Background:Mastitis seriously affects the mammary health of humans and animals.Studies have found that inflammation and oxidative stress play key roles in the occur-rence and development of mastitis.Therefore,in-depth...Background:Mastitis seriously affects the mammary health of humans and animals.Studies have found that inflammation and oxidative stress play key roles in the occur-rence and development of mastitis.Therefore,in-depth research on related molecular mechanisms is of great significance.Methods:Postpartum mice were anesthetized with pentobarbital and administered lipopolysaccharide to develop the mouse mastitis model.Proteomic analysis was per-formed to compare protein expression in mitochondria-associated endoplasmic retic-ulum membranes(MAM)from two mouse mammary gland groups.Western blot was used to detect the expression of MAM-related proteins in mitochondria.AlphaFold3 was used to predict the molecular structures of phosphofurin acidic cluster sorting protein 2(PACS2)and mitofusin 2(MFN2)and their interaction levels.The MFN2-PACS2 interaction was investigated using co-immunoprecipitation and small interfer-ing RNA.Results:The results showed that the inflammation level in the mammary gland tissue of mice with mastitis significantly increased,the total antioxidant capacity decreased,and the expression of MAM-related proteins MFN2 and PACS2 was significantly downregulated.In cell experiments,overexpression of MFN2 can inhibit inflamma-tion and oxidative stress responses,and promote the interaction between MFN2 and PACS2 to affect the formation of MAMs.Conclusion:In summary,this study suggests that mastitis can alter the expression of MAM-related proteins in mouse breast tissue.The interaction between MFN2 and PACS2 regulates the formation of MAMs.Overexpression of MFN2 can promote the formation of MAMs and inhibit inflammation and oxidative stress response in mam-mary epithelial cells.Our results provided a new theoretical basis and potential thera-peutic targets for the prevention and treatment of mastitis.展开更多
AIM： To develop a method to isolate liver stem cells fast and efficiently. METHODS： Fetal mouse liver cells were characterized by cell surface antigens （c-Kit and CD45/TER119） using flow cytometry. The candidate l...AIM： To develop a method to isolate liver stem cells fast and efficiently. METHODS： Fetal mouse liver cells were characterized by cell surface antigens （c-Kit and CD45/TER119） using flow cytometry. The candidate liver stem cells were sorted by using immuno-magnetic microbeads and identified by -lone-forming culture, RT-PCR and immunofluorescence says. RESULTS： The c-Kit-（CD45/TER119）- cell population with 97.9% of purity were purified by immuno-magnetic microbeads at one time. The yield of this separation was about 6% of the total sorting cells and the cell viability was above 98%. When cultured in vitro these cells had high clone-forming and self-renewing ability and expressed markers of hepatocytes and bile duct cells. Functionally mature hepatocytes were observed after 21 d of culture. CONCLUSION： This method offers an excellent tool for the enrichment of liver stem cells with high purity and viability, which could be used for further studies. It is fast, efficient, simple and not expensive.展开更多
Erianthus arundinaceus is a wild relative of sugarcane(Saccharum officinarum L.)with many desirable agronomic traits for sugarcane genetic improvement.However,limited knowledge of the complex genome of hexaploid E.aru...Erianthus arundinaceus is a wild relative of sugarcane(Saccharum officinarum L.)with many desirable agronomic traits for sugarcane genetic improvement.However,limited knowledge of the complex genome of hexaploid E.arundinaceus has impeded the development of required molecular tools.Dissecting complex genomes into single chromosomes can simplify analyses.The flow-cytometric sorting of a single chromosome of E.arundinaceus in a Saccharum-Erianthus introgression line is reported.A novel approach called genomic in situ hybridization in suspension was used to discriminate the alien chromosome from sugarcane chromosomes at the same size.A total of 218,000 E.arundinaceus chromosome 1(EaC1)were sorted to>97%purity and amplified DNA was sequenced using Illumina and Pac Bio technologies.The resulting assembly had a 70.93 Mb contig sequence with an N50 of 19.62 kb.A total of 56.69 Mb repeat sequences were predicted,accounting for 79.1%of the chromosome and 2646 genes having a total length of 1.84 Mb that represented 2.59%of the chromosome.Of these genes,1877(70.9%)genes were functionally annotated.The phylogenetic relationship of E.arundinaceus with other species using the chromosome1 sequence revealed that E.arundinaceus was distantly related to Oryza sativa and Zea mays,followed by Sorghum bicolor,and was closely related to S.spontaneum and Saccharum spp.hybrids.This study provides the first insights into the characteristics of EaC1,and the results will provide tools to support molecular improvement and alien introgression breeding of sugarcane.展开更多
