We report a comprehensive numerical study of super resolution (SR) structured illumination microscopy (SIM) utilizing the classic Heintzmann-Cremer SIM process and algorithm. In particular, we investigated the impact ...We report a comprehensive numerical study of super resolution (SR) structured illumination microscopy (SIM) utilizing the classic Heintzmann-Cremer SIM process and algorithm. In particular, we investigated the impact of the diffraction limit of the underlying imaging system on the optimal SIM grating frequency that can be used to obtain the highest SR enhancement with non-continuous spatial frequency support. Besides confirming the previous theoretical and experimental work that SR-SIM can achieve an enhancement close to 3 times the diffraction limit with grating pattern illuminations, we also observe and report a series of more subtle effects of SR-SIM with non-continuous spatial frequency support. Our simulations show that when the SIM grating frequency exceeds twice that of the diffraction limit, the higher SIM grating frequency can help achieve a higher SR enhancement for the underlying imaging systems whose diffraction limit is low, though this enhancement is obtained at the cost of losing resolution at some lower resolution targets. Our simulations also show that, for underlying imaging systems with high diffraction limits, however, SR-SIM grating frequencies above twice the diffraction limits tend to bring no significant extra enhancement. Furthermore, we observed that there exists a limit grating frequency above which the SR enhancement effect is lost, and the reconstructed images essentially have the same resolution as the one obtained directly from the underlying imaging system without using the SIM process.展开更多
The purpose of this study is to examine optical spatial frequency spectroscopy analysis(SFSA)combined with visible resonance Raman(VRR)spectroscopic method,for thefirst time,to discriminate human brain metastases of l...The purpose of this study is to examine optical spatial frequency spectroscopy analysis(SFSA)combined with visible resonance Raman(VRR)spectroscopic method,for thefirst time,to discriminate human brain metastases of lung cancers adenocarcinoma(ADC)and squamous cell carcinoma(SCC)from normal tissues.A total of 31 label-free micrographic images of three type of brain tissues were obtained using a confocal micro-Raman spectroscopic system.VRR spectra of the corresponding samples were synchronously collected using excitation wavelength of 532 nm from the same sites of the tissues.Using SFSA method,the difference in the randomness of spatial frequency structures in the micrograph images was analyzed using Gaussian functionfitting.The standard deviations,calculated from the spatial frequencies of the micrograph images were then analyzed using support vector machine(SVM)classifier.The key VRR biomolecularfingerprints of carotenoids,tryptophan,amide II,lipids and proteins(methylene/methyl groups)were also analyzed using SVM classifier.All three types of brain tissues were identified with high accuracy in the two approaches with high correlation.The results show that SFSA–VRR can potentially be a dual-modal method to provide new criteria for identifying the three types of human brain tissues,which are on-site,real-time and label-free and may improve the accuracy of brain biopsy.展开更多
Imaging quality is a critical component of compressive imaging in real applications. In this study, we propose a compressive imaging method based on multi-scale modulation and reconstruction in the spatial frequency d...Imaging quality is a critical component of compressive imaging in real applications. In this study, we propose a compressive imaging method based on multi-scale modulation and reconstruction in the spatial frequency domain. Theoretical analysis and simulation show the relation between the measurement matrix resolution and compressive sensing(CS)imaging quality. The matrix design is improved to provide multi-scale modulations, followed by individual reconstruction of images of different spatial frequencies. Compared with traditional single-scale CS imaging, the multi-scale method provides high quality imaging in both high and low frequencies, and effectively decreases the overall reconstruction error.Experimental results confirm the feasibility of this technique, especially at low sampling rate. The method may thus be helpful in promoting the implementation of compressive imaging in real applications.展开更多
Electrical contact interfaces,integral to devices such as connectors,switches,and conductive slip rings,play a crucial role in determining current-carrying capabilities.This study adopts spatial frequency analysis to ...Electrical contact interfaces,integral to devices such as connectors,switches,and conductive slip rings,play a crucial role in determining current-carrying capabilities.This study adopts spatial frequency analysis to assess the currentcarrying performance of interfaces based on their morphological characteristics.Initially,rough surfaces with various parameters,including the cutoff frequency(N),the spectral index(β),and the gain factor(A),were generated using spatial frequency methods,followed by current-carrying simulations.The findings reveal that the characteristic parameters derived from spatial frequency methods exhibit a significantly stronger correlation with current-carrying performance than traditional roughness metrics.To enhance the practical applicability of this research,a physical-informed machine learning approach for rough surface features extraction was developed,demonstrating high predictive parameter identification accuracy for the three parameters.Overall,this paper introduces a tribo-informatics approach for analyzing and extracting rough surface features that considers the curvature characteristics of contact points.This approach holds significant potential for evaluating the processing state of current-carrying interfaces,selecting high-quality surfaces,and forecasting current-carrying performances.展开更多
Femtosecond laser-induced periodic surface structures(LIPSS)have been extensively studied over the past few decades.In particular,the period and groove width of high-spatial-frequency LIPSS(HSFL)is much smaller than t...Femtosecond laser-induced periodic surface structures(LIPSS)have been extensively studied over the past few decades.In particular,the period and groove width of high-spatial-frequency LIPSS(HSFL)is much smaller than the diffraction limit,making it a useful method for efficient nanomanufacturing.However,compared with the low-spatial-frequency LIPSS(LSFL),the structure size of the HSFL is smaller,and it is more easily submerged.Therefore,the formation mechanism of HSFL is complex and has always been a research hotspot in this field.In this study,regular LSFL with a period of 760 nm was fabricated in advance on a silicon surface with two-beam interference using an 800 nm,50 fs femtosecond laser.The ultrafast dynamics of HSFL formation on the silicon surface of prefabricated LSFL under single femtosecond laser pulse irradiation were observed and analyzed for the first time using collinear pump-probe imaging method.In general,the evolution of the surface structure undergoes five sequential stages:the LSFL begins to split,becomes uniform HSFL,degenerates into an irregular LSFL,undergoes secondary splitting into a weakly uniform HSFL,and evolves into an irregular LSFL or is submerged.The results indicate that the local enhancement of the submerged nanocavity,or the nanoplasma,in the prefabricated LSFL ridge led to the splitting of the LSFL,and the thermodynamic effect drove the homogenization of the splitting LSFL,which evolved into HSFL.展开更多
