[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []V...[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients （FF） of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.展开更多
Genetic algorithms offer very good performances for solving large optimization problems, especially in the domain of error-correcting codes. However, they have a major drawback related to the time complexity and memor...Genetic algorithms offer very good performances for solving large optimization problems, especially in the domain of error-correcting codes. However, they have a major drawback related to the time complexity and memory occupation when running on a uniprocessor computer. This paper proposes a parallel decoder for linear block codes, using parallel genetic algorithms (PGA). The good performance and time complexity are confirmed by theoretical study and by simulations on BCH(63,30,14) codes over both AWGN and flat Rayleigh fading channels. The simulation results show that the coding gain between parallel and single genetic algorithm is about 0.7 dB at BER = 10﹣5 with only 4 processors.展开更多
A memory and driving clock efficient design scheme to achieve WCDMA high-speed channel decoder on a single XILINX’ XVC1000E FPGA chip is presented. Using a modified MAP algorithm, say parallel Sliding Window logarith...A memory and driving clock efficient design scheme to achieve WCDMA high-speed channel decoder on a single XILINX’ XVC1000E FPGA chip is presented. Using a modified MAP algorithm, say parallel Sliding Window logarithmic Maximum A Posterior (PSW-log-MAP), the on-chip turbo decoder can decode an information bit by only an average of two clocks per iteration. On the other hand, a high-parallel pipeline Viterbi algorithm is adopted to realize the 256-state convolutional code decoding. The final decoder with an 8×chip-clock (30 72MHz) driving can concurrently process a data rate up to 2 5Mbps of turbo coded sequences and a data rate over 400kbps of convolutional codes. There is no extern memory needed. Test results show that the decoding performance is only 0 2～0 3dB or less lost comparing to float simulation.展开更多
正交时频空(Orthogonal Time Frequency Space,OTFS)调制因其在高多普勒频偏环境下的可靠传输能力,已成为低轨卫星等高动态通信场景的关键技术。然而,作为多载波调制技术,OTFS信号的高峰均功率比(Peak-to-Average Power Ratio,PAPR)易...正交时频空(Orthogonal Time Frequency Space,OTFS)调制因其在高多普勒频偏环境下的可靠传输能力,已成为低轨卫星等高动态通信场景的关键技术。然而,作为多载波调制技术,OTFS信号的高峰均功率比(Peak-to-Average Power Ratio,PAPR)易导致功放进入非线性工作状态,产生信号失真,影响通信可靠性和稳定性。格雷互补序列因其特殊的定义,使得该序列的最大峰均比不超过3 dB。基于里德-穆勒(Reed-Muller,RM)编码与格雷互补序列之间的特殊联系,提出了一种基于RM编码的OTFS系统的峰均功率比抑制方法。在发射端,首先利用RM编码将原始比特流序列编码转换为格雷互补序列形式,再进行星座映射与OTFS调制,得到低峰均功率比的发射信号。在接收端,为了实现对这种特殊编码信号的准确译码,设计了一种两步级联译码算法,通过陪集选择译码与单项式系数译码的级联实现了对具有格雷互补序列的RM编码的纠错译码,保证了通信传输的可靠性。仿真结果表明,在低轨卫星通信场景下,该编码方法可以将OTFS系统发射信号的峰均功率比抑制在3 dB以内;相较于OFDM系统,OTFS系统具有更强的鲁棒性;两步级联译码算法实现了较高信噪比(>6 dB)下更高的传输可靠性。上述方案的提出不仅为OTFS调制技术在星地高动态通信场景中的应用提供了有力的技术支持,也为未来多载波调制信号的峰均比抑制提供了新的参考。展开更多
Parallel concatenated spa ce time trellis code modulation, called Turbo STCM, can efficiently increase the coding gains of the space time codes. However, the complexity of the iterat iv e decoding restricts its ap...Parallel concatenated spa ce time trellis code modulation, called Turbo STCM, can efficiently increase the coding gains of the space time codes. However, the complexity of the iterat iv e decoding restricts its application. This paper introduces a lower complex deco ding algorithm based on soft output Viterbi algorithm (SOVA) for Turbo STCM. S imulational results show that the new SOVA algorithm for the Turbo STCM outperf orms the original space time trellis code (STTC) by 4~6 dB. At the same time, compared with the Max Log MAP (maximum a posteriori) algorithm, the new scheme requires a lower complexity and approaches the performance of Turbo STCM decod ing w ith Max Log MAP.展开更多
Space time trellis coding （STTC） techniques have been proposed to achieve both diversity and coding gains in multiple input multiple output （MIMO） fading channels. But with more transmit antennas STTCs suffer from...Space time trellis coding （STTC） techniques have been proposed to achieve both diversity and coding gains in multiple input multiple output （MIMO） fading channels. But with more transmit antennas STTCs suffer from the design dificulty and complexity increasing. This paper proposes a scheme, named parallel concatenated space time trellis codes （PC-STTC）, to achieve the tradeoff between the performances and complexity of STTCs for a large number of transmit antennas. Simulation results and complexity comparison are provided to demonstrate the performance and superiority of the proposed scheme over conventional schemes in fast fading channels in low signal-to-noise ratio （SNR） regions. And an EXIT （extrinsic information transform） chart is given to analyze the iterative convergence of the proposed scheme. It shows that PC-STTC has better iterative convergence in low SNR regions.展开更多
