High shear wet granulation(HSWG) is one of the most poorly understood processes with known difficulties in optimization and scale up. The purpose of the current study is to develop a DEM model which can be applied und...High shear wet granulation(HSWG) is one of the most poorly understood processes with known difficulties in optimization and scale up. The purpose of the current study is to develop a DEM model which can be applied under dynamic process conditions with high predictive capacity to improve process insight. The DEM model is used to predict agglomeration as a function of impeller speed and liquid addition rate in a high shear wet granulator. The DEM model tracks dynamic formation and breakage of liquid bridges between particles as liquid binder in the system is added, and corrects for the change in material properties as a function of the binder content.展开更多
The microwave absorbents of Fe and C nanoparticles as magnetic loss and dielectric loss material respectively were composited with the polyvinyl alcohol(PVA)as binder by spray granulation method,The electromagnetic pa...The microwave absorbents of Fe and C nanoparticles as magnetic loss and dielectric loss material respectively were composited with the polyvinyl alcohol(PVA)as binder by spray granulation method,The electromagnetic parameters of Fe and C composite particles were analyzed by vector network.The complex permittivity and magnetic permeability of Fe and C composite particles matched well with increasing C nanoparticle content,and then the microwave loss property was improved.A minimum reflection loss(RL)of-42.7 dB at 3.68 GHz for a composite with 4.6 mm in thickness can be obtained when the content ratio of the C nanoparticles,the modified Fe nanoparticles and the PVA is 21:49:30(Sample 3).展开更多
In order to control the accumulation of SiC ceramic particles on the wall of the rotating chamber in the frame of a dry granulation process,the effect of the wall reverse speed on the mixing process is investigated.In...In order to control the accumulation of SiC ceramic particles on the wall of the rotating chamber in the frame of a dry granulation process,the effect of the wall reverse speed on the mixing process is investigated.In particular,an Euler-Euler two-phase flow model is used to analyze the dynamics of both SiC particles and air.The numerical results show that by setting a certain reverse rotating speed of the rotating chamber,the accumulation of SiC particles on the wall can be improved,i.e.,their direction of motion in proximity to the wall can be changed and particles can be forced to re-join the granulation process.Experimental tests conducted to verify the reliability of the numerical findings,demonstrate that when the reverse rotating speed of the rotating chamber is 4 r/min,the sphericity of SiC particles in the rotating chamber is the highest and the fluidity is the best possible one.展开更多
In order to reveal the intrinsic fluid-dynamic mechanisms of a pressure-swirl nozzle used for Si3N4 dry granulation,and effectively predict its external spray characteristics,the dynamics of air-atomized liquid two-ph...In order to reveal the intrinsic fluid-dynamic mechanisms of a pressure-swirl nozzle used for Si3N4 dry granulation,and effectively predict its external spray characteristics,the dynamics of air-atomized liquid two-phase flow is analyzed using a VOF(Volume of Fraction)method together with the modified realizable k-εturbulence model.The influence of nozzle orifice shape on velocity distribution,pressure distribution is studied.The results show that the pressure difference in a convergent conical nozzle is the largest with a hollow air core being formed in the nozzle.The corresponding velocity of atomized liquid at nozzle orifice is the largest.Using a self-designed atomization experiment platform,the velocity and pressure of atomized liquid and the spray cone angle are measured for three nozzles with different orifice shapes.The micro-morphology of Si3N4 particles is also determined.These data confirm the correctness of numerical simulation.Considering atomization performance of the nozzle,the contraction conical nozzle is more suitable for the atomization of Si3N4 in practical production based on the dry granulation approach.展开更多
A novel method isolated microorganisms in soil granule was built. The key steps included： repeated elutriation of soil by sterilized water, inoculation on the plates with the elutriated sediments, incubation of the p...A novel method isolated microorganisms in soil granule was built. The key steps included： repeated elutriation of soil by sterilized water, inoculation on the plates with the elutriated sediments, incubation of the plates and isolation of the actinomycetes by using selected culture medium. We formulated that most microflora included the dominant actinomycetes in the soil were carried away with the sterilized water in the elutriation procedure, some rare actinomycetes and few other microflora included bacteria were remained in the elutriated sediments, the other microflora were excluded to grew into colonies on the plates by using selective culture medium for actinomycetes in the elutriated sediments. Results showed the supposition. Non-streptomycete actinomycetes were isolated both from black soil samples from Chinese northeast area and compost samples from Chinese central area. Soil fungi in granule were isolated by using the selective conditions to favor fungi. The results showed that the method was effective展开更多