The large-aperture pulse compression grating(PCG) is a critical component in generating an ultra-high-intensity, ultra-short-pulse laser;however, the size of the PCG manufactured by transmission holographic exposure i...The large-aperture pulse compression grating(PCG) is a critical component in generating an ultra-high-intensity, ultra-short-pulse laser;however, the size of the PCG manufactured by transmission holographic exposure is limited to large-scale high-quality materials. The reflective method is a potential way for solving the size limitation, but there is still no successful precedent due to the lack of scientific specifications and advanced processing technology of exposure mirrors. In this paper, an analytical model is developed to clarify the specifications of components, and advanced processing technology is adopted to control the spatial frequency errors. Hereafter, we have successfully fabricated a multilayer dielectric grating of 200 mm × 150 mm by using an off-axis reflective exposure system with Φ300 mm. This demonstration proves that PCGs can be manufactured by using the reflection holographic exposure method and shows the potential for manufacturing the meter-level gratings used in 100 petawatt class high-power lasers.展开更多
Cultivated land quality(CLQ)is related to national food security.Rapid and high-precision monitoring of CLQ is crucial for the sustainable development of agriculture.However,current satellite image-based evaluation me...Cultivated land quality(CLQ)is related to national food security.Rapid and high-precision monitoring of CLQ is crucial for the sustainable development of agriculture.However,current satellite image-based evaluation methods that only consider the crop's spatial spectrum characteristics in the key growth stages cannot accurately estimate CLQ.This study proposes a new method based on time-series spectral data of crop growth to improve the accuracy of CLQ estimation.This study was conducted in the Conghua District of Guangzhou,Guangdong Province,China.The results showed that seven spectral indicators were determined as the optimal indicators based on the gradient boosting decision tree(GBDT)and variance inflation factor(VIF).And the genetic algorithm-back propagation neural network(GA-BPNN)model provided more accurate CLQ estimates than the partial least squares regression(PLSR)model,indicating a nonlinear relationship between CLQ and the indicators.In addition,the GA-BPNN model with a normalized root mean square error(NRMSE)of 9.91%demonstrates the excellent potential for mapping CLQ over large areas.The model based on the seven optimal indicators of crop phenology provided higher accuracy than the GA-BPNN model based on the normalized difference vegetation index(NDVI)indicators in the spatial domain,significantly decreasing the NRMSE of the CLQ estimates by 3.17%.This further implied that the spectral indicators in the spatial frequency domain can improve the accuracy of estimating CLQ.展开更多
A technique for coherent imaging based on spatial frequency heterodyning is described. Three images corresponding to three physical measurements are recorded. For the first measurement, a scene is simply illuminated w...A technique for coherent imaging based on spatial frequency heterodyning is described. Three images corresponding to three physical measurements are recorded. For the first measurement, a scene is simply illuminated with a coherent beam and for measurements 2 and 3, the scene is projected with cosine and sine fringes, respectively. Due to spatial frequency heterodyning, upper and lower side hand information falls in the pass band of the imager. These bands are separated and correct phases and positions are assigned to these bands in the spatial frequency domain. An extension of bandwidth is achieved in the frequency domain and the inverse frequency domain data then give a high resolution coherent image.展开更多
Spatial frequency shift(SFS) microscopy with evanescent wave illumination shows intriguing advantages, including large field of view(FOV), high speed, and good modularity. However, a missing band in the spatial freque...Spatial frequency shift(SFS) microscopy with evanescent wave illumination shows intriguing advantages, including large field of view(FOV), high speed, and good modularity. However, a missing band in the spatial frequency domain hampers the SFS superresolution microscopy from achieving resolution better than 3 folds of the Abbe diffraction limit. Here, we propose a novel tunable large-SFS microscopy, making the resolution improvement of a linear system no longer restricted by the detection numerical aperture(NA). The complete wide-range detection in the spatial frequency domain is realized by tuning the illumination spatial frequency actively and broadly through an angle modulation between the azimuthal propagating directions of two evanescent waves. The vertical spatial frequency is tuned via a sectional saturation effect, and the reconstructed depth information can be added to the lateral superresolution mask for 3D imaging. A lateral resolution of λ/9, and a vertical localization precision of ~λ/200(detection objective NA = 0.9) are realized with a gallium phosphide(GaP) waveguide. Its unlimited resolution enhancing capability is demonstrated by introducing a designed metamaterial chip with an unusual large refractive index. Besides the great resolution enhancement, this method shows better anti-noise capability than classical structured illumination microscopy without SFS tunability. This method is chip-compatible and can potentially provide a massproducible illumination chip module achieving the fast, large-FOV, and deep-subwavelength 3D nanoscopy.展开更多
A frequency and spatial domain decomposition method （FSDD） for operational modal analysis （OMA） is presented in this paper, which is an extension of the complex mode indicator function （CMIF） method for experime...A frequency and spatial domain decomposition method （FSDD） for operational modal analysis （OMA） is presented in this paper, which is an extension of the complex mode indicator function （CMIF） method for experimental modal analysis （EMA）. The theoretical background of the FSDD method is clarified, Singular value decomposition is adopted to separate the signal space from the noise space. Finally, an enhanced power spectrum density （PSD） is proposed to obtain more accurate modal parameters by curve fitting in the frequency domain. Moreover, a simulation case and an application case are used to validate this method.展开更多
Objective To examine whether the selectivity of visual cortical neurons to stimulus spatial frequencies would be affected by aging in cats.Methods In vivo extracellular single-unit recording techniques were employed t...Objective To examine whether the selectivity of visual cortical neurons to stimulus spatial frequencies would be affected by aging in cats.Methods In vivo extracellular single-unit recording techniques were employed to record the tuning responses of V1 neurons to different stimulus spatial frequencies in old and young adult cats.Results Statistical analysis showed that the mean optimal spatial frequency of grating stimuli that evoked the maximal response of V1 neurons in old cats was significantly lower than that in young adult cats.Furthermore,the mean high cut-off spatial frequency of grating stimuli that evoked the half amplitude of the maximal response of V1 neurons in old cats was also significantly lower than that in young adult cats.Conclusion These results are consistent with those reported in the V1 of old monkeys,suggesting that the age-related decline in the selectivity of visual cortical cells to spatial frequency could be generalized to all mammalian species and might contribute to visual acuity reduction in senescent individuals.展开更多
