4PU—30[N—phenyl—’N—(2—chloro—4—pyridyl) urea] is a new type of plant growth regulator with cytokinin properties. It has been confirmed to delay rice leaf senescence effectively. In order to elucidate the physi...4PU—30[N—phenyl—’N—(2—chloro—4—pyridyl) urea] is a new type of plant growth regulator with cytokinin properties. It has been confirmed to delay rice leaf senescence effectively. In order to elucidate the physiological role of 4PU—30 in delaying senescence, the changes of protein, nucleic acid contents, and the related activities of degradative enzymes were studied. Shanyou 63, an indica hybrid rice was used for this experiment. In the in vitro experiment, two full—developed leaves from the top during heading stage were collected and cut into 5.0cm segments, They were floated on the surface of distilled water containing 0.1mg/14PU—30 and incubated in darkness at 30 C. The leaves floated on distilled water were used as control.It was observed that chlorophyll content in controlled leaves declined rapidly started from the second day and dropped by 93.4% on the 6th day while that in leaves treated with 4PU—30 declined by 41.4% only. During senescence, specific activities of hemoglobin—digesting展开更多
The pot experiment was designed to investigate therelationship between the senescence of hybrid riceand the irrigation mode and conducted during1984-1985.There were 30 pots for each treatmentand each pot contained 17....The pot experiment was designed to investigate therelationship between the senescence of hybrid riceand the irrigation mode and conducted during1984-1985.There were 30 pots for each treatmentand each pot contained 17.5 kg soil.Hybrid riceShanyou 6 was sown on 10 Jun ahd transplanted展开更多
Chronic nephritis management remains challenging due to the compromised therapeutic efficacy and severe systemic complications of conventional glucocorticoid therapy.Here,we developed a bioinspired plateletmediated de...Chronic nephritis management remains challenging due to the compromised therapeutic efficacy and severe systemic complications of conventional glucocorticoid therapy.Here,we developed a bioinspired plateletmediated delivery system(LN-DEX@PLT)that leverages platelet tropism toward injured glomeruli for preci-sion drug delivery.This system integrates lipid nanoemulsion encapsulation with platelet-mediated hitchhiking delivery to achieve three key functionalities:(1)enhanced renal targeting demonstrated by 2.2-fold higher glomerular accumulation compared to free dexamethasone via In vivo imaging,(2)effective mitigation of glucocorticoid-induced metabolic toxicity evidenced by reduced fasting plasma glucose(5.2±0.3 vs 8.3±0.7 mmol/L in free DEX),suppression of hepatic gluconeogenic enzymes(PEPCK expression decreased by 43%,G-6 Pase by 51%,both p<0.001),and suppressed body weight(23.1%versus free DEX group),and(3)dual-pathway therapeutic effects through IL-6/TNF-αsuppression and p53-p21Cip1-mediated senescence delay.In Adriamycin-based chronic nephritis models,LN-DEX@PLT demonstrated superior renal protection with 81%reduction in proteinuria(vs 33%for free DEX).In LPS-induced and Adriamycin-based chronic nephritis models,LN-DEX@PLT demonstrated suppression of renal inflammation markers(IL-6 expression decreased to 68%,TNFαto 51%)and macrophage infiltration(F4/80+cells decreased 5.3-fold).This platelet-biohybrid system pro-vides a clinically translatable paradigm for precision glucocorticoid therapy with reduced dosing frequency.展开更多
文摘4PU—30[N—phenyl—’N—(2—chloro—4—pyridyl) urea] is a new type of plant growth regulator with cytokinin properties. It has been confirmed to delay rice leaf senescence effectively. In order to elucidate the physiological role of 4PU—30 in delaying senescence, the changes of protein, nucleic acid contents, and the related activities of degradative enzymes were studied. Shanyou 63, an indica hybrid rice was used for this experiment. In the in vitro experiment, two full—developed leaves from the top during heading stage were collected and cut into 5.0cm segments, They were floated on the surface of distilled water containing 0.1mg/14PU—30 and incubated in darkness at 30 C. The leaves floated on distilled water were used as control.It was observed that chlorophyll content in controlled leaves declined rapidly started from the second day and dropped by 93.4% on the 6th day while that in leaves treated with 4PU—30 declined by 41.4% only. During senescence, specific activities of hemoglobin—digesting
文摘The pot experiment was designed to investigate therelationship between the senescence of hybrid riceand the irrigation mode and conducted during1984-1985.There were 30 pots for each treatmentand each pot contained 17.5 kg soil.Hybrid riceShanyou 6 was sown on 10 Jun ahd transplanted
基金supported by a research grant from the Natural Sci-ence Foundation of China(82470762 and 82170750)the Foundation of Jiangsu Provincial Research Hospital(YJXYY202204).
文摘Chronic nephritis management remains challenging due to the compromised therapeutic efficacy and severe systemic complications of conventional glucocorticoid therapy.Here,we developed a bioinspired plateletmediated delivery system(LN-DEX@PLT)that leverages platelet tropism toward injured glomeruli for preci-sion drug delivery.This system integrates lipid nanoemulsion encapsulation with platelet-mediated hitchhiking delivery to achieve three key functionalities:(1)enhanced renal targeting demonstrated by 2.2-fold higher glomerular accumulation compared to free dexamethasone via In vivo imaging,(2)effective mitigation of glucocorticoid-induced metabolic toxicity evidenced by reduced fasting plasma glucose(5.2±0.3 vs 8.3±0.7 mmol/L in free DEX),suppression of hepatic gluconeogenic enzymes(PEPCK expression decreased by 43%,G-6 Pase by 51%,both p<0.001),and suppressed body weight(23.1%versus free DEX group),and(3)dual-pathway therapeutic effects through IL-6/TNF-αsuppression and p53-p21Cip1-mediated senescence delay.In Adriamycin-based chronic nephritis models,LN-DEX@PLT demonstrated superior renal protection with 81%reduction in proteinuria(vs 33%for free DEX).In LPS-induced and Adriamycin-based chronic nephritis models,LN-DEX@PLT demonstrated suppression of renal inflammation markers(IL-6 expression decreased to 68%,TNFαto 51%)and macrophage infiltration(F4/80+cells decreased 5.3-fold).This platelet-biohybrid system pro-vides a clinically translatable paradigm for precision glucocorticoid therapy with reduced dosing frequency.