This paper deals with a comparative research between two processes of granulation, namely mini-pelletized sintering （MPS） and hybrid pelletized sintering （HPS）, focusing on aspects such as the balling effect, prod...This paper deals with a comparative research between two processes of granulation, namely mini-pelletized sintering （MPS） and hybrid pelletized sintering （HPS）, focusing on aspects such as the balling effect, production, quality, and mineralography and metallurgical performance of sinter. The results indicate that both methods can result in a satisfying capability in balling effect and metallurgical performance of sinter, qualified to meet the plant production requirements, but when the granulation time and granulating moisture are set to around 6 minutes and 7.0% respectively, MPS is better than HPS.展开更多
The Co content dependence of crystal structure and specific magnetization of Fe1-xCox-SiO2granular solid prepared by the sol-gel method have been studied. It is found that the crystal structure, Iattice parameter and ...The Co content dependence of crystal structure and specific magnetization of Fe1-xCox-SiO2granular solid prepared by the sol-gel method have been studied. It is found that the crystal structure, Iattice parameter and specific magnetization of the FeCo alloy particles depend on the Co content.展开更多
In order to reveal the force transmission features of the granules in the solid granule medium forming(SGMF) technology,the frictional characteristics of the non-metallic granule medium(NGM) under high pressure were i...In order to reveal the force transmission features of the granules in the solid granule medium forming(SGMF) technology,the frictional characteristics of the non-metallic granule medium(NGM) under high pressure were investigated by tests and simulations.And the relevant changing curves of the internal friction coefficient of the granular system under different normal pressures were obtained by self-designed shear test.By the granule volume compression test,the accurate discrete element simulation parameters were obtained,based on this,the discrete element method(DEM) was adopted to reveal the evolution law of the NGM granules movement in the sample shear process from the microscopic view.Based on the DEM,the influence of granule diameter,surface friction coefficient,normal pressure and shear velocity on the internal friction coefficient of the granular system were studied.And the parameters were conducted to be dimensionless by introducing the inertia coefficient.Finally,the expression showing power-law relationship of inertia coefficient,surface friction coefficient and internal friction coefficient is obtained.展开更多
Objective To facilitate the quality evaluation suitable for the unique characteristics of Chinese materia medica(CMM)by developing and implementing a novel approach known as the matching frequency statistical moment(M...Objective To facilitate the quality evaluation suitable for the unique characteristics of Chinese materia medica(CMM)by developing and implementing a novel approach known as the matching frequency statistical moment(MFSM)method.Methods This study established the MFSM method.To demonstrate its effectiveness,we applied this novel approach to analyze Danxi Granules(丹膝颗粒,DXG)and its constituent herbal materials.To begin with,the ultra-performance liquid chromatography(UPLC)was applied to obtain the chromatographic fingerprints of DXG and its constituent herbal materi-als.Next,the MFSM was leveraged to compress and integrate them into a new fingerprint with fewer analytical units.Then,we characterized the properties and variability of both the original and integrated fingerprints by calculating total quantum statistical moment(TQSM)parameters,information entropy and information amount,along with their relative standard deviation(RSD).Finally,we compared the TQSM parameters,information entropy and infor-mation amount,and their RSD between the traditional and novel fingerprints to validate the new analytical method.Results The chromatographic peaks of DXG and its 12 raw herbal materials were divided and integrated into peak families by the MFSM method.Before integration,the ranges of the peak number,three TQSM parameters,information entropy and information amount for each peak or peak family of UPLC fingerprints of DXG and its 12 raw herbal materials were 95.07−209.73,9390−183064μv·s,5.928−21.33 min,22.62−106.69 