This study was conducted on the spatial distribution characteristics of surface tidal currents in the southwestern Taiwan Strait based on the quasi-harmonic analysis of current data obtained by two high frequency surf...This study was conducted on the spatial distribution characteristics of surface tidal currents in the southwestern Taiwan Strait based on the quasi-harmonic analysis of current data obtained by two high frequency surface wave radar(HFSWR) systems. The analysis shows that the tidal current pattern in the southwestern Taiwan Strait is primarily semi-diurnal and influenced significantly by shallow water constituents. The spatial distribution of tidal current ellipses of M2 is probably affected by the interaction between two different systems of tide wave, one from the northern mouth of Taiwan Strait and the other from the Bashi Channel. The directions of the major axes of M2 tidal current ellipses coincide roughly with the axis of the Taiwan Strait. The spatial distribution of the magnitudes of the probable maximum current velocity(PMCS) shows gradual increase of the velocity from northeast to southwest, which is in accordance with the spatial distribution of the measured maximum current velocity(MMCS). The directions of the residual currents are in accordance with the direction of the prevailing monsoon wind at the Taiwan Strait and the direction of the Taiwan warm current during summer. The bathymetry also shows a significant effect on the spatial distribution characteristics of tidal currents.展开更多
By the aftershock frequency estimation method based on the calculation of coseismic static Coulomb stress changes and rate-and state-dependent fault constitutive law,we calculate the frequency of "direct "af...By the aftershock frequency estimation method based on the calculation of coseismic static Coulomb stress changes and rate-and state-dependent fault constitutive law,we calculate the frequency of "direct "aftershocks of the Wenchuan earthquake related to coseismic static Coulomb stress changes in its aftershock zone and the areas nearby.It shows that the frequency is significantly lower than the truth in the main rupture zone,especially in the southern rupture zone,due to the decrease of stress level on the rupture plane of the main shock resulting from coseismic Coulomb stress change.The study also shows that the effect of the Coulomb stress change on the duration of aftershock activity is associated with the lower limit magnitude.The duration is about 15-16 months for aftershocks above ML4.0,and close to 60 months for aftershocks above ML3.5.In this period,the ratio of the"direct"aftershocks caused by coseismic Coulomb stress change ranges between 44.7% to48.6%,which suggests that,even in the "effective"period of coseismic Coulomb stress changes,about half of the aftershocks on the main shock rupture plane are independent of coseismic Coulomb stress changes.It is pointed out that those aftershocks may be related to the afterslip or the viscoelastic relaxation,which are time dependent cases.展开更多
Recently,there have been several uses for digital image processing.Image fusion has become a prominent application in the domain of imaging processing.To create one final image that provesmore informative and helpful ...Recently,there have been several uses for digital image processing.Image fusion has become a prominent application in the domain of imaging processing.To create one final image that provesmore informative and helpful compared to the original input images,image fusion merges two or more initial images of the same item.Image fusion aims to produce,enhance,and transform significant elements of the source images into combined images for the sake of human visual perception.Image fusion is commonly employed for feature extraction in smart robots,clinical imaging,audiovisual camera integration,manufacturing process monitoring,electronic circuit design,advanced device diagnostics,and intelligent assembly line robots,with image quality varying depending on application.The research paper presents various methods for merging images in spatial and frequency domains,including a blend of stable and curvelet transformations,everageMax-Min,weighted principal component analysis(PCA),HIS(Hue,Intensity,Saturation),wavelet transform,discrete cosine transform(DCT),dual-tree Complex Wavelet Transform(CWT),and multiple wavelet transform.Image fusion methods integrate data from several source images of an identical target,thereby enhancing information in an extremely efficient manner.More precisely,in imaging techniques,the depth of field constraint precludes images from focusing on every object,leading to the exclusion of certain characteristics.To tackle thess challanges,a very efficient multi-focus wavelet decomposition and recompositionmethod is proposed.The use of these wavelet decomposition and recomposition techniques enables this method to make use of existing optimized wavelet code and filter choice.The simulated outcomes provide evidence that the suggested approach initially extracts particular characteristics from images in order to accurately reflect the level of clarity portrayed in the original images.This study enhances the performance of the eXtreme Gradient Boosting(XGBoost)algorithm in detecting brain malignancies with greater precision through the integration of computational image analysis and feature selection.The performance of images is improved by segmenting them employing the K-Means algorithm.The segmentation method aids in identifying specific regions of interest,using Particle Swarm Optimization(PCA)for trait selection and XGBoost for data classification.Extensive trials confirm the model’s exceptional visual performance,achieving an accuracy of up to 97.067%and providing good objective indicators.展开更多
Background SCN1A is the most well-recognized and commonly mutated gene related to epilepsy.This study analyzed the characteristic spatial and frequency distributions of SCN1A mutations,aiming to provide important insi...Background SCN1A is the most well-recognized and commonly mutated gene related to epilepsy.This study analyzed the characteristic spatial and frequency distributions of SCN1A mutations,aiming to provide important insight into the mutagenesis etiopathology of SCN1A-associated epilepsy.Methods Epilepsy-associated SCN1A variants were retrieved from the SCN1A mutation database,the HGMD database,and literature reviews.The base substitutions,mutation frequencies in CpG dinucleotides,and spatial distributions of mutations in terms of exons and structural domains were analyzed.Results A total of 2621 SCN1A variants were identifed in 5106 unrelated cases.The most common type was missense mutation,followed by frameshift mutations and splice site mutations.Among the missense mutations,transitions within CpG dinucleotides were much more recurrently identifed than transitions within non-CpG dinucleotides,and the most common type was the G>A transition.Among the nonsense mutations,the most predominant type of single-base substitution was the C>T transition,among which 75.3%(235/312)were within CpG sites.The most common“hotspot”codons for missense mutations were codons 101,946,and 1783;while for nonsense mutations it was codon 712.One-base deletion or insertion was the most common type of frameshift mutation,causing protein truncation.The three most common frameshift mutations were c.5536_5539delAAAC,c.4554dupA,and c.5010_5013delGTTT.Splice mutations were the most frequently identifed in exon 4 with a hotspot site c.602+1G>A.The spatial distribution of missense mutations showed that exons 22 and 4 had the highest mutation density(111 and 84 mutations per 100 bp,respectively),and exon 12 had the lowest mutation density,with 4 mutations per 100 bp.Further distribution analysis of the protein domains revealed that missense mutations were more common in the pore region and voltage sensor(231 mutations per 100 amino acids,respectively),and the protein truncation mutations were distributed evenly among the domains.Conclusions SCN1A mutations tend to cluster at distinct sites,depending on the characteristic CpG dinucleotides,exons,and functional domains.Higher mutation density in particular regions,such as exon 22 and exon 4,ofers promising targets for therapeutic genetic interventions.展开更多
