In the context of the continuous deepening of the“Double Reduction”policy and the growing demand for quality education,leveled mathematics readers,as an emerging form of publishing that integrates subject education ...In the context of the continuous deepening of the“Double Reduction”policy and the growing demand for quality education,leveled mathematics readers,as an emerging form of publishing that integrates subject education and reading experience,face challenges such as unclear leveling logic,insufficient functional support,and weak user engagement.This paper introduces the 4V marketing theory and constructs an analytical framework from four dimensions:differentiation,functionality,added value,and resonance.Two representative products,“Climbing Mathematics”and“Spark Mathematics,”are selected for a typical case comparison to identify their strengths and weaknesses in content design,service systems,and brand operation,and to extract transferable strategic elements.The study finds that the user-value-oriented strategy based on the 4V model can effectively address the core issues in the market promotion and user relationship building of leveled mathematics readers,providing practical paths and theoretical support for educational publishing institutions to achieve product innovation and brand upgrading in this niche field.展开更多
In sports timing systems,P2P communication is used at low frequency bandwidths(under 135 KHz) between tags and readers in the RFID field.However,in such cases,collisions tend to occur when a reader deals with multiple...In sports timing systems,P2P communication is used at low frequency bandwidths(under 135 KHz) between tags and readers in the RFID field.However,in such cases,collisions tend to occur when a reader deals with multiple RFID tags simultaneously.To overcome this issue,a sports timing system including a Multi Reader Controller(MRC)loaded with an advanced multiple reader algorithm and application was created and applied at large-scale citizens' marathon events.In these cases,a large number of people pass over the installed urethane type's antenna mat continually during a short period of time.This study verified the superiority of the improved algorithm and application through the on-thespot application of the multi reader algorithm and application program,which allows us to smoothly measure runners' times through multiaccess reading for rapid collision avoidance.展开更多
This paper mainly completed the design of hardware and software of RFID to read write system. Including selection and easy transplantation of open source μC/OS-Ⅱ operating system, completed the transplant in the emb...This paper mainly completed the design of hardware and software of RFID to read write system. Including selection and easy transplantation of open source μC/OS-Ⅱ operating system, completed the transplant in the embedded module, the task to create work; Analyze the system software level and concrete structure from the hardware layer, driver layer, operating system layer and user layer; and analyzes the influence of the actual the power consumption of the system running in different modes and the read-write distance, the influence of moving speed of chip on read and write time.展开更多
Reader proteins that bind specific methyllysine are important to biological functions of lysine methylation,but readers of many methyllysine sites are still unknown.Therefore,development of covalent probes is importan...Reader proteins that bind specific methyllysine are important to biological functions of lysine methylation,but readers of many methyllysine sites are still unknown.Therefore,development of covalent probes is important to identify readers from cell samples so as to understand biological roles of lysine methylation.Generally,readers bind methyllysine via aromatic cages that contain tryptophan,tyrosine and phenylalanine,that offer a unique motif for selective crosslinking.We recently reported a site-selective tryptophan crosslinking strategy based on dimethylsulfonium that mimics dimethyllysine to crosslink tryptophan in aromatic cages of readers.Since tyrosine is a key residue for binding affinity to methyllysine,especially some readers that do not contain tryptophan residues in the binding pocket.Here we developed strategies of site-selective crosslinking to tyrosine.Ultraviolet(UV)source was applied to excite tyrosine at neutral pH or phenoxide at basic p H,and subsequent single-electron transfer(SET)from Tyr*to sulfonium inside the binding pocket enables selective crosslinking.In consequence,methyllysine readers with tyrosine-containing aromatic cages could be selectively crosslinked by site-specific sulfonium peptide probes.In addition,we expanded substrates from aromatic cages to tyrosine residues of proximate contact with sulfonium probes.The pair of LgBiT and SmBiT exhibited orthogonal crosslinking in complicated cell samples.As a result,we may expand sulfonium tools to target local tyrosine in future investigations.展开更多
A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional G...A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional Gilbert mixer pair in commutating mode. A quadrature down-conversion mixer based on this topology is designed and optimized for an ultra high frequency RFID reader. Operating in the 915MHz ISM band, the presented quadrature mixer measures a conversion gain of 12.5dB,an IIP3 of 10dBm, an IIP2 of 58dBm, and an SSB noise figure of 17.6riB. The chip was fabricated in a 0. 18μm 1P6M RF CMOS process and consumes only 3mA of current from a 1.8V power supply.展开更多
