microRNAs （miRNAs） play an important regulatory role in the self-renewal and differentiation of stem cells. In this study, we examined the effects of miRNA-124 （miR-124） overexpression in bone marrow-derived mesen...microRNAs （miRNAs） play an important regulatory role in the self-renewal and differentiation of stem cells. In this study, we examined the effects of miRNA-124 （miR-124） overexpression in bone marrow-derived mesenchymal stem cells. In particular, we focused on the effect of overexpression on the differentiation of bone marrow-derived mesenchymal stem cells into neurons. First, we used GeneChip technology to analyze the expression of miRNAs in bone marrow-derived mesen- chymal stem cells, neural stem cells and neurons, miR-124 expression was substantially reduced in bone marrow-derived mesenchymal stem cells compared with the other cell types. We con- structed a lentiviral vector overexpressing miR-124 and transfected it into bone marrow-derived mesenchymal stem cells. Intracellular expression levels of the neuronal early markers [3-III tu- bulin and microtubule-associated protein-2 were significantly increased, and apoptosis induced by oxygen and glucose deprivation was reduced in transfected cells. After miR-124-transfected bone marrow-derived mesenchymal stem cells were transplanted into the injured rat spinal cord, a large number of cells positive for the neuronal marker neurofilament-200 were observed in the transplanted region. The Basso-Beattie-Bresnahan locomotion scores showed that the motor function of the hind limb of rats with spinal cord injury was substantially improved. These re- sults suggest that miR-124 plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells into neurons. Our findings should facilitate the development of novel strategies for enhancing the therapeutic efficacy of bone marrow-derived mesenchymal stem cell transplantation for spinal cord injury.展开更多
Incisional hernias are a prevalent problem in abdominal surgery and occur in 11% of patients who undergo laparotomy. Primary suture clo-sure of incisional hernias results in a 31%-58% chance of recurrence. The additio...Incisional hernias are a prevalent problem in abdominal surgery and occur in 11% of patients who undergo laparotomy. Primary suture clo-sure of incisional hernias results in a 31%-58% chance of recurrence. The addition of a pros-thetic mesh implant decreases recurrence rates to 8%-10%. Popularized in Europe by Rives and Stoppa, the sublay technique has proven to be very effective, with low recurrence rates (0%-23%) and minimal complications. The pur-pose of the study was to evaluate the experi-ence of a single surgeon at a large tertiary care center performing a modified Rives-Stoppa re-pair for abdominal incisional hernias. To do this, the records of all patients undergoing a modi-fied Rives-Stoppa incisional hernia repair be-tween January 2000 and August 2003 were ret-rospectively reviewed. Outpatient clinic notes, discharge summaries, operative reports, and laboratory data were reviewed for patient demographics, surgical data and postoperative complications. Univariate analysis was per-formed in order to identify predictors for recur-rence. During the study period, 83 patients un-derwent a modified Rives-Stoppa incisional hernia repair. Nineteen patients were excluded due to incomplete medical records. No patients required postoperative exploration for an in-tra-abdominal catastrophe. Twenty-five percent (n=16) of patients had a complication as a result of the hernia repair. Only two patients (3.1%) developed recurrent incisional hernias. History of diabetes (p=0.007) and benign prostatic hy-perplasia (p=0.000) were the only significant predictors for recurrence. The results presented here confirm that the modified Rives-Stoppa retromuscular repair is an effective method for the repair of incisional hernias. The complica-tion and recurrence rates compare favorably to results for currently popular alternative tech-niques.展开更多