Bulk power systems show increasingly significant frequency spatial distribution characteristics(FSDCs),leading to a huge difference in the frequency response between regions.Existing uniform-frequency models based on ...Bulk power systems show increasingly significant frequency spatial distribution characteristics(FSDCs),leading to a huge difference in the frequency response between regions.Existing uniform-frequency models based on analytical methods are no longer applicable.This paper develops a reduced-order bus frequency response(BFR)model to preserve the FSDC and describe the frequency response of all buses.Its mathematical equation is proved to be isomorphic to the forced vibration of a mass-spring-damper system,and the closed-form solution(CFS)of the BFR model is derived by the modal analysis method and forced decoupling method in vibration mechanics.The correla tion between its mathematical equation and the state equation for small-signal stability analysis is discussed,and related pa rameters in the CFS are defined by the eigen-analysis method without any additional devices or tools.Case studies show that the proposed reduced-order BFR model and its CFS can im prove the solution accuracy while keeping the solution speed within milliseconds,which can preserve the significant FSDC of bulk power systems and represent a normalized mathematical description of distinct-frequency models.展开更多
The physiological characteristics of the marmoset second visual area(V2) are poorly understood compared with those of the primary visual area(V1). In this study, we observed the physiological response characteristics ...The physiological characteristics of the marmoset second visual area(V2) are poorly understood compared with those of the primary visual area(V1). In this study, we observed the physiological response characteristics of V2 neurons in four healthy adult marmosets using intracortical tungsten microelectrodes. We recorded 110 neurons in area V2, with receptive fields located between 8° and 15° eccentricity. Most(88.2%) of these neurons were orientation selective, with half-bandwidths typically ranging between 10° and 30°. A significant proportion of neurons(28.2%) with direction selectivity had a direction index greater than 0.5. The vast majority of V2 neurons had separable spatial frequency and temporal frequency curves and, according to this criterion, they were not speed selective. The basic functional response characteristics of neurons in area V2 resemble those found in area V1. Our findings show that area V2 together with V1 are important in primate visual processing, especially in locating objects in space and in detecting an object's direction of motion. The methods used in this study were approved by the Monash University Animal Ethics Committee, Australia(MARP 2009-2011) in 2009.展开更多
A scheme to improve the quality in ghost imaging(GI)by controlling the bandwidth of light source(BCGI)is proposed.The theoretical and numerical results show that the reconstruction result with high quality can be obta...A scheme to improve the quality in ghost imaging(GI)by controlling the bandwidth of light source(BCGI)is proposed.The theoretical and numerical results show that the reconstruction result with high quality can be obtained by adjusting the bandwidth range of the light source appropriately,and the selection criterion of the bandwidth is analyzed by the power distribution of the imaging target.A proof-of-principle experiment is implemented to verify the theoretical and numerical results.In addition,the BCGI also presents better anti-noise performance when compared with some popular GI methods.展开更多
In this paper, a method of predicting psychological values of color design was performed by using random color patterns. The results were analyzed in terms of the Fourier transform of the color patterns, and it was fo...In this paper, a method of predicting psychological values of color design was performed by using random color patterns. The results were analyzed in terms of the Fourier transform of the color patterns, and it was found that the psychological values of the random color patterns depended not only on the zero frequency component but also on the dynamic components of the Fourier transform of the patterns. The application of the estimation method was discussed.展开更多
Photodynamic therapy(PDT)dosimetry,induding light dose,photosensitizer dose and tissue oxygen,has been a research focus in PDT.In this work,we present a three-dimensional(3D)quantification of protoporphyrin X(PpIX)usi...Photodynamic therapy(PDT)dosimetry,induding light dose,photosensitizer dose and tissue oxygen,has been a research focus in PDT.In this work,we present a three-dimensional(3D)quantification of protoporphyrin X(PpIX)using combined spatial frequency domain imaging(SFDI)and diffuse fuorescence tomography(DFT).The SFDI maps both the distributions of tissue absorption and scattering properties at three wavelengths and accordingly provides the optical background for DFT and extracts the tissue oxygenation for assessing the therapeutic outcomes,while DFT dynamically monitors the 3D distribution of PpEX dose from measured fluorescence signals for the procedure optimization.A pilot in vrivo application in tumor nude models showed that the proposed SFDI/DFT is able to dynamically trace changes in the PpX concentration and tissue oaxygen during the treatment,rendering it a potentially powerful tool for PDT to improve clinical eficacy.展开更多
文摘We report a comprehensive numerical study of super resolution (SR) structured illumination microscopy (SIM) utilizing the classic Heintzmann-Cremer SIM process and algorithm. In particular, we investigated the impact of the diffraction limit of the underlying imaging system on the optimal SIM grating frequency that can be used to obtain the highest SR enhancement with non-continuous spatial frequency support. Besides confirming the previous theoretical and experimental work that SR-SIM can achieve an enhancement close to 3 times the diffraction limit with grating pattern illuminations, we also observe and report a series of more subtle effects of SR-SIM with non-continuous spatial frequency support. Our simulations show that when the SIM grating frequency exceeds twice that of the diffraction limit, the higher SIM grating frequency can help achieve a higher SR enhancement for the underlying imaging systems whose diffraction limit is low, though this enhancement is obtained at the cost of losing resolution at some lower resolution targets. Our simulations also show that, for underlying imaging systems with high diffraction limits, however, SR-SIM grating frequencies above twice the diffraction limits tend to bring no significant extra enhancement. Furthermore, we observed that there exists a limit grating frequency above which the SR enhancement effect is lost, and the reconstructed images essentially have the same resolution as the one obtained directly from the underlying imaging system without using the SIM process.
