations in temperature and radiation were the primary factors that influenced KW and grain-filling parameters. When the effective cumulative temperature(AT) and radiation(Ra)during grain filling were 950 °C and 1005.4 MJ m-2, respectively, P and KW were greatest. High temperatures(daily maximum temperature [Tmax] > 30.2 °C) during grain filling under early sowing conditions, or low temperatures(daily minimum temperature [Tmin] < 20.7 °C) under late sowing conditions combined with high diurnal temperature range(Tmax-min> 7.1 °C) decreased kernel growth rate and ultimately final KW. When sowing was performed from May 25 through June 27, higher KW and yield of maize were obtained. We conclude that variations in environmental conditions(temperature and radiation) during grain filling markedly affect growth rate and duration of grain filling and eventually affect kernel weight and yield of maize. 展开更多
关键词 MAIZE Sowing date Weather conditions kernel weight Grain filling
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The Boundedness of Littlewood-Paley Operators with Rough Kernels on Weighted (L^q , L^p )~α (R^n) Spaces 被引量:5
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作者 Ximei Wei Shuangping Tao 《Analysis in Theory and Applications》 2013年第2期135-148,共14页
In this paper, we shall deal with the boundedness of the Littlewood-Paley operators with rough kernel. We prove the boundedness of the Lusin-area integral μΩs and Littlewood-Paley functions μΩ and μλ^* on the w... In this paper, we shall deal with the boundedness of the Littlewood-Paley operators with rough kernel. We prove the boundedness of the Lusin-area integral μΩs and Littlewood-Paley functions μΩ and μλ^* on the weighted amalgam spaces (Lω^q,L^p)^α(R^n)as 1〈q≤α〈p≤∞. 展开更多
关键词 Littlewood-Paley operator weighted amalgam space rough kernel Ap weight.
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Commutators of Weighted Lipschitz Functions and Multilinear Singular Integrals with Non-Smooth Kernels 被引量:3
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作者 LIAN Jia-li MA Bo-lin WU Huo-xiong 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2011年第3期353-367,共15页
This paper is devoted to studying the commutators of the multilinear singular integral operators with the non-smooth kernels and the weighted Lipschitz functions. Some mapping properties for two types of commutators o... This paper is devoted to studying the commutators of the multilinear singular integral operators with the non-smooth kernels and the weighted Lipschitz functions. Some mapping properties for two types of commutators on the weighted Lebesgue spaces, which extend and generalize some previous results, are obtained. 展开更多
关键词 COMMUTATOR multilinear singular integral non-smooth kernel weighted Lipschitz function weights.
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A Multi-model Approach for Soft Sensor Development Based on Feature Extraction Using Weighted Kernel Fisher Criterion 被引量:6
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作者 吕业 杨慧中 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2014年第2期146-152,共7页
Multi-model approach can significantly improve the prediction performance of soft sensors in the process with multiple operational conditions.However,traditional clustering algorithms may result in overlapping phenome... Multi-model approach can significantly improve the prediction performance of soft sensors in the process with multiple operational conditions.However,traditional clustering algorithms may result in overlapping phenomenon in subclasses,so that edge classes and outliers cannot be effectively dealt with and the modeling result is not satisfactory.In order to solve these problems,a new feature extraction method based on weighted kernel Fisher criterion is presented to improve the clustering accuracy,in which feature mapping is adopted to bring the edge classes and outliers closer to other normal subclasses.Furthermore,the classified data are used to develop a multiple model based on support vector machine.The proposed method is applied to a bisphenol A production process for prediction of the quality index.The simulation results demonstrate its ability in improving the data classification and the prediction performance of the soft sensor. 展开更多