A model combining basin management and administrative management is currently adopted for the lake and river management in China. In applications,the model suffers many problems,including the confusion of rights and r...A model combining basin management and administrative management is currently adopted for the lake and river management in China. In applications,the model suffers many problems,including the confusion of rights and responsibilities of river basin authorities and the dominant governmental agencies,and the lack of effective public participation. In this paper,we employ the Great Lakes Basin in the United States,the Loire-Brittany River Basin in France,the Southeast Basin in the United Kingdom,the Murray-Darling Basin in Australia,and general German model as examples to analyze and summarize the characteristics of their river and lake management modes,regarding river basin authorities,dominant governmental agencies,public participation,and basin management. This study may provide some referable experience for the integrated river basin management in China.展开更多
Objectives To investigate the effects of testosterone enanthate (TE) on serum lip- ids and lipoproteins metabolism and the expression of androgen receptor (AR) , estrogen receptor beta ( ER - β) and platelet derived ...Objectives To investigate the effects of testosterone enanthate (TE) on serum lip- ids and lipoproteins metabolism and the expression of androgen receptor (AR) , estrogen receptor beta ( ER - β) and platelet derived growth factor beta ( PDGFR - β) in aortic vascular smooth muscle tissues (VSMTs). Methods Forty aged male rats were ran- domly divided into 4 groups, group A (placebo group) , group B (2. 5 mg/kg intramuscular injection of TE once a week ) , group C (5.0 mg/kg intramuscular injection of TE once a week ) , group D ( 10. 0 mg/kg intramus- cular injection of TE once a week). All animals were fed freely during 16 - week treatment periods. The ex- pression of AR ,ER - βand PDGFR - β were studied by Western bolt. Results Average serum LDL - C was lower in group D than that in group A ( p < 0. 01 ). Compared with the other groups, average serum TC was also lower in group D (p <0. 05). AR expression in aortic vascular smooth muscle tissues could be regulated by TE: 99.50 ±21.74, 125.38 ±28.68 and 101.98 ± 15.42 for TE concentrations at 2.5 mg/kg, 5.0 mg/kg and 10.0 mg/kg, respectively , the expression of ER - β could be regulated by TE: 92. 34 ± 18. 68, 47. 72 ± 18.12, 82.13 ±23.50, and the expression of PDGFR - β could be regulated as well by TE: 219.70 ±45. 59, 50.16 ±9. 72, 125.36 ±15. 74(Data for AR ,ER-β and PDGFR - β protein band intensity were expressed with x ± s, with control group taken as 100 ).Conclusions This study indicates that androgens have significant effects on serum lipids and lipoprotein metabolism. Testosterone enanthate at 5. 0 mg/kg can stimulate the expression of AR, but inhibite the expres- sion of PDGFR. Testosterone enanthate at the concen- trations of 5. 0 mg/kg and 10. 0 mg/kg can inhibite the expression of ER - β.展开更多
A mathematical model of river be d change downstream of the Xiaolangdi Reservoir was developed based on the most recent achievement of sediment theory in the Yellow River. The model was verified by the comparison of...A mathematical model of river be d change downstream of the Xiaolangdi Reservoir was developed based on the most recent achievement of sediment theory in the Yellow River. The model was verified by the comparison of computed results and measured data from 1986 to 1996. Num erical prediction of the erosion and deposition downstream of the Xiaolangdi Res ervoir in its first operation year was carried out, and a series of suggestions were given for reservoir operation mode in its early operation period.展开更多
目的探讨重复经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)联合舍曲林治疗抑郁症伴自杀意念的临床疗效。方法选择2022年2月~2024年1月98例抑郁症伴自杀意念患者,采用数字表法随机分为两组,对照组48例,观察组50例。...目的探讨重复经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)联合舍曲林治疗抑郁症伴自杀意念的临床疗效。方法选择2022年2月~2024年1月98例抑郁症伴自杀意念患者,采用数字表法随机分为两组,对照组48例,观察组50例。对照组采用舍曲林治疗,观察组采用rTMS联合舍曲林治疗。评估自杀意念量表(scale for suicidal ideation,SSI)、汉密尔顿抑郁量表(hamilton depression scale,HAMD)、汉密尔顿焦虑量表(hamilton anxiety scale,HAMA),检测血清神经营养因子、神经递质水平。结果两组治疗后SSI评分、HAMD评分、HAMA评分均下降,且治疗后观察组较对照组显著降低(P<0.05);治疗后两组血清脑源性神经营养因子、5-羟色胺(5-hydroxytryptamine,5-HT)、去甲肾上腺素(norepinephrine,NE)水平均显著升高,且治疗后观察组更高(P<0.05)。结论rTMS联合舍曲林治疗抑郁症伴自杀意念能够有效减轻自杀意念以及负性情绪,并改善脑源性神经营养因子以及相关神经递质水平。展开更多
