The realization of protein functional movement is usually accompanied by specific conformational changes,and there exist some key residues that mediate and control the functional motions of proteins in the allosteric ...The realization of protein functional movement is usually accompanied by specific conformational changes,and there exist some key residues that mediate and control the functional motions of proteins in the allosteric process.In the present work,the perturbation-response scanning method developed by our group was combined with the molecular dynamics(MD)simulation to identify the key residues controlling the functional movement of proteins.In our method,a physical quantity that is directly related to protein specific function was introduced,and then based on the MD simulation trajectories,the perturbation-response scanning method was used to identify the key residues for functional motions,in which the residues that highly correlated with the fluctuation of the function-related quantity were identified as the key residues controlling the specific functional motions of the protein.Two protein systems,i.e.,the heat shock protein 70 and glutamine binding protein,were selected as case studies to validate the effectiveness of our method.Our calculated results are in good agreement with the experimental results.The location of the key residues in the two proteins are similar,indicating the similar mechanisms behind the performance of their biological functions.展开更多
The intrusion of obstacles onto railway tracks presents a significant threat to train safety,characterized by sudden and unpredictable occurrences.With China leading the world in high-speed rail mileage,ensuring railw...The intrusion of obstacles onto railway tracks presents a significant threat to train safety,characterized by sudden and unpredictable occurrences.With China leading the world in high-speed rail mileage,ensuring railway security is paramount.The current laser monitoring technologies suffer from high false alarm rates and unreliable intrusion identification.This study addresses these issues by investigating high-resolution laser monitoring technology for railway obstacles,focusing on key parameters such as monitoring range and resolution.We propose an enhanced non-uniform laser scanning method,developing a laser monitoring system that reduces the obstacle false alarm rate to 2.00%,significantly lower than the 20%standard(TJ/GW135-2015).This rate is the best record for laser monitoring systems on China Railway.Our system operates seamlessly in all weather conditions,providing superior accuracy,resolution,and identification efficiency.It is the only 3D LiDAR system certified by the China State Railway Group Co.,Ltd.(Certificate No.[2023]008).Over three years,our system has been deployed at numerous points along various lines managed by the China State Railway Group,accumulating a dataset of 300,000 observations.This extensive deployment has significantly enhanced railway safety.The development and implementation of our railway laser monitoring system represent a substantial advancement in railway safety technology.Its low false alarm rate(2.00%),high accuracy(20 cm×20 cm×20 cm),and robust performance in diverse conditions underscore its potential for widespread adoption,promising to enhance railway safety in China and internationally.展开更多
A mathematical model of deterndulng sound power by using the scanning method is developed. It is assumed that the scanning speed is constant and the noise source is stationary The accuracy of estimating sound power al...A mathematical model of deterndulng sound power by using the scanning method is developed. It is assumed that the scanning speed is constant and the noise source is stationary The accuracy of estimating sound power along some simple paths on the surfaces such as rectangle, disc and hemisphere is analyzed. It is argued that the accuracy of estimating sound power is strongly depended on a suitable selection of scan path. The accurate estdriation of sound power can be made by scanning along some simple paths.展开更多
Plasma spraying-physical vapor deposition(PS-PVD)and atmospheric plasma spraying(APS),as new thermal spraying process,could prepare advanced thermal barrier coatings by successive scanning deposition layer by layer.To...Plasma spraying-physical vapor deposition(PS-PVD)and atmospheric plasma spraying(APS),as new thermal spraying process,could prepare advanced thermal barrier coatings by successive scanning deposition layer by layer.To investigate the layer structure of the coating,the transient in-situ deposit surface temperature was investigated by varying the scanning methods.Results show that,compared to unidirectional scanning method,alternating scanning method results in doubling heating duration,higher in-situ deposit temperature rising and thereby thicker well-bonded layer structure during each spray cycle for the coating deposited at the edge position of the scanning path.The erosion resistance of the coatings deposited by unidirectional scanning method is independent on deposition positions including edge position and center position.However,the erosion resistance of the coatings deposited at edge position by alternating scanning method is one time higher than those deposited by unidirectional scanning method.Finally,the erosion mechanism of layer spallation is revealed to explain the difference between different scanning methods.展开更多
