Objective To investigate the associations between eight serum per-and polyfluoroalkyl substances(PFASs)and regional fat depots,we analyzed the data from the National Health and Nutrition Examination Survey(NHANES)2011...Objective To investigate the associations between eight serum per-and polyfluoroalkyl substances(PFASs)and regional fat depots,we analyzed the data from the National Health and Nutrition Examination Survey(NHANES)2011-2018 cycles.Methods Multiple linear regression models were developed to explore the associations between serum PFAS concentrations and six fat compositions along with a fat distribution score created by summing the concentrations of the six fat compositions.The associations between structurally grouped PFASs and fat distribution were assessed,and a prediction model was developed to estimate the ability of PFAS exposure to predict obesity risk.Results Among females aged 39-59 years,trunk fat mass was positively associated with perfluorooctane sulfonate(PFOS).Higher concentrations of PFOS,perfluorohexane sulfonate(PFHxS),perfluorodecanoate(PFDeA),perfluorononanoate(PFNA),and n-perfluorooctanoate(n-PFOA)were linked to greater visceral adipose tissue in this group.In men,exposure to total perfluoroalkane sulfonates(PFSAs)and long-chain PFSAs was associated with reductions in abdominal fat,while higher abdominal fat in women aged 39-59 years was associated with short-chain PFSAs.The prediction model demonstrated high accuracy,with an area under the curve(AUC)of 0.9925 for predicting obesity risk.Conclusion PFAS exposure is associated with regional fat distribution,with varying effects based on age,sex,and PFAS structure.The findings highlight the potential role of PFAS exposure in influencing fat depots and obesity risk,with significant implications for public health.The prediction model provides a highly accurate tool for assessing obesity risk related to PFAS exposure.展开更多
Objectives:Psychological resilience is a critical resource for vocational high school students navigating social biases and fostering mental well-being.This six-month longitudinal study investigated the developmental ...Objectives:Psychological resilience is a critical resource for vocational high school students navigating social biases and fostering mental well-being.This six-month longitudinal study investigated the developmental trajectories of discrimination perception,vocational identity,and psychological resilience in this population.It further examined the longitudinal mediating role of vocational identity in the relationship between discrimination perception and psychological resilience.Methods:A total of 526 students from five vocational high schools in Guangdong,China,were assessed via convenience sampling at two time points:baseline(T1,September 2023)and six-month follow-up(T2,March 2024).Measures of discrimination perception,psychological resilience,and vocational identity were administered.Data were analyzed using a cross-lagged panel model to test for bidirectional relationships.Results:Over the six-month period,students showed significant decreases in discrimination perception and vocational identity,but a significant increase in psychological resilience.The cross-lagged model revealed significant bidirectional relationships:discrimination perception and psychological resilience negatively predicted each other over time(β=−0.124,p<0.01;β=−0.200,p<0.001),while psychological resilience and vocational identity positively predicted each other(β=0.084,p<0.05;β=0.076,p<0.05).The mediation analysis revealed a dual-pathway mechanism.T1 discrimination perception exerted both a significant direct negative effect on T2 psychological resilience(β=−0.332,p<0.001)and a significant indirect positive effect via T1 vocational identity(indirect effect=0.020,95%CI[0.001,0.046]).This confirms a partial mediating role,indicating that vocational identity functions as a compensatory mechanism,transforming the experience of discrimination perception into a potential source of psychological resilience.Conclusions:For vocational high school students,perception of discrimination directly undermines psychological resilience,but also indirectly fosters it through the positive development of vocational identity.These findings highlight vocational identity as a pivotal mechanism in the complex relationship between social adversity and mental resilience.展开更多
The purpose of this study is to gain perspectives on introducing IBCTL(International Baccalaureate Certificate in Teaching and Learning)into graduate school of teacher education in Japan,which are currently providing ...The purpose of this study is to gain perspectives on introducing IBCTL(International Baccalaureate Certificate in Teaching and Learning)into graduate school of teacher education in Japan,which are currently providing advanced teacher training programs aimed at cultivating student agency,a comprehensive set of skills necessary for children living in the future,as advocated by the Organisation for Economic Co-operation and Development(OECD)Education 2030 Project.To achieve this goal,I examined the curriculum and structure that are important for IB teacher training and the introduction of IBCTL.I attempted to achieve this goal by examining the curriculum being developed at a graduate school of teacher education attempting to implement IBCTL and the creation of a syllabus based on that curriculum through lesson planning.As a result,I examined the curriculum and structure as a perspective for introducing IBCTL into graduate school of teacher education in Japan,where advanced teacher training is required.I found that a core curriculum and structure that incorporates the core of the IBCTL into the core of graduate school of teacher education programs is important for implementing an IB education that is aligned with the advancement of teacher training and the development of student agency.展开更多
