Two novel diketopiperazines(1 and 5),along with ten known compounds(2−4,6−12)demonstrating significant skin inflammation inhibition,were isolated from a marine-derived fungus identified as Aspergillus sp.FAZW0001.The ...Two novel diketopiperazines(1 and 5),along with ten known compounds(2−4,6−12)demonstrating significant skin inflammation inhibition,were isolated from a marine-derived fungus identified as Aspergillus sp.FAZW0001.The structural elucidation and configurational reassessments of compounds 1−5 were established through comprehensive spectral analyses,with their absolute configurations determined via single crystal X-ray diffraction using Cu Kαradiation,Marfey’s method,and comparison between experimental and calculated electronic circular dichroism(ECD)spectra.Compounds 1,2,and 8 exhibited significant anti-inflammatory activities in Propionibacterium acnes(P.acnes)-induced human monocyte cell lines.Compound 8 demonstrated the ability to down-regulate interleukin-1β(IL-1β)expression by inhibiting Toll-like receptor 2(TLR2)expression and modulating the activation of myeloid differentiation factor 88(MyD88),mitogen-activated protein kinase(MAPK),and nuclear factorκB(NF-κB)signaling pathways,thus reducing the cellular inflammatory response induced by P.acnes.Additionally,compound 8 showed the capacity to suppress mitochondrial reactive oxygen species(ROS)production and nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)inflammasome activation,thereby reducing IL-1βmaturation and secretion.A three-dimensional quantitative structure-activity relationships(3D-QSAR)model was applied to compounds 5−12 to analyze their anti-inflammatory structure-activity relationships.展开更多
Airport disruptions often pose challenges in assigning aircraft to gates,resulting in infeasible planned schedules.In particular,a large number of transfer passengers miss their connections in the context of disruptio...Airport disruptions often pose challenges in assigning aircraft to gates,resulting in infeasible planned schedules.In particular,a large number of transfer passengers miss their connections in the context of disruptions,which cause huge economic losses to airlines and serious passengers’dissatisfaction.This paper proposes a set-partitioning-based model to optimize Aircraft-Gate Reassignment with Transfer Passenger Connections(AGRP-TPC),which incorporates flexible gate-swap and aircraft-delay operations to mitigate the overall impact of disruptions.To efficiently solve the model,we introduce the concepts of additive-transfer and nonstop-transfer to handle passenger connections,and develop a Hierarchical Column-and-Row Generation(HCRG)approach guided by airport terminal space attribute.The column generation and row generation procedures solve iteratively until no new variables and constraints are generated.In addition,a follow-on strategy and a diving heuristic are designed to efficiently obtain high-quality solutions.We evaluate the proposed approach using various instances from a major Chinese international airport.Computational results demonstrate that our approach outperforms the comparison algorithms and produces good solutions within the time limit.Detailed results indicate that our approach effectively reduces overall losses in aircraft-gate reassignment following disruptions,and it can serve as an auxiliary decision-making tool for airport operators and airlines.展开更多
In this paper, we first analyzed cloud drift wind(CDW) data distribution in the vertical direction, and then reassigned the height of every CDW in the research domain in terms of background information, and finally, c...In this paper, we first analyzed cloud drift wind(CDW) data distribution in the vertical direction, and then reassigned the height of every CDW in the research domain in terms of background information, and finally, conducted contrast numerical experiments of assimilating the CDW data before and after reassignment to examine the impacts on the forecast of the track of Typhoon Chanthu(1003) from 00:00(Coordinated Universal Time) 21 July to 00:00 UTC23 July, 2010. The analysis results of the CDW data indicate that the number of CDWs is mainly distributed in the midand upper-troposphere above 500 h Pa, with the maximum number at about 300 h Pa. The height reassigning method mentioned in this work may update the height effectively, and the CDW data are distributed reasonably and no obvious contradiction occurs in the horizontal direction after height reassignment. After assimilating the height-reassigned CDW data, especially the water vapor CDW data, the initial wind field around Typhoon Chanthu(1003) became more reasonable, and then the steering current leading the typhoon to move to the correct location became stronger. As a result, the numerical track predictions are improved.展开更多
A group of 39 Transgender subjects, 17 Female to Male (FtM) and 22 Male to Female (MtF), have been evaluated about their sexual desire, activity, and satisfaction during the period before and after Sex Reassignment by...A group of 39 Transgender subjects, 17 Female to Male (FtM) and 22 Male to Female (MtF), have been evaluated about their sexual desire, activity, and satisfaction during the period before and after Sex Reassignment by Surgery (SRS). Visual Analogue Scale from 0 to 10 has been used to assess sexual desire and satisfaction, number of sexual partners and number of sexual intercourses/ month have been evaluated to assess sexual activity. Moreover, after the surgery, the Sexual Desire Inventory-2 (SDI-2) was administered to patients and related to testosterone (total or free) levels. In FtM, after hysterectomy and bilateral ovariectomy, mean value of sexual desire (VAS 0 - 10) was 7.5 before beginning testosterone therapy, this value increased after six months of therapy and after one year. Just before SRS the value decreased, but increased to 8.42 after SRS. In the same patients the values (VAS 0 - 10) on sexual satisfaction were respectively 6.5, 6.9, 7.1, 6.5 and 7. Mean number of partners was 4, with 7.07 mean frequency of sexual intercourse/month, before starting therapy (after hysterectomy). During hormonal therapy (testosterone), No. of partners was 2.46 and 8.96 intercourses/month, while after SRS the partners were 1.25 and intercouses/month 7.91. MtF patients reported a sexual desire (VAS 0 - 10) before therapy of 7, this remained almost constant after 6 months, decreases after 12 months and just before surgery, and finally increased to 7.76 after SRS. For sexual satisfaction mean values of VAS were respectively: 6.1, 6.2, 6.3, 6, and 6.8. Regarding sexual activity, MtF report 3.81 mean No. of partners and 9.27 intercouses/month before the beginning of hormonal therapy, 1.36 partners and 4.36 intercourses after therapy, and 1.3 partners and 4.54 intercourses after SRS.展开更多