Five phospholipids in human placenta were determined by phosphorus 31 nuclear magnetic resonance(^(31)P NMR)spectroscopy and thin-layer chromatography(TLC) scanning combined with the corrective method of absorbance pr...Five phospholipids in human placenta were determined by phosphorus 31 nuclear magnetic resonance(^(31)P NMR)spectroscopy and thin-layer chromatography(TLC) scanning combined with the corrective method of absorbance proportional coefficient. The NMR spectrometer used this investigation was a Bruker AM-500 spectrometer operating at 202.4 MHz for ^(31)P chemical shifts are relative to 85% phosphoric acid. TIC was carried out by silica gel H plate developed in chloroform-methanol-glacial acetic acid-ethanol-water(25:4:6:2:0.5),with Vaskovsky reagent as colour -developing agent of phospholipids.展开更多
We use the couple dipole method to investigate the scanning near-field optical microscopy metallic tip-nanoparticle near-field interaction. Dependences of the local field intensity inside the nanoparticle on the nanos...We use the couple dipole method to investigate the scanning near-field optical microscopy metallic tip-nanoparticle near-field interaction. Dependences of the local field intensity inside the nanoparticle on the nanosized tip shape,the tip open angle and the illumination angle are revealed. In combination with the previous results, we establish a complete model to understand the tip-nanoparticle near-field coupling mechanism.展开更多
A space laser communication acquisition,pointing and tracking(APT)system based on the beacon laser is designed without prior information.And then,a new target scanning method and a pointing and tracking algorithm are ...A space laser communication acquisition,pointing and tracking(APT)system based on the beacon laser is designed without prior information.And then,a new target scanning method and a pointing and tracking algorithm are proposed.The target scanning mode is the round-trip triangular wave scanning,and it means that scanning track of the PAN-TILT platform follows the triangular wave repeatedly.For the pointing and tracking algorithm,the beacon laser is used as the auxiliary aiming light source.The position of the beacon laser in the viewfield of the complementary metal oxide semiconductor(CMOS)camera is calculated by the centroid algorithm.In order to realize the target tracking,the joint control method of the angle control and the angular velocity control is used.The simulation and experimental results show that the APT system can achieve full coverage scanning in the scanning area and capture the target in one scanning cycle successfully.After capturing the PAN-TILT platform,the pointing and tracking algorithm can track the PAN-TILT platform quickly and accurately,and the tracking accuracy is up to 0.22 mrad.展开更多
A scanning Hartmann test method is proposed and its measurement principle is described. The scanning Hartmann test setup is formed by modifying the Hartmann screen of the conventional Hartmann test setup. With the rot...A scanning Hartmann test method is proposed and its measurement principle is described. The scanning Hartmann test setup is formed by modifying the Hartmann screen of the conventional Hartmann test setup. With the rotation of the scanning Hartmann screen and the improved hole arrangement, the whole information of the lens to be tested in the full aperture can be obtained. The measurement accuracy of the aberration is improved and the local error of the lens can be got. In the method, no change of the Hartmann screen is needed for measuring the lenses of different aperture sizes. Experimental results of aberration measurements of two lenses are given to verify the usefulness of the setup.展开更多
Oil saturation is a critical parameter when designing oil field development plans.This study focuses on the change of oil saturation during water flooding.Particularly,a meter-level artificial model is used to conduct...Oil saturation is a critical parameter when designing oil field development plans.This study focuses on the change of oil saturation during water flooding.Particularly,a meter-level artificial model is used to conduct relevant experiments on the basis of similarity principles and taking into account the layer geological characteristics of the reservoir.The displacement experiment’s total recovery rate is 41.35%.The changes in the remaining oil saturation at a millimeter-scale are examined using medical spiral computer tomography principles.In all experimental stages,regions exists where the oil saturation decline is more than 10.0%.The shrinkage percentage is 20.70%in the horizontal well production stage.The oil saturation reduction in other parts is less than 10.0%,and there are regions where the oil saturation increases in the conventional water flooding stage.展开更多
文摘The realization of protein functional movement is usually accompanied by specific conformational changes,and there exist some key residues that mediate and control the functional motions of proteins in the allosteric process.In the present work,the perturbation-response scanning method developed by our group was combined with the molecular dynamics(MD)simulation to identify the key residues controlling the functional movement of proteins.In our method,a physical quantity that is directly related to protein specific function was introduced,and then based on the MD simulation trajectories,the perturbation-response scanning method was used to identify the key residues for functional motions,in which the residues that highly correlated with the fluctuation of the function-related quantity were identified as the key residues controlling the specific functional motions of the protein.Two protein systems,i.e.,the heat shock protein 70 and glutamine binding protein,were selected as case studies to validate the effectiveness of our method.Our calculated results are in good agreement with the experimental results.The location of the key residues in the two proteins are similar,indicating the similar mechanisms behind the performance of their biological functions.