Adolescent smoking constitutes a critical public health challenge as early initiation increases the risk of premature mortality and smoking-related chronic diseases due to longer exposure and higher cumulative tobacco...Adolescent smoking constitutes a critical public health challenge as early initiation increases the risk of premature mortality and smoking-related chronic diseases due to longer exposure and higher cumulative tobacco use^([1]).Adolescents are especially prone to developing persistent smoking habits,with many adult smokers having started before the age of 18.In China,16.7%of secondary school students have tried smoking and 4.7%are current smokers,highlighting the critical need for targeted tobacco control interventions among the youth.展开更多
Inside the exhibition hall of the 2025 Osaka World Expo in Japan,crowds gathered before a cloisonne enamel painting titled The Pomegranate of Ethnic Unity.The pomegranate,outlined with gold filigree,appears full and r...Inside the exhibition hall of the 2025 Osaka World Expo in Japan,crowds gathered before a cloisonne enamel painting titled The Pomegranate of Ethnic Unity.The pomegranate,outlined with gold filigree,appears full and ripe;Tibetan auspicious motifs intertwine with Han-style cloud patterns,and the enamel glaze reflects brilliant colors under the lights.Created by teachers and students from Nyingchi Vocational and Technical School(hereafter“Nyingchi Vocational School”),this artwork not only drew admiration from countless visitors but also became a“silent envoy”of dialogue between Tibetan culture and the world.展开更多
Based upon the crystal structure of a previously reported fragment hit that binds to Corresponding author. β-secretase, a novel series of non-peptidic small-molecule β-secretase inhibitors, namely hexahydropyrimidin...Based upon the crystal structure of a previously reported fragment hit that binds to Corresponding author. β-secretase, a novel series of non-peptidic small-molecule β-secretase inhibitors, namely hexahydropyrimidin-5-ols, along with two series of their analogues, were rationally designed through structural modification. The CADD study was performed and revealed good expectation. Inhibitory activities of the corresponding structural cores were tested, which provided further support for our design approach.展开更多
Mycobacterium tuberculosis FabH, an essential enzyme in mycolic acids biosynthetic pathway, is an attractive target for novel anti-tuberculosis agents. Structure-based design, synthesis of novel inhibitors of mtFabH w...Mycobacterium tuberculosis FabH, an essential enzyme in mycolic acids biosynthetic pathway, is an attractive target for novel anti-tuberculosis agents. Structure-based design, synthesis of novel inhibitors of mtFabH was reported in this paper. A novel scaffold structure was designed, and 12 candidate compounds that displayed favorable binding with the active site were identified and synthesized. 2009 Song Li. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.展开更多
The hybrid atomistic structure-based model has been validated to be effective in investigation of G-quadruplex folding.In this study,we performed large-scale conventional all-atom simulations to complement the folding...The hybrid atomistic structure-based model has been validated to be effective in investigation of G-quadruplex folding.In this study,we performed large-scale conventional all-atom simulations to complement the folding mechanism of human telomeric sequence Htel24 revealed by a multi-basin hybrid atomistic structure-based model.Firstly,the real time-scale of folding rate,which cannot be obtained from the structure-based simulations,was estimated directly by constructing a Markov state model.The results show that Htel24 may fold as fast as on the order of milliseconds when only considering the competition between the hybrid-1 and hybrid-2 G-quadruplex conformations.Secondly,in comparison with the results of structure-based simulations,more metastable states were identified to participate in the formation of hybrid-1 and hybrid-2 conformations.These findings suggest that coupling the hybrid atomistic structure-based model and the conventional all-atom model can provide more insights into the folding dynamics of DNA G-quadruplex.As a result,the multiscale computational framework adopted in this study may be useful to study complex processes of biomolecules involving large conformational changes.展开更多
