Rapid,precise,and tunable regulation of protein abundance would be significantly useful in a variety of biotechnologies and biomedical applications.Here,we describe a system that allows tunable and rapid drug control ...Rapid,precise,and tunable regulation of protein abundance would be significantly useful in a variety of biotechnologies and biomedical applications.Here,we describe a system that allows tunable and rapid drug control of gene expression for either gene activation or inactivation in mammalian cells.We construct the system by coupling Tet-on 3 G and small molecule-assisted shutoff systems,which can respectively induce transcriptional activation and protein degradation in the presence of corresponding small molecules.This dual-input drug inducer regulation system facilitates a bidirectional control of gene expression.The gene of interest can be precisely controlled by dual small molecules in a broad dynamic range of expression from overexpression to complete silence,allowing gene function study in a comprehensive expression profile.Our results reveal that the bidirectional control system enables sensitive dosage-and time-dependent regulation for either turn-on or shutoff of gene expression.We also apply this system for inducible genome editing and gene activation mediated by clustered regularly interspaced short palindromic repeats.The system provides an integrated platform for studying multiple biological processes by manipulating gene expression in a more flexible way.展开更多
Two-line super hybrid rice （Oryza sativa L.） Guiliangyou 2 was taken as the experimental variety, the growth and physiological characteristics of rice under conventional tillage and smashing ridge tillage were compa...Two-line super hybrid rice （Oryza sativa L.） Guiliangyou 2 was taken as the experimental variety, the growth and physiological characteristics of rice under conventional tillage and smashing ridge tillage were compared based on field re- search. The results showed that smashing ridge tillage was beneficial to the tillering growth of rice plants in the the middle and later periods of tillering; under the tillage mode, the white root was more, the vigour was strong during the whole growing period, the SPAD was extremely significantly higher than that of conventional tillage, the net photosynthetic rate of the leaves was high and held green long, which were beneficial to the production and accumulation of photoassimilates; during mature pe- riod, the dry matter accumulation of the overground part under smashing ridge tillage was significantly higher than that of conventional tillage, and the number of productive ears under smashing ridge tillage was more than that of conventional tillage by 27.6×10^4/hm2, thus, the yield-increasing effect was significant and in- creased by 20.36%.展开更多
基金supported by the National Natural Science Foundation of China(81800555,81701580,and 31972926)the National Key R&D Program of China(2018YFC1106400)+1 种基金the Science and Technology Planning Project of Guangdong Province(2015B020229002)the Natural Science Foundation of Guangdong Province(2014A030312013 and 2018A030313128)
文摘Rapid,precise,and tunable regulation of protein abundance would be significantly useful in a variety of biotechnologies and biomedical applications.Here,we describe a system that allows tunable and rapid drug control of gene expression for either gene activation or inactivation in mammalian cells.We construct the system by coupling Tet-on 3 G and small molecule-assisted shutoff systems,which can respectively induce transcriptional activation and protein degradation in the presence of corresponding small molecules.This dual-input drug inducer regulation system facilitates a bidirectional control of gene expression.The gene of interest can be precisely controlled by dual small molecules in a broad dynamic range of expression from overexpression to complete silence,allowing gene function study in a comprehensive expression profile.Our results reveal that the bidirectional control system enables sensitive dosage-and time-dependent regulation for either turn-on or shutoff of gene expression.We also apply this system for inducible genome editing and gene activation mediated by clustered regularly interspaced short palindromic repeats.The system provides an integrated platform for studying multiple biological processes by manipulating gene expression in a more flexible way.
基金Supported by the Special Project of Basic Scientific Research of Guangxi Academy of Agricultural Sciences(Gui Nong Ke 2013YM50)the Special Project of Basic Scientific Research of Guangxi Academy of Agricultural Sciences(Gui Nong Ke 2013YM43)the Planned Project of Guangxi Scientific Research and Technological Development(Gui Ke Neng 1346007-9)~~
文摘Two-line super hybrid rice （Oryza sativa L.） Guiliangyou 2 was taken as the experimental variety, the growth and physiological characteristics of rice under conventional tillage and smashing ridge tillage were compared based on field re- search. The results showed that smashing ridge tillage was beneficial to the tillering growth of rice plants in the the middle and later periods of tillering; under the tillage mode, the white root was more, the vigour was strong during the whole growing period, the SPAD was extremely significantly higher than that of conventional tillage, the net photosynthetic rate of the leaves was high and held green long, which were beneficial to the production and accumulation of photoassimilates; during mature pe- riod, the dry matter accumulation of the overground part under smashing ridge tillage was significantly higher than that of conventional tillage, and the number of productive ears under smashing ridge tillage was more than that of conventional tillage by 27.6×10^4/hm2, thus, the yield-increasing effect was significant and in- creased by 20.36%.