关键词 feature extraction weighted kernel Fisher criterion CLASSIFICATION soft sensor
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Identification of loci and candidate genes controlling kernel weight in barley based on a population for which whole genome assemblies are available for both parents 被引量:2
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作者 Hong Zhou Wei Luo +6 位作者 Shang Gao Jian Ma Meixue Zhou Yuming Wei Youliang Zheng Yaxi Liu Chunji Liu 《The Crop Journal》 SCIE CSCD 2021年第4期854-861,共8页
Kernel weight(KW), together with kernel number per unit area, determines yield of cereal crops. Here,two barley recombinant inbred lines(RILs) populations with a shared parent were used to identify loci controlling KW... Kernel weight(KW), together with kernel number per unit area, determines yield of cereal crops. Here,two barley recombinant inbred lines(RILs) populations with a shared parent were used to identify loci controlling KW. One is Baudin/AWCS276(BA) for which a linkage map was available. Several largeeffect QTL controlling KW were detected in this population. Another is Morex/AWCS276(MA). A linkage map with 5273 makers formed 1454 clusters, was constructed by the genotyping by sequence(GBS) data of 201 RILs from this population. A single marker was selected to represent each of the clusters and the linkage map constructed with these markers covers a total length of 1022.4 c M with an average interval of approximately 0.7 cM between loci. Three of the large-effect loci controlling KW(located on 2 HL, 6 HL,and 7 HL, respectively) identified from the BA population were also detected in the MA population under different environments. The locus on 6 HL was detected in each of the experiments conducted for both populations thus was selected for developing near isogenic lines(NILs). Apart from KW, the two isolines for each pair of the putative NILs obtained showed no significant difference for any of the morphological characteristics assessed. The average difference in KW between the isolines for all the NILs obtained was about 15% based on assessments under both glasshouse and field conditions. Taken advantage that high quality genome assemblies for both Morex and AWCS276 are available, we identified candidate genes underlying two of the three loci based on an orthologous analysis. The NILs developed and the candidate genes identified in this study should facilitate the cloning and functional analysis of genes regulating KW in barley. 展开更多
关键词 BARLEY kernel weight Near isogenic lines AWCS276
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Toeplitz Type Operator Associated to Singular Integral Operator with Variable Kernel on Weighted Morrey Spaces 被引量:1
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作者 Yuexiang He Yueshan Wang 《Analysis in Theory and Applications》 CSCD 2016年第1期90-102,共13页
Suppose T^k,l and T^k,2 are singular integrals with variable kernels and mixed homogeneity or ±I (the identity operator). Denote the Toeplitz type operator by T^b=k=1∑^QT^k,1M^bT^k,2 where M^bf= bf. In this pa... Suppose T^k,l and T^k,2 are singular integrals with variable kernels and mixed homogeneity or ±I (the identity operator). Denote the Toeplitz type operator by T^b=k=1∑^QT^k,1M^bT^k,2 where M^bf= bf. In this paper, the boundedness of Tb on weighted Morrey space are obtained when b belongs to the weighted Lipschitz function space and weighted BMO function space, respectively. 展开更多
关键词 Toeplitz type operator singular integral operator variable Calderon-Zygmund kernel weighted BMO function weighted Lipschitz function weighted Morrey space.
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Boundedness for the Singular Integral with Variable Kernel and Fractional Differentiation on Weighted Morrey Spaces 被引量:1