PC12 cell injury was induced using 20 μM amyloid β-protein 25-35 to establish a model of Alzheimer＇s disease. The cells were then treated with 5, 10, and 25 μM Schisandrin B. Methylthiazolyldiphenyl-tetrazolium br...PC12 cell injury was induced using 20 μM amyloid β-protein 25-35 to establish a model of Alzheimer＇s disease. The cells were then treated with 5, 10, and 25 μM Schisandrin B. Methylthiazolyldiphenyl-tetrazolium bromide assays and Hoechst 33342 staining results showed that with increasing Schisandrin B concentration, the survival rate of PC12 cells injured by amyloid β-protein 25-35 gradually increased and the rate of apoptosis gradually decreased. Reverse transcription-PCR, immunocytochemical staining and western blot results showed that with increasing Schisandrin B concentration, the mRNA and protein expression of vacuolar protein sorting 35 and amyloid precursor protein were gradually decreased. Vacuolar protein sorting 35 and amyloid precursor protein showed a consistent trend for change. These findings suggest that 5, 10, and 25 μM Schisandrin B antagonizes the cellular injury induced by amyloid β-protein 25-35 in a dose-dependent manner. This may be caused by decreasing the expression of vacuolar protein sorting 35 and amyloid precursor protein.展开更多
The transition of traits between genetically related lineages is a fascinating topic that provides clues to understanding the drivers of speciation and diversification.Much can be learned about this process from phylo...The transition of traits between genetically related lineages is a fascinating topic that provides clues to understanding the drivers of speciation and diversification.Much can be learned about this process from phylogeny-based trait evolution.However,such inference is often plagued by genome-wide gene-tree discordance(GTD),mostly due to incomplete lineage sorting(ILS)and/or introgressive hybridization,especially when the genes underlying the traits appear discordant.Here,by collecting transcriptomes,whole chloroplast genomes(cpDNA),and population genetic datasets,we used the coalescent model to turn GTD into a source of information for ILS and employed hemiplasy to explain specific cases of apparent“phylogenetic discordance”between different morphological traits and probable species phylogeny in the Allium subg.Cyathophora.Both concatenation and coalescence methods consistently showed the same phylogenetic topology for species tree inference based on single-copy genes(SCGs),as supported by the KS distribution.However,GTD was high across the genomes of subg.Cyathophora:~27%e38.9%of the SCG trees were in conflict with the species tree.Plasmid and nuclear incongruence was also present.Our coalescent simulations indicated that such GTD was mainly a product of ILS.Our hemiplasy risk factor calculations supported that random fixation of ancient polymorphisms in different populations during successive speciation events along the subg.Cyathophora phylogeny may have caused the character transition,as well as the anomalous cpDNA tree.Our study exemplifies how phylogenetic noise can be transformed into evolutionary information for understanding character state transitions along species phylogenies.展开更多
AIM TO detect significant clusters of co-expressed genes associated with tumorigenesis that might help to predict stomach adenocarcinoma （SA） prognosis.METHODS The Cancer Genome Atlas database was used to obtain RNA...AIM TO detect significant clusters of co-expressed genes associated with tumorigenesis that might help to predict stomach adenocarcinoma （SA） prognosis.METHODS The Cancer Genome Atlas database was used to obtain RNA sequences as well as complete clinical data of SA and adjacent normal tissues from patients. Weighted gene co-expression network analysis （WGCNA） was used to investigate the meaningful module along with hub genes. Expression of hub genes was analyzed in 362 paraffin-embedded SA biopsy tissues by immunohistochemical staining. Patients were classified into two groups （according to expression of hub genes）： Weak expression and over-expression groups. Correlation of biomarkers with clinicopathological factors indicated patient survival.RESULTS Whole genome expression level screening identified 6,231 differentially expressed genes. Twenty-four co- expressed gene modules were identified using WGCNA. Pearson＇s correlation analysis showed that the tan module was the most relevant to tumor stage （r = 0.24, P = 7 × 10 -6）. In addition, we detected sorting nexin （SNX）10 as the hub gene of the tan module. SNX10 expression was linked to T category （P = 0.042, x2= 8.708）, N category （P = 0.000, x2= 18.778）, TNM stage （P = 0.001, x2 = 16.744） as well as tumor differentiation （P = 0.000,x2= 251.930）. Patients with high SNX10 expression tended to have longer diseasefree survival （DFS; 44.97 mo vs 33.85 mo, P = 0.000） as well as overall survival （OS; 49.95 vs 40.84 mo, P = 0.000） in univariate analysis. Multivariate analysis showed that dismal prognosis could be precisely predicted clinicopathologically using SNX10 [DFS： P = 0.014, hazard ratio （HR） = 0.698, 95% confidence interval （CI）： 0.524-0.930, OS： P = 0.017, HR = 0.704, 95%CI： 0.528-0.940].CONCLUSION This study provides a new technique for screening prognostic biomarkers of SA. Weak expression of SNX10 is linked to poor prognosis, and is a suitable prognostic biomarker of SA.展开更多
Using a high-gradient magnetic cell sorting (MACS) system, CD34^+ cells were isolated from human bone marrow, cord blood, peripheral blood, and cultured in CFU system. The results showed that the excellent recovery (7...Using a high-gradient magnetic cell sorting (MACS) system, CD34^+ cells were isolated from human bone marrow, cord blood, peripheral blood, and cultured in CFU system. The results showed that the excellent recovery (75%) and highest purity (95-99%) were acquired by using the MACS separation system, and the most CFCs were present in CD34^+ population, but not in CD34 fraction by clonogenic assays. The isolation of pure hematopoietic stem cells and progenitor cells is of experimental and clinical importance.展开更多
