Thinopyrum ponticum(2n=10×=70),a wild relative of common wheat(Triticum aestivum L.),is considered an invaluable genetic resource for wheat improvement due to its abundance of genes conferring resistance to bioti...Thinopyrum ponticum(2n=10×=70),a wild relative of common wheat(Triticum aestivum L.),is considered an invaluable genetic resource for wheat improvement due to its abundance of genes conferring resistance to biotic and abiotic stresses.This study focused on the CH97 line,derived from the BC1F7 progeny of a cross between wheat cv.7182 and Th.ponticum.Cytological evidence showed that CH97 has 42 chromosomes,forming 21 bivalents at meiotic metaphase I,with the bivalents subsequently separating and moving to opposite poles during meiotic anaphase I.Through a combination of fluorescence in situ hybridization(FISH),genomic in situ hybridization(GISH),multicolor GISH(mc-GISH),and liquid array analysis,it was determined that CH97 comprises 40 wheat chromosomes and two alien chromosomes from the Ee genome of Th.ponticum,featuring the absence of a pair of 5D chromosomes and variations in 1B,6B,and 7B chromosomes.These findings confirm that CH97 is a stable wheat-Th.ponticum 5E(5D)alien disomic substitution line.Inoculation experiments revealed that CH97 exhibits high resistance to wheat powdery mildew and stripe rust throughout the growth period,in contrast to the highly susceptible common wheat parent 7182.Compared to 7182,CH97 displayed improvements in thousand-kernel weight and kernel length.Additionally,utilizing specific-locus amplified fragment sequencing(SLAF-seq)technology,chromosome 5E-specific molecular markers were developed and validated,achieving a 33.3% success rate,facilitating marker-assisted selection for disease resistance in wheat.Overall,the CH97 substitution line,with its resistance to diseases and improved agronomic traits,represents valuable new germplasm for wheat chromosome engineering and breeding.展开更多
背景:许多研究将静电纺丝应用于人工硬膜替代物的研究中,设计了具有多功能的人工硬膜,不仅可以更好地模拟天然硬膜结构,还能发挥促愈合、防粘连、抗菌等生物学功能。目的:系统总结静电纺丝技术应用于人工硬膜替代物中的研究进展。方法:...背景:许多研究将静电纺丝应用于人工硬膜替代物的研究中,设计了具有多功能的人工硬膜,不仅可以更好地模拟天然硬膜结构,还能发挥促愈合、防粘连、抗菌等生物学功能。目的:系统总结静电纺丝技术应用于人工硬膜替代物中的研究进展。方法:以“静电纺丝硬膜,硬膜替代物,人工硬膜补片”为中文检索词,以“electrospinning dura mater,dura mater substitute,artificial dura mater patch”为英文检索词,分别检索中国知网、万方数据库和PubMed数据库,文献检索时限为各数据库建库至2024年9月,通过阅读题目和摘要进行初步筛选,排除与文章相关性较低的文献,最终纳入64篇文献进行归纳总结。结果与结论:人工硬膜主要分为不可降解和可降解两大类,在修复硬膜损伤中发挥重要作用,也是目前生物组织工程研究的热点。静电纺丝技术可通过结合化学、材料学、药学等学科从结构和功能需求上进行人工硬膜替代物的设计,制备出仿生取向纤维型、不对称双层型、多层复合型等一系列新型人工硬膜,同时还赋予人工硬膜密封、防粘连、抗菌和促进神经组织修复等多种重要的生物功能,使得人工硬膜由过去传统的单纯补片转变为多功能的组织生物支架。静电纺丝为以后开发和研究更加理想的人工硬膜替代物提供了帮助,也为解决硬膜损伤修复问题带来新的思路。展开更多
Benzoic acid containing fluorine atom at ortho-,meta-,and para-position are employed as self-assembled monolayers to modify the buried interface in perovskite solar cells(PSCs).It is demonstrated that the position of ...Benzoic acid containing fluorine atom at ortho-,meta-,and para-position are employed as self-assembled monolayers to modify the buried interface in perovskite solar cells(PSCs).It is demonstrated that the position of fluorine atom influences the passivation effect and para-fluorinated one provided the most substantial performance enhancement mainly originating from ameliorated contact and energy band alignment between NiOx and perovskite,improved perovskite quality and defect healing.Resultantly,PSC with a power conversion efficiency of 24%can be achieved.Meanwhile,which can maintain 96.8%of the initial PCE after a 1000 h storage,presenting enhanced durability.This work highlights the critical role of molecular functionality and conformation in the buried interface modification of PSCs,providing valuable insights for future developments.展开更多