A direct conversion receiver with optimized tolerance to local carrier interference is designed and implemented in a 0.18μm 1P6M mixed-signal CMOS process for a 900MHz RFID reader transceiver. A baseband amplifier wi...A direct conversion receiver with optimized tolerance to local carrier interference is designed and implemented in a 0.18μm 1P6M mixed-signal CMOS process for a 900MHz RFID reader transceiver. A baseband amplifier with series feedback topology is proposed to achieve passive mixer buffering,baseband DC cancellation,and signal amplification simultaneously. The receiver has a measured input ldB compression point of - 4dBm and a sensitivity of - 70dBm when 10dB SNR for digital demodulation is required. The receiver is integrated in a reader transceiver chip and consumes 90mA from a 1.8V supply.展开更多
Henry James' s first novella Daisy Miller brought him popularity and literary reputation after its publication. Through the application of reader theory and a close reading of the text, this article analyzes Daisy...Henry James' s first novella Daisy Miller brought him popularity and literary reputation after its publication. Through the application of reader theory and a close reading of the text, this article analyzes Daisy's strong personality and her misfortunes during her trip to Europe, and reveals how the novella highlights the conflict between American frankness and innocence and European sophistication. Besides, this article also elaborates on James' unique artistic skills—central consciousness and psychological analysis through his choice of narrative point of view, which contribute to the theme and the artistic charm of DaisyMiller.展开更多
A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration...A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading was conducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity （R2 = 0.9971）, when the inhibition rate was in the range of 10-90%. The lower limit of detection （LLD, at 99.7% probability） and the biological limit of detection （BLD, at 99.7% probability） of the method were 10.643 and 40 mg/mL, respectively. The repeatability standard deviation （R.S.D.） was 1.08%. The standard deviation of the method was ±0.027 AT-U.展开更多
AIM： To determine the performance of novice readers （4^th year medical students） for detecting capsule endoscopy findings. METHODS： Ten capsule endoscopy cases of small bowel lesions were administered to the reade...AIM： To determine the performance of novice readers （4^th year medical students） for detecting capsule endoscopy findings. METHODS： Ten capsule endoscopy cases of small bowel lesions were administered to the readers. Gold standard findings were pre-defined by gastroenterologists. Ten gold standard ＂targets＂ were identified among the 10 cases. Readers were given a 30-min overview of Rapid Reader software and instructed to mark any potential areas of abnormalities. A software program was developed using SAS to analyze the thumbnailed findings. RESULTS： The overall sensitivity for detecting the gold standard findings was 80%. As a group, at least 5 out of 10 readers detected each gold standard finding per recording. All the gold standard targets were identified when the readers＇ results were combined. Incidental finding/false positive rate ranged between 8.2-59.8 per reader. CONCLUSION： A panel of medical students with minimal endoscopic experience can achieve high sensitivity in detecting lesions on capsule endoscopy. A group of novice readers can pre-screen recordings to thumbnail potential areas of small bowel lesions for further review. These thumbnails must be reviewed to determine the clinical relevance. Further studies are ongoing to assess other cohorts.展开更多
文摘In the context of the continuous deepening of the“Double Reduction”policy and the growing demand for quality education,leveled mathematics readers,as an emerging form of publishing that integrates subject education and reading experience,face challenges such as unclear leveling logic,insufficient functional support,and weak user engagement.This paper introduces the 4V marketing theory and constructs an analytical framework from four dimensions:differentiation,functionality,added value,and resonance.Two representative products,“Climbing Mathematics”and“Spark Mathematics,”are selected for a typical case comparison to identify their strengths and weaknesses in content design,service systems,and brand operation,and to extract transferable strategic elements.The study finds that the user-value-oriented strategy based on the 4V model can effectively address the core issues in the market promotion and user relationship building of leveled mathematics readers,providing practical paths and theoretical support for educational publishing institutions to achieve product innovation and brand upgrading in this niche field.
文摘In sports timing systems,P2P communication is used at low frequency bandwidths(under 135 KHz) between tags and readers in the RFID field.However,in such cases,collisions tend to occur when a reader deals with multiple RFID tags simultaneously.To overcome this issue,a sports timing system including a Multi Reader Controller(MRC)loaded with an advanced multiple reader algorithm and application was created and applied at large-scale citizens' marathon events.In these cases,a large number of people pass over the installed urethane type's antenna mat continually during a short period of time.This study verified the superiority of the improved algorithm and application through the on-thespot application of the multi reader algorithm and application program,which allows us to smoothly measure runners' times through multiaccess reading for rapid collision avoidance.
文摘This paper mainly completed the design of hardware and software of RFID to read write system. Including selection and easy transplantation of open source μC/OS-Ⅱ operating system, completed the transplant in the embedded module, the task to create work; Analyze the system software level and concrete structure from the hardware layer, driver layer, operating system layer and user layer; and analyzes the influence of the actual the power consumption of the system running in different modes and the read-write distance, the influence of moving speed of chip on read and write time.