Heterogeneity of biological samples is usually considered a major obstacle for three-dimensional (3D) structure determination of macromolecular complexes. Heterogeneity may occur at the level of composition or conform...Heterogeneity of biological samples is usually considered a major obstacle for three-dimensional (3D) structure determination of macromolecular complexes. Heterogeneity may occur at the level of composition or conformational variability of complexes and affects most 3D structure determination methods that rely on signal averaging. Here, an approach is described that allows sorting structural states based on a 3D statistical approach, the 3D sampling and classification (3D-SC) of 3D structures derived from single particles imaged by cryo electron microscopy (cryo-EM). The method is based on jackknifing & bootstrapping of 3D sub-ensembles and 3D multivariate statistical analysis followed by 3D classification. The robustness of the statistical sorting procedure is corroborated using model data from an RNA polymerase structure and experimental data from a ribosome complex. It allows resolving multiple states within heterogeneous complexes that thus become amendable for a structural analysis despite of their highly flexible nature. The method has important implications for high-resolution structural studies and allows describing structure ensembles to provide insights into the dynamics of multi-component macromolecular assemblies.展开更多
Several parallel sorting techniques on different architectures have been studied for many years. Due to the need for faster systems in today＇s world, parallelism can be used to accelerate applications. Nowadays, para...Several parallel sorting techniques on different architectures have been studied for many years. Due to the need for faster systems in today＇s world, parallelism can be used to accelerate applications. Nowadays, parallel operations are used to solve computer problems such as sort and search, which result in a reasonable speed. Sorting is one of the most important operations in computing world. The authors always try to find the best in different areas which the premier is speedup. In this paper, the authors issued a sort with O（logn） time complexity on PRAM EREW （Parallel Random Access Machine Exclusive Read Exclusive Write）. The algorithm is designed in a manner that keeps the tradeoff between the number of processor elements in the architecture and execution time. The simulation of the algorithm proves the theoretical analysis of the algorithm. The results of this research can be utilized in developing faster embedded systems. Sorting on Centralized Diamond （SOCD） algorithm is issued on the novel Centralized Diamond architecture which takes the advantages of Single Instruction Multiple Data （SIMD） architecture. This architecture and the sort on it are intuitive and optimal.展开更多
Proteolytic processing of the transmembrane amyloid precursor protein (APP) to aggregation-prone amyloid-β (Aβ) peptide underlies the development of Alzheimer’s disease.
The somatotopic representation of specific body parts is a well-established spatial organizational principle in the primary somatosensory and motor cortices.
The ongoing coronavirus epidemic,including the novel coronavirus(SARS-CoV-2),continues to pose a significant threat to global public health.Host targets address multiple stages of the viral life cycle and provide dive...The ongoing coronavirus epidemic,including the novel coronavirus(SARS-CoV-2),continues to pose a significant threat to global public health.Host targets address multiple stages of the viral life cycle and provide diverse opportunities for therapeutic interventions.This study identified sorting nexin 10(SNX10)as a facilitator of replication of human coronavirus OC43(HCoV-OC43),underscoring its potential as a novel antiviral target.The knockout of SNX10 significantly suppressed HCoV-OC43 replication both in vivo and in vitro.Immunoprecipitation-mass spectrometry(IP-MS)analysis identified the adaptor protein complex 2 subunitμ1(AP2M1)as a direct interactor of SNX10.Specifically,SNX10 facilitates phosphorylation of the AP2M1,thereby enhancing clathrin-mediated viral endocytosis.Furthermore,subsequent binding and internalization assays revealed that SNX10 knockout significantly inhibits viral entry into host cells.Conversely,the reconstitution of SNX10 fully restored viral entry,thereby confirming the critical and indispensable role of SNX10 in pathogen internalization.Simultaneously,SNX10 was identified as a key factor that promotes endosomal acidification by modulating pH levels,which in turn facilitated the release of the viral genome.Notably,the ablation of SNX10 was found to trigger autophagy activation during infection,thereby maintaining intracellular homeostasis.Additionally,it exerted autonomous antiviral effects through lysosomal degradation pathways.Collectively,these findings demonstrate SNX10 serves as a pivotal regulator of the viral life cycle and underscore its therapeutic potential as a multi-faceted antiviral candidate target capable of simultaneously inhibiting viral internalization,viral genomic release,and hostpathogen equilibrium.展开更多
This paper introduces an intelligent garbage-handling trolley model based on an STM32 single chip microcomputer as the control core.The device is driven by four independent motors to achieve automatic tracking,automat...This paper introduces an intelligent garbage-handling trolley model based on an STM32 single chip microcomputer as the control core.The device is driven by four independent motors to achieve automatic tracking,automatic obstacle avoidance,and fixed-point docking.Using external execution structure to realize the car without the use of a mechanical arm,complete garbage collection,storage,and uninstall function.On this basis,the type of garbage is marked by color,and the color recognition sensor is applied to realize the type recognition after garbage collection and put into the corresponding unloading point,to realize its intelligent classification function.It can automatically complete the established task autonomously.展开更多