基金funded by the Key R&D Program of Yangling Seed Industry Innovation,China(Ylzy-xm-02)the Young Elite Scientists Sponsorship Program by China Association for Science and Technology(2021QNRC001)。
文摘Thinopyrum ponticum(2n=10×=70),a wild relative of common wheat(Triticum aestivum L.),is considered an invaluable genetic resource for wheat improvement due to its abundance of genes conferring resistance to biotic and abiotic stresses.This study focused on the CH97 line,derived from the BC1F7 progeny of a cross between wheat cv.7182 and Th.ponticum.Cytological evidence showed that CH97 has 42 chromosomes,forming 21 bivalents at meiotic metaphase I,with the bivalents subsequently separating and moving to opposite poles during meiotic anaphase I.Through a combination of fluorescence in situ hybridization(FISH),genomic in situ hybridization(GISH),multicolor GISH(mc-GISH),and liquid array analysis,it was determined that CH97 comprises 40 wheat chromosomes and two alien chromosomes from the Ee genome of Th.ponticum,featuring the absence of a pair of 5D chromosomes and variations in 1B,6B,and 7B chromosomes.These findings confirm that CH97 is a stable wheat-Th.ponticum 5E(5D)alien disomic substitution line.Inoculation experiments revealed that CH97 exhibits high resistance to wheat powdery mildew and stripe rust throughout the growth period,in contrast to the highly susceptible common wheat parent 7182.Compared to 7182,CH97 displayed improvements in thousand-kernel weight and kernel length.Additionally,utilizing specific-locus amplified fragment sequencing(SLAF-seq)technology,chromosome 5E-specific molecular markers were developed and validated,achieving a 33.3% success rate,facilitating marker-assisted selection for disease resistance in wheat.Overall,the CH97 substitution line,with its resistance to diseases and improved agronomic traits,represents valuable new germplasm for wheat chromosome engineering and breeding.
文摘背景:许多研究将静电纺丝应用于人工硬膜替代物的研究中,设计了具有多功能的人工硬膜,不仅可以更好地模拟天然硬膜结构,还能发挥促愈合、防粘连、抗菌等生物学功能。目的:系统总结静电纺丝技术应用于人工硬膜替代物中的研究进展。方法:以“静电纺丝硬膜,硬膜替代物,人工硬膜补片”为中文检索词,以“electrospinning dura mater,dura mater substitute,artificial dura mater patch”为英文检索词,分别检索中国知网、万方数据库和PubMed数据库,文献检索时限为各数据库建库至2024年9月,通过阅读题目和摘要进行初步筛选,排除与文章相关性较低的文献,最终纳入64篇文献进行归纳总结。结果与结论:人工硬膜主要分为不可降解和可降解两大类,在修复硬膜损伤中发挥重要作用,也是目前生物组织工程研究的热点。静电纺丝技术可通过结合化学、材料学、药学等学科从结构和功能需求上进行人工硬膜替代物的设计,制备出仿生取向纤维型、不对称双层型、多层复合型等一系列新型人工硬膜,同时还赋予人工硬膜密封、防粘连、抗菌和促进神经组织修复等多种重要的生物功能,使得人工硬膜由过去传统的单纯补片转变为多功能的组织生物支架。静电纺丝为以后开发和研究更加理想的人工硬膜替代物提供了帮助,也为解决硬膜损伤修复问题带来新的思路。
基金the Key project of Nature Science Foundation of Tianjin(22JCZDJC00120)the 111 Project(B16027)for financial support.
文摘Benzoic acid containing fluorine atom at ortho-,meta-,and para-position are employed as self-assembled monolayers to modify the buried interface in perovskite solar cells(PSCs).It is demonstrated that the position of fluorine atom influences the passivation effect and para-fluorinated one provided the most substantial performance enhancement mainly originating from ameliorated contact and energy band alignment between NiOx and perovskite,improved perovskite quality and defect healing.Resultantly,PSC with a power conversion efficiency of 24%can be achieved.Meanwhile,which can maintain 96.8%of the initial PCE after a 1000 h storage,presenting enhanced durability.This work highlights the critical role of molecular functionality and conformation in the buried interface modification of PSCs,providing valuable insights for future developments.
