Mantle cell lymphoma(MCL)is recognized as one of the most genetically heterogeneous diseases,with high instability at the genomic level.MCL is common in males with a male-to-female ratio of about 2:1,and its incidence...Mantle cell lymphoma(MCL)is recognized as one of the most genetically heterogeneous diseases,with high instability at the genomic level.MCL is common in males with a male-to-female ratio of about 2:1,and its incidence accounts for 3%e10%of adult non-Hodgkin lymphoma cases.The evolutionary dynamics of MCL clones at the single-cell level remain largely unclear.Our research suggests that MCL may arise from multiple cells within the abnormal microenvironment of the entire hematopoietic lineage,particularly from initiating cells.These initiating cells predominantly consist of CD19^(-)/IgM^(-) subclones and exhibit a disrupted malignant clonal differentiation of pre-B cells along the tumor immunity evolution tree.展开更多
Multiplexed fluorescence measurement at the singlemolecule level provides unique insight into the molecular composition and interaction within a complex system.1,2 However,fluorescence-based detection is typically res...Multiplexed fluorescence measurement at the singlemolecule level provides unique insight into the molecular composition and interaction within a complex system.1,2 However,fluorescence-based detection is typically restricted to 3-4 colors at a time,due to a low signal-to-noise ratio,high spectral overlap,and the need to maintain chemical compatibility of dyes.Although chemically diverse fluorophores offer a potentially broad spectroscopic palette,further multiplexing is ultimately constrained by the chemical compatibility and varying labeling performance of different fluorophores.展开更多
Background For young patients,the surgical method for lumbar disc herniation remains controversial.The aim of this study was to prospectively determine the short-term clinical outcome after surgery for young patients ...Background For young patients,the surgical method for lumbar disc herniation remains controversial.The aim of this study was to prospectively determine the short-term clinical outcome after surgery for young patients with lumbar disc herniation.Methods In this prospective comparative study between April 2010 and August 2011,a total of 80 patients underwent primary surgery at a single level for lumbar disc herniation.The patients were divided into two groups：decompression alone and decompression with instrumented fusion.An independent examiner clinically evaluated the patients at preoperation and at 1,3,6,and 12 months after surgery.The patients filled out the instruments for back and leg pain using a Visual Analog Scale （VAS）,Oswestry Low Back Pain Disability Questionnaire （ODI）,and Japanese Orthopaedic Association （JOA） scores.The differences between the two groups were analyzed.Results The mean age of all the patients at the time of surgery was 33.7 years.Of the 80 patients,38 patients underwent decompression alone and 42 patients underwent posterior lumbar interbody fusion.Increasing complexity of surgery was associated with a longer surgery time,greater blood loss,and a longer hospital stay after surgery.Both methods of surgery independently improved outcomes compared with baseline status based on VAS,ODI,and JOA scores （P 〈0.05）,and no significant differences were found between the two groups at most of the measuring points in time,although patients with decompression alone had a higher JOA score （P=0.016） and higher JOA recovery rate （P=0.010） at the 3-month follow-up.Conclusions The short-term results of our study showed that both methods of surgery obtained effective clinical outcomes,but decompression alone had some advantages （shorter surgery time,less blood loss,shorter hospital stay,and lower cost） compared with decompression with instrumented fusion.Young patients with decompression alone could achieve great physical function earlier.展开更多
Monitoring subtle changes in ionic current flow through a nanopore could be applied to observe single molecule reaction. Here,we introduced cysteine to substitute for lysine at position 238 constructing a mutant aerol...Monitoring subtle changes in ionic current flow through a nanopore could be applied to observe single molecule reaction. Here,we introduced cysteine to substitute for lysine at position 238 constructing a mutant aerolysin K238 C. It could be regarded as a nanoreactor to efficiently visualize chemical bonds making and breaking. The compound 5,5′-dithiobis-(2-nitrobenzoic acid)(DTNB) was selected as a reactant coming into collisions on the interface of the pore to occur a reversible reaction. Our results showed that the mutant aerolysin could respond to three molecules of DTNB simultaneously and reflect corresponding levels with distinguishable current signals. Therefore, this method constitutes a simple, generic tool for monitoring single molecule reaction, which evokes a guidance for the mutant aerolysin towards the application of tracking other more reactions at single molecule level.展开更多
The transition among multiple charging states of a semiconductor＇s localized intrinsic/impurity defects is considered as phase transitions, and the concept of transition Gibbs free energy level （TGFEL） is proposed....The transition among multiple charging states of a semiconductor＇s localized intrinsic/impurity defects is considered as phase transitions, and the concept of transition Gibbs free energy level （TGFEL） is proposed. Dependence of the cross section of TGFEL on its charge state is discussed. Introduction of TGFEL to replace acti- vation energy has fundamentally important consequences for semiconductor physics and devices. TGFEL involves entropy. What is to be included and not included in the entropy term consistently for all defect levels is an unre- solved open question, related to correct interpretation of various experimental data associated with various defect levels. This work is a first step towards resolving this question.展开更多
基金funded by the Chongqing Medical Scientific Research Project(Joint Project of Chongqing Health Commission and Science and Technology Bureau,China)(No.2023GGXM006,2024ZDXM026,2024MSXM115)the Key Research Project from Science and Technology Department of Sichuan Province,China(No.2019YFS0301)+3 种基金the Chongqing Key Municipal Public Health Specialty Construction Project(China)the Key Research Project from Chongqing Medical and Pharmaceutical Vocational Education Group(China)(No.CQZJ202329)the Incubation Project of the First Affiliated Hospital of Chongqing Medical and Pharmaceutical College(China)(No.2022-2023ZD04,2022-2023ZD03,2022-2023MS04,2022-2023MS03,2022-2023MS010)the Science and Technology Research Program of Chongqing Education Commission(China)(No.KJQN202302811).