The mammalian target of rapamycin(mTOR) is a critical component of the PI3K-AKT signaling pathway. It is highly activated in cervical cancer, which continues to pose an important clinical challenge with an urgent need...The mammalian target of rapamycin(mTOR) is a critical component of the PI3K-AKT signaling pathway. It is highly activated in cervical cancer, which continues to pose an important clinical challenge with an urgent need for new and improved therapeutic approaches. Herein, we describe the structure-based drug discovery and biological evaluation of a series of m TOR kinase inhibitors as potential anti-cervical cancer agents. The results of enzymatic activity assays supported C3 as a potential m TOR inhibitor, which exhibited high inhibitory activity with an IC50 of 1.57 μM. Molecular docking and dynamics simulation were conducted to predict the binding patterns, suggesting relationships between structure and activity. The anti-proliferative assay against diverse cancer cell lines was displayed subsequently, revealing that C3 exhibited significant proliferation inhibition against cervical cancer cell He La(IC50=0.38μM) compared with other cell lines. Moreover, C3 could effectively reduce the expression of phospho-ribosomal S6 protein(p-S6) in He La cells in a dose-dependent manner. Noteworthily, m TOR signaling and other cellular pathways might contribute to the significant effect of C3 against cervical cancer simultaneously. These data indicated that C3 represented a good lead molecule for further development as a therapeutic agent for cervical cancer treatment.展开更多
Public English carries significant moral education responsibilities as a core general education course in vocational schools.By organically integrating English teaching content with moral education,it achieves the uni...Public English carries significant moral education responsibilities as a core general education course in vocational schools.By organically integrating English teaching content with moral education,it achieves the unity of knowledge imparting and value guidance.The course should fully leverage its educational function by strengthening humanistic care and psychological counseling,guiding students to develop self-awareness,understand their responsibilities,and establish correct worldviews,outlooks on life,and values,ultimately fostering well-rounded development.展开更多
Hello,everyone.My English name is Molly.I am a girl.I am 7 years old.I'm a primary school student.I'm in Class 4,Grade 2 at Beijing Jianhua ExperimentalSchooll.
Tom is an American student.He is ten years old.But he does not live in the USA now.He lives with his father,mother and grandfather in a small city in China.Tom has a Chinese name,Tang Mu.He is in Grade Three at a prim...Tom is an American student.He is ten years old.But he does not live in the USA now.He lives with his father,mother and grandfather in a small city in China.Tom has a Chinese name,Tang Mu.He is in Grade Three at a primary school.He goes to school from Monday to Friday.Every morning,he gets up at 6:30.He brushes his teeth and washes his face.Then he has breakfast with his family.His parents often cook eggs and noodles.At 7:30,Tom goes to school.He walks to school with his best friend,Mike.Mike is a Chinese boy.His Chinese name is Ma Ke.Mike is his English name.Tom's house is to the left of Mike's.It is about 80 metres away from Mike's.展开更多
Parental educational anxiety has become a social symptom in China,and rural primary school students’mothers exhibit unique educational anxieties due to their special living environment.Based on interviews with 10 rur...Parental educational anxiety has become a social symptom in China,and rural primary school students’mothers exhibit unique educational anxieties due to their special living environment.Based on interviews with 10 rural primary school students’mothers,five typical educational anxiety experiences were selected for analysis,and themes such as rural life burden,children’s learning habits,mothers’educational expectations,mothers’educational methods,mothers’emotional state,deviation between reality and expectations,homework guidance ability,mothers’educational level,and attitudes towards children’s future development were refined.The root causes of educational anxiety among rural primary school students’mothers include the deviation between children’s actual performance and mothers’educational expectations,the sense of disparity under social comparison,physical and mental exhaustion caused by role overload,anxiety triggered by excessive economic burden,and a sense of powerlessness towards children’s educational outcomes.To alleviate the educational anxiety of rural primary school students’mothers,mothers should actively adjust themselves,fathers should actively participate in their children’s education,society should create a healthy atmosphere,and schools should strengthen family education guidance.展开更多
In the process of daily psychological counseling,it is found that more and more school-age children have a dislike for learning,and the rate is increasing year by year.They are unclear about the purpose of learning,lo...In the process of daily psychological counseling,it is found that more and more school-age children have a dislike for learning,and the rate is increasing year by year.They are unclear about the purpose of learning,lose interest in class,do not listen carefully,cannot complete homework independently,and even skip school.This case analyzes the mechanism of a 7-year-old primary school student's aversion to learning and analyzes and discusses this behavior.展开更多