基金the support from National Natural Science Foundation of China(No.22161132006)Key R&D Program of Zhejiang(No.2024SSYS0036)Westlake University Startup。
文摘Reader proteins that bind specific methyllysine are important to biological functions of lysine methylation,but readers of many methyllysine sites are still unknown.Therefore,development of covalent probes is important to identify readers from cell samples so as to understand biological roles of lysine methylation.Generally,readers bind methyllysine via aromatic cages that contain tryptophan,tyrosine and phenylalanine,that offer a unique motif for selective crosslinking.We recently reported a site-selective tryptophan crosslinking strategy based on dimethylsulfonium that mimics dimethyllysine to crosslink tryptophan in aromatic cages of readers.Since tyrosine is a key residue for binding affinity to methyllysine,especially some readers that do not contain tryptophan residues in the binding pocket.Here we developed strategies of site-selective crosslinking to tyrosine.Ultraviolet(UV)source was applied to excite tyrosine at neutral pH or phenoxide at basic p H,and subsequent single-electron transfer(SET)from Tyr*to sulfonium inside the binding pocket enables selective crosslinking.In consequence,methyllysine readers with tyrosine-containing aromatic cages could be selectively crosslinked by site-specific sulfonium peptide probes.In addition,we expanded substrates from aromatic cages to tyrosine residues of proximate contact with sulfonium probes.The pair of LgBiT and SmBiT exhibited orthogonal crosslinking in complicated cell samples.As a result,we may expand sulfonium tools to target local tyrosine in future investigations.
文摘A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional Gilbert mixer pair in commutating mode. A quadrature down-conversion mixer based on this topology is designed and optimized for an ultra high frequency RFID reader. Operating in the 915MHz ISM band, the presented quadrature mixer measures a conversion gain of 12.5dB,an IIP3 of 10dBm, an IIP2 of 58dBm, and an SSB noise figure of 17.6riB. The chip was fabricated in a 0. 18μm 1P6M RF CMOS process and consumes only 3mA of current from a 1.8V power supply.
基金the Science and Technology Commission of Shanghai Municipality(No.057062010)the EU BRIDGE Project(No.033546)~~
文摘A direct conversion receiver with optimized tolerance to local carrier interference is designed and implemented in a 0.18μm 1P6M mixed-signal CMOS process for a 900MHz RFID reader transceiver. A baseband amplifier with series feedback topology is proposed to achieve passive mixer buffering,baseband DC cancellation,and signal amplification simultaneously. The receiver has a measured input ldB compression point of - 4dBm and a sensitivity of - 70dBm when 10dB SNR for digital demodulation is required. The receiver is integrated in a reader transceiver chip and consumes 90mA from a 1.8V supply.
文摘Henry James' s first novella Daisy Miller brought him popularity and literary reputation after its publication. Through the application of reader theory and a close reading of the text, this article analyzes Daisy's strong personality and her misfortunes during her trip to Europe, and reveals how the novella highlights the conflict between American frankness and innocence and European sophistication. Besides, this article also elaborates on James' unique artistic skills—central consciousness and psychological analysis through his choice of narrative point of view, which contribute to the theme and the artistic charm of DaisyMiller.
文摘A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading was conducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity （R2 = 0.9971）, when the inhibition rate was in the range of 10-90%. The lower limit of detection （LLD, at 99.7% probability） and the biological limit of detection （BLD, at 99.7% probability） of the method were 10.643 and 40 mg/mL, respectively. The repeatability standard deviation （R.S.D.） was 1.08%. The standard deviation of the method was ±0.027 AT-U.
基金Supported by NIH Clinical Associate Physician(CAP)Award(Dr.Jutabha),American Society for Gastrointestinal Endoscopy Research Award(Dr.Jutabha),American College of Gastroenterology Capsule Endoscopy Research Award(Dr.Jutabha),NIH General Clinical Research
文摘AIM： To determine the performance of novice readers （4^th year medical students） for detecting capsule endoscopy findings. METHODS： Ten capsule endoscopy cases of small bowel lesions were administered to the readers. Gold standard findings were pre-defined by gastroenterologists. Ten gold standard ＂targets＂ were identified among the 10 cases. Readers were given a 30-min overview of Rapid Reader software and instructed to mark any potential areas of abnormalities. A software program was developed using SAS to analyze the thumbnailed findings. RESULTS： The overall sensitivity for detecting the gold standard findings was 80%. As a group, at least 5 out of 10 readers detected each gold standard finding per recording. All the gold standard targets were identified when the readers＇ results were combined. Incidental finding/false positive rate ranged between 8.2-59.8 per reader. CONCLUSION： A panel of medical students with minimal endoscopic experience can achieve high sensitivity in detecting lesions on capsule endoscopy. A group of novice readers can pre-screen recordings to thumbnail potential areas of small bowel lesions for further review. These thumbnails must be reviewed to determine the clinical relevance. Further studies are ongoing to assess other cohorts.