min^(2),4.230−6.539,and 50530−974186μv·s,respectively.After integration,the ranges of these parameters were 10.00−88.00,9390−183064μv·s,5.951−22.02 min,22.27−104.73 min^(2),2.223−5.277,and 38159−807200μv·s,respectively.Correspondingly,the RSD of all the aforementioned pa-rameters before integration were 2.12%−9.15%,6.04%−49.78%,1.15%−23.10%,3.97%−25.79%,1.49%−19.86%,and 6.64%−51.20%,respectively.However,after integration,they changed to 0.00%,6.04%−49.87%,1.73%−23.02%,3.84%−26.85%,1.17%−16.54%,and 6.40%−48.59%,respectively.The results demonstrated that in the newly integrated fingerprint,the analytical units of constituent herbal materials,information entropy and information amount were significantly reduced(P<0.05),while the TQSM parameters remained unchanged(P>0.05).Additionally,the RSD of the TQSM parameters,information entropy,and information amount didn’t show significant difference before and after integration(P>0.05),but the RSD of the number and area of the integrated analytical units significantly decreased(P<0.05).Conclusion The MFSM method could reduce the analytical units of constituent herbal mate-rials while maintain the properties and variability from their original fingerprint.Thus,it could serve as a feasible and reliable tool to reduce difficulties in analyzing multi-compo-nents within CMMs and facilitating the evaluation of their quality.展开更多
[Objectives]To test the microbial limit of Zhideke granules.[Methods]According to the relevant regulations on the testing of granules in Chinese Pharmacopoeia in 2015,the applicability of the methods for counting the ...[Objectives]To test the microbial limit of Zhideke granules.[Methods]According to the relevant regulations on the testing of granules in Chinese Pharmacopoeia in 2015,the applicability of the methods for counting the total number of aerobic bacteria,molds and yeasts and objectionable organisms in Zhideke granules was studied.[Results]The total number of aerobic bacteria in Zhideke granules was determined by dilution plate method(1∶20,1 mL per dish),the total number of molds and yeasts was determined by conventional plate method(1∶10,1 mL per dish),and E.coli was detected by conventional method of TTB(1∶10 test solution,10-100 mL TSB).[Conclusions]The method was suitable for the microbial limit test of Zhideke granules.展开更多
A high-performance liquid chromatographic method was applied to the determination of stachydrine hydrochloride concentration in TJF granule (Chinese name: Tiao-Jing-Fang), using a mobile phase of methanol-acetonitrile...A high-performance liquid chromatographic method was applied to the determination of stachydrine hydrochloride concentration in TJF granule (Chinese name: Tiao-Jing-Fang), using a mobile phase of methanol-acetonitrile (50:50, v/v) by the Agilent Kromasi NH2 column (250 mm × 4.6 mm, 5 μm, S/N: 22N25110). Detection wavelength was 201 nm. The result revealed good linearity of stachydrine hydrochloride and was obtained within the range of 0.20 - 1.98 μg/mL (R = 0.9995). The average recovery was 97.01%;the relative standard deviation (RSD) was 0.19%. To the best of our knowledge, this is the first report dedicated to the determination of stachydrine hydrochloride by the evaporative light scattering detector-high-performance liquid chromatographic (ELSD-HPLC) method.展开更多
[Objectives]The research aimed to study application of digital technique in color test of Compound Lonicera Granules.[Methods]A method of color identification of Compound Lonicera Granules based on digital technology ...[Objectives]The research aimed to study application of digital technique in color test of Compound Lonicera Granules.[Methods]A method of color identification of Compound Lonicera Granules based on digital technology was proposed,and the"four-point collection method of color data"was summarized through experiments.[Results]The method was used to test color of 168 batches of Compound Lonicera Granules,and it was found that the method had the advantages of fast speed,high accuracy and good repeatability.[Conclusions]The method was used to test color of 168 batches of Compound Lonicera Granules,and it was found that the method had the advantages of fast speed,high accuracy and good repeatability.展开更多
[Objectives] This study was conducted to establish microbial limit test methods for traditional Chinese medicine preparations Yunpi Granules and Bupi Qiangli Paste. [Methods] According to General Rules of Part IV of C...[Objectives] This study was conducted to establish microbial limit test methods for traditional Chinese medicine preparations Yunpi Granules and Bupi Qiangli Paste. [Methods] According to General Rules of Part IV of Chinese Pharmacopoeia, applicability tests were conducted on microbial limit test methods for the above two traditional Chinese medicine preparations by the plate method. [Results] The established methods showed recovery values in the range of 0.5-2.0 for both experimental strains, and the control bacteria could be detected in the experimental group, but not in the negative control group. [Conclusions] The microbial limit test methods were reliable for the two traditional Chinese medicine preparations and could be used for quality control.展开更多