Background: Clinical decision support tools provide suggestions to support healthcare providers and clinicians, as they attend to patients. Clinicians use these tools to rapidly consult the evidence at the point of ca...Background: Clinical decision support tools provide suggestions to support healthcare providers and clinicians, as they attend to patients. Clinicians use these tools to rapidly consult the evidence at the point of care, a practice which has been found to reduce the time patients spend in hospitals, promote the quality of care and improve healthcare outcomes. Such tools include Medscape, VisualDx, Clinical Key, DynaMed, BMJ Best Practice and UpToDate. However, use of such tools has not yet been fully embraced in low-resource settings such as Uganda. Objective: This paper intends to collate data on the use and uptake of one such tool, UpToDate, which was provided at no cost to five medical schools in Uganda. Methods: Free access to UpToDate was granted through the IP addresses of five medical schools in Uganda in collaboration with Better Evidence at The Global Health Delivery Project at Harvard and Brigham and Women’s Hospital and Wolters Kluwer Health. Following the donation, medical librarians in the respective institutions conducted training sessions and created awareness of the tool. Usage data was aggregated, based on logins and content views, presented and analyzed using Excel tables and graphs. Results: The data shows similar trends in increased usage over the period of August 2022 to August 2023 across the five medical schools. The most common topics viewed, mode of access (using either the computer or the mobile app), total usage by institution, ratio of uses to eligible users by institution and ratio of uses to students by institution are shared. Conclusion: The study revealed that the tool was used by various user categories across the institutions with similar steady improved usage over the year. These results can inform the librarians as they encourage their respective institutions to continue using the tool to support uptake of point-of-care tools in clinical practice.展开更多
From the Lhasa River valley to the pastures of the northern plateau,the lights of boarding schools now stretch like a quiet,glowing ribbon across the high-altitude night sky.Sixty years ago,this ribbon did not exist;t...From the Lhasa River valley to the pastures of the northern plateau,the lights of boarding schools now stretch like a quiet,glowing ribbon across the high-altitude night sky.Sixty years ago,this ribbon did not exist;today,it has become one of the most heartwarming footnotes to Xizang's modernization.As the Xizang Autonomous Region marks its 60th founding anniversary,we retrace the development of boarding education and witness how the sense of community for the Chinese nation has taken root-in dormitories and classrooms alike-while also observing the transformation from"access to schooling"to"access to quality education"under the framework of regional ethnic autonomy.展开更多
Hello,everyone.My name is Guo Yunhan,and I am twelve years old.I study at Xinshidai Primary Schoolof Xinxiang County.Now I am in Class Fourr,Grade Six.I love my school very much.There are tall buildings and a big play...Hello,everyone.My name is Guo Yunhan,and I am twelve years old.I study at Xinshidai Primary Schoolof Xinxiang County.Now I am in Class Fourr,Grade Six.I love my school very much.There are tall buildings and a big playground in my school.The playground is wide and bright.We often do sports there,such as ping-pong,basketball,football,and badminton.Sometimes we run races,and sometimes we play games together.It is always full of laughter.展开更多
Objective:This study aimed to evaluate the effectiveness of an integrative educational intervention-combining hospital-based schooling programs,child-centered communication,and therapeutic play-on clinical,psychosocia...Objective:This study aimed to evaluate the effectiveness of an integrative educational intervention-combining hospital-based schooling programs,child-centered communication,and therapeutic play-on clinical,psychosocial,and educational outcomes among school-aged children with cancer.Materials and Methods:A quasi-experimental pre-and post-test control group design was conducted at the pediatric oncology unit of Rumah Sakit Islam Jemursari,Surabaya,Indonesia.Fifty-two hospitalized children aged 6-18 years were randomly assigned to an intervention group(n=26)or a control group(n=26).The intervention lasted 1 month and integrated hospital-based lessons aligned with the national curriculum,therapeutic play,and developmentally appropriate communication by trained staff.Outcomes included pain intensity(Wong-Baker FACES Scale),treatment adherence,quality of life(Pediatric Cancer Quality of Life-32[PCQL-32]),anxiety(Revised Children’s Manifest Anxiety Scale),and literacy-numeracy performance.Data were analyzed using paired t-tests,ANCOVA,and Cohen’s d,with significance set at P<0.05.Results:Compared with controls,the intervention group showed significant improvements in pain reduction(P<0.001),treatment adherence(P<0.001),and quality of life across all PCQL-32 domains(P<0.001).Anxiety levels decreased significantly(P<0.001),and academic performance in literacy and numeracy improved markedly(P<0.001).Conclusion:The integrative educational intervention effectively enhanced clinical recovery,psychosocial well-being,and academic continuity among hospitalized children with cancer,emphasizing the need to integrate hospital-based education and play-based communication into pediatric oncology care.展开更多
基金supported by the National Key R&D Program of China(No.2021YFC2700605)the National Natural Science Foundation of China(82071700 and 82101679)+4 种基金333 High-level Talent Training Project of the Jiangsu Province((2022)3-16-425)the Natural Science Foundation of Jiangsu Province(BK20220317)Jiangsu Funding Program for Excellent Postdoctoral Talent(2022ZB414)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(22KJB330001)China Postdoctoral Science Foundation(2022M711673).