The progress of modern industry has given rise to great requirements for network transmission latency and reliability in domains such as smart grid and intelligent driving.To address these challenges,the concept of Ti...The progress of modern industry has given rise to great requirements for network transmission latency and reliability in domains such as smart grid and intelligent driving.To address these challenges,the concept of Time-sensitive networking(TSN)is proposed by IEEE 802.1TSN working group.In order to achieve low latency,Cyclic queuing and forwarding(CQF)mechanism is introduced to schedule Timetriggered(TT)flows.In this paper,we construct a TSN model based on CQF and formulate the flow scheduling problem as an optimization problem aimed at maximizing the success rate of flow scheduling.The problem is tackled by a novel algorithm that makes full use of the characteristics and the relationship between the flows.Firstly,by K-means algorithm,the flows are initially partitioned into subsets based on their correlations.Subsequently,the flows within each subset are sorted by a new special criteria extracted from multiple features of flow.Finally,a flow offset selecting method based on load balance is used for resource mapping,so as to complete the process of flow scheduling.Experimental results demonstrate that the proposed algorithm exhibits significant advantages in terms of scheduling success rate and time efficiency.展开更多
Manned aerial vehicle-unmanned aerial vehicle(MAV-UAV)combat organization is a MAV-UAV combat collective formed from the perspective of organization design theory and methodology,and the generation of force formation ...Manned aerial vehicle-unmanned aerial vehicle(MAV-UAV)combat organization is a MAV-UAV combat collective formed from the perspective of organization design theory and methodology,and the generation of force formation plan is a key step in the organizational planning.Based on the description of the problem and the definition of organizational elements,the matching model of platform-target attack wave is constructed to minimize the redundancy of command and decision-making capability,resource capability and the number of platforms used.Based on the non-dominated sorting genetic algorithmⅢ(NSGA-Ⅲ)framework,which includes encoding/decoding method and constraint handling method,the generation model of organizational force formation plan is solved,and the effectiveness and superiority of the algorithm are verified by simulation experiments.展开更多
Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established fro...Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome painting was used to characterize karyotypes of two lung adenocarcinoma cell lines： A549 and GLC-82 with the latter line derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines. Thirteen and twenty-four abnormal chromosomes were identified An A549 and GLC-82 cell lines, respectively. Almost half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines.展开更多
The non-dominated sorting genetic algorithm （NSGA） is improved with the controlled elitism and dynamic crowding distance. A novel multi-objective optimization algorithm is obtained for wind turbine blades. As an exa...The non-dominated sorting genetic algorithm （NSGA） is improved with the controlled elitism and dynamic crowding distance. A novel multi-objective optimization algorithm is obtained for wind turbine blades. As an example, a 5 MW wind turbine blade design is presented by taking the maximum power coefficient and the minimum blade mass as the optimization objectives. The optimal results show that this algorithm has good performance in handling the multi-objective optimization of wind turbines, and it gives a Pareto-optimal solution set rather than the optimum solutions to the conventional multi objective optimization problems. The wind turbine blade optimization method presented in this paper provides a new and general algorithm for the multi-objective optimization of wind turbines.展开更多
The main function of electronic support measure system is to detect threating signals in order to take countermeasures against them. To accomplish this objective, a process of associating each interleaved pulse with i...The main function of electronic support measure system is to detect threating signals in order to take countermeasures against them. To accomplish this objective, a process of associating each interleaved pulse with its emitter must be done. This process is termed sorting or de-interleaving. A novel point symmetry based radar sorting (PSBRS) algorithm is addressed. In order to deal with all kinds of radar signals, the symmetry measure distance is used to cluster pulses instead of the conventional Euclidean distance. The reference points of the symmetrical clusters are initialized by the alternative fuzzy c-means (AFCM) algorithm to ameliorate the effects of noise and the false sorting. Besides, the density filtering (DF) algorithm is proposed to discard the noise pulses or clutter. The performance of the algorithm is evaluated under the effects of noise and missing pulses. It has been observed that the PSBRS algorithm can cope with a large number of noise pulses and it is completely independent of missing pulses. Finally, PSBRS is compared with some benchmark algorithms, and the simulation results reveal the feasibility and efficiency of the algorithm.展开更多