Objective: to establish multi-components by single marker (QAMS) and HPlC method for the determination of four substances (R, S) - Epigoitrin, Vitexicarpin, Indirubin and Artemisetin and to provide experimental basis ...Objective: to establish multi-components by single marker (QAMS) and HPlC method for the determination of four substances (R, S) - Epigoitrin, Vitexicarpin, Indirubin and Artemisetin and to provide experimental basis for the quality standard of Compound Yizhihao Granules. Methods: the sample treatment method was improved, and HPLC external standard method was established for the determination of four substances in Compound Yizhihao Granules. The chromatographic column was Wondersil C18 (4.6mm×250mm, 5μm), the flow rate was 1.0mL/min, the check wavelength was 245nm, the column temperature was 30℃, the sample was 20 μL, the mobile phase was acetonitrile-water. The relative correction factors (RCF) of (R, S) - Epigoitrin, Vitexicarpin, Artemisetin were determined with Indirubin as internal reference. The RCF was used to calculate the content of the substance under test, and then the content of the substance under test was determined by the external standard method to verify the feasibility of the multi-components by single marker. Results: the treatment method of the test sample was as follows: solid-liquid ratio was 1:5. Ultrasound was 45 min, trichloromethane extraction, and steam dry concentration with methanol constant volume. The results showed that the good linear ranges of (R, S) - Epigoitrin, Vitexicarpin, Indirubin and Artemisetin were 5.15-103 μ g / mL, 5.005-100.1 μ g / mL, 0.5-10 μ g / mL and 0.5115-10.23 μ g / mL, respectively. The average recoveries were 95.19%, 95.73%, 90.75%, 91.65%. The relative correction factors (RCF) of (R, S) - Epigoitrin, Vitexicarpin, Artemisetin were determined with Indirubin as internal reference was 0.2%, 0.6% and 0.3%. And the relative error of multi-components by single marker and external standard method was less than 1%. Conclusion: the multi-components by single marker method (QAMS) is simple, stable and can be used for the determination of multiple substances in Compound Yizhihao Granules.展开更多
基金financial support by PhR MA Foundation Starter Research Grant in Pharmaceutics
文摘High shear wet granulation(HSWG) is one of the most poorly understood processes with known difficulties in optimization and scale up. The purpose of the current study is to develop a DEM model which can be applied under dynamic process conditions with high predictive capacity to improve process insight. The DEM model is used to predict agglomeration as a function of impeller speed and liquid addition rate in a high shear wet granulator. The DEM model tracks dynamic formation and breakage of liquid bridges between particles as liquid binder in the system is added, and corrects for the change in material properties as a function of the binder content.
基金the support from the National Natural Science Foundation of China(No.51171033)the Fundamental Research Funds for the Central Universities(DUT15LAB05,DUT16LAB03)
文摘The microwave absorbents of Fe and C nanoparticles as magnetic loss and dielectric loss material respectively were composited with the polyvinyl alcohol(PVA)as binder by spray granulation method,The electromagnetic parameters of Fe and C composite particles were analyzed by vector network.The complex permittivity and magnetic permeability of Fe and C composite particles matched well with increasing C nanoparticle content,and then the microwave loss property was improved.A minimum reflection loss(RL)of-42.7 dB at 3.68 GHz for a composite with 4.6 mm in thickness can be obtained when the content ratio of the C nanoparticles,the modified Fe nanoparticles and the PVA is 21:49:30(Sample 3).
基金the National Natural Science Foundation of China(Grant No.51964022).
文摘In order to control the accumulation of SiC ceramic particles on the wall of the rotating chamber in the frame of a dry granulation process,the effect of the wall reverse speed on the mixing process is investigated.In particular,an Euler-Euler two-phase flow model is used to analyze the dynamics of both SiC particles and air.The numerical results show that by setting a certain reverse rotating speed of the rotating chamber,the accumulation of SiC particles on the wall can be improved,i.e.,their direction of motion in proximity to the wall can be changed and particles can be forced to re-join the granulation process.Experimental tests conducted to verify the reliability of the numerical findings,demonstrate that when the reverse rotating speed of the rotating chamber is 4 r/min,the sphericity of SiC particles in the rotating chamber is the highest and the fluidity is the best possible one.