文摘Objective To investigate the associations between eight serum per-and polyfluoroalkyl substances(PFASs)and regional fat depots,we analyzed the data from the National Health and Nutrition Examination Survey(NHANES)2011-2018 cycles.Methods Multiple linear regression models were developed to explore the associations between serum PFAS concentrations and six fat compositions along with a fat distribution score created by summing the concentrations of the six fat compositions.The associations between structurally grouped PFASs and fat distribution were assessed,and a prediction model was developed to estimate the ability of PFAS exposure to predict obesity risk.Results Among females aged 39-59 years,trunk fat mass was positively associated with perfluorooctane sulfonate(PFOS).Higher concentrations of PFOS,perfluorohexane sulfonate(PFHxS),perfluorodecanoate(PFDeA),perfluorononanoate(PFNA),and n-perfluorooctanoate(n-PFOA)were linked to greater visceral adipose tissue in this group.In men,exposure to total perfluoroalkane sulfonates(PFSAs)and long-chain PFSAs was associated with reductions in abdominal fat,while higher abdominal fat in women aged 39-59 years was associated with short-chain PFSAs.The prediction model demonstrated high accuracy,with an area under the curve(AUC)of 0.9925 for predicting obesity risk.Conclusion PFAS exposure is associated with regional fat distribution,with varying effects based on age,sex,and PFAS structure.The findings highlight the potential role of PFAS exposure in influencing fat depots and obesity risk,with significant implications for public health.The prediction model provides a highly accurate tool for assessing obesity risk related to PFAS exposure.
基金supported by the Guangdong Provincial Philosophy and Social Science“14th Five-Year Plan”Discipline Co-Construction Project(Grant No.GD22XJY14)the 2022 Guangdong Provincial Higher Education Teaching Reform Project(Grant No.Yue Jiao Gao[2023]4)Guangdong Polytechnic Normal University’s Project for Enhancing the Research Capacity of Doctoral Application Institution(Grant No.22GPNUZDJS48).
文摘Objectives:Psychological resilience is a critical resource for vocational high school students navigating social biases and fostering mental well-being.This six-month longitudinal study investigated the developmental trajectories of discrimination perception,vocational identity,and psychological resilience in this population.It further examined the longitudinal mediating role of vocational identity in the relationship between discrimination perception and psychological resilience.Methods:A total of 526 students from five vocational high schools in Guangdong,China,were assessed via convenience sampling at two time points:baseline(T1,September 2023)and six-month follow-up(T2,March 2024).Measures of discrimination perception,psychological resilience,and vocational identity were administered.Data were analyzed using a cross-lagged panel model to test for bidirectional relationships.Results:Over the six-month period,students showed significant decreases in discrimination perception and vocational identity,but a significant increase in psychological resilience.The cross-lagged model revealed significant bidirectional relationships:discrimination perception and psychological resilience negatively predicted each other over time(β=−0.124,p<0.01;β=−0.200,p<0.001),while psychological resilience and vocational identity positively predicted each other(β=0.084,p<0.05;β=0.076,p<0.05).The mediation analysis revealed a dual-pathway mechanism.T1 discrimination perception exerted both a significant direct negative effect on T2 psychological resilience(β=−0.332,p<0.001)and a significant indirect positive effect via T1 vocational identity(indirect effect=0.020,95%CI[0.001,0.046]).This confirms a partial mediating role,indicating that vocational identity functions as a compensatory mechanism,transforming the experience of discrimination perception into a potential source of psychological resilience.Conclusions:For vocational high school students,perception of discrimination directly undermines psychological resilience,but also indirectly fosters it through the positive development of vocational identity.These findings highlight vocational identity as a pivotal mechanism in the complex relationship between social adversity and mental resilience.