基金National Natural Science Foundation of China,Grant/Award Number:32302826 and 32372961Jilin Provincial Special Project for Health Research Talents,Grant/Award Number:2020SCZ40China Postdoctoral Science Foundation,Grant/Award Number:2023M740623。
文摘Background:Mastitis seriously affects the mammary health of humans and animals.Studies have found that inflammation and oxidative stress play key roles in the occur-rence and development of mastitis.Therefore,in-depth research on related molecular mechanisms is of great significance.Methods:Postpartum mice were anesthetized with pentobarbital and administered lipopolysaccharide to develop the mouse mastitis model.Proteomic analysis was per-formed to compare protein expression in mitochondria-associated endoplasmic retic-ulum membranes(MAM)from two mouse mammary gland groups.Western blot was used to detect the expression of MAM-related proteins in mitochondria.AlphaFold3 was used to predict the molecular structures of phosphofurin acidic cluster sorting protein 2(PACS2)and mitofusin 2(MFN2)and their interaction levels.The MFN2-PACS2 interaction was investigated using co-immunoprecipitation and small interfer-ing RNA.Results:The results showed that the inflammation level in the mammary gland tissue of mice with mastitis significantly increased,the total antioxidant capacity decreased,and the expression of MAM-related proteins MFN2 and PACS2 was significantly downregulated.In cell experiments,overexpression of MFN2 can inhibit inflamma-tion and oxidative stress responses,and promote the interaction between MFN2 and PACS2 to affect the formation of MAMs.Conclusion:In summary,this study suggests that mastitis can alter the expression of MAM-related proteins in mouse breast tissue.The interaction between MFN2 and PACS2 regulates the formation of MAMs.Overexpression of MFN2 can promote the formation of MAMs and inhibit inflammation and oxidative stress response in mam-mary epithelial cells.Our results provided a new theoretical basis and potential thera-peutic targets for the prevention and treatment of mastitis.
基金Supported by Shanghai Science and Technology Development Program, No. 03DZ14024the National High Technology 863 Programs, No. 2002BAC11A11National Development Program (973) for Key Basic Research of China, No. 2001CB510205
文摘AIM： To develop a method to isolate liver stem cells fast and efficiently. METHODS： Fetal mouse liver cells were characterized by cell surface antigens （c-Kit and CD45/TER119） using flow cytometry. The candidate liver stem cells were sorted by using immuno-magnetic microbeads and identified by -lone-forming culture, RT-PCR and immunofluorescence says. RESULTS： The c-Kit-（CD45/TER119）- cell population with 97.9% of purity were purified by immuno-magnetic microbeads at one time. The yield of this separation was about 6% of the total sorting cells and the cell viability was above 98%. When cultured in vitro these cells had high clone-forming and self-renewing ability and expressed markers of hepatocytes and bile duct cells. Functionally mature hepatocytes were observed after 21 d of culture. CONCLUSION： This method offers an excellent tool for the enrichment of liver stem cells with high purity and viability, which could be used for further studies. It is fast, efficient, simple and not expensive.
基金funded by the National Natural Science Foundation of China(31771863)Science and Technology Major Project of the Fujian Province of China(2015NZ0002-2)+3 种基金Special Fund for Scientific and Technological Innovation of the Fujian Agriculture and Forestry University(KFA17168A)Doctoral Students of Fujian Agriculture and Forestry University Going Abroad to Cooperative Research(324-112110082)Key Laboratory of Conservation and Utilization of Subtropical Agricultural Biological Resources(SKLCUSA-a201912)supported by ERDF Project“Plants as a tool for sustainable global development”(CZ.02.1.01/0.0/0.0/16_019/0000827)。
文摘Erianthus arundinaceus is a wild relative of sugarcane(Saccharum officinarum L.)with many desirable agronomic traits for sugarcane genetic improvement.However,limited knowledge of the complex genome of hexaploid E.arundinaceus has impeded the development of required molecular tools.Dissecting complex genomes into single chromosomes can simplify analyses.The flow-cytometric sorting of a single chromosome of E.arundinaceus in a Saccharum-Erianthus introgression line is reported.A novel approach called genomic in situ hybridization in suspension was used to discriminate the alien chromosome from sugarcane chromosomes at the same size.A total of 218,000 E.arundinaceus chromosome 1(EaC1)were sorted to>97%purity and amplified DNA was sequenced using Illumina and Pac Bio technologies.The resulting assembly had a 70.93 Mb contig sequence with an N50 of 19.62 kb.A total of 56.69 Mb repeat sequences were predicted,accounting for 79.1%of the chromosome and 2646 genes having a total length of 1.84 Mb that represented 2.59%of the chromosome.Of these genes,1877(70.9%)genes were functionally annotated.The phylogenetic relationship of E.arundinaceus with other species using the chromosome1 sequence revealed that E.arundinaceus was distantly related to Oryza sativa and Zea mays,followed by Sorghum bicolor,and was closely related to S.spontaneum and Saccharum spp.hybrids.This study provides the first insights into the characteristics of EaC1,and the results will provide tools to support molecular improvement and alien introgression breeding of sugarcane.
