The strict and efficient transcription termination is required to prevent production of aberrant read- through transcripts that may cause transcriptional interference at neighboring genes. However, the exact regulator...The strict and efficient transcription termination is required to prevent production of aberrant read- through transcripts that may cause transcriptional interference at neighboring genes. However, the exact regulatory mechanism remains poorly understood. Through a genetic screening of a LUCIFERASE （LUC） reporter system, we found that Arabidopsis nucleosome remodeler DECREASE IN DNA METHYL- ATION1 （DDM1） is a key component of this regulatory machinery and plays an important role in tran- scription termination, thus limiting transcriptional read-through （TRT）. By whole-genome strand- specific RNA sequencing, we identified and confirmed 43 endogenous TRTs between genes, transposable elements （TEs）, or genes and TEs in the ddml-10 mutant, which mainly occurred at heterochromatin regions. The DNA methylation analysis of these TRT regions revealed that TRT occurred frequently at the intergenic regions with a higher methylation level in wild type comparing to the regions where TRT did not occur. Our results suggest that the level of intergenic DNA methylation may involve in preventing aberrant gene TRT or producing new gene during evolution.展开更多
The cDNA of BYDV-GAV read-through protein(RTP)gene was amplified from the extracted RNA of BYDV-GAV by using the polymerase chain reaction(PCR),and cloned into pGEM-7zf(+).Its complete nucleotide sequence was determin...The cDNA of BYDV-GAV read-through protein(RTP)gene was amplified from the extracted RNA of BYDV-GAV by using the polymerase chain reaction(PCR),and cloned into pGEM-7zf(+).Its complete nucleotide sequence was determined by dideoxynucleotide chain-termination method.The BYDV-GAV RTP gene consists of 1377nt.Its sequences were most similar to that of the RTP gene of BYDV-MAV with identities of 87.4%and 87.1%at the nucleotide and amino acid levels,respectively.展开更多
基金supported by Foundation for Innovative Research Group of the National Natural Science Foundation of China(No.31121002)
文摘The strict and efficient transcription termination is required to prevent production of aberrant read- through transcripts that may cause transcriptional interference at neighboring genes. However, the exact regulatory mechanism remains poorly understood. Through a genetic screening of a LUCIFERASE （LUC） reporter system, we found that Arabidopsis nucleosome remodeler DECREASE IN DNA METHYL- ATION1 （DDM1） is a key component of this regulatory machinery and plays an important role in tran- scription termination, thus limiting transcriptional read-through （TRT）. By whole-genome strand- specific RNA sequencing, we identified and confirmed 43 endogenous TRTs between genes, transposable elements （TEs）, or genes and TEs in the ddml-10 mutant, which mainly occurred at heterochromatin regions. The DNA methylation analysis of these TRT regions revealed that TRT occurred frequently at the intergenic regions with a higher methylation level in wild type comparing to the regions where TRT did not occur. Our results suggest that the level of intergenic DNA methylation may involve in preventing aberrant gene TRT or producing new gene during evolution.
基金This work is supported by the National Key Basic Research(Project 973 No.G2000016201)National Natural Science Foundation of China(39970034).
文摘The cDNA of BYDV-GAV read-through protein(RTP)gene was amplified from the extracted RNA of BYDV-GAV by using the polymerase chain reaction(PCR),and cloned into pGEM-7zf(+).Its complete nucleotide sequence was determined by dideoxynucleotide chain-termination method.The BYDV-GAV RTP gene consists of 1377nt.Its sequences were most similar to that of the RTP gene of BYDV-MAV with identities of 87.4%and 87.1%at the nucleotide and amino acid levels,respectively.