基金This research is supported by The Air Force Medical Center,China and in part of The Institute for Ultrafast Spectroscopy and Lasers(IUSL),the City College of the City University of New York.The authors would like to thank Mr.C.Y.Zhang,Mr.M.Z.Fan and Dr.X.H.Ni for their assistance in the experiments and suggestions concerning this paper.
文摘The purpose of this study is to examine optical spatial frequency spectroscopy analysis(SFSA)combined with visible resonance Raman(VRR)spectroscopic method,for thefirst time,to discriminate human brain metastases of lung cancers adenocarcinoma(ADC)and squamous cell carcinoma(SCC)from normal tissues.A total of 31 label-free micrographic images of three type of brain tissues were obtained using a confocal micro-Raman spectroscopic system.VRR spectra of the corresponding samples were synchronously collected using excitation wavelength of 532 nm from the same sites of the tissues.Using SFSA method,the difference in the randomness of spatial frequency structures in the micrograph images was analyzed using Gaussian functionfitting.The standard deviations,calculated from the spatial frequencies of the micrograph images were then analyzed using support vector machine(SVM)classifier.The key VRR biomolecularfingerprints of carotenoids,tryptophan,amide II,lipids and proteins(methylene/methyl groups)were also analyzed using SVM classifier.All three types of brain tissues were identified with high accuracy in the two approaches with high correlation.The results show that SFSA–VRR can potentially be a dual-modal method to provide new criteria for identifying the three types of human brain tissues,which are on-site,real-time and label-free and may improve the accuracy of brain biopsy.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61601442,61605218,and 61575207)the National Key Research and Development Program of China(Grant No.2018YFB0504302)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(Grant Nos.2015124 and 2019154)。
文摘Imaging quality is a critical component of compressive imaging in real applications. In this study, we propose a compressive imaging method based on multi-scale modulation and reconstruction in the spatial frequency domain. Theoretical analysis and simulation show the relation between the measurement matrix resolution and compressive sensing(CS)imaging quality. The matrix design is improved to provide multi-scale modulations, followed by individual reconstruction of images of different spatial frequencies. Compared with traditional single-scale CS imaging, the multi-scale method provides high quality imaging in both high and low frequencies, and effectively decreases the overall reconstruction error.Experimental results confirm the feasibility of this technique, especially at low sampling rate. The method may thus be helpful in promoting the implementation of compressive imaging in real applications.
基金supported by the National Natural Science Foundation of China(Grant Nos.12072191,51875343)Science and Technology Commission of Shanghai Municipality(Grant No.24DZ2307500).
文摘Electrical contact interfaces,integral to devices such as connectors,switches,and conductive slip rings,play a crucial role in determining current-carrying capabilities.This study adopts spatial frequency analysis to assess the currentcarrying performance of interfaces based on their morphological characteristics.Initially,rough surfaces with various parameters,including the cutoff frequency(N),the spectral index(β),and the gain factor(A),were generated using spatial frequency methods,followed by current-carrying simulations.The findings reveal that the characteristic parameters derived from spatial frequency methods exhibit a significantly stronger correlation with current-carrying performance than traditional roughness metrics.To enhance the practical applicability of this research,a physical-informed machine learning approach for rough surface features extraction was developed,demonstrating high predictive parameter identification accuracy for the three parameters.Overall,this paper introduces a tribo-informatics approach for analyzing and extracting rough surface features that considers the curvature characteristics of contact points.This approach holds significant potential for evaluating the processing state of current-carrying interfaces,selecting high-quality surfaces,and forecasting current-carrying performances.
基金supports from the National Natural Science Foundation of China(12074123,12174108)the Foundation of‘Manufacturing beyond limits’of Shanghai‘Talent Program'of Henan Academy of Sciences.
文摘Femtosecond laser-induced periodic surface structures(LIPSS)have been extensively studied over the past few decades.In particular,the period and groove width of high-spatial-frequency LIPSS(HSFL)is much smaller than the diffraction limit,making it a useful method for efficient nanomanufacturing.However,compared with the low-spatial-frequency LIPSS(LSFL),the structure size of the HSFL is smaller,and it is more easily submerged.Therefore,the formation mechanism of HSFL is complex and has always been a research hotspot in this field.In this study,regular LSFL with a period of 760 nm was fabricated in advance on a silicon surface with two-beam interference using an 800 nm,50 fs femtosecond laser.The ultrafast dynamics of HSFL formation on the silicon surface of prefabricated LSFL under single femtosecond laser pulse irradiation were observed and analyzed for the first time using collinear pump-probe imaging method.In general,the evolution of the surface structure undergoes five sequential stages:the LSFL begins to split,becomes uniform HSFL,degenerates into an irregular LSFL,undergoes secondary splitting into a weakly uniform HSFL,and evolves into an irregular LSFL or is submerged.The results indicate that the local enhancement of the submerged nanocavity,or the nanoplasma,in the prefabricated LSFL ridge led to the splitting of the LSFL,and the thermodynamic effect drove the homogenization of the splitting LSFL,which evolved into HSFL.