文摘The purpose of this study is to gain perspectives on introducing IBCTL(International Baccalaureate Certificate in Teaching and Learning)into graduate school of teacher education in Japan,which are currently providing advanced teacher training programs aimed at cultivating student agency,a comprehensive set of skills necessary for children living in the future,as advocated by the Organisation for Economic Co-operation and Development(OECD)Education 2030 Project.To achieve this goal,I examined the curriculum and structure that are important for IB teacher training and the introduction of IBCTL.I attempted to achieve this goal by examining the curriculum being developed at a graduate school of teacher education attempting to implement IBCTL and the creation of a syllabus based on that curriculum through lesson planning.As a result,I examined the curriculum and structure as a perspective for introducing IBCTL into graduate school of teacher education in Japan,where advanced teacher training is required.I found that a core curriculum and structure that incorporates the core of the IBCTL into the core of graduate school of teacher education programs is important for implementing an IB education that is aligned with the advancement of teacher training and the development of student agency.
基金supported by the World Health Organization Global Youth Tobacco Survey(WPDHP1206671)the Global Health Capacity Building and Tobacco Control Project:Tobacco Epidemic Monitoring and Comprehensive Tobacco Control Intervention。
文摘Adolescent smoking constitutes a critical public health challenge as early initiation increases the risk of premature mortality and smoking-related chronic diseases due to longer exposure and higher cumulative tobacco use^([1]).Adolescents are especially prone to developing persistent smoking habits,with many adult smokers having started before the age of 18.In China,16.7%of secondary school students have tried smoking and 4.7%are current smokers,highlighting the critical need for targeted tobacco control interventions among the youth.
文摘Inside the exhibition hall of the 2025 Osaka World Expo in Japan,crowds gathered before a cloisonne enamel painting titled The Pomegranate of Ethnic Unity.The pomegranate,outlined with gold filigree,appears full and ripe;Tibetan auspicious motifs intertwine with Han-style cloud patterns,and the enamel glaze reflects brilliant colors under the lights.Created by teachers and students from Nyingchi Vocational and Technical School(hereafter“Nyingchi Vocational School”),this artwork not only drew admiration from countless visitors but also became a“silent envoy”of dialogue between Tibetan culture and the world.
基金National Natural Science Foundation of China (Grant No.20772008 and 30772650)
文摘Based upon the crystal structure of a previously reported fragment hit that binds to Corresponding author. β-secretase, a novel series of non-peptidic small-molecule β-secretase inhibitors, namely hexahydropyrimidin-5-ols, along with two series of their analogues, were rationally designed through structural modification. The CADD study was performed and revealed good expectation. Inhibitory activities of the corresponding structural cores were tested, which provided further support for our design approach.
基金supported by the National Basic Research Program of China(No.2004CB518908)the National High Technology Research and Development Program of China(No.2006AA020601)
文摘Mycobacterium tuberculosis FabH, an essential enzyme in mycolic acids biosynthetic pathway, is an attractive target for novel anti-tuberculosis agents. Structure-based design, synthesis of novel inhibitors of mtFabH was reported in this paper. A novel scaffold structure was designed, and 12 candidate compounds that displayed favorable binding with the active site were identified and synthesized. 2009 Song Li. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
基金the National Natural Science Foundation of China(Grant Nos.11504043,61671107,31670727,and 61771093)the Science Foundation of Shandong Province of China(Grant No.ZR2016JL027)+1 种基金the Taishan Young Scholars Program of Shandong Province of China(Grant No.tsqn20161049)the Youth Science and Technology Innovation Plan of Universities in Shandong,China(Grant No.2019KJE007)。
文摘The hybrid atomistic structure-based model has been validated to be effective in investigation of G-quadruplex folding.In this study,we performed large-scale conventional all-atom simulations to complement the folding mechanism of human telomeric sequence Htel24 revealed by a multi-basin hybrid atomistic structure-based model.Firstly,the real time-scale of folding rate,which cannot be obtained from the structure-based simulations,was estimated directly by constructing a Markov state model.The results show that Htel24 may fold as fast as on the order of milliseconds when only considering the competition between the hybrid-1 and hybrid-2 G-quadruplex conformations.Secondly,in comparison with the results of structure-based simulations,more metastable states were identified to participate in the formation of hybrid-1 and hybrid-2 conformations.These findings suggest that coupling the hybrid atomistic structure-based model and the conventional all-atom model can provide more insights into the folding dynamics of DNA G-quadruplex.As a result,the multiscale computational framework adopted in this study may be useful to study complex processes of biomolecules involving large conformational changes.