We present a case of a transsexual patient who underwent a partial pelvectomy and genital reconstruction for anal cancer after chemoradiation. This is the first case in literature reporting on the occurrence of anal c...We present a case of a transsexual patient who underwent a partial pelvectomy and genital reconstruction for anal cancer after chemoradiation. This is the first case in literature reporting on the occurrence of anal cancer after male-to-female sex reassignment surgery. We describe the surgical approach presenting our technique to avoid postoperative complications and preserve the sexual reassignment.展开更多
In light-sheet fluorescence microscopy,the axial resolution and field of view are mutually constrained.Axially swept light-sheet microscopy(ASLM)can decouple the trade-off,but the confocal detection scheme using a rol...In light-sheet fluorescence microscopy,the axial resolution and field of view are mutually constrained.Axially swept light-sheet microscopy(ASLM)can decouple the trade-off,but the confocal detection scheme using a rolling shutter also rejects fluorescence signals from the specimen in the field of interest,which sacrifices the photon efficiency.Here,we report a laterally swept light-sheet microscopy(LSLM)scheme in which the focused beam is first scanned along the axial direction and subsequently laterally swept with the rolling shutter.We show that LSLM can obtain a higher photon efficiency when similar axial resolution and field of view can be achieved.Moreover,based on the principle of image scanning microscopy,applying the pixel reassignment to the LSLM images,hereby named iLSLM,improves the optical sectioning.Both simulation and experimental results demonstrate the higher photon efficiency with similar axial resolution and optical sectioning.Our proposed scheme is suitable for volumetric imaging of specimens that are susceptible to photobleaching or phototoxicity.展开更多
基金supported by the National Natural Science Foundation of China(Nos.41876189 and 81703388)the State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology.
文摘Two novel diketopiperazines(1 and 5),along with ten known compounds(2−4,6−12)demonstrating significant skin inflammation inhibition,were isolated from a marine-derived fungus identified as Aspergillus sp.FAZW0001.The structural elucidation and configurational reassessments of compounds 1−5 were established through comprehensive spectral analyses,with their absolute configurations determined via single crystal X-ray diffraction using Cu Kαradiation,Marfey’s method,and comparison between experimental and calculated electronic circular dichroism(ECD)spectra.Compounds 1,2,and 8 exhibited significant anti-inflammatory activities in Propionibacterium acnes(P.acnes)-induced human monocyte cell lines.Compound 8 demonstrated the ability to down-regulate interleukin-1β(IL-1β)expression by inhibiting Toll-like receptor 2(TLR2)expression and modulating the activation of myeloid differentiation factor 88(MyD88),mitogen-activated protein kinase(MAPK),and nuclear factorκB(NF-κB)signaling pathways,thus reducing the cellular inflammatory response induced by P.acnes.Additionally,compound 8 showed the capacity to suppress mitochondrial reactive oxygen species(ROS)production and nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)inflammasome activation,thereby reducing IL-1βmaturation and secretion.A three-dimensional quantitative structure-activity relationships(3D-QSAR)model was applied to compounds 5−12 to analyze their anti-inflammatory structure-activity relationships.
基金supported by the National Natural Science Foundation of China(No.U2333218).
文摘Airport disruptions often pose challenges in assigning aircraft to gates,resulting in infeasible planned schedules.In particular,a large number of transfer passengers miss their connections in the context of disruptions,which cause huge economic losses to airlines and serious passengers’dissatisfaction.This paper proposes a set-partitioning-based model to optimize Aircraft-Gate Reassignment with Transfer Passenger Connections(AGRP-TPC),which incorporates flexible gate-swap and aircraft-delay operations to mitigate the overall impact of disruptions.To efficiently solve the model,we introduce the concepts of additive-transfer and nonstop-transfer to handle passenger connections,and develop a Hierarchical Column-and-Row Generation(HCRG)approach guided by airport terminal space attribute.The column generation and row generation procedures solve iteratively until no new variables and constraints are generated.In addition,a follow-on strategy and a diving heuristic are designed to efficiently obtain high-quality solutions.We evaluate the proposed approach using various instances from a major Chinese international airport.Computational results demonstrate that our approach outperforms the comparison algorithms and produces good solutions within the time limit.Detailed results indicate that our approach effectively reduces overall losses in aircraft-gate reassignment following disruptions,and it can serve as an auxiliary decision-making tool for airport operators and airlines.