基金supported by the National Key R&D Program of China(2020YFA0714500)the National Natural Science Youth Foundation of China(62205352)+5 种基金the Member of Youth Innovation Promotion Association of the Chinese Academy of Sciencesthe International Partnership Program of the Chinese Academy of Sciences(181231KYSB20200040)the Chinese Academy of Sciences President’s International Fellowship Initiative(2023VMB0008)the Shanghai Sailing Program(20YF1454800)the Natural Science Foundation of Shanghai(21ZR1472000)the Shanghai Strategic Emerging Industry Development Special Fund(31011442501217020191D3101001)。
文摘The large-aperture pulse compression grating(PCG) is a critical component in generating an ultra-high-intensity, ultra-short-pulse laser;however, the size of the PCG manufactured by transmission holographic exposure is limited to large-scale high-quality materials. The reflective method is a potential way for solving the size limitation, but there is still no successful precedent due to the lack of scientific specifications and advanced processing technology of exposure mirrors. In this paper, an analytical model is developed to clarify the specifications of components, and advanced processing technology is adopted to control the spatial frequency errors. Hereafter, we have successfully fabricated a multilayer dielectric grating of 200 mm × 150 mm by using an off-axis reflective exposure system with Φ300 mm. This demonstration proves that PCGs can be manufactured by using the reflection holographic exposure method and shows the potential for manufacturing the meter-level gratings used in 100 petawatt class high-power lasers.
基金supported by The Guangdong Province Agricultural Science and Technology Innovation and Promotion Project(No.2020KJ102,No.2021KJ102)Natural Science Foundation of Guangdong Province(2021A1515011643).
文摘Cultivated land quality(CLQ)is related to national food security.Rapid and high-precision monitoring of CLQ is crucial for the sustainable development of agriculture.However,current satellite image-based evaluation methods that only consider the crop's spatial spectrum characteristics in the key growth stages cannot accurately estimate CLQ.This study proposes a new method based on time-series spectral data of crop growth to improve the accuracy of CLQ estimation.This study was conducted in the Conghua District of Guangzhou,Guangdong Province,China.The results showed that seven spectral indicators were determined as the optimal indicators based on the gradient boosting decision tree(GBDT)and variance inflation factor(VIF).And the genetic algorithm-back propagation neural network(GA-BPNN)model provided more accurate CLQ estimates than the partial least squares regression(PLSR)model,indicating a nonlinear relationship between CLQ and the indicators.In addition,the GA-BPNN model with a normalized root mean square error(NRMSE)of 9.91%demonstrates the excellent potential for mapping CLQ over large areas.The model based on the seven optimal indicators of crop phenology provided higher accuracy than the GA-BPNN model based on the normalized difference vegetation index(NDVI)indicators in the spatial domain,significantly decreasing the NRMSE of the CLQ estimates by 3.17%.This further implied that the spectral indicators in the spatial frequency domain can improve the accuracy of estimating CLQ.
文摘A technique for coherent imaging based on spatial frequency heterodyning is described. Three images corresponding to three physical measurements are recorded. For the first measurement, a scene is simply illuminated with a coherent beam and for measurements 2 and 3, the scene is projected with cosine and sine fringes, respectively. Due to spatial frequency heterodyning, upper and lower side hand information falls in the pass band of the imager. These bands are separated and correct phases and positions are assigned to these bands in the spatial frequency domain. An extension of bandwidth is achieved in the frequency domain and the inverse frequency domain data then give a high resolution coherent image.
基金the National Natural Science Foundation of China(Grant Nos.61735017,61822510,62020106002,61905097,and 62005250)the Zhejiang Provincial Natural Science of China(Grant No.LR17F050002)the Zhejiang University Education Foundation Global Partnership Fund.
文摘Spatial frequency shift(SFS) microscopy with evanescent wave illumination shows intriguing advantages, including large field of view(FOV), high speed, and good modularity. However, a missing band in the spatial frequency domain hampers the SFS superresolution microscopy from achieving resolution better than 3 folds of the Abbe diffraction limit. Here, we propose a novel tunable large-SFS microscopy, making the resolution improvement of a linear system no longer restricted by the detection numerical aperture(NA). The complete wide-range detection in the spatial frequency domain is realized by tuning the illumination spatial frequency actively and broadly through an angle modulation between the azimuthal propagating directions of two evanescent waves. The vertical spatial frequency is tuned via a sectional saturation effect, and the reconstructed depth information can be added to the lateral superresolution mask for 3D imaging. A lateral resolution of λ/9, and a vertical localization precision of ~λ/200(detection objective NA = 0.9) are realized with a gallium phosphide(GaP) waveguide. Its unlimited resolution enhancing capability is demonstrated by introducing a designed metamaterial chip with an unusual large refractive index. Besides the great resolution enhancement, this method shows better anti-noise capability than classical structured illumination microscopy without SFS tunability. This method is chip-compatible and can potentially provide a massproducible illumination chip module achieving the fast, large-FOV, and deep-subwavelength 3D nanoscopy.
基金China Postdoctoral Science Foundation Under Grant No. 2004035215 Jiangsu Planned Projects for Postdoctoral Research Funds 2004 Aeronautical Science Research Foundation Under Grant No. 04152065
文摘A frequency and spatial domain decomposition method （FSDD） for operational modal analysis （OMA） is presented in this paper, which is an extension of the complex mode indicator function （CMIF） method for experimental modal analysis （EMA）. The theoretical background of the FSDD method is clarified, Singular value decomposition is adopted to separate the signal space from the noise space. Finally, an enhanced power spectrum density （PSD） is proposed to obtain more accurate modal parameters by curve fitting in the frequency domain. Moreover, a simulation case and an application case are used to validate this method.