基金the National Natural Science Foundation of China(Grant:51964022).
文摘In order to reveal the intrinsic fluid-dynamic mechanisms of a pressure-swirl nozzle used for Si3N4 dry granulation,and effectively predict its external spray characteristics,the dynamics of air-atomized liquid two-phase flow is analyzed using a VOF(Volume of Fraction)method together with the modified realizable k-εturbulence model.The influence of nozzle orifice shape on velocity distribution,pressure distribution is studied.The results show that the pressure difference in a convergent conical nozzle is the largest with a hollow air core being formed in the nozzle.The corresponding velocity of atomized liquid at nozzle orifice is the largest.Using a self-designed atomization experiment platform,the velocity and pressure of atomized liquid and the spray cone angle are measured for three nozzles with different orifice shapes.The micro-morphology of Si3N4 particles is also determined.These data confirm the correctness of numerical simulation.Considering atomization performance of the nozzle,the contraction conical nozzle is more suitable for the atomization of Si3N4 in practical production based on the dry granulation approach.
基金Supported by the Youth Foundation of College of Resources and Environment of Northeast Agricultural University
文摘A novel method isolated microorganisms in soil granule was built. The key steps included： repeated elutriation of soil by sterilized water, inoculation on the plates with the elutriated sediments, incubation of the plates and isolation of the actinomycetes by using selected culture medium. We formulated that most microflora included the dominant actinomycetes in the soil were carried away with the sterilized water in the elutriation procedure, some rare actinomycetes and few other microflora included bacteria were remained in the elutriated sediments, the other microflora were excluded to grew into colonies on the plates by using selective culture medium for actinomycetes in the elutriated sediments. Results showed the supposition. Non-streptomycete actinomycetes were isolated both from black soil samples from Chinese northeast area and compost samples from Chinese central area. Soil fungi in granule were isolated by using the selective conditions to favor fungi. The results showed that the method was effective
文摘This paper deals with a comparative research between two processes of granulation, namely mini-pelletized sintering （MPS） and hybrid pelletized sintering （HPS）, focusing on aspects such as the balling effect, production, quality, and mineralography and metallurgical performance of sinter. The results indicate that both methods can result in a satisfying capability in balling effect and metallurgical performance of sinter, qualified to meet the plant production requirements, but when the granulation time and granulating moisture are set to around 6 minutes and 7.0% respectively, MPS is better than HPS.
文摘The Co content dependence of crystal structure and specific magnetization of Fe1-xCox-SiO2granular solid prepared by the sol-gel method have been studied. It is found that the crystal structure, Iattice parameter and specific magnetization of the FeCo alloy particles depend on the Co content.
基金Projects(51305385,51305386)supported by the National Natural Science Foundation of ChinaProject(QN20131080)supported by the Science Research Youth Foundation of Hebei Provincial Colleges and Universities,China
文摘In order to reveal the force transmission features of the granules in the solid granule medium forming(SGMF) technology,the frictional characteristics of the non-metallic granule medium(NGM) under high pressure were investigated by tests and simulations.And the relevant changing curves of the internal friction coefficient of the granular system under different normal pressures were obtained by self-designed shear test.By the granule volume compression test,the accurate discrete element simulation parameters were obtained,based on this,the discrete element method(DEM) was adopted to reveal the evolution law of the NGM granules movement in the sample shear process from the microscopic view.Based on the DEM,the influence of granule diameter,surface friction coefficient,normal pressure and shear velocity on the internal friction coefficient of the granular system were studied.And the parameters were conducted to be dimensionless by introducing the inertia coefficient.Finally,the expression showing power-law relationship of inertia coefficient,surface friction coefficient and internal friction coefficient is obtained.
基金Natural Science Foundation of Hunan province(2022JJ30453 and 2024JJ6362)the Key Research and Development Program of Hunan Province(2022SK2014).