基金financially supported by the National Natural Science Foundation of China(Nos.62275244,62375258,62225507,U2033211,62175230,and 62175232)the CAS Project for Young Scientists in Basic Research(No.YSBR-065)+2 种基金Scientific Instrument Developing Project of the Chinese Academy of Sciences(No.YJKYYQ20200001)National Key R&D Program of China(No.2022YFB3607800,No.2022YFB3605800,and No.2022YFB4601501)Key Program of the Chinese Academy of Sciences(ZDBS-ZRKJZ-TLC018)。
文摘The intrusion of obstacles onto railway tracks presents a significant threat to train safety,characterized by sudden and unpredictable occurrences.With China leading the world in high-speed rail mileage,ensuring railway security is paramount.The current laser monitoring technologies suffer from high false alarm rates and unreliable intrusion identification.This study addresses these issues by investigating high-resolution laser monitoring technology for railway obstacles,focusing on key parameters such as monitoring range and resolution.We propose an enhanced non-uniform laser scanning method,developing a laser monitoring system that reduces the obstacle false alarm rate to 2.00%,significantly lower than the 20%standard(TJ/GW135-2015).This rate is the best record for laser monitoring systems on China Railway.Our system operates seamlessly in all weather conditions,providing superior accuracy,resolution,and identification efficiency.It is the only 3D LiDAR system certified by the China State Railway Group Co.,Ltd.(Certificate No.[2023]008).Over three years,our system has been deployed at numerous points along various lines managed by the China State Railway Group,accumulating a dataset of 300,000 observations.This extensive deployment has significantly enhanced railway safety.The development and implementation of our railway laser monitoring system represent a substantial advancement in railway safety technology.Its low false alarm rate(2.00%),high accuracy(20 cm×20 cm×20 cm),and robust performance in diverse conditions underscore its potential for widespread adoption,promising to enhance railway safety in China and internationally.
文摘A mathematical model of deterndulng sound power by using the scanning method is developed. It is assumed that the scanning speed is constant and the noise source is stationary The accuracy of estimating sound power along some simple paths on the surfaces such as rectangle, disc and hemisphere is analyzed. It is argued that the accuracy of estimating sound power is strongly depended on a suitable selection of scan path. The accurate estdriation of sound power can be made by scanning along some simple paths.
基金supported by the National Natural Science Foundation of China(Grant No.51901175)the China Postdoctoral Science Foundation funded project(No.2020T130499)the National Program for Support of Top-notch Young Professionals.