基金supported by Natural Science Foundation of Anhui Province(No. 070413138)the Key Research Foundation of Education Department of Anhui Province,China(No. KJ2009A167)
文摘Objective To examine whether the selectivity of visual cortical neurons to stimulus spatial frequencies would be affected by aging in cats.Methods In vivo extracellular single-unit recording techniques were employed to record the tuning responses of V1 neurons to different stimulus spatial frequencies in old and young adult cats.Results Statistical analysis showed that the mean optimal spatial frequency of grating stimuli that evoked the maximal response of V1 neurons in old cats was significantly lower than that in young adult cats.Furthermore,the mean high cut-off spatial frequency of grating stimuli that evoked the half amplitude of the maximal response of V1 neurons in old cats was also significantly lower than that in young adult cats.Conclusion These results are consistent with those reported in the V1 of old monkeys,suggesting that the age-related decline in the selectivity of visual cortical cells to spatial frequency could be generalized to all mammalian species and might contribute to visual acuity reduction in senescent individuals.
基金supported by the National High Technology Research and Development Program (‘863’ Program) of China under contract No. 2012AA091701the Fundamental Research Fund for the Central University of China under the contract No. 2012212020211
文摘This study was conducted on the spatial distribution characteristics of surface tidal currents in the southwestern Taiwan Strait based on the quasi-harmonic analysis of current data obtained by two high frequency surface wave radar(HFSWR) systems. The analysis shows that the tidal current pattern in the southwestern Taiwan Strait is primarily semi-diurnal and influenced significantly by shallow water constituents. The spatial distribution of tidal current ellipses of M2 is probably affected by the interaction between two different systems of tide wave, one from the northern mouth of Taiwan Strait and the other from the Bashi Channel. The directions of the major axes of M2 tidal current ellipses coincide roughly with the axis of the Taiwan Strait. The spatial distribution of the magnitudes of the probable maximum current velocity(PMCS) shows gradual increase of the velocity from northeast to southwest, which is in accordance with the spatial distribution of the measured maximum current velocity(MMCS). The directions of the residual currents are in accordance with the direction of the prevailing monsoon wind at the Taiwan Strait and the direction of the Taiwan warm current during summer. The bathymetry also shows a significant effect on the spatial distribution characteristics of tidal currents.
基金funded by the special project of the National Science&Technology Pillar Program during the Twelfth "Five-year Plan" Period(2012BAK19B02-05-02)
文摘By the aftershock frequency estimation method based on the calculation of coseismic static Coulomb stress changes and rate-and state-dependent fault constitutive law,we calculate the frequency of "direct "aftershocks of the Wenchuan earthquake related to coseismic static Coulomb stress changes in its aftershock zone and the areas nearby.It shows that the frequency is significantly lower than the truth in the main rupture zone,especially in the southern rupture zone,due to the decrease of stress level on the rupture plane of the main shock resulting from coseismic Coulomb stress change.The study also shows that the effect of the Coulomb stress change on the duration of aftershock activity is associated with the lower limit magnitude.The duration is about 15-16 months for aftershocks above ML4.0,and close to 60 months for aftershocks above ML3.5.In this period,the ratio of the"direct"aftershocks caused by coseismic Coulomb stress change ranges between 44.7% to48.6%,which suggests that,even in the "effective"period of coseismic Coulomb stress changes,about half of the aftershocks on the main shock rupture plane are independent of coseismic Coulomb stress changes.It is pointed out that those aftershocks may be related to the afterslip or the viscoelastic relaxation,which are time dependent cases.
基金Princess Nourah bint Abdulrahman University and Researchers Supporting Project Number(PNURSP2024R346)Princess Nourah bint Abdulrahman University,Riyadh,Saudi Arabia.
文摘Recently,there have been several uses for digital image processing.Image fusion has become a prominent application in the domain of imaging processing.To create one final image that provesmore informative and helpful compared to the original input images,image fusion merges two or more initial images of the same item.Image fusion aims to produce,enhance,and transform significant elements of the source images into combined images for the sake of human visual perception.Image fusion is commonly employed for feature extraction in smart robots,clinical imaging,audiovisual camera integration,manufacturing process monitoring,electronic circuit design,advanced device diagnostics,and intelligent assembly line robots,with image quality varying depending on application.The research paper presents various methods for merging images in spatial and frequency domains,including a blend of stable and curvelet transformations,everageMax-Min,weighted principal component analysis(PCA),HIS(Hue,Intensity,Saturation),wavelet transform,discrete cosine transform(DCT),dual-tree Complex Wavelet Transform(CWT),and multiple wavelet transform.Image fusion methods integrate data from several source images of an identical target,thereby enhancing information in an extremely efficient manner.More precisely,in imaging techniques,the depth of field constraint precludes images from focusing on every object,leading to the exclusion of certain characteristics.To tackle thess challanges,a very efficient multi-focus wavelet decomposition and recompositionmethod is proposed.The use of these wavelet decomposition and recomposition techniques enables this method to make use of existing optimized wavelet code and filter choice.The simulated outcomes provide evidence that the suggested approach initially extracts particular characteristics from images in order to accurately reflect the level of clarity portrayed in the original images.This study enhances the performance of the eXtreme Gradient Boosting(XGBoost)algorithm in detecting brain malignancies with greater precision through the integration of computational image analysis and feature selection.The performance of images is improved by segmenting them employing the K-Means algorithm.The segmentation method aids in identifying specific regions of interest,using Particle Swarm Optimization(PCA)for trait selection and XGBoost for data classification.Extensive trials confirm the model’s exceptional visual performance,achieving an accuracy of up to 97.067%and providing good objective indicators.
基金funded by Research Foundation of China Association Against Epilepsy(CU-2024-042)Medical Scientifc Research Foundation of Guangdong Province(A2024536)+1 种基金the National Natural Science Foundation of China(Grant No.82071548,Grant No.82301639 and Grant No.82201609)Guangzhou Medical University Student Innovation Ability Promoting Program(Grant No.2022A045 and Grant No.02-408-240603131135).