文摘Mantle cell lymphoma(MCL)is recognized as one of the most genetically heterogeneous diseases,with high instability at the genomic level.MCL is common in males with a male-to-female ratio of about 2:1,and its incidence accounts for 3%e10%of adult non-Hodgkin lymphoma cases.The evolutionary dynamics of MCL clones at the single-cell level remain largely unclear.Our research suggests that MCL may arise from multiple cells within the abnormal microenvironment of the entire hematopoietic lineage,particularly from initiating cells.These initiating cells predominantly consist of CD19^(-)/IgM^(-) subclones and exhibit a disrupted malignant clonal differentiation of pre-B cells along the tumor immunity evolution tree.
文摘Multiplexed fluorescence measurement at the singlemolecule level provides unique insight into the molecular composition and interaction within a complex system.1,2 However,fluorescence-based detection is typically restricted to 3-4 colors at a time,due to a low signal-to-noise ratio,high spectral overlap,and the need to maintain chemical compatibility of dyes.Although chemically diverse fluorophores offer a potentially broad spectroscopic palette,further multiplexing is ultimately constrained by the chemical compatibility and varying labeling performance of different fluorophores.
文摘Background For young patients,the surgical method for lumbar disc herniation remains controversial.The aim of this study was to prospectively determine the short-term clinical outcome after surgery for young patients with lumbar disc herniation.Methods In this prospective comparative study between April 2010 and August 2011,a total of 80 patients underwent primary surgery at a single level for lumbar disc herniation.The patients were divided into two groups：decompression alone and decompression with instrumented fusion.An independent examiner clinically evaluated the patients at preoperation and at 1,3,6,and 12 months after surgery.The patients filled out the instruments for back and leg pain using a Visual Analog Scale （VAS）,Oswestry Low Back Pain Disability Questionnaire （ODI）,and Japanese Orthopaedic Association （JOA） scores.The differences between the two groups were analyzed.Results The mean age of all the patients at the time of surgery was 33.7 years.Of the 80 patients,38 patients underwent decompression alone and 42 patients underwent posterior lumbar interbody fusion.Increasing complexity of surgery was associated with a longer surgery time,greater blood loss,and a longer hospital stay after surgery.Both methods of surgery independently improved outcomes compared with baseline status based on VAS,ODI,and JOA scores （P 〈0.05）,and no significant differences were found between the two groups at most of the measuring points in time,although patients with decompression alone had a higher JOA score （P=0.016） and higher JOA recovery rate （P=0.010） at the 3-month follow-up.Conclusions The short-term results of our study showed that both methods of surgery obtained effective clinical outcomes,but decompression alone had some advantages （shorter surgery time,less blood loss,shorter hospital stay,and lower cost） compared with decompression with instrumented fusion.Young patients with decompression alone could achieve great physical function earlier.
基金supported by the National Natural Science Foundation of China (21421004, 21777041, 21327807)the Program of Introducing Talents of Discipline to Universities (B16017)+1 种基金Innovation Program of Shanghai Municipal Education Commission (2017-0107-00-02-E00023)the Fundamental Research Funds for the Central Universities (222201718001, 222201717003)
文摘Monitoring subtle changes in ionic current flow through a nanopore could be applied to observe single molecule reaction. Here,we introduced cysteine to substitute for lysine at position 238 constructing a mutant aerolysin K238 C. It could be regarded as a nanoreactor to efficiently visualize chemical bonds making and breaking. The compound 5,5′-dithiobis-(2-nitrobenzoic acid)(DTNB) was selected as a reactant coming into collisions on the interface of the pore to occur a reversible reaction. Our results showed that the mutant aerolysin could respond to three molecules of DTNB simultaneously and reflect corresponding levels with distinguishable current signals. Therefore, this method constitutes a simple, generic tool for monitoring single molecule reaction, which evokes a guidance for the mutant aerolysin towards the application of tracking other more reactions at single molecule level.
基金support from CNBM (China National Building Materials) Group for its partial financial support of the work
文摘The transition among multiple charging states of a semiconductor＇s localized intrinsic/impurity defects is considered as phase transitions, and the concept of transition Gibbs free energy level （TGFEL） is proposed. Dependence of the cross section of TGFEL on its charge state is discussed. Introduction of TGFEL to replace acti- vation energy has fundamentally important consequences for semiconductor physics and devices. TGFEL involves entropy. What is to be included and not included in the entropy term consistently for all defect levels is an unre- solved open question, related to correct interpretation of various experimental data associated with various defect levels. This work is a first step towards resolving this question.