基金Specialized Science Project for Public Welfare Industries(Metrological Sector)(GYHY201206010,GYHY201406009)Science and Technology Planning Project for Guangdong Province(2012A061400012)+3 种基金Program for the 12th Five-Year Economic Development(2012BAC22B00)Natural Science Foundation of China(41075083)Program for Integration and Application of Key Meteorological Techniques from CMA(CMAGJ2012M36)Project from Guangdong Meteorological Bureau(2013A04)
文摘In this paper, we first analyzed cloud drift wind(CDW) data distribution in the vertical direction, and then reassigned the height of every CDW in the research domain in terms of background information, and finally, conducted contrast numerical experiments of assimilating the CDW data before and after reassignment to examine the impacts on the forecast of the track of Typhoon Chanthu(1003) from 00:00(Coordinated Universal Time) 21 July to 00:00 UTC23 July, 2010. The analysis results of the CDW data indicate that the number of CDWs is mainly distributed in the midand upper-troposphere above 500 h Pa, with the maximum number at about 300 h Pa. The height reassigning method mentioned in this work may update the height effectively, and the CDW data are distributed reasonably and no obvious contradiction occurs in the horizontal direction after height reassignment. After assimilating the height-reassigned CDW data, especially the water vapor CDW data, the initial wind field around Typhoon Chanthu(1003) became more reasonable, and then the steering current leading the typhoon to move to the correct location became stronger. As a result, the numerical track predictions are improved.
文摘A group of 39 Transgender subjects, 17 Female to Male (FtM) and 22 Male to Female (MtF), have been evaluated about their sexual desire, activity, and satisfaction during the period before and after Sex Reassignment by Surgery (SRS). Visual Analogue Scale from 0 to 10 has been used to assess sexual desire and satisfaction, number of sexual partners and number of sexual intercourses/ month have been evaluated to assess sexual activity. Moreover, after the surgery, the Sexual Desire Inventory-2 (SDI-2) was administered to patients and related to testosterone (total or free) levels. In FtM, after hysterectomy and bilateral ovariectomy, mean value of sexual desire (VAS 0 - 10) was 7.5 before beginning testosterone therapy, this value increased after six months of therapy and after one year. Just before SRS the value decreased, but increased to 8.42 after SRS. In the same patients the values (VAS 0 - 10) on sexual satisfaction were respectively 6.5, 6.9, 7.1, 6.5 and 7. Mean number of partners was 4, with 7.07 mean frequency of sexual intercourse/month, before starting therapy (after hysterectomy). During hormonal therapy (testosterone), No. of partners was 2.46 and 8.96 intercourses/month, while after SRS the partners were 1.25 and intercouses/month 7.91. MtF patients reported a sexual desire (VAS 0 - 10) before therapy of 7, this remained almost constant after 6 months, decreases after 12 months and just before surgery, and finally increased to 7.76 after SRS. For sexual satisfaction mean values of VAS were respectively: 6.1, 6.2, 6.3, 6, and 6.8. Regarding sexual activity, MtF report 3.81 mean No. of partners and 9.27 intercouses/month before the beginning of hormonal therapy, 1.36 partners and 4.36 intercourses after therapy, and 1.3 partners and 4.54 intercourses after SRS.
文摘We present a case of a transsexual patient who underwent a partial pelvectomy and genital reconstruction for anal cancer after chemoradiation. This is the first case in literature reporting on the occurrence of anal cancer after male-to-female sex reassignment surgery. We describe the surgical approach presenting our technique to avoid postoperative complications and preserve the sexual reassignment.
基金supported by the National Natural Science Foundation of China(62005116 and 51720105015)the Science and Technology Innovation Commission of Shenzhen(KQTD20170810110913065 and 20200925174735005).
文摘In light-sheet fluorescence microscopy,the axial resolution and field of view are mutually constrained.Axially swept light-sheet microscopy(ASLM)can decouple the trade-off,but the confocal detection scheme using a rolling shutter also rejects fluorescence signals from the specimen in the field of interest,which sacrifices the photon efficiency.Here,we report a laterally swept light-sheet microscopy(LSLM)scheme in which the focused beam is first scanned along the axial direction and subsequently laterally swept with the rolling shutter.We show that LSLM can obtain a higher photon efficiency when similar axial resolution and field of view can be achieved.Moreover,based on the principle of image scanning microscopy,applying the pixel reassignment to the LSLM images,hereby named iLSLM,improves the optical sectioning.Both simulation and experimental results demonstrate the higher photon efficiency with similar axial resolution and optical sectioning.Our proposed scheme is suitable for volumetric imaging of specimens that are susceptible to photobleaching or phototoxicity.
