Large cutaneous wounds pose a severe medical problem and may be deadly in cases when regeneration is impaired.Recently,topical stem cell therapy has been realized as a promising strategy for wound healing and skin reg...Large cutaneous wounds pose a severe medical problem and may be deadly in cases when regeneration is impaired.Recently,topical stem cell therapy has been realized as a promising strategy for wound healing and skin regeneration.However,stem cells must be administrated uniformly to the wound area,otherwise treatment will be ineffective,which has been a limitation of current administration methods.Specifically,the delivery pressure and nozzle features of most clinical cell spray devices are unknown,which may significantly affect the viability of sprayed cells and their capacity for proliferation.Herein,we developed a novel pneumatically assisted atomization device(PAAD)in which cell suspensions were uniformly atomized at a low delivery pressure.We optimized the applied fluidic pressure and air pressure to maximize cell survival and function for the application of multiple cell types,while ensuring uniform dispersal across the wound site.Moreover,we found that the application of sprayed umbilical cord-derived mesenchymal stem cells to wound sites significantly accelerated wound healing and promoted appendage regeneration on an excisional cutaneous wound model.Overall,the novel PAAD system delivered living cells uniformly and maintained the viability and differentiation of sprayed cells,strongly suggesting its potential for application in clinical cell therapy,especially for large,irregular,and severe skin wounds.展开更多
This study evaluated corn(Zea mays var.indentata)canopy vigor and temperature variations using small unmanned aerial system(UAS)based spatiotemporal imagery data.The key objective was to develop understanding on site-...This study evaluated corn(Zea mays var.indentata)canopy vigor and temperature variations using small unmanned aerial system(UAS)based spatiotemporal imagery data.The key objective was to develop understanding on site-specific suitability of the Mid Elevation Spray Application(MESA)and Low Elevation Spray Application(LESA)sprinkler systems in irrigating corn crop and potential water as well as energy savings.Aerial data was collected through small UAS flights at 100m height above ground level and on 49,59,65,77,105,114,134 days after planting(DAP).Small UAS had 5-band multispectral and radiometric thermal imager on-board the platform.Custom image processing algorithms were developed to extract various vegetation indices(Normalized difference vegetation index[NDVI],Green NDVI[GNDVI]and Normalized difference red edge[NDRE])and canopy temperature maps from the imagery data.Two sample T-test was performed on extracted data to understand significant difference,at 5%level,within the LESA and MESA treatments.For both the irrigation techniques,the crop vigor increased in the early growth stage(49,65 DAP),peaked in the mid growth stage(77,105,114 DAP)and then decreased in the late growth stage(134 DAP).The MESA irrigated sections had higher vigor(NDVI,GNDVI,NDRE),but not significant,compared to LESA throughout the season.Similarly,the MESA irrigated areas had significantly(0.61–2.07C)cooler canopies than LESA.Such results were anticipated,in part due to the issues with the sprinkler heads used in LESA.The heads were being pulled off in the corn field,causing the weighted hose to damage the crop.A different kind of sprinkler head was used after this incident.However,some strips of corn had already been damaged.Overall,this study results confirm suitability of aerial imagery data in evaluating pertinent irrigation treatments.Additional season’s data would be needed to clearly understand which technique(LESA or MESA)is more suitable for irrigating corn crop in the central part of state of Washington.展开更多
Nozzle flowrate and spray pressure are two of the most important factors influencing on droplet characteristics.With the aim to develop prediction models for air-induction nozzles(AINs),a series of Billericay Farm Ser...Nozzle flowrate and spray pressure are two of the most important factors influencing on droplet characteristics.With the aim to develop prediction models for air-induction nozzles(AINs),a series of Billericay Farm Services(BFS)AINs with different orifice diameters in combination with tap water were tested.0.2 MPa,0.3 MPa,0.4 MPa,0.5 MPa,0.6 MPa and 0.7 MPa of spray pressures and 2 m/s,3 m/s,4 m/s and 5 m/s of air speeds were setup.Based on the wind tunnel tests data,prediction models with input variables of nozzle flowrate and spray pressure and output variables of D_(v0.1),D_(v0.5),D_(v0.9),%<150μm(proportion of spray volume contained in droplets with diameter below 150μm),relative span(RS)and coefficient of variation(CV)of D_(v0.5) were developed.The developed models were validated based on wind tunnel experimental data.Results showed that:for D_(v0.1),D_(v0.5),D_(v0.9) and%<150μm,R^(2) were equal to 0.768,0.823,0.868 and 0.811,indicating that the predictive ability for these four parameters is strong.For RS and CV,R^(2) were equal to 0.100 and 0.113,respectively,indicating that the predictive ability for these two parameters is poor.The models developed in the present study are helpful for facilitating the use of AIN in agricultural spray application.展开更多
基金the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16020807)the Key Research and Development Program of Jiangsu Province,China(Nos.BE2018668 and BE2017669)+2 种基金the Major Innovative Research Team of Suzhou,China(No.ZXT2019007)SIBET and Jilin City Science and Technology Cooperation Project(No.E0550104)Doctor of Entrepreneurship and Innovation Program of Jiangsu Province in the year of 2020.