文摘Background SCN1A is the most well-recognized and commonly mutated gene related to epilepsy.This study analyzed the characteristic spatial and frequency distributions of SCN1A mutations,aiming to provide important insight into the mutagenesis etiopathology of SCN1A-associated epilepsy.Methods Epilepsy-associated SCN1A variants were retrieved from the SCN1A mutation database,the HGMD database,and literature reviews.The base substitutions,mutation frequencies in CpG dinucleotides,and spatial distributions of mutations in terms of exons and structural domains were analyzed.Results A total of 2621 SCN1A variants were identifed in 5106 unrelated cases.The most common type was missense mutation,followed by frameshift mutations and splice site mutations.Among the missense mutations,transitions within CpG dinucleotides were much more recurrently identifed than transitions within non-CpG dinucleotides,and the most common type was the G>A transition.Among the nonsense mutations,the most predominant type of single-base substitution was the C>T transition,among which 75.3%(235/312)were within CpG sites.The most common“hotspot”codons for missense mutations were codons 101,946,and 1783;while for nonsense mutations it was codon 712.One-base deletion or insertion was the most common type of frameshift mutation,causing protein truncation.The three most common frameshift mutations were c.5536_5539delAAAC,c.4554dupA,and c.5010_5013delGTTT.Splice mutations were the most frequently identifed in exon 4 with a hotspot site c.602+1G>A.The spatial distribution of missense mutations showed that exons 22 and 4 had the highest mutation density(111 and 84 mutations per 100 bp,respectively),and exon 12 had the lowest mutation density,with 4 mutations per 100 bp.Further distribution analysis of the protein domains revealed that missense mutations were more common in the pore region and voltage sensor(231 mutations per 100 amino acids,respectively),and the protein truncation mutations were distributed evenly among the domains.Conclusions SCN1A mutations tend to cluster at distinct sites,depending on the characteristic CpG dinucleotides,exons,and functional domains.Higher mutation density in particular regions,such as exon 22 and exon 4,ofers promising targets for therapeutic genetic interventions.
基金supported in part by the National Natural Science Foundation of China(No.U22A20223).
文摘Bulk power systems show increasingly significant frequency spatial distribution characteristics(FSDCs),leading to a huge difference in the frequency response between regions.Existing uniform-frequency models based on analytical methods are no longer applicable.This paper develops a reduced-order bus frequency response(BFR)model to preserve the FSDC and describe the frequency response of all buses.Its mathematical equation is proved to be isomorphic to the forced vibration of a mass-spring-damper system,and the closed-form solution(CFS)of the BFR model is derived by the modal analysis method and forced decoupling method in vibration mechanics.The correla tion between its mathematical equation and the state equation for small-signal stability analysis is discussed,and related pa rameters in the CFS are defined by the eigen-analysis method without any additional devices or tools.Case studies show that the proposed reduced-order BFR model and its CFS can im prove the solution accuracy while keeping the solution speed within milliseconds,which can preserve the significant FSDC of bulk power systems and represent a normalized mathematical description of distinct-frequency models.
基金supported by travel grants from Monash University and the University of Sichuan(to YY)Research Grants from the Australian Research Council(No.DP0451206)(to MGPR)National Health and Medical Research Council(No.384115)(to MGPR)。
文摘The physiological characteristics of the marmoset second visual area(V2) are poorly understood compared with those of the primary visual area(V1). In this study, we observed the physiological response characteristics of V2 neurons in four healthy adult marmosets using intracortical tungsten microelectrodes. We recorded 110 neurons in area V2, with receptive fields located between 8° and 15° eccentricity. Most(88.2%) of these neurons were orientation selective, with half-bandwidths typically ranging between 10° and 30°. A significant proportion of neurons(28.2%) with direction selectivity had a direction index greater than 0.5. The vast majority of V2 neurons had separable spatial frequency and temporal frequency curves and, according to this criterion, they were not speed selective. The basic functional response characteristics of neurons in area V2 resemble those found in area V1. Our findings show that area V2 together with V1 are important in primate visual processing, especially in locating objects in space and in detecting an object's direction of motion. The methods used in this study were approved by the Monash University Animal Ethics Committee, Australia(MARP 2009-2011) in 2009.
基金the National Natural Science Foundation of China(Grant Nos.61871431,61971184,and 62001162).
文摘A scheme to improve the quality in ghost imaging(GI)by controlling the bandwidth of light source(BCGI)is proposed.The theoretical and numerical results show that the reconstruction result with high quality can be obtained by adjusting the bandwidth range of the light source appropriately,and the selection criterion of the bandwidth is analyzed by the power distribution of the imaging target.A proof-of-principle experiment is implemented to verify the theoretical and numerical results.In addition,the BCGI also presents better anti-noise performance when compared with some popular GI methods.
文摘In this paper, a method of predicting psychological values of color design was performed by using random color patterns. The results were analyzed in terms of the Fourier transform of the color patterns, and it was found that the psychological values of the random color patterns depended not only on the zero frequency component but also on the dynamic components of the Fourier transform of the patterns. The application of the estimation method was discussed.
基金supported by the National Natural Science Foundation of China under Grant Nos.(81871393 and 62075156).
文摘Photodynamic therapy(PDT)dosimetry,induding light dose,photosensitizer dose and tissue oxygen,has been a research focus in PDT.In this work,we present a three-dimensional(3D)quantification of protoporphyrin X(PpIX)using combined spatial frequency domain imaging(SFDI)and diffuse fuorescence tomography(DFT).The SFDI maps both the distributions of tissue absorption and scattering properties at three wavelengths and accordingly provides the optical background for DFT and extracts the tissue oxygenation for assessing the therapeutic outcomes,while DFT dynamically monitors the 3D distribution of PpEX dose from measured fluorescence signals for the procedure optimization.A pilot in vrivo application in tumor nude models showed that the proposed SFDI/DFT is able to dynamically trace changes in the PpX concentration and tissue oaxygen during the treatment,rendering it a potentially powerful tool for PDT to improve clinical eficacy.