文摘Objective To facilitate the quality evaluation suitable for the unique characteristics of Chinese materia medica(CMM)by developing and implementing a novel approach known as the matching frequency statistical moment(MFSM)method.Methods This study established the MFSM method.To demonstrate its effectiveness,we applied this novel approach to analyze Danxi Granules(丹膝颗粒,DXG)and its constituent herbal materials.To begin with,the ultra-performance liquid chromatography(UPLC)was applied to obtain the chromatographic fingerprints of DXG and its constituent herbal materi-als.Next,the MFSM was leveraged to compress and integrate them into a new fingerprint with fewer analytical units.Then,we characterized the properties and variability of both the original and integrated fingerprints by calculating total quantum statistical moment(TQSM)parameters,information entropy and information amount,along with their relative standard deviation(RSD).Finally,we compared the TQSM parameters,information entropy and infor-mation amount,and their RSD between the traditional and novel fingerprints to validate the new analytical method.Results The chromatographic peaks of DXG and its 12 raw herbal materials were divided and integrated into peak families by the MFSM method.Before integration,the ranges of the peak number,three TQSM parameters,information entropy and information amount for each peak or peak family of UPLC fingerprints of DXG and its 12 raw herbal materials were 95.07−209.73,9390−183064μv·s,5.928−21.33 min,22.62−106.69 min^(2),4.230−6.539,and 50530−974186μv·s,respectively.After integration,the ranges of these parameters were 10.00−88.00,9390−183064μv·s,5.951−22.02 min,22.27−104.73 min^(2),2.223−5.277,and 38159−807200μv·s,respectively.Correspondingly,the RSD of all the aforementioned pa-rameters before integration were 2.12%−9.15%,6.04%−49.78%,1.15%−23.10%,3.97%−25.79%,1.49%−19.86%,and 6.64%−51.20%,respectively.However,after integration,they changed to 0.00%,6.04%−49.87%,1.73%−23.02%,3.84%−26.85%,1.17%−16.54%,and 6.40%−48.59%,respectively.The results demonstrated that in the newly integrated fingerprint,the analytical units of constituent herbal materials,information entropy and information amount were significantly reduced(P<0.05),while the TQSM parameters remained unchanged(P>0.05).Additionally,the RSD of the TQSM parameters,information entropy,and information amount didn’t show significant difference before and after integration(P>0.05),but the RSD of the number and area of the integrated analytical units significantly decreased(P<0.05).Conclusion The MFSM method could reduce the analytical units of constituent herbal mate-rials while maintain the properties and variability from their original fingerprint.Thus,it could serve as a feasible and reliable tool to reduce difficulties in analyzing multi-compo-nents within CMMs and facilitating the evaluation of their quality.
基金Guangxi Special Fund Project for Innovation-driven Development(Gui KeAA17202046)Guangxi 1000 Young and Middle-aged College and University Backbone Teachers Cultivation Program[(2019)5]+8 种基金Guangxi Graduate Education Innovation Program(YCSY20190081)Guangxi College Students Innovation and Entrepreneurship Training Program(201910600091)Guangxi First-class Discipline—Traditional Chinese Medicine(Ethnopharmacology)Project[Gui Jiao Ke Yan(2018)12]Zhuang Yao Medicine Synergetic Innovation Center[Gui Jiao Ke Yan(2013)20]Guangxi Key Laboratory of Zhuang Yao Medicine[Gui Ke Ji Zi(2014)32]High-level Innovation Team and Outstanding Scholars Program of Guangxi Colleges and Universities[Gui Jiao Shi Fan(2019)52]The 8 th Batch of Special Expert Project in Guangxi Zhuang Autonomous Region[Study on the Quality Standard of Zhuang Yao Medicine,Gui Ren Cai Tong Zi(2019)13]"Qihuang Project"—High-level Talent Team Training Project of Guangxi University of Chinese Medicine(2018002)Guangxi Science and Technology Base and Talent Project(Gui KeAD20238058).
文摘[Objectives]To test the microbial limit of Zhideke granules.[Methods]According to the relevant regulations on the testing of granules in Chinese Pharmacopoeia in 2015,the applicability of the methods for counting the total number of aerobic bacteria,molds and yeasts and objectionable organisms in Zhideke granules was studied.[Results]The total number of aerobic bacteria in Zhideke granules was determined by dilution plate method(1∶20,1 mL per dish),the total number of molds and yeasts was determined by conventional plate method(1∶10,1 mL per dish),and E.coli was detected by conventional method of TTB(1∶10 test solution,10-100 mL TSB).[Conclusions]The method was suitable for the microbial limit test of Zhideke granules.