文摘Plasma spraying-physical vapor deposition(PS-PVD)and atmospheric plasma spraying(APS),as new thermal spraying process,could prepare advanced thermal barrier coatings by successive scanning deposition layer by layer.To investigate the layer structure of the coating,the transient in-situ deposit surface temperature was investigated by varying the scanning methods.Results show that,compared to unidirectional scanning method,alternating scanning method results in doubling heating duration,higher in-situ deposit temperature rising and thereby thicker well-bonded layer structure during each spray cycle for the coating deposited at the edge position of the scanning path.The erosion resistance of the coatings deposited by unidirectional scanning method is independent on deposition positions including edge position and center position.However,the erosion resistance of the coatings deposited at edge position by alternating scanning method is one time higher than those deposited by unidirectional scanning method.Finally,the erosion mechanism of layer spallation is revealed to explain the difference between different scanning methods.
文摘Five phospholipids in human placenta were determined by phosphorus 31 nuclear magnetic resonance(^(31)P NMR)spectroscopy and thin-layer chromatography(TLC) scanning combined with the corrective method of absorbance proportional coefficient. The NMR spectrometer used this investigation was a Bruker AM-500 spectrometer operating at 202.4 MHz for ^(31)P chemical shifts are relative to 85% phosphoric acid. TIC was carried out by silica gel H plate developed in chloroform-methanol-glacial acetic acid-ethanol-water(25:4:6:2:0.5),with Vaskovsky reagent as colour -developing agent of phospholipids.
基金Supported by the Start-Up Grant of Zhejiang University of Technology,the Zhejiang Provincial Key Laboratory of Information Processing,Communication and Networking,the Zhejiang University,and the National Natural Science Foundation of China under Grant No 61605171
文摘We use the couple dipole method to investigate the scanning near-field optical microscopy metallic tip-nanoparticle near-field interaction. Dependences of the local field intensity inside the nanoparticle on the nanosized tip shape,the tip open angle and the illumination angle are revealed. In combination with the previous results, we establish a complete model to understand the tip-nanoparticle near-field coupling mechanism.
基金National Natural Science Foundation of China(No.52173219)。
文摘A space laser communication acquisition,pointing and tracking(APT)system based on the beacon laser is designed without prior information.And then,a new target scanning method and a pointing and tracking algorithm are proposed.The target scanning mode is the round-trip triangular wave scanning,and it means that scanning track of the PAN-TILT platform follows the triangular wave repeatedly.For the pointing and tracking algorithm,the beacon laser is used as the auxiliary aiming light source.The position of the beacon laser in the viewfield of the complementary metal oxide semiconductor(CMOS)camera is calculated by the centroid algorithm.In order to realize the target tracking,the joint control method of the angle control and the angular velocity control is used.The simulation and experimental results show that the APT system can achieve full coverage scanning in the scanning area and capture the target in one scanning cycle successfully.After capturing the PAN-TILT platform,the pointing and tracking algorithm can track the PAN-TILT platform quickly and accurately,and the tracking accuracy is up to 0.22 mrad.
文摘A scanning Hartmann test method is proposed and its measurement principle is described. The scanning Hartmann test setup is formed by modifying the Hartmann screen of the conventional Hartmann test setup. With the rotation of the scanning Hartmann screen and the improved hole arrangement, the whole information of the lens to be tested in the full aperture can be obtained. The measurement accuracy of the aberration is improved and the local error of the lens can be got. In the method, no change of the Hartmann screen is needed for measuring the lenses of different aperture sizes. Experimental results of aberration measurements of two lenses are given to verify the usefulness of the setup.
基金supported by the National Science and Technology Major Projects of China for Oil and Gas(Projects Nos.2016ZX05010 and 2016ZX05058).
文摘Oil saturation is a critical parameter when designing oil field development plans.This study focuses on the change of oil saturation during water flooding.Particularly,a meter-level artificial model is used to conduct relevant experiments on the basis of similarity principles and taking into account the layer geological characteristics of the reservoir.The displacement experiment’s total recovery rate is 41.35%.The changes in the remaining oil saturation at a millimeter-scale are examined using medical spiral computer tomography principles.In all experimental stages,regions exists where the oil saturation decline is more than 10.0%.The shrinkage percentage is 20.70%in the horizontal well production stage.The oil saturation reduction in other parts is less than 10.0%,and there are regions where the oil saturation increases in the conventional water flooding stage.