基金supported by the National 985 Project "linguistic science technology and the construction of interdisciplinary innovation platform in current society",No.985yk002the National 985 Project "cognitive and neural information science platform",No.904273258
文摘PC12 cell injury was induced using 20 μM amyloid β-protein 25-35 to establish a model of Alzheimer＇s disease. The cells were then treated with 5, 10, and 25 μM Schisandrin B. Methylthiazolyldiphenyl-tetrazolium bromide assays and Hoechst 33342 staining results showed that with increasing Schisandrin B concentration, the survival rate of PC12 cells injured by amyloid β-protein 25-35 gradually increased and the rate of apoptosis gradually decreased. Reverse transcription-PCR, immunocytochemical staining and western blot results showed that with increasing Schisandrin B concentration, the mRNA and protein expression of vacuolar protein sorting 35 and amyloid precursor protein were gradually decreased. Vacuolar protein sorting 35 and amyloid precursor protein showed a consistent trend for change. These findings suggest that 5, 10, and 25 μM Schisandrin B antagonizes the cellular injury induced by amyloid β-protein 25-35 in a dose-dependent manner. This may be caused by decreasing the expression of vacuolar protein sorting 35 and amyloid precursor protein.
基金supported by the Key Science & Technology Project of Gansu Province (22ZD6NA007)the National Key Research and Development Program of China (2021YFD2200202)Computing support was provided by the Supercomputing Center of Lanzhou University
文摘The transition of traits between genetically related lineages is a fascinating topic that provides clues to understanding the drivers of speciation and diversification.Much can be learned about this process from phylogeny-based trait evolution.However,such inference is often plagued by genome-wide gene-tree discordance(GTD),mostly due to incomplete lineage sorting(ILS)and/or introgressive hybridization,especially when the genes underlying the traits appear discordant.Here,by collecting transcriptomes,whole chloroplast genomes(cpDNA),and population genetic datasets,we used the coalescent model to turn GTD into a source of information for ILS and employed hemiplasy to explain specific cases of apparent“phylogenetic discordance”between different morphological traits and probable species phylogeny in the Allium subg.Cyathophora.Both concatenation and coalescence methods consistently showed the same phylogenetic topology for species tree inference based on single-copy genes(SCGs),as supported by the KS distribution.However,GTD was high across the genomes of subg.Cyathophora:~27%e38.9%of the SCG trees were in conflict with the species tree.Plasmid and nuclear incongruence was also present.Our coalescent simulations indicated that such GTD was mainly a product of ILS.Our hemiplasy risk factor calculations supported that random fixation of ancient polymorphisms in different populations during successive speciation events along the subg.Cyathophora phylogeny may have caused the character transition,as well as the anomalous cpDNA tree.Our study exemplifies how phylogenetic noise can be transformed into evolutionary information for understanding character state transitions along species phylogenies.
文摘AIM TO detect significant clusters of co-expressed genes associated with tumorigenesis that might help to predict stomach adenocarcinoma （SA） prognosis.METHODS The Cancer Genome Atlas database was used to obtain RNA sequences as well as complete clinical data of SA and adjacent normal tissues from patients. Weighted gene co-expression network analysis （WGCNA） was used to investigate the meaningful module along with hub genes. Expression of hub genes was analyzed in 362 paraffin-embedded SA biopsy tissues by immunohistochemical staining. Patients were classified into two groups （according to expression of hub genes）： Weak expression and over-expression groups. Correlation of biomarkers with clinicopathological factors indicated patient survival.RESULTS Whole genome expression level screening identified 6,231 differentially expressed genes. Twenty-four co- expressed gene modules were identified using WGCNA. Pearson＇s correlation analysis showed that the tan module was the most relevant to tumor stage （r = 0.24, P = 7 × 10 -6）. In addition, we detected sorting nexin （SNX）10 as the hub gene of the tan module. SNX10 expression was linked to T category （P = 0.042, x2= 8.708）, N category （P = 0.000, x2= 18.778）, TNM stage （P = 0.001, x2 = 16.744） as well as tumor differentiation （P = 0.000,x2= 251.930）. Patients with high SNX10 expression tended to have longer diseasefree survival （DFS; 44.97 mo vs 33.85 mo, P = 0.000） as well as overall survival （OS; 49.95 vs 40.84 mo, P = 0.000） in univariate analysis. Multivariate analysis showed that dismal prognosis could be precisely predicted clinicopathologically using SNX10 [DFS： P = 0.014, hazard ratio （HR） = 0.698, 95% confidence interval （CI）： 0.524-0.930, OS： P = 0.017, HR = 0.704, 95%CI： 0.528-0.940].CONCLUSION This study provides a new technique for screening prognostic biomarkers of SA. Weak expression of SNX10 is linked to poor prognosis, and is a suitable prognostic biomarker of SA.