文摘Large cutaneous wounds pose a severe medical problem and may be deadly in cases when regeneration is impaired.Recently,topical stem cell therapy has been realized as a promising strategy for wound healing and skin regeneration.However,stem cells must be administrated uniformly to the wound area,otherwise treatment will be ineffective,which has been a limitation of current administration methods.Specifically,the delivery pressure and nozzle features of most clinical cell spray devices are unknown,which may significantly affect the viability of sprayed cells and their capacity for proliferation.Herein,we developed a novel pneumatically assisted atomization device(PAAD)in which cell suspensions were uniformly atomized at a low delivery pressure.We optimized the applied fluidic pressure and air pressure to maximize cell survival and function for the application of multiple cell types,while ensuring uniform dispersal across the wound site.Moreover,we found that the application of sprayed umbilical cord-derived mesenchymal stem cells to wound sites significantly accelerated wound healing and promoted appendage regeneration on an excisional cutaneous wound model.Overall,the novel PAAD system delivered living cells uniformly and maintained the viability and differentiation of sprayed cells,strongly suggesting its potential for application in clinical cell therapy,especially for large,irregular,and severe skin wounds.
基金This project was funded in part by State ofWashingtonWater Research Center and USDA National Institute for Food and Agriculture Projects#WNP00745 and WNP0839.Authors would like to acknowledge help of Rajeev Sinha,Haitham Bahlol and Azeem Khan in completion of this study.
文摘This study evaluated corn(Zea mays var.indentata)canopy vigor and temperature variations using small unmanned aerial system(UAS)based spatiotemporal imagery data.The key objective was to develop understanding on site-specific suitability of the Mid Elevation Spray Application(MESA)and Low Elevation Spray Application(LESA)sprinkler systems in irrigating corn crop and potential water as well as energy savings.Aerial data was collected through small UAS flights at 100m height above ground level and on 49,59,65,77,105,114,134 days after planting(DAP).Small UAS had 5-band multispectral and radiometric thermal imager on-board the platform.Custom image processing algorithms were developed to extract various vegetation indices(Normalized difference vegetation index[NDVI],Green NDVI[GNDVI]and Normalized difference red edge[NDRE])and canopy temperature maps from the imagery data.Two sample T-test was performed on extracted data to understand significant difference,at 5%level,within the LESA and MESA treatments.For both the irrigation techniques,the crop vigor increased in the early growth stage(49,65 DAP),peaked in the mid growth stage(77,105,114 DAP)and then decreased in the late growth stage(134 DAP).The MESA irrigated sections had higher vigor(NDVI,GNDVI,NDRE),but not significant,compared to LESA throughout the season.Similarly,the MESA irrigated areas had significantly(0.61–2.07C)cooler canopies than LESA.Such results were anticipated,in part due to the issues with the sprinkler heads used in LESA.The heads were being pulled off in the corn field,causing the weighted hose to damage the crop.A different kind of sprinkler head was used after this incident.However,some strips of corn had already been damaged.Overall,this study results confirm suitability of aerial imagery data in evaluating pertinent irrigation treatments.Additional season’s data would be needed to clearly understand which technique(LESA or MESA)is more suitable for irrigating corn crop in the central part of state of Washington.
基金This work was financially supported by the National Natural Science Foundation of China(31901401 and 31871520)the National Key R&D Program of China(2018YFD0200301)+3 种基金Science and Technology Plan of Guangdong Province of China(2017B090903007)Innovative Research Team of Guangdong Province Agriculture Research System(2017LM2153)Science and Technology Plan of Guangdong Province of China(2017B090907031)the Natural Science Foundation of Jiangsu Province(Project No.BK20160510).
文摘Nozzle flowrate and spray pressure are two of the most important factors influencing on droplet characteristics.With the aim to develop prediction models for air-induction nozzles(AINs),a series of Billericay Farm Services(BFS)AINs with different orifice diameters in combination with tap water were tested.0.2 MPa,0.3 MPa,0.4 MPa,0.5 MPa,0.6 MPa and 0.7 MPa of spray pressures and 2 m/s,3 m/s,4 m/s and 5 m/s of air speeds were setup.Based on the wind tunnel tests data,prediction models with input variables of nozzle flowrate and spray pressure and output variables of D_(v0.1),D_(v0.5),D_(v0.9),%<150μm(proportion of spray volume contained in droplets with diameter below 150μm),relative span(RS)and coefficient of variation(CV)of D_(v0.5) were developed.The developed models were validated based on wind tunnel experimental data.Results showed that:for D_(v0.1),D_(v0.5),D_(v0.9) and%<150μm,R^(2) were equal to 0.768,0.823,0.868 and 0.811,indicating that the predictive ability for these four parameters is strong.For RS and CV,R^(2) were equal to 0.100 and 0.113,respectively,indicating that the predictive ability for these two parameters is poor.The models developed in the present study are helpful for facilitating the use of AIN in agricultural spray application.