Recently, space time block codes (STBCs) are proposed for multi-input and multi-output (MIMO) antenna systems. Designing an STBC with both low decoding complexity and non-vanishing property for the Long Term Evolution...Recently, space time block codes (STBCs) are proposed for multi-input and multi-output (MIMO) antenna systems. Designing an STBC with both low decoding complexity and non-vanishing property for the Long Term Evolution Advanced (LTE-A) remains an open issue. In this paper, first our previously proposed STBC’s non-vanishing property will be completely described. The proposed STBC scheme has some interesting properties: 1) the scheme can achieve full rate and full diversity;2) its maximum likelihood (ML) decoding requires a joint detection of three real symbols;3) the minimum determinant values (MDVs) do not vanish by increasing signal constellation sizes;4) compatible with the single antenna transmission mode. The sentence has been dropped. Second, in order to improve BER performance, we propose a variant of proposed STBC. This scheme further decreases the detection complexity with a rate reduction of 33%;moreover, non-vanishing MDVs property is preserved. The simulation results show the second proposed STBC has better BER performance compared with other schemes.展开更多
Overlapped time domain multiplexing(OvTDM)is an innovative encoding scheme that can obtain high spectral efficiency.However,the intentional inter-symbol interference(ISI)caused by OvTDM will make the decoding process ...Overlapped time domain multiplexing(OvTDM)is an innovative encoding scheme that can obtain high spectral efficiency.However,the intentional inter-symbol interference(ISI)caused by OvTDM will make the decoding process more complex.The computational complexity of maximum likelihood sequence detection increases exponentially with the growth of spectral efficiency in OvTDM.As a consequence of high complexity,the decoding effort for a given spectral efficiency may occasionally exceed the physical limitations of the decoder,leading inevitably to buffer overflows and information erasures.In this paper,we propose a bidirectional Viterbi algorithm(BVA)based on the bidirectional sequence decoding for OvTDM.With the BVA,the decoding operation starts simultaneously from the both ends of the corresponding trellis and stops at the middle of trellis.The simulation results show that compared with Viterbi algorithm(VA),the decoding time of BVA can be reduced by about half.And the memory space of two decoders in BVA are about half of that in VA,which means that the BVA has lower memory requirements for decoder.And the decoding performance of BVA is almost the same as VA.展开更多
Considering the influence of more random atmospheric turbulence, worse pointing errors and highly dynamic link on the transmission performance of mobile multiple-input multiple-output （MIMO） free space optics （FSO...Considering the influence of more random atmospheric turbulence, worse pointing errors and highly dynamic link on the transmission performance of mobile multiple-input multiple-output （MIMO） free space optics （FSO） communica- tion systems, this paper establishes a channel model for the mobile platform. Based on the combination of Alamouti space-time code and time hopping ultra-wide band （TH-UWB） communications, a novel repetition space-time coding （RSTC） method for mobile 2x2 free-space optical communications with pulse position modulation （PPM） is devel- oped. In particular, two decoding methods of equal gain combining （EGC） maximum likelihood detection （MLD） and correlation matrix detection （CMD） are derived. When a quasi-static fading and weak turbulence channel model are considered, simulation results show that whether the channel state information （CSI） is known or not, the coding sys- tem demonstrates more significant performance of the symbol error rate （SER） than the uncoding. In other words, transmitting diversity can be achieved while conveying the information only through the time delays of the modulated signals transmitted from different antennas. CMD has almost the same effect of signal combining with maximal ratio combining （MRC）. However, when the channel correlation increases, SER performance of the coding 2×2 system de- grades significantly.展开更多