文摘A high-performance liquid chromatographic method was applied to the determination of stachydrine hydrochloride concentration in TJF granule (Chinese name: Tiao-Jing-Fang), using a mobile phase of methanol-acetonitrile (50:50, v/v) by the Agilent Kromasi NH2 column (250 mm × 4.6 mm, 5 μm, S/N: 22N25110). Detection wavelength was 201 nm. The result revealed good linearity of stachydrine hydrochloride and was obtained within the range of 0.20 - 1.98 μg/mL (R = 0.9995). The average recovery was 97.01%;the relative standard deviation (RSD) was 0.19%. To the best of our knowledge, this is the first report dedicated to the determination of stachydrine hydrochloride by the evaporative light scattering detector-high-performance liquid chromatographic (ELSD-HPLC) method.
文摘[Objectives]The research aimed to study application of digital technique in color test of Compound Lonicera Granules.[Methods]A method of color identification of Compound Lonicera Granules based on digital technology was proposed,and the"four-point collection method of color data"was summarized through experiments.[Results]The method was used to test color of 168 batches of Compound Lonicera Granules,and it was found that the method had the advantages of fast speed,high accuracy and good repeatability.[Conclusions]The method was used to test color of 168 batches of Compound Lonicera Granules,and it was found that the method had the advantages of fast speed,high accuracy and good repeatability.
基金Supported by Science and Technology Planning Project of Guiyang CityGuizhou Province (ZKHT [2019]-9-4-15)+2 种基金Green Seedling Research Startup Foundation of The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine (GZEYK-Y[2022]29)Research and Development CenterThe Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine(3040-04020001406)。
文摘[Objectives] This study was conducted to establish microbial limit test methods for traditional Chinese medicine preparations Yunpi Granules and Bupi Qiangli Paste. [Methods] According to General Rules of Part IV of Chinese Pharmacopoeia, applicability tests were conducted on microbial limit test methods for the above two traditional Chinese medicine preparations by the plate method. [Results] The established methods showed recovery values in the range of 0.5-2.0 for both experimental strains, and the control bacteria could be detected in the experimental group, but not in the negative control group. [Conclusions] The microbial limit test methods were reliable for the two traditional Chinese medicine preparations and could be used for quality control.
文摘Objective: to establish multi-components by single marker (QAMS) and HPlC method for the determination of four substances (R, S) - Epigoitrin, Vitexicarpin, Indirubin and Artemisetin and to provide experimental basis for the quality standard of Compound Yizhihao Granules. Methods: the sample treatment method was improved, and HPLC external standard method was established for the determination of four substances in Compound Yizhihao Granules. The chromatographic column was Wondersil C18 (4.6mm×250mm, 5μm), the flow rate was 1.0mL/min, the check wavelength was 245nm, the column temperature was 30℃, the sample was 20 μL, the mobile phase was acetonitrile-water. The relative correction factors (RCF) of (R, S) - Epigoitrin, Vitexicarpin, Artemisetin were determined with Indirubin as internal reference. The RCF was used to calculate the content of the substance under test, and then the content of the substance under test was determined by the external standard method to verify the feasibility of the multi-components by single marker. Results: the treatment method of the test sample was as follows: solid-liquid ratio was 1:5. Ultrasound was 45 min, trichloromethane extraction, and steam dry concentration with methanol constant volume. The results showed that the good linear ranges of (R, S) - Epigoitrin, Vitexicarpin, Indirubin and Artemisetin were 5.15-103 μ g / mL, 5.005-100.1 μ g / mL, 0.5-10 μ g / mL and 0.5115-10.23 μ g / mL, respectively. The average recoveries were 95.19%, 95.73%, 90.75%, 91.65%. The relative correction factors (RCF) of (R, S) - Epigoitrin, Vitexicarpin, Artemisetin were determined with Indirubin as internal reference was 0.2%, 0.6% and 0.3%. And the relative error of multi-components by single marker and external standard method was less than 1%. Conclusion: the multi-components by single marker method (QAMS) is simple, stable and can be used for the determination of multiple substances in Compound Yizhihao Granules.