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作者 Chao Xue Kai Zhu Yanping Chen 《Analysis in Theory and Applications》 CSCD 2016年第3期205-214,共10页
Let T be the singular integral operator with variable kernel, T* be the adjoint of T and T# be the pseudo-adjoint of T. Let TIT2 be the product of T1 and T2, T1 o T2 be the pseudo product of T1 and T2. In this paper,... Let T be the singular integral operator with variable kernel, T* be the adjoint of T and T# be the pseudo-adjoint of T. Let TIT2 be the product of T1 and T2, T1 o T2 be the pseudo product of T1 and T2. In this paper, we establish the boundedness for commutators of these operators and the fractional differentiation operator D^γ on the weighted Morrey spaces. 展开更多
关键词 Singular integral variable kernel fractional differentiation BMO Sobolev space weighted Morrey spaces
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A Full-Newton Step Feasible Interior-Point Algorithm for the Special Weighted Linear Complementarity Problems Based on a Kernel Function 被引量:2
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作者 GENG Jie ZHANG Mingwang ZHU Dechun 《Wuhan University Journal of Natural Sciences》 CAS CSCD 2024年第1期29-37,共9页
In this paper,a new full-Newton step primal-dual interior-point algorithm for solving the special weighted linear complementarity problem is designed and analyzed.The algorithm employs a kernel function with a linear ... In this paper,a new full-Newton step primal-dual interior-point algorithm for solving the special weighted linear complementarity problem is designed and analyzed.The algorithm employs a kernel function with a linear growth term to derive the search direction,and by introducing new technical results and selecting suitable parameters,we prove that the iteration bound of the algorithm is as good as best-known polynomial complexity of interior-point methods.Furthermore,numerical results illustrate the efficiency of the proposed method. 展开更多
关键词 interior-point algorithm weighted linear complementarity problem full-Newton step kernel function iteration complexity
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The Boundedness of the Singular Integral Operator with Variable Calder′on-Zygmund Kernel on Weighted Morrey Spaces 被引量:1
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作者 潘亚丽 李昌文 温宗良 《Chinese Quarterly Journal of Mathematics》 2015年第1期39-46,共8页
In this paper, we will study the boundedness of the singular integral operator with variable Calder′on-Zygmund kernel on the weighted Morrey spaces Lp,κ(ω) for q′≤ p < ∞and 0 < κ < 1. Furthermore, the ... In this paper, we will study the boundedness of the singular integral operator with variable Calder′on-Zygmund kernel on the weighted Morrey spaces Lp,κ(ω) for q′≤ p < ∞and 0 < κ < 1. Furthermore, the boundedness for the commutator with BMO functions is also obtained. 展开更多
关键词 variable Calder′on-Zygmund kernels weighted Morrey spaces BMO
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Toeplitz Operator Related to Singular Integral with Non-Smooth Kernel on Weighted Morrey Space
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作者 Yuexiang He 《Analysis in Theory and Applications》 CSCD 2017年第3期240-252,共13页
Let T1 be a singular integral with non-smooth kernel or ±I, let T2 and T4 be the linear operators and let T3 = ± I. Denote the Toeplitz type operator by Tb = T1M^b Ia T2 + T3IaMbT4,where M^bf = bf, and Ib i... Let T1 be a singular integral with non-smooth kernel or ±I, let T2 and T4 be the linear operators and let T3 = ± I. Denote the Toeplitz type operator by Tb = T1M^b Ia T2 + T3IaMbT4,where M^bf = bf, and Ib is the fractional integral operator. In this paper, we investigate the boundedness of the operator Tb on the weighted Morrey space when b belongs to the weighted BMO space. 展开更多
关键词 Toeplitz operator non-smooth kernel weighted BMO fractional integral weighted Morrey space.
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Weighted estimates for strongly singular integral operators with rough kernels
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作者 黄文礼 陶祥兴 李胜宏 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 2010年第6期761-768,共8页