Some specified chips in traditional Manchester-Ⅱencoding/decoding designs are used to guarantee strictly the stability of the input wave,otherwise the capacity of anti-interference and resilience are degraded serious...Some specified chips in traditional Manchester-Ⅱencoding/decoding designs are used to guarantee strictly the stability of the input wave,otherwise the capacity of anti-interference and resilience are degraded seriously.In this paper,a new Manchester-Ⅱencoding/ decoding system is used for nuclear logging by a 7 000 m armoring cable.A thorough hardware wave tracking decoding algorithm is proposed and realized in a FPGA hardware chip.An on-site measurements show that this transmission system can decode correctly in real time,with a bit error rate of better than 10^(-10).展开更多
Multiple antenna wireless systems can provide larger channel capacity and enable spatial diversity to combat fading. In this paper we conduct an investigation into the design of coded space-time system obtained by ser...Multiple antenna wireless systems can provide larger channel capacity and enable spatial diversity to combat fading. In this paper we conduct an investigation into the design of coded space-time system obtained by serially concatenating channel code module and space-time code module with an interleaver in between. As an example, the system is constructed by employing low decoding complexity turbo-SPC （single parity check） code as outer module and linear complex field space-time code as inner module, which achieves full diversity and lossless equivalent channel capacity. Simulation results prove that our designed system performs well and it only loses 0.8 dB from multiple-input multiple-output （MIMO） capacity at BER = 10^-5 in the case of information bit length 6048. Compared with turbo code-based systems, it also has lower error floor.展开更多
The matrix inversion operation is needed in the MMSE decoding algorithm of orthogonal space-time block coding （OSTBC） proposed by Papadias and Foschini. In this paper, an minimum mean square error （MMSE） decoding ...The matrix inversion operation is needed in the MMSE decoding algorithm of orthogonal space-time block coding （OSTBC） proposed by Papadias and Foschini. In this paper, an minimum mean square error （MMSE） decoding algorithm without matrix inversion is proposed, by which the computational complexity can be reduced directly but the decoding performance is not affected.展开更多
This paper presents a low complexity algorithm for the decoding of space-time block codes （STBC） under non-constant module modulation. Simulation results show that in most cases of non-constant module modulation the...This paper presents a low complexity algorithm for the decoding of space-time block codes （STBC） under non-constant module modulation. Simulation results show that in most cases of non-constant module modulation the new algorithm results in more than 50% reduction in computation complexity comparing to the original maximum likelihood algorithm without any loss of BER performance.展开更多
基金Supported by National Natural Science Foundation of China(31072155)Natural Science Foundation of Jiangsu Province(BK2010068)+1 种基金Fund for Independent Innovation of Agricultural Science in Jiangsu Province[CX(11)2060]Special Fund for Agroscientific Research in the Public Interest(201303041)~~
文摘[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients （FF） of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.
文摘Genetic algorithms offer very good performances for solving large optimization problems, especially in the domain of error-correcting codes. However, they have a major drawback related to the time complexity and memory occupation when running on a uniprocessor computer. This paper proposes a parallel decoder for linear block codes, using parallel genetic algorithms (PGA). The good performance and time complexity are confirmed by theoretical study and by simulations on BCH(63,30,14) codes over both AWGN and flat Rayleigh fading channels. The simulation results show that the coding gain between parallel and single genetic algorithm is about 0.7 dB at BER = 10﹣5 with only 4 processors.
文摘A memory and driving clock efficient design scheme to achieve WCDMA high-speed channel decoder on a single XILINX’ XVC1000E FPGA chip is presented. Using a modified MAP algorithm, say parallel Sliding Window logarithmic Maximum A Posterior (PSW-log-MAP), the on-chip turbo decoder can decode an information bit by only an average of two clocks per iteration. On the other hand, a high-parallel pipeline Viterbi algorithm is adopted to realize the 256-state convolutional code decoding. The final decoder with an 8×chip-clock (30 72MHz) driving can concurrently process a data rate up to 2 5Mbps of turbo coded sequences and a data rate over 400kbps of convolutional codes. There is no extern memory needed. Test results show that the decoding performance is only 0 2～0 3dB or less lost comparing to float simulation.