选育高产氮高效水稻品种是解决资源短缺和环境污染问题的有效策略。在宁夏引黄灌区通过大田试验结合主成分分析法,对39个品种/品系在3个氮水平(0、180和240 kg N/hm^(2))下进行研究筛选,成熟期测定各品种(系)的产量、氮素利用效率等指标...选育高产氮高效水稻品种是解决资源短缺和环境污染问题的有效策略。在宁夏引黄灌区通过大田试验结合主成分分析法,对39个品种/品系在3个氮水平(0、180和240 kg N/hm^(2))下进行研究筛选,成熟期测定各品种(系)的产量、氮素利用效率等指标,通过主成分分析法得到各品种(系)的氮效率综合值,并采用产量-氮效率综合指数对供试品种(系)进行产量及氮效率类型划分。结果表明,根据水稻氮效率综合评分与产量关系,将供试品种(系)划分为4个类型,即双高效型、高氮低效型、双低效型和低氮高效型。筛选出宁粳41号、吉粳105、宁粳45号、宁粳47号、宁粳48号、宁粳52号、宁粳55号、宁粳56号、宁粳57号、中科804和花147共11个品种为氮高效品种(系)。与氮低效类型相比,氮高效品种(系)产量增加主要因为穗长、有效穗数及每穗实粒数增加。展开更多
基金supported by the National Natural Science Foundation of China,No.81070971
文摘microRNAs （miRNAs） play an important regulatory role in the self-renewal and differentiation of stem cells. In this study, we examined the effects of miRNA-124 （miR-124） overexpression in bone marrow-derived mesenchymal stem cells. In particular, we focused on the effect of overexpression on the differentiation of bone marrow-derived mesenchymal stem cells into neurons. First, we used GeneChip technology to analyze the expression of miRNAs in bone marrow-derived mesen- chymal stem cells, neural stem cells and neurons, miR-124 expression was substantially reduced in bone marrow-derived mesenchymal stem cells compared with the other cell types. We con- structed a lentiviral vector overexpressing miR-124 and transfected it into bone marrow-derived mesenchymal stem cells. Intracellular expression levels of the neuronal early markers [3-III tu- bulin and microtubule-associated protein-2 were significantly increased, and apoptosis induced by oxygen and glucose deprivation was reduced in transfected cells. After miR-124-transfected bone marrow-derived mesenchymal stem cells were transplanted into the injured rat spinal cord, a large number of cells positive for the neuronal marker neurofilament-200 were observed in the transplanted region. The Basso-Beattie-Bresnahan locomotion scores showed that the motor function of the hind limb of rats with spinal cord injury was substantially improved. These re- sults suggest that miR-124 plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells into neurons. Our findings should facilitate the development of novel strategies for enhancing the therapeutic efficacy of bone marrow-derived mesenchymal stem cell transplantation for spinal cord injury.
文摘Incisional hernias are a prevalent problem in abdominal surgery and occur in 11% of patients who undergo laparotomy. Primary suture clo-sure of incisional hernias results in a 31%-58% chance of recurrence. The addition of a pros-thetic mesh implant decreases recurrence rates to 8%-10%. Popularized in Europe by Rives and Stoppa, the sublay technique has proven to be very effective, with low recurrence rates (0%-23%) and minimal complications. The pur-pose of the study was to evaluate the experi-ence of a single surgeon at a large tertiary care center performing a modified Rives-Stoppa re-pair for abdominal incisional hernias. To do this, the records of all patients undergoing a modi-fied Rives-Stoppa incisional hernia repair be-tween January 2000 and August 2003 were ret-rospectively reviewed. Outpatient clinic notes, discharge summaries, operative reports, and laboratory data were reviewed for patient demographics, surgical data and postoperative complications. Univariate analysis was per-formed in order to identify predictors for recur-rence. During the study period, 83 patients un-derwent a modified Rives-Stoppa incisional hernia repair. Nineteen patients were excluded due to incomplete medical records. No patients required postoperative exploration for an in-tra-abdominal catastrophe. Twenty-five percent (n=16) of patients had a complication as a result of the hernia repair. Only two patients (3.1%) developed recurrent incisional hernias. History of diabetes (p=0.007) and benign prostatic hy-perplasia (p=0.000) were the only significant predictors for recurrence. The results presented here confirm that the modified Rives-Stoppa retromuscular repair is an effective method for the repair of incisional hernias. The complica-tion and recurrence rates compare favorably to results for currently popular alternative tech-niques.