基金the High Technology Research and Development Programme of China
文摘Using a high-gradient magnetic cell sorting (MACS) system, CD34^+ cells were isolated from human bone marrow, cord blood, peripheral blood, and cultured in CFU system. The results showed that the excellent recovery (75%) and highest purity (95-99%) were acquired by using the MACS separation system, and the most CFCs were present in CD34^+ population, but not in CD34 fraction by clonogenic assays. The isolation of pure hematopoietic stem cells and progenitor cells is of experimental and clinical importance.
文摘Heterogeneity of biological samples is usually considered a major obstacle for three-dimensional (3D) structure determination of macromolecular complexes. Heterogeneity may occur at the level of composition or conformational variability of complexes and affects most 3D structure determination methods that rely on signal averaging. Here, an approach is described that allows sorting structural states based on a 3D statistical approach, the 3D sampling and classification (3D-SC) of 3D structures derived from single particles imaged by cryo electron microscopy (cryo-EM). The method is based on jackknifing & bootstrapping of 3D sub-ensembles and 3D multivariate statistical analysis followed by 3D classification. The robustness of the statistical sorting procedure is corroborated using model data from an RNA polymerase structure and experimental data from a ribosome complex. It allows resolving multiple states within heterogeneous complexes that thus become amendable for a structural analysis despite of their highly flexible nature. The method has important implications for high-resolution structural studies and allows describing structure ensembles to provide insights into the dynamics of multi-component macromolecular assemblies.
文摘Several parallel sorting techniques on different architectures have been studied for many years. Due to the need for faster systems in today＇s world, parallelism can be used to accelerate applications. Nowadays, parallel operations are used to solve computer problems such as sort and search, which result in a reasonable speed. Sorting is one of the most important operations in computing world. The authors always try to find the best in different areas which the premier is speedup. In this paper, the authors issued a sort with O（logn） time complexity on PRAM EREW （Parallel Random Access Machine Exclusive Read Exclusive Write）. The algorithm is designed in a manner that keeps the tradeoff between the number of processor elements in the architecture and execution time. The simulation of the algorithm proves the theoretical analysis of the algorithm. The results of this research can be utilized in developing faster embedded systems. Sorting on Centralized Diamond （SOCD） algorithm is issued on the novel Centralized Diamond architecture which takes the advantages of Single Instruction Multiple Data （SIMD） architecture. This architecture and the sort on it are intuitive and optimal.
文摘Proteolytic processing of the transmembrane amyloid precursor protein (APP) to aggregation-prone amyloid-β (Aβ) peptide underlies the development of Alzheimer’s disease.
文摘The somatotopic representation of specific body parts is a well-established spatial organizational principle in the primary somatosensory and motor cortices.
基金supported by the National Natural Science Foundation(82130101)the Major Scientific and Technological Projects of Guangdong Province(2023B1111050008)the Post Scientist Fund awarded by the Chinese Academy of Traditional Chinese Medicine(ZZ13035-02)to Shuwen Liu.Regional Joint Foundation of Basic and Applied Basic Research in Guangdong Province(2023A1515111165)to Chan Yang.
文摘The ongoing coronavirus epidemic,including the novel coronavirus(SARS-CoV-2),continues to pose a significant threat to global public health.Host targets address multiple stages of the viral life cycle and provide diverse opportunities for therapeutic interventions.This study identified sorting nexin 10(SNX10)as a facilitator of replication of human coronavirus OC43(HCoV-OC43),underscoring its potential as a novel antiviral target.The knockout of SNX10 significantly suppressed HCoV-OC43 replication both in vivo and in vitro.Immunoprecipitation-mass spectrometry(IP-MS)analysis identified the adaptor protein complex 2 subunitμ1(AP2M1)as a direct interactor of SNX10.Specifically,SNX10 facilitates phosphorylation of the AP2M1,thereby enhancing clathrin-mediated viral endocytosis.Furthermore,subsequent binding and internalization assays revealed that SNX10 knockout significantly inhibits viral entry into host cells.Conversely,the reconstitution of SNX10 fully restored viral entry,thereby confirming the critical and indispensable role of SNX10 in pathogen internalization.Simultaneously,SNX10 was identified as a key factor that promotes endosomal acidification by modulating pH levels,which in turn facilitated the release of the viral genome.Notably,the ablation of SNX10 was found to trigger autophagy activation during infection,thereby maintaining intracellular homeostasis.Additionally,it exerted autonomous antiviral effects through lysosomal degradation pathways.Collectively,these findings demonstrate SNX10 serves as a pivotal regulator of the viral life cycle and underscore its therapeutic potential as a multi-faceted antiviral candidate target capable of simultaneously inhibiting viral internalization,viral genomic release,and hostpathogen equilibrium.