The Fourier transform and the Littlewood-Paley theory are used to give the weighted boundedness of a strongly singular integral operator defined in this paper. The paper shows that the strongly singular integral opera... The Fourier transform and the Littlewood-Paley theory are used to give the weighted boundedness of a strongly singular integral operator defined in this paper. The paper shows that the strongly singular integral operator is bounded from the Sobolev space to the Lebesgue space. 展开更多
关键词 strongly singular integral operators rough kernels Ap weights
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Comparison of Uniform and Kernel Gaussian Weight Matrix in Generalized Spatial Panel Data Model
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作者 Tuti Purwaningsih Erfiani   《Open Journal of Statistics》 2015年第1期90-95,共6页
Panel data combine cross-section data and time series data. If the cross-section is locations, there is a need to check the correlation among locations. ρ and λ are parameters in generalized spatial model to cover e... Panel data combine cross-section data and time series data. If the cross-section is locations, there is a need to check the correlation among locations. ρ and λ are parameters in generalized spatial model to cover effect of correlation between locations. Value of ρ or λ will influence the goodness of fit model, so it is important to make parameter estimation. The effect of another location is covered by making contiguity matrix until it gets spatial weighted matrix (W). There are some types of W—uniform W, binary W, kernel Gaussian W and some W from real case of economics condition or transportation condition from locations. This study is aimed to compare uniform W and kernel Gaussian W in spatial panel data model using RMSE value. The result of analysis showed that uniform weight had RMSE value less than kernel Gaussian model. Uniform W had stabil value for all the combinations. 展开更多
关键词 Component UNIFORM weight kernel GAUSSIAN weight GENERALIZED Spatial PANEL Data Model
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LKAW: A Robust Watermarking Method Based on Large Kernel Convolution and Adaptive Weight Assignment
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作者 Xiaorui Zhang Rui Jiang +3 位作者 Wei Sun Aiguo Song Xindong Wei Ruohan Meng 《Computers, Materials & Continua》 SCIE EI 2023年第4期1-17,共17页
Robust watermarking requires finding invariant features under multiple attacks to ensure correct extraction.Deep learning has extremely powerful in extracting features,and watermarking algorithms based on deep learnin... Robust watermarking requires finding invariant features under multiple attacks to ensure correct extraction.Deep learning has extremely powerful in extracting features,and watermarking algorithms based on deep learning have attracted widespread attention.Most existing methods use 3×3 small kernel convolution to extract image features and embed the watermarking.However,the effective perception fields for small kernel convolution are extremely confined,so the pixels that each watermarking can affect are restricted,thus limiting the performance of the watermarking.To address these problems,we propose a watermarking network based on large kernel convolution and adaptive weight assignment for loss functions.It uses large-kernel depth-wise convolution to extract features for learning large-scale image information and subsequently projects the watermarking into a highdimensional space by 1×1 convolution to achieve adaptability in the channel dimension.Subsequently,the modification of the embedded watermarking on the cover image is extended to more pixels.Because the magnitude and convergence rates of each loss function are different,an adaptive loss weight assignment strategy is proposed to make theweights participate in the network training together and adjust theweight dynamically.Further,a high-frequency wavelet loss is proposed,by which the watermarking is restricted to only the low-frequency wavelet sub-bands,thereby enhancing the robustness of watermarking against image compression.The experimental results show that the peak signal-to-noise ratio(PSNR)of the encoded image reaches 40.12,the structural similarity(SSIM)reaches 0.9721,and the watermarking has good robustness against various types of noise. 展开更多