基金National Natural Science Foundation of China(Grant No.21772005,81872730)the Beijing Natural Science Foundation(Grant No.7202088,7172118)。
文摘The mammalian target of rapamycin(mTOR) is a critical component of the PI3K-AKT signaling pathway. It is highly activated in cervical cancer, which continues to pose an important clinical challenge with an urgent need for new and improved therapeutic approaches. Herein, we describe the structure-based drug discovery and biological evaluation of a series of m TOR kinase inhibitors as potential anti-cervical cancer agents. The results of enzymatic activity assays supported C3 as a potential m TOR inhibitor, which exhibited high inhibitory activity with an IC50 of 1.57 μM. Molecular docking and dynamics simulation were conducted to predict the binding patterns, suggesting relationships between structure and activity. The anti-proliferative assay against diverse cancer cell lines was displayed subsequently, revealing that C3 exhibited significant proliferation inhibition against cervical cancer cell He La(IC50=0.38μM) compared with other cell lines. Moreover, C3 could effectively reduce the expression of phospho-ribosomal S6 protein(p-S6) in He La cells in a dose-dependent manner. Noteworthily, m TOR signaling and other cellular pathways might contribute to the significant effect of C3 against cervical cancer simultaneously. These data indicated that C3 represented a good lead molecule for further development as a therapeutic agent for cervical cancer treatment.
基金2024 Jilin Province Vocational and Adult Education Teaching Reform Research Project,“Ideological and Political Education in Public English Curriculum of Vocational Schools:Construction and Practice”(Project No.:2024ZCY352)。
文摘Public English carries significant moral education responsibilities as a core general education course in vocational schools.By organically integrating English teaching content with moral education,it achieves the unity of knowledge imparting and value guidance.The course should fully leverage its educational function by strengthening humanistic care and psychological counseling,guiding students to develop self-awareness,understand their responsibilities,and establish correct worldviews,outlooks on life,and values,ultimately fostering well-rounded development.
文摘Hello,everyone.My English name is Molly.I am a girl.I am 7 years old.I'm a primary school student.I'm in Class 4,Grade 2 at Beijing Jianhua ExperimentalSchooll.
文摘Tom is an American student.He is ten years old.But he does not live in the USA now.He lives with his father,mother and grandfather in a small city in China.Tom has a Chinese name,Tang Mu.He is in Grade Three at a primary school.He goes to school from Monday to Friday.Every morning,he gets up at 6:30.He brushes his teeth and washes his face.Then he has breakfast with his family.His parents often cook eggs and noodles.At 7:30,Tom goes to school.He walks to school with his best friend,Mike.Mike is a Chinese boy.His Chinese name is Ma Ke.Mike is his English name.Tom's house is to the left of Mike's.It is about 80 metres away from Mike's.
基金Hunan Provincial Social Science Foundation“A Phenomenological Study on the Educational Life Experiences of Rural Young Teachers”(20YBA017)。
文摘Parental educational anxiety has become a social symptom in China,and rural primary school students’mothers exhibit unique educational anxieties due to their special living environment.Based on interviews with 10 rural primary school students’mothers,five typical educational anxiety experiences were selected for analysis,and themes such as rural life burden,children’s learning habits,mothers’educational expectations,mothers’educational methods,mothers’emotional state,deviation between reality and expectations,homework guidance ability,mothers’educational level,and attitudes towards children’s future development were refined.The root causes of educational anxiety among rural primary school students’mothers include the deviation between children’s actual performance and mothers’educational expectations,the sense of disparity under social comparison,physical and mental exhaustion caused by role overload,anxiety triggered by excessive economic burden,and a sense of powerlessness towards children’s educational outcomes.To alleviate the educational anxiety of rural primary school students’mothers,mothers should actively adjust themselves,fathers should actively participate in their children’s education,society should create a healthy atmosphere,and schools should strengthen family education guidance.