文摘This paper introduces an intelligent garbage-handling trolley model based on an STM32 single chip microcomputer as the control core.The device is driven by four independent motors to achieve automatic tracking,automatic obstacle avoidance,and fixed-point docking.Using external execution structure to realize the car without the use of a mechanical arm,complete garbage collection,storage,and uninstall function.On this basis,the type of garbage is marked by color,and the color recognition sensor is applied to realize the type recognition after garbage collection and put into the corresponding unloading point,to realize its intelligent classification function.It can automatically complete the established task autonomously.
基金supported by Science and Technology Project of State Grid Corporation Headquarters under Grant 5108-202218280A-2-170-XG(Development and Application of Power Time-Sensitive Network Switching Chip。
文摘The progress of modern industry has given rise to great requirements for network transmission latency and reliability in domains such as smart grid and intelligent driving.To address these challenges,the concept of Time-sensitive networking(TSN)is proposed by IEEE 802.1TSN working group.In order to achieve low latency,Cyclic queuing and forwarding(CQF)mechanism is introduced to schedule Timetriggered(TT)flows.In this paper,we construct a TSN model based on CQF and formulate the flow scheduling problem as an optimization problem aimed at maximizing the success rate of flow scheduling.The problem is tackled by a novel algorithm that makes full use of the characteristics and the relationship between the flows.Firstly,by K-means algorithm,the flows are initially partitioned into subsets based on their correlations.Subsequently,the flows within each subset are sorted by a new special criteria extracted from multiple features of flow.Finally,a flow offset selecting method based on load balance is used for resource mapping,so as to complete the process of flow scheduling.Experimental results demonstrate that the proposed algorithm exhibits significant advantages in terms of scheduling success rate and time efficiency.
基金supported by the Natural Science Foundation of Shaanxi Province(2023-JC-QN-0728)the China Postdoctoral Science Foundation(2021M693942)。
文摘Manned aerial vehicle-unmanned aerial vehicle(MAV-UAV)combat organization is a MAV-UAV combat collective formed from the perspective of organization design theory and methodology,and the generation of force formation plan is a key step in the organizational planning.Based on the description of the problem and the definition of organizational elements,the matching model of platform-target attack wave is constructed to minimize the redundancy of command and decision-making capability,resource capability and the number of platforms used.Based on the non-dominated sorting genetic algorithmⅢ(NSGA-Ⅲ)framework,which includes encoding/decoding method and constraint handling method,the generation model of organizational force formation plan is solved,and the effectiveness and superiority of the algorithm are verified by simulation experiments.
基金supported partly by grants from the Ministry of Science and Technology of China(2005DKA21502)the Joint Foundation of Science and Technology Bureau of Yunnan Province and Kunming Medical University(2007C0024R)
文摘Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome painting was used to characterize karyotypes of two lung adenocarcinoma cell lines： A549 and GLC-82 with the latter line derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines. Thirteen and twenty-four abnormal chromosomes were identified An A549 and GLC-82 cell lines, respectively. Almost half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines.
基金Project supported by the National Basic Research Program of China (973 Program) (No. 2007CB714600)
文摘The non-dominated sorting genetic algorithm （NSGA） is improved with the controlled elitism and dynamic crowding distance. A novel multi-objective optimization algorithm is obtained for wind turbine blades. As an example, a 5 MW wind turbine blade design is presented by taking the maximum power coefficient and the minimum blade mass as the optimization objectives. The optimal results show that this algorithm has good performance in handling the multi-objective optimization of wind turbines, and it gives a Pareto-optimal solution set rather than the optimum solutions to the conventional multi objective optimization problems. The wind turbine blade optimization method presented in this paper provides a new and general algorithm for the multi-objective optimization of wind turbines.
基金supported by the National Natural Science Foundation of China(61172116)
文摘The main function of electronic support measure system is to detect threating signals in order to take countermeasures against them. To accomplish this objective, a process of associating each interleaved pulse with its emitter must be done. This process is termed sorting or de-interleaving. A novel point symmetry based radar sorting (PSBRS) algorithm is addressed. In order to deal with all kinds of radar signals, the symmetry measure distance is used to cluster pulses instead of the conventional Euclidean distance. The reference points of the symmetrical clusters are initialized by the alternative fuzzy c-means (AFCM) algorithm to ameliorate the effects of noise and the false sorting. Besides, the density filtering (DF) algorithm is proposed to discard the noise pulses or clutter. The performance of the algorithm is evaluated under the effects of noise and missing pulses. It has been observed that the PSBRS algorithm can cope with a large number of noise pulses and it is completely independent of missing pulses. Finally, PSBRS is compared with some benchmark algorithms, and the simulation results reveal the feasibility and efficiency of the algorithm.