基金Supported by Jiangxi Soft Science Plan Research Item(20151BBA10066)
文摘A model combining basin management and administrative management is currently adopted for the lake and river management in China. In applications,the model suffers many problems,including the confusion of rights and responsibilities of river basin authorities and the dominant governmental agencies,and the lack of effective public participation. In this paper,we employ the Great Lakes Basin in the United States,the Loire-Brittany River Basin in France,the Southeast Basin in the United Kingdom,the Murray-Darling Basin in Australia,and general German model as examples to analyze and summarize the characteristics of their river and lake management modes,regarding river basin authorities,dominant governmental agencies,public participation,and basin management. This study may provide some referable experience for the integrated river basin management in China.
文摘Objectives To investigate the effects of testosterone enanthate (TE) on serum lip- ids and lipoproteins metabolism and the expression of androgen receptor (AR) , estrogen receptor beta ( ER - β) and platelet derived growth factor beta ( PDGFR - β) in aortic vascular smooth muscle tissues (VSMTs). Methods Forty aged male rats were ran- domly divided into 4 groups, group A (placebo group) , group B (2. 5 mg/kg intramuscular injection of TE once a week ) , group C (5.0 mg/kg intramuscular injection of TE once a week ) , group D ( 10. 0 mg/kg intramus- cular injection of TE once a week). All animals were fed freely during 16 - week treatment periods. The ex- pression of AR ,ER - βand PDGFR - β were studied by Western bolt. Results Average serum LDL - C was lower in group D than that in group A ( p < 0. 01 ). Compared with the other groups, average serum TC was also lower in group D (p <0. 05). AR expression in aortic vascular smooth muscle tissues could be regulated by TE: 99.50 ±21.74, 125.38 ±28.68 and 101.98 ± 15.42 for TE concentrations at 2.5 mg/kg, 5.0 mg/kg and 10.0 mg/kg, respectively , the expression of ER - β could be regulated by TE: 92. 34 ± 18. 68, 47. 72 ± 18.12, 82.13 ±23.50, and the expression of PDGFR - β could be regulated as well by TE: 219.70 ±45. 59, 50.16 ±9. 72, 125.36 ±15. 74(Data for AR ,ER-β and PDGFR - β protein band intensity were expressed with x ± s, with control group taken as 100 ).Conclusions This study indicates that androgens have significant effects on serum lipids and lipoprotein metabolism. Testosterone enanthate at 5. 0 mg/kg can stimulate the expression of AR, but inhibite the expres- sion of PDGFR. Testosterone enanthate at the concen- trations of 5. 0 mg/kg and 10. 0 mg/kg can inhibite the expression of ER - β.
基金Project supported by the National Natural Science Foundation of China. (No:59890200)
文摘A mathematical model of river be d change downstream of the Xiaolangdi Reservoir was developed based on the most recent achievement of sediment theory in the Yellow River. The model was verified by the comparison of computed results and measured data from 1986 to 1996. Num erical prediction of the erosion and deposition downstream of the Xiaolangdi Res ervoir in its first operation year was carried out, and a series of suggestions were given for reservoir operation mode in its early operation period.
文摘选育高产氮高效水稻品种是解决资源短缺和环境污染问题的有效策略。在宁夏引黄灌区通过大田试验结合主成分分析法,对39个品种/品系在3个氮水平(0、180和240 kg N/hm^(2))下进行研究筛选,成熟期测定各品种(系)的产量、氮素利用效率等指标,通过主成分分析法得到各品种(系)的氮效率综合值,并采用产量-氮效率综合指数对供试品种(系)进行产量及氮效率类型划分。结果表明,根据水稻氮效率综合评分与产量关系,将供试品种(系)划分为4个类型,即双高效型、高氮低效型、双低效型和低氮高效型。筛选出宁粳41号、吉粳105、宁粳45号、宁粳47号、宁粳48号、宁粳52号、宁粳55号、宁粳56号、宁粳57号、中科804和花147共11个品种为氮高效品种(系)。与氮低效类型相比,氮高效品种(系)产量增加主要因为穗长、有效穗数及每穗实粒数增加。