文摘In the process of daily psychological counseling,it is found that more and more school-age children have a dislike for learning,and the rate is increasing year by year.They are unclear about the purpose of learning,lose interest in class,do not listen carefully,cannot complete homework independently,and even skip school.This case analyzes the mechanism of a 7-year-old primary school student's aversion to learning and analyzes and discusses this behavior.
文摘Background: Clinical decision support tools provide suggestions to support healthcare providers and clinicians, as they attend to patients. Clinicians use these tools to rapidly consult the evidence at the point of care, a practice which has been found to reduce the time patients spend in hospitals, promote the quality of care and improve healthcare outcomes. Such tools include Medscape, VisualDx, Clinical Key, DynaMed, BMJ Best Practice and UpToDate. However, use of such tools has not yet been fully embraced in low-resource settings such as Uganda. Objective: This paper intends to collate data on the use and uptake of one such tool, UpToDate, which was provided at no cost to five medical schools in Uganda. Methods: Free access to UpToDate was granted through the IP addresses of five medical schools in Uganda in collaboration with Better Evidence at The Global Health Delivery Project at Harvard and Brigham and Women’s Hospital and Wolters Kluwer Health. Following the donation, medical librarians in the respective institutions conducted training sessions and created awareness of the tool. Usage data was aggregated, based on logins and content views, presented and analyzed using Excel tables and graphs. Results: The data shows similar trends in increased usage over the period of August 2022 to August 2023 across the five medical schools. The most common topics viewed, mode of access (using either the computer or the mobile app), total usage by institution, ratio of uses to eligible users by institution and ratio of uses to students by institution are shared. Conclusion: The study revealed that the tool was used by various user categories across the institutions with similar steady improved usage over the year. These results can inform the librarians as they encourage their respective institutions to continue using the tool to support uptake of point-of-care tools in clinical practice.
文摘From the Lhasa River valley to the pastures of the northern plateau,the lights of boarding schools now stretch like a quiet,glowing ribbon across the high-altitude night sky.Sixty years ago,this ribbon did not exist;today,it has become one of the most heartwarming footnotes to Xizang's modernization.As the Xizang Autonomous Region marks its 60th founding anniversary,we retrace the development of boarding education and witness how the sense of community for the Chinese nation has taken root-in dormitories and classrooms alike-while also observing the transformation from"access to schooling"to"access to quality education"under the framework of regional ethnic autonomy.
文摘Hello,everyone.My name is Guo Yunhan,and I am twelve years old.I study at Xinshidai Primary Schoolof Xinxiang County.Now I am in Class Fourr,Grade Six.I love my school very much.There are tall buildings and a big playground in my school.The playground is wide and bright.We often do sports there,such as ping-pong,basketball,football,and badminton.Sometimes we run races,and sometimes we play games together.It is always full of laughter.
文摘Objective:This study aimed to evaluate the effectiveness of an integrative educational intervention-combining hospital-based schooling programs,child-centered communication,and therapeutic play-on clinical,psychosocial,and educational outcomes among school-aged children with cancer.Materials and Methods:A quasi-experimental pre-and post-test control group design was conducted at the pediatric oncology unit of Rumah Sakit Islam Jemursari,Surabaya,Indonesia.Fifty-two hospitalized children aged 6-18 years were randomly assigned to an intervention group(n=26)or a control group(n=26).The intervention lasted 1 month and integrated hospital-based lessons aligned with the national curriculum,therapeutic play,and developmentally appropriate communication by trained staff.Outcomes included pain intensity(Wong-Baker FACES Scale),treatment adherence,quality of life(Pediatric Cancer Quality of Life-32[PCQL-32]),anxiety(Revised Children’s Manifest Anxiety Scale),and literacy-numeracy performance.Data were analyzed using paired t-tests,ANCOVA,and Cohen’s d,with significance set at P<0.05.Results:Compared with controls,the intervention group showed significant improvements in pain reduction(P<0.001),treatment adherence(P<0.001),and quality of life across all PCQL-32 domains(P<0.001).Anxiety levels decreased significantly(P<0.001),and academic performance in literacy and numeracy improved markedly(P<0.001).Conclusion:The integrative educational intervention effectively enhanced clinical recovery,psychosocial well-being,and academic continuity among hospitalized children with cancer,emphasizing the need to integrate hospital-based education and play-based communication into pediatric oncology care.