关键词 Robust watermarking large kernel convolution adaptive loss weights high-frequency wavelet loss deep learning
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Rapid Determination of Seed Number and Thousand Kernel Weight of Asparagus officinalis L.Using Image Processing Method
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作者 Huimin GAO Yu TIAN +1 位作者 Zhiqiang LIU Yanpo CAO 《Asian Agricultural Research》 2019年第11期53-55,共3页
Asparagus officinalis L.is favored by its high health function,but its hybrid seeds are expensive.The amount of seed,seed plumpness and germination rate are related to the production costs of breeding enterprises and ... Asparagus officinalis L.is favored by its high health function,but its hybrid seeds are expensive.The amount of seed,seed plumpness and germination rate are related to the production costs of breeding enterprises and large growers.Therefore,it is necessary to investigate the seed number and thousand kernel weight of A.officinalis L.This study developed a quick and accurate method to measure the seed number and thousand kernel weight of A.officinalis L.using image processing technology.Seed sample of A.officinalis L.was scanned with 200 dpi resolution,and the seed number was then obtained using Image-ProPlus software.After weighing the seeds,thousand kernel weight was finally calculated.By recording‘macro’,the batch processing of the samples can also be realized.This method is simple and accurate,and can greatly save the time of investigation. 展开更多
关键词 ASPARAGUS OFFICINALIS L. SEED number Thousand kernel weight Image processing method
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2-complex Symmetric Weighted Composition Operators on Fock Space of C^(N)
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作者 JIANG Zhijie 《数学进展》 北大核心 2025年第1期37-49,共13页
The aim of the present paper is to study 2-complex symmetric bounded weighted composition operators on the Fock space of C^(N) with the conjugations J and J_(t,A,b) defined by ■ respectively,where k(z_(1),...,z_N)=(... The aim of the present paper is to study 2-complex symmetric bounded weighted composition operators on the Fock space of C^(N) with the conjugations J and J_(t,A,b) defined by ■ respectively,where k(z_(1),...,z_N)=(■,...,■),t∈C,b∈C^(N) and A is a linear operator on C^(N).An example of 2-complex symmetric bounded weighted composition operator with the conjugation J_(t,A,b) is given. 展开更多
关键词 Fock space weighted composition operator 2-complex symmetric operator reproducing kernel function
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AN EXTENSION OF MARCINKIEWICZ INTEGRALS WITH ROUGH KERNELS
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作者 Huoxiong WU Lin WU 《Acta Mathematica Scientia》 2025年第3期789-808,共20页
This paper considers the following Marcinkiewicz type integrals■which can be regarded as an extension of the classical Marcinkiewicz integral po introduced by Stein in[Trans Amer Math Soc,88(1958):159-172],where Ω i... This paper considers the following Marcinkiewicz type integrals■which can be regarded as an extension of the classical Marcinkiewicz integral po introduced by Stein in[Trans Amer Math Soc,88(1958):159-172],where Ω is a homogeneous function of degree zero on R^(n)with mean value zero in the unit sphere S^(n-1),Under the assumption that Ω∈L^(∞)(S^(n-1)),the authors establish the L^(q)-estimate and weak(1,1)type estimate as well as the corresponding weighted estimates for po.s with 1<q<∞ and 0<β(q-1)n/q.Moreover,the bounds do not depend on β and the strong(q,q)type and weak(1,1)type estimates for the classical Marcinkiewicz integral po can be recovered from the above estimates of μΩ,β whenβ→0. 展开更多