文摘正交时频空(Orthogonal Time Frequency Space,OTFS)调制因其在高多普勒频偏环境下的可靠传输能力,已成为低轨卫星等高动态通信场景的关键技术。然而,作为多载波调制技术,OTFS信号的高峰均功率比(Peak-to-Average Power Ratio,PAPR)易导致功放进入非线性工作状态,产生信号失真,影响通信可靠性和稳定性。格雷互补序列因其特殊的定义,使得该序列的最大峰均比不超过3 dB。基于里德-穆勒(Reed-Muller,RM)编码与格雷互补序列之间的特殊联系,提出了一种基于RM编码的OTFS系统的峰均功率比抑制方法。在发射端,首先利用RM编码将原始比特流序列编码转换为格雷互补序列形式,再进行星座映射与OTFS调制,得到低峰均功率比的发射信号。在接收端,为了实现对这种特殊编码信号的准确译码,设计了一种两步级联译码算法,通过陪集选择译码与单项式系数译码的级联实现了对具有格雷互补序列的RM编码的纠错译码,保证了通信传输的可靠性。仿真结果表明,在低轨卫星通信场景下,该编码方法可以将OTFS系统发射信号的峰均功率比抑制在3 dB以内;相较于OFDM系统,OTFS系统具有更强的鲁棒性;两步级联译码算法实现了较高信噪比(>6 dB)下更高的传输可靠性。上述方案的提出不仅为OTFS调制技术在星地高动态通信场景中的应用提供了有力的技术支持,也为未来多载波调制信号的峰均比抑制提供了新的参考。
文摘Parallel concatenated spa ce time trellis code modulation, called Turbo STCM, can efficiently increase the coding gains of the space time codes. However, the complexity of the iterat iv e decoding restricts its application. This paper introduces a lower complex deco ding algorithm based on soft output Viterbi algorithm (SOVA) for Turbo STCM. S imulational results show that the new SOVA algorithm for the Turbo STCM outperf orms the original space time trellis code (STTC) by 4~6 dB. At the same time, compared with the Max Log MAP (maximum a posteriori) algorithm, the new scheme requires a lower complexity and approaches the performance of Turbo STCM decod ing w ith Max Log MAP.
基金supported by Shanghai Municipal Government and Nokia
文摘Space time trellis coding （STTC） techniques have been proposed to achieve both diversity and coding gains in multiple input multiple output （MIMO） fading channels. But with more transmit antennas STTCs suffer from the design dificulty and complexity increasing. This paper proposes a scheme, named parallel concatenated space time trellis codes （PC-STTC）, to achieve the tradeoff between the performances and complexity of STTCs for a large number of transmit antennas. Simulation results and complexity comparison are provided to demonstrate the performance and superiority of the proposed scheme over conventional schemes in fast fading channels in low signal-to-noise ratio （SNR） regions. And an EXIT （extrinsic information transform） chart is given to analyze the iterative convergence of the proposed scheme. It shows that PC-STTC has better iterative convergence in low SNR regions.
文摘Recently, space time block codes (STBCs) are proposed for multi-input and multi-output (MIMO) antenna systems. Designing an STBC with both low decoding complexity and non-vanishing property for the Long Term Evolution Advanced (LTE-A) remains an open issue. In this paper, first our previously proposed STBC’s non-vanishing property will be completely described. The proposed STBC scheme has some interesting properties: 1) the scheme can achieve full rate and full diversity;2) its maximum likelihood (ML) decoding requires a joint detection of three real symbols;3) the minimum determinant values (MDVs) do not vanish by increasing signal constellation sizes;4) compatible with the single antenna transmission mode. The sentence has been dropped. Second, in order to improve BER performance, we propose a variant of proposed STBC. This scheme further decreases the detection complexity with a rate reduction of 33%;moreover, non-vanishing MDVs property is preserved. The simulation results show the second proposed STBC has better BER performance compared with other schemes.
文摘Overlapped time domain multiplexing(OvTDM)is an innovative encoding scheme that can obtain high spectral efficiency.However,the intentional inter-symbol interference(ISI)caused by OvTDM will make the decoding process more complex.The computational complexity of maximum likelihood sequence detection increases exponentially with the growth of spectral efficiency in OvTDM.As a consequence of high complexity,the decoding effort for a given spectral efficiency may occasionally exceed the physical limitations of the decoder,leading inevitably to buffer overflows and information erasures.In this paper,we propose a bidirectional Viterbi algorithm(BVA)based on the bidirectional sequence decoding for OvTDM.With the BVA,the decoding operation starts simultaneously from the both ends of the corresponding trellis and stops at the middle of trellis.The simulation results show that compared with Viterbi algorithm(VA),the decoding time of BVA can be reduced by about half.And the memory space of two decoders in BVA are about half of that in VA,which means that the BVA has lower memory requirements for decoder.And the decoding performance of BVA is almost the same as VA.
基金supported by the National Natural Science Foundation of China(No.61205106)
文摘Considering the influence of more random atmospheric turbulence, worse pointing errors and highly dynamic link on the transmission performance of mobile multiple-input multiple-output （MIMO） free space optics （FSO） communica- tion systems, this paper establishes a channel model for the mobile platform. Based on the combination of Alamouti space-time code and time hopping ultra-wide band （TH-UWB） communications, a novel repetition space-time coding （RSTC） method for mobile 2x2 free-space optical communications with pulse position modulation （PPM） is devel- oped. In particular, two decoding methods of equal gain combining （EGC） maximum likelihood detection （MLD） and correlation matrix detection （CMD） are derived. When a quasi-static fading and weak turbulence channel model are considered, simulation results show that whether the channel state information （CSI） is known or not, the coding sys- tem demonstrates more significant performance of the symbol error rate （SER） than the uncoding. In other words, transmitting diversity can be achieved while conveying the information only through the time delays of the modulated signals transmitted from different antennas. CMD has almost the same effect of signal combining with maximal ratio combining （MRC）. However, when the channel correlation increases, SER performance of the coding 2×2 system de- grades significantly.
基金Supported by the China National High Technology Research and Development Program(863 Plans) funding for this project(No,2006AA09A102-02)
文摘Some specified chips in traditional Manchester-Ⅱencoding/decoding designs are used to guarantee strictly the stability of the input wave,otherwise the capacity of anti-interference and resilience are degraded seriously.In this paper,a new Manchester-Ⅱencoding/ decoding system is used for nuclear logging by a 7 000 m armoring cable.A thorough hardware wave tracking decoding algorithm is proposed and realized in a FPGA hardware chip.An on-site measurements show that this transmission system can decode correctly in real time,with a bit error rate of better than 10^(-10).
基金supported by the National Natural Science Foundation of China （Grant Nos.60332030, 60572157）, and the National High-TechnologY Research and Development of China （Grant No.863-2003AA123310）
文摘Multiple antenna wireless systems can provide larger channel capacity and enable spatial diversity to combat fading. In this paper we conduct an investigation into the design of coded space-time system obtained by serially concatenating channel code module and space-time code module with an interleaver in between. As an example, the system is constructed by employing low decoding complexity turbo-SPC （single parity check） code as outer module and linear complex field space-time code as inner module, which achieves full diversity and lossless equivalent channel capacity. Simulation results prove that our designed system performs well and it only loses 0.8 dB from multiple-input multiple-output （MIMO） capacity at BER = 10^-5 in the case of information bit length 6048. Compared with turbo code-based systems, it also has lower error floor.
文摘The matrix inversion operation is needed in the MMSE decoding algorithm of orthogonal space-time block coding （OSTBC） proposed by Papadias and Foschini. In this paper, an minimum mean square error （MMSE） decoding algorithm without matrix inversion is proposed, by which the computational complexity can be reduced directly but the decoding performance is not affected.
基金Supported by the National 863 Plan Project (NO.2002AA123032)
文摘This paper presents a low complexity algorithm for the decoding of space-time block codes （STBC） under non-constant module modulation. Simulation results show that in most cases of non-constant module modulation the new algorithm results in more than 50% reduction in computation complexity comparing to the original maximum likelihood algorithm without any loss of BER performance.