关键词 Marcinkiewicz integral rough kernel uniform estimates A_(p)weight
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县域城乡融合水平测度及时空演变——基于国家城乡融合发展试验区典型区县数据
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作者 陈中伟 赫苒 《南都学坛》 2026年第2期100-113,共14页
城乡融合发展是实现乡村振兴与新型城镇化协同推进的关键路径。以首批国家城乡融合发展试验区中的35个典型区县为研究对象,构建包含人口、经济、空间、社会和生态五大维度的测度体系,采用熵权TOPSIS法、核密度估计、泰尔系数及Dagum基... 城乡融合发展是实现乡村振兴与新型城镇化协同推进的关键路径。以首批国家城乡融合发展试验区中的35个典型区县为研究对象,构建包含人口、经济、空间、社会和生态五大维度的测度体系,采用熵权TOPSIS法、核密度估计、泰尔系数及Dagum基尼系数分解法,分析2013—2022年县域城乡融合发展水平的时空特征与区域差异,探究政策影响。研究发现:(1)全国城乡融合发展水平呈“阶梯式上升—结构调整—韧性修复”的演进轨迹,经济融合为核心驱动力,空间融合、生态融合提供增长动力;(2)区域差异呈“东高西低”的梯度格局,东部地区得分均值领先且政策创新成效显著,中部地区以1.7%的年均增速呈现追赶态势,西部地区依托生态补偿与数字基建实现局部突破,且东中西部存在维度异质性短板;(3)城乡融合以“五维联动”驱动质量跃升。经济联动协同、人口流动畅通、服务均衡共享、生态短板补齐、空间互联互通,各维度协同推进城乡融合发展进程;(4)差异来源呈波浪式收敛,省际行政壁垒导致的要素流动障碍仍是主要制约,而省内差异因精准施策显著压缩。研究表明,需通过分区域精准补短板(东部生态协同、中部基建产业赋能、西部综合提升)、强化省际政策协同、优化要素跨域配置,破解城乡二元结构,推动区域均衡发展。 展开更多
关键词 县域城乡融合 熵权TOPSIS 核密度估计
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Dry Matter Accumulation,Moisture Content in Maize Kernel and Their Influences on Mechanical Harvesting 被引量:18
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作者 阮龙 王俊 +4 位作者 陈义红 王世济 张玮 左晓龙 陈洪俭 《Agricultural Science & Technology》 CAS 2011年第12期1857-1860,共4页
[Objective] The aim of this study was to investigate the dry matter accumulation,moisture content in maize kernel and their influences on mechanical harvesting.[Methods] Using Zhengdan 958,Xianyu 335,Hongda 8 and Liyu... [Objective] The aim of this study was to investigate the dry matter accumulation,moisture content in maize kernel and their influences on mechanical harvesting.[Methods] Using Zhengdan 958,Xianyu 335,Hongda 8 and Liyu 16 as experimental materials,we preliminarily measured the dynamic changes of dry matter accumulation and moisture content with the days after pollination lasted,and investigated the effect of moisture content in kernels on mechanical harvesting of Zhengdan 958 and Liyu 16.[Results] During summer growing season,Zhengdan 958 and Liyu 16 became physiologically mature and suitable for harvesting at about 51 days after pollination,Xianyu 335 and Hongda 8 required 58 days to become physiologically mature and suitable for harvesting after pollination.At physiologically mature stage,the moisture contents in kernels of Zhengdan 958,Xianyu 335,Hongda 8 and Liyu 16 were respectively 33.74%,28.86%,32.05% and 35.24%,respectively.Adopting mechanical harvesting at physiologically mature stage,the kernel loss rate of Zhengdan 958 and Liyu 16 was consistent with the range of mechanical operation index(kernel loss rate of 0.84%≤2%),while the percentage of broken kernels did not accord with the range of mechanical operation index(kernel crashing rate of 10.12%≧1%).The results indicate that Zhengdan 958 and Liyu 16 are not suitable for mechanical harvesting using full feeding combine havester.[Conclusion] This study provided theoretical basis for improving the maize harvest index under delayed harvesting. 展开更多
关键词 Maize(Zea mays) kernel weight Moisture content Physiological mature stage
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ZmARFTF2对玉米籽粒大小及淀粉类型的调控
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作者 王艳丽 张锴 +3 位作者 张勇 温晓蕾 张敏 张萌 《核农学报》 北大核心 2026年第1期36-44,共9页
生长素响应因子(ARF)是作物特有的一类生长素转录因子,在生长素代谢通路上调控植物的生长发育。为验证ARF转录因子在玉米籽粒发育过程中的作用,本研究克隆获得ZmARFTF2(Zm00001eb045640)基因,生物信息学分析显示,该基因位于玉米基因组1... 生长素响应因子(ARF)是作物特有的一类生长素转录因子,在生长素代谢通路上调控植物的生长发育。为验证ARF转录因子在玉米籽粒发育过程中的作用,本研究克隆获得ZmARFTF2(Zm00001eb045640)基因,生物信息学分析显示,该基因位于玉米基因组1号染色体,编码序列全长为2076 bp,编码691个氨基酸,ZmARFTF2蛋白具有跨膜结构,等电点为7.92。ZmARFTF2具有B3亚家族和Auxin_resp亚家族特有的保守结构域。对该基因启动子上游2 kb序列进行分析,发现启动子区域含有与ABA合成途径、干旱诱导、赤霉素响应、参与胚乳表达等相关的顺式作用元件。对玉米ARF基因家族构建系统发育树,发现ZmARFTF2与ZmARF17亲缘关系最近。ZmARFTF2基因以B73为背景的甲基磺酸乙酯(EMS)突变体EMS3-0a1dd2中单碱基突变造成终止密码子提前。与野生型玉米自交系B73相比,纯合突变体EMS3-0a1dd2籽粒长度、籽粒宽度、籽粒厚度、百粒重、容重显著增加;突变体中直链淀粉含量显著高于野生型B73,突变体中支链淀粉含量显著低于野生型B73。ZmARFTF2基因功能的丧失导致了玉米自交系B73由马齿型转变成硬粒型。过表达ZmARFTF2基因拟南芥的籽粒、果荚显著小于野生型拟南芥。ZmARFTF2负向调控玉米籽粒大小和百粒重,影响玉米淀粉合成途径。本研究结果为解析玉米籽粒性状和品质性状提供了基因资源。 展开更多
关键词 玉米 籽粒大小 百粒重 直链淀粉 支链淀粉
